Non-thermal plasma treatment altered gene expression profiling in non-small-cell lung cancer A549 cells
Recent technological advances in atmospheric plasmas have made the creation of non-thermal atmospheric pressure plasma (NTP) possible for utilization in the medical field. Although accumulated evidence suggests that NTP induces cell death in various cancer cell types thus offering a promising altern...
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Veröffentlicht in: | BMC genomics 2015-06, Vol.16 (1), p.435-435, Article 435 |
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creator | Hou, Jue Ma, Jie Yu, K N Li, Wei Cheng, Cheng Bao, Lingzhi Han, Wei |
description | Recent technological advances in atmospheric plasmas have made the creation of non-thermal atmospheric pressure plasma (NTP) possible for utilization in the medical field. Although accumulated evidence suggests that NTP induces cell death in various cancer cell types thus offering a promising alternative treatment strategy, the mechanism underlying its therapeutic effect is not fully understood.
We analyzed relevant signaling cascades associated with the tumor protein p53, in particular the cell cycle arrest, DNA damage as well as the underlying apoptosis pathways. Based on our results, the major effect from plasma exposure was found to be the activation of MAPK and p53 signaling pathways, resulting in changes in gene expression of MEKK, GADD, FOS and JUN. Finally, a significant modulation in expression of genes related to cellular proliferation and differentiation was observed.
Overall, the presented data of the tumor transcriptome helped identify the key players in modulated gene expression following exposure to plasma at the molecular level, and also helped interpret the downstream processes. The present work laid the foundation for further studies to clarify the roles of multiple pathways in plasma-induced biological processes. Further investigation of these genes in other cell lines may reveal comprehensive mechanisms of plasma induced effects. |
doi_str_mv | 10.1186/s12864-015-1644-8 |
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We analyzed relevant signaling cascades associated with the tumor protein p53, in particular the cell cycle arrest, DNA damage as well as the underlying apoptosis pathways. Based on our results, the major effect from plasma exposure was found to be the activation of MAPK and p53 signaling pathways, resulting in changes in gene expression of MEKK, GADD, FOS and JUN. Finally, a significant modulation in expression of genes related to cellular proliferation and differentiation was observed.
Overall, the presented data of the tumor transcriptome helped identify the key players in modulated gene expression following exposure to plasma at the molecular level, and also helped interpret the downstream processes. The present work laid the foundation for further studies to clarify the roles of multiple pathways in plasma-induced biological processes. Further investigation of these genes in other cell lines may reveal comprehensive mechanisms of plasma induced effects.</description><identifier>ISSN: 1471-2164</identifier><identifier>EISSN: 1471-2164</identifier><identifier>DOI: 10.1186/s12864-015-1644-8</identifier><identifier>PMID: 26116417</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Analysis ; Apoptosis - genetics ; Carcinoma, Non-Small-Cell Lung - genetics ; Cell Cycle Checkpoints - genetics ; Cell Differentiation - genetics ; Cell Proliferation - genetics ; DNA Damage - genetics ; Gene Expression Profiling - methods ; Genetic aspects ; Health aspects ; Humans ; Lung cancer, Non-small cell ; Lung Neoplasms - genetics ; Mitogen-Activated Protein Kinases ; Plasma Gases - pharmacology ; Signal Transduction - genetics ; Transcriptome - genetics ; Tumor Cells, Cultured ; Tumor proteins ; Tumor Suppressor Protein p53 - genetics</subject><ispartof>BMC genomics, 2015-06, Vol.16 (1), p.435-435, Article 435</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Hou et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c636t-8030d74fc825702bbab671366f270a0954cb0175e25c025b0019a107d353bb53</citedby><cites>FETCH-LOGICAL-c636t-8030d74fc825702bbab671366f270a0954cb0175e25c025b0019a107d353bb53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483225/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483225/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27926,27927,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26116417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hou, Jue</creatorcontrib><creatorcontrib>Ma, Jie</creatorcontrib><creatorcontrib>Yu, K N</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Cheng, Cheng</creatorcontrib><creatorcontrib>Bao, Lingzhi</creatorcontrib><creatorcontrib>Han, Wei</creatorcontrib><title>Non-thermal plasma treatment altered gene expression profiling in non-small-cell lung cancer A549 cells</title><title>BMC genomics</title><addtitle>BMC Genomics</addtitle><description>Recent technological advances in atmospheric plasmas have made the creation of non-thermal atmospheric pressure plasma (NTP) possible for utilization in the medical field. Although accumulated evidence suggests that NTP induces cell death in various cancer cell types thus offering a promising alternative treatment strategy, the mechanism underlying its therapeutic effect is not fully understood.
