Loss of Faap20 Causes Hematopoietic Stem and Progenitor Cell Depletion in Mice Under Genotoxic Stress

20‐kDa FANCA‐associated protein (FAAP20) is a recently identified protein that associates with the Fanconi anemia (FA) core complex component, FANCA. FAAP20 contains a conserved ubiquitin‐binding zinc‐finger domain and plays critical roles in the FA‐BRCA pathway of DNA repair and genome maintenance....

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Veröffentlicht in:Stem cells (Dayton, Ohio) Ohio), 2015-07, Vol.33 (7), p.2320-2330
Hauptverfasser: Zhang, Tingting, Wilson, Andrew F., Mahmood Ali, Abdullah, Namekawa, Satoshi H., Andreassen, Paul R., Ruhikanta Meetei, Amom, Pang, Qishen
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container_end_page 2330
container_issue 7
container_start_page 2320
container_title Stem cells (Dayton, Ohio)
container_volume 33
creator Zhang, Tingting
Wilson, Andrew F.
Mahmood Ali, Abdullah
Namekawa, Satoshi H.
Andreassen, Paul R.
Ruhikanta Meetei, Amom
Pang, Qishen
description 20‐kDa FANCA‐associated protein (FAAP20) is a recently identified protein that associates with the Fanconi anemia (FA) core complex component, FANCA. FAAP20 contains a conserved ubiquitin‐binding zinc‐finger domain and plays critical roles in the FA‐BRCA pathway of DNA repair and genome maintenance. The function of FAAP20 in animals has not been explored. Here, we report that deletion of Faap20 in mice led to a mild FA‐like phenotype with defects in the reproductive and hematopoietic systems. Specifically, hematopoietic stem and progenitor cells (HSPCs) from Faap20−/− mice showed defects in long‐term multilineage reconstitution in lethally irradiated recipient mice, with milder phenotype as compared to HSPCs from Fanca−/− or Fancc−/− mice. Faap20−/− mice are susceptible to mitomycin C (MMC)‐induced pancytopenia. That is, acute MMC stress induced a significant progenitor loss especially the erythroid progenitors and megakaryocyte–erythrocyte progenitors in Faap20−/− mice. Furthermore, Faap20−/− HSPCs displayed aberrant cell cycle pattern during chronic MMC treatment. Finally, using Faap20−/− Fanca−/− double‐knockout mice, we demonstrated a possible dominant effect of FANCA in the interaction between FAAP20 and FANCA. This novel Faap20 mouse model may be valuable in studying the regulation of the FA pathway during bone marrow failure progress in FA patients. Stem Cells 2015;33:2320–2330
doi_str_mv 10.1002/stem.2048
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FAAP20 contains a conserved ubiquitin‐binding zinc‐finger domain and plays critical roles in the FA‐BRCA pathway of DNA repair and genome maintenance. The function of FAAP20 in animals has not been explored. Here, we report that deletion of Faap20 in mice led to a mild FA‐like phenotype with defects in the reproductive and hematopoietic systems. Specifically, hematopoietic stem and progenitor cells (HSPCs) from Faap20−/− mice showed defects in long‐term multilineage reconstitution in lethally irradiated recipient mice, with milder phenotype as compared to HSPCs from Fanca−/− or Fancc−/− mice. Faap20−/− mice are susceptible to mitomycin C (MMC)‐induced pancytopenia. That is, acute MMC stress induced a significant progenitor loss especially the erythroid progenitors and megakaryocyte–erythrocyte progenitors in Faap20−/− mice. Furthermore, Faap20−/− HSPCs displayed aberrant cell cycle pattern during chronic MMC treatment. Finally, using Faap20−/− Fanca−/− double‐knockout mice, we demonstrated a possible dominant effect of FANCA in the interaction between FAAP20 and FANCA. This novel Faap20 mouse model may be valuable in studying the regulation of the FA pathway during bone marrow failure progress in FA patients. 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subjects Amino Acyl-tRNA Synthetases - genetics
Amino Acyl-tRNA Synthetases - metabolism
Animals
Bone marrow
Cell cycle
Cell Differentiation
DNA Damage
Erythroid differentiation
Fanconi anemia
Fanconi Anemia - genetics
Fanconi Anemia - metabolism
Fanconi Anemia - mortality
Hematopoietic progenitors
Hematopoietic Stem Cells - metabolism
Humans
Mice
Mice, Inbred C57BL
Rodents
Stem Cells
Survival Analysis
title Loss of Faap20 Causes Hematopoietic Stem and Progenitor Cell Depletion in Mice Under Genotoxic Stress
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