Molecular Identification of a Newly Isolated Bacillus subtilis BI19 and Optimization of Production Conditions for Enhanced Production of Extracellular Amylase
A study was carried out with a newly isolated bacterial strain yielding extracellular amylase. The phylogenetic tree constructed on the basis of 16S rDNA gene sequences revealed this strain as clustered with the closest members of Bacillus sp. and identified as Bacillus subtilis BI19. The effect of...
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| description | A study was carried out with a newly isolated bacterial strain yielding extracellular amylase. The phylogenetic tree constructed on the basis of 16S rDNA gene sequences revealed this strain as clustered with the closest members of Bacillus sp. and identified as Bacillus subtilis BI19. The effect of various fermentation conditions on amylase production through shake-flask culture was investigated. Rice flour (1.25%) as a cheap natural carbon source was found to induce amylase production mostly. A combination of peptone and tryptone as organic and ammonium sulfate as inorganic nitrogen sources gave highest yield. Maximum production was obtained after 24 h of incubation at 37°C with an initial medium pH 8.0. Addition of surfactants like Tween 80 (0.25 g/L) and sodium lauryl sulfate (0.2 g/L) resulted in 28% and 15% increase in enzyme production, respectively. Amylase production was 3.06 times higher when optimized production conditions were used. Optimum reaction temperature and pH for crude amylase activity were 50°C and 6.0, respectively. The crude enzyme showed activity and stability over a fair range of temperature and pH. These results suggest that B. subtilis BI19 could be exploited for production of amylase at relatively low cost and time. |
| doi_str_mv | 10.1155/2015/859805 |
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Mizanur</creator><contributor>Gopinath, Subash C. B.</contributor><creatorcontrib>Dash, Biplab Kumar ; Sarker, Palash Kumar ; Rahman, M. Mizanur ; Gopinath, Subash C. B.</creatorcontrib><description>A study was carried out with a newly isolated bacterial strain yielding extracellular amylase. The phylogenetic tree constructed on the basis of 16S rDNA gene sequences revealed this strain as clustered with the closest members of Bacillus sp. and identified as Bacillus subtilis BI19. The effect of various fermentation conditions on amylase production through shake-flask culture was investigated. Rice flour (1.25%) as a cheap natural carbon source was found to induce amylase production mostly. A combination of peptone and tryptone as organic and ammonium sulfate as inorganic nitrogen sources gave highest yield. Maximum production was obtained after 24 h of incubation at 37°C with an initial medium pH 8.0. Addition of surfactants like Tween 80 (0.25 g/L) and sodium lauryl sulfate (0.2 g/L) resulted in 28% and 15% increase in enzyme production, respectively. Amylase production was 3.06 times higher when optimized production conditions were used. Optimum reaction temperature and pH for crude amylase activity were 50°C and 6.0, respectively. The crude enzyme showed activity and stability over a fair range of temperature and pH. These results suggest that B. subtilis BI19 could be exploited for production of amylase at relatively low cost and time.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2015/859805</identifier><identifier>PMID: 26180814</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Amylases - biosynthesis ; Amylases - genetics ; Bacillus ; Bacillus subtilis ; Bacillus subtilis - enzymology ; Bacillus subtilis - genetics ; Bacillus subtilis - growth & development ; Bacillus subtilis - isolation & purification ; Bacteria ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; Biotechnology ; Deoxyribonucleic acid ; DNA ; Enzymes ; Extracellular matrix ; Fermentation ; Genetic engineering ; Health aspects ; Host-bacteria relationships ; Hydrogen-Ion Concentration ; Laboratories ; Observations ; Optimization ; Phylogeny ; Studies ; Surfactants</subject><ispartof>BioMed research international, 2015-01, Vol.2015 (2015), p.1-9</ispartof><rights>Copyright © 2015 Biplab Kumar Dash et al.</rights><rights>COPYRIGHT 2015 John Wiley & Sons, Inc.</rights><rights>Copyright © 2015 Biplab Kumar Dash et al. Biplab Kumar Dash et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2015 Biplab Kumar Dash et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-c864ee3791adc737ca13a90bc47532e4aa471b9fbb7bbee403f29114f94194793</citedby><cites>FETCH-LOGICAL-c491t-c864ee3791adc737ca13a90bc47532e4aa471b9fbb7bbee403f29114f94194793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477212/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477212/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26180814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gopinath, Subash C. B.