We analyzed relevant signaling cascades associated with the tumor protein p53, in particular the cell cycle arrest, DNA damage as well as the underlying apoptosis pathways. Based on our results, the major effect from plasma exposure was found to be the activation of MAPK and p53 signaling pathways, resulting in changes in gene expression of MEKK, GADD, FOS and JUN. Finally, a significant modulation in expression of genes related to cellular proliferation and differentiation was observed.
Overall, the presented data of the tumor transcriptome helped identify the key players in modulated gene expression following exposure to plasma at the molecular level, and also helped interpret the downstream processes. The present work laid the foundation for further studies to clarify the roles of multiple pathways in plasma-induced biological processes. Further investigation of these genes in other cell lines may reveal comprehensive mechanisms of plasma induced effects.</description><subject>Analysis</subject><subject>Apoptosis - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Cell Cycle Checkpoints - genetics</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Proliferation - genetics</subject><subject>DNA Damage - genetics</subject><subject>Gene Expression Profiling - methods</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Lung cancer, Non-small cell</subject><subject>Lung Neoplasms - genetics</subject><subject>Mitogen-Activated Protein Kinases</subject><subject>Plasma Gases - pharmacology</subject><subject>Signal Transduction - genetics</subject><subject>Transcriptome - genetics</subject><subject>Tumor Cells, Cultured</subject><subject>Tumor proteins</subject><subject>Tumor Suppressor Protein p53 - genetics</subject><issn>1471-2164</issn><issn>1471-2164</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkl9r1jAYxYs43D8_gDcS8EYvOpM0SdsbYQydg6Hgdh_S9GkXSZOatGN-e5_yzrEXRi_SPPmdQ044RfGO0TPGGvU5M94oUVImS6aEKJtXxRETNSs5bl8_-z8sjnP-TSmrGy7fFIdcMZyy-qgYf8RQLneQJuPJ7E2eDFkSmGWCsBDjF0jQkxECEHiYE-TsYiBzioPzLozEBRLQAWXelxa8J37FsTXBQiLnUrRkm-bT4mAwPsPbx_WkuP329fbie3n98_Lq4vy6tKpSS9nQiva1GCzes6a860ynalYpNfCaGtpKYTtMIYFLS7nsMFJrGK37SlZdJ6uT4svOdl67CXqLIZLxek5uMumvjsbp_ZPg7vQY77UQTcX5ZvDx0SDFPyvkRU8ubwlMgLhmzVTLeSskVYh-2KGj8aBdGCI62g3XmBsvLWpaIXX2AoVfD5OzMQA-JOwLPu0JkFngYRnNmrO-uvm1z7Ida1PMOcHwlJRRvVVE7yqisSJ6q4huUPP--RM9Kf53ovoHNXO1CQ</recordid><startdate>20150606</startdate><enddate>20150606</enddate><creator>Hou, Jue</creator><creator>Ma, Jie</creator><creator>Yu, K N</creator><creator>Li, Wei</creator><creator>Cheng, Cheng</creator><creator>Bao, Lingzhi</creator><creator>Han, Wei</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150606</creationdate><title>Non-thermal plasma treatment altered gene expression profiling in non-small-cell lung cancer A549 cells</title><author>Hou, Jue ; Ma, Jie ; Yu, K N ; Li, Wei ; Cheng, Cheng ; Bao, Lingzhi ; Han, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c636t-8030d74fc825702bbab671366f270a0954cb0175e25c025b0019a107d353bb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Apoptosis - genetics</topic><topic>Carcinoma, Non-Small-Cell Lung - genetics</topic><topic>Cell Cycle Checkpoints - genetics</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Proliferation - genetics</topic><topic>DNA Damage - genetics</topic><topic>Gene Expression Profiling - methods</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Lung cancer, Non-small cell</topic><topic>Lung Neoplasms - genetics</topic><topic>Mitogen-Activated Protein Kinases</topic><topic>Plasma Gases - pharmacology</topic><topic>Signal Transduction - genetics</topic><topic>Transcriptome - genetics</topic><topic>Tumor Cells, Cultured</topic><topic>Tumor proteins</topic><topic>Tumor Suppressor Protein p53 - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hou, Jue</creatorcontrib><creatorcontrib>Ma, Jie</creatorcontrib><creatorcontrib>Yu, K N</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Cheng, Cheng</creatorcontrib><creatorcontrib>Bao, Lingzhi</creatorcontrib><creatorcontrib>Han, Wei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale in Context: Science</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hou, Jue</au><au>Ma, Jie</au><au>Yu, K N</au><au>Li, Wei</au><au>Cheng, Cheng</au><au>Bao, Lingzhi</au><au>Han, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Non-thermal plasma treatment altered gene expression profiling in non-small-cell lung cancer A549 cells</atitle><jtitle>BMC genomics</jtitle><addtitle>BMC Genomics</addtitle><date>2015-06-06</date><risdate>2015</risdate><volume>16</volume><issue>1</issue><spage>435</spage><epage>435</epage><pages>435-435</pages><artnum>435</artnum><issn>1471-2164</issn><eissn>1471-2164</eissn><abstract>Recent technological advances in atmospheric plasmas have made the creation of non-thermal atmospheric pressure plasma (NTP) possible for utilization in the medical field. Although accumulated evidence suggests that NTP induces cell death in various cancer cell types thus offering a promising alternative treatment strategy, the mechanism underlying its therapeutic effect is not fully understood.
We analyzed relevant signaling cascades associated with the tumor protein p53, in particular the cell cycle arrest, DNA damage as well as the underlying apoptosis pathways. Based on our results, the major effect from plasma exposure was found to be the activation of MAPK and p53 signaling pathways, resulting in changes in gene expression of MEKK, GADD, FOS and JUN. Finally, a significant modulation in expression of genes related to cellular proliferation and differentiation was observed.
Overall, the presented data of the tumor transcriptome helped identify the key players in modulated gene expression following exposure to plasma at the molecular level, and also helped interpret the downstream processes. The present work laid the foundation for further studies to clarify the roles of multiple pathways in plasma-induced biological processes. Further investigation of these genes in other cell lines may reveal comprehensive mechanisms of plasma induced effects.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26116417</pmid><doi>10.1186/s12864-015-1644-8</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Analysis Apoptosis - genetics Carcinoma, Non-Small-Cell Lung - genetics Cell Cycle Checkpoints - genetics Cell Differentiation - genetics Cell Proliferation - genetics DNA Damage - genetics Gene Expression Profiling - methods Genetic aspects Health aspects Humans Lung cancer, Non-small cell Lung Neoplasms - genetics Mitogen-Activated Protein Kinases Plasma Gases - pharmacology Signal Transduction - genetics Transcriptome - genetics Tumor Cells, Cultured Tumor proteins Tumor Suppressor Protein p53 - genetics |
title | Non-thermal plasma treatment altered gene expression profiling in non-small-cell lung cancer A549 cells |
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