</contributor><creatorcontrib>Dash, Biplab Kumar</creatorcontrib><creatorcontrib>Sarker, Palash Kumar</creatorcontrib><creatorcontrib>Rahman, M. Mizanur</creatorcontrib><title>Molecular Identification of a Newly Isolated Bacillus subtilis BI19 and Optimization of Production Conditions for Enhanced Production of Extracellular Amylase</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>A study was carried out with a newly isolated bacterial strain yielding extracellular amylase. The phylogenetic tree constructed on the basis of 16S rDNA gene sequences revealed this strain as clustered with the closest members of Bacillus sp. and identified as Bacillus subtilis BI19. The effect of various fermentation conditions on amylase production through shake-flask culture was investigated. Rice flour (1.25%) as a cheap natural carbon source was found to induce amylase production mostly. A combination of peptone and tryptone as organic and ammonium sulfate as inorganic nitrogen sources gave highest yield. Maximum production was obtained after 24 h of incubation at 37°C with an initial medium pH 8.0. Addition of surfactants like Tween 80 (0.25 g/L) and sodium lauryl sulfate (0.2 g/L) resulted in 28% and 15% increase in enzyme production, respectively. Amylase production was 3.06 times higher when optimized production conditions were used. Optimum reaction temperature and pH for crude amylase activity were 50°C and 6.0, respectively. The crude enzyme showed activity and stability over a fair range of temperature and pH. These results suggest that B. subtilis BI19 could be exploited for production of amylase at relatively low cost and time.</description><subject>Amylases - biosynthesis</subject><subject>Amylases - genetics</subject><subject>Bacillus</subject><subject>Bacillus subtilis</subject><subject>Bacillus subtilis - enzymology</subject><subject>Bacillus subtilis - genetics</subject><subject>Bacillus subtilis - growth & development</subject><subject>Bacillus subtilis - isolation & purification</subject><subject>Bacteria</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Biotechnology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Enzymes</subject><subject>Extracellular matrix</subject><subject>Fermentation</subject><subject>Genetic engineering</subject><subject>Health aspects</subject><subject>Host-bacteria relationships</subject><subject>Hydrogen-Ion Concentration</subject><subject>Laboratories</subject><subject>Observations</subject><subject>Optimization</subject><subject>Phylogeny</subject><subject>Studies</subject><subject>Surfactants</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNksFu1DAQhiMEolXpiTuyxAWBlmZiJ44vSNvVAisVygHO1sRxuq4ce7ETyvZh-qw4bFkWLuCLPfI3vz0zf5Y9hfw1QFmeFTmUZ3Up6rx8kB0XFNisAgYP92dKj7LTGK_ztGqoclE9zo6KCuoUsePs7oO3Wo0WA1m12g2mMwoH4x3xHUHyUd_YLVlFb3HQLTlHZawdI4ljMxhrIjlfgSDoWnK5GUxvbve5n4JvR_UzWnjXmukUSecDWbo1OpXUDpCUsPw-BFQ6yU-fmfdbi1E_yR51aKM-vd9Psi9vl58X72cXl-9Wi_nFTDEBw0zVFdOacgHYKk65QqAo8kYxXtJCM0TGoRFd0_Cm0ZrltCsEAOsEA8G4oCfZm53uZmx63arUiYBWboLpMWylRyP_vHFmLa_8N8kY5wUUSeDFvUDwX0cdB9mbOFWDTvsxSuBpUGkCFf03WokkWTKY0Od_odd-DC51YqJyqKGs6t_UFVotjev81MlJVM5ZmYZO06uJerWjVPAxBt3tq4NcTl6Sk5fkzkuJfnbYkD37yzkJeLkD1sa1eGP-T00nRHd4AJc8T-P4Aazp220</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Dash, Biplab Kumar</creator><creator>Sarker, Palash Kumar</creator><creator>Rahman, M. 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Mizanur</au><au>Gopinath, Subash C. B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Identification of a Newly Isolated Bacillus subtilis BI19 and Optimization of Production Conditions for Enhanced Production of Extracellular Amylase</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>2015</volume><issue>2015</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>A study was carried out with a newly isolated bacterial strain yielding extracellular amylase. The phylogenetic tree constructed on the basis of 16S rDNA gene sequences revealed this strain as clustered with the closest members of Bacillus sp. and identified as Bacillus subtilis BI19. The effect of various fermentation conditions on amylase production through shake-flask culture was investigated. Rice flour (1.25%) as a cheap natural carbon source was found to induce amylase production mostly. A combination of peptone and tryptone as organic and ammonium sulfate as inorganic nitrogen sources gave highest yield. Maximum production was obtained after 24 h of incubation at 37°C with an initial medium pH 8.0. Addition of surfactants like Tween 80 (0.25 g/L) and sodium lauryl sulfate (0.2 g/L) resulted in 28% and 15% increase in enzyme production, respectively. Amylase production was 3.06 times higher when optimized production conditions were used. Optimum reaction temperature and pH for crude amylase activity were 50°C and 6.0, respectively. The crude enzyme showed activity and stability over a fair range of temperature and pH. These results suggest that B. subtilis BI19 could be exploited for production of amylase at relatively low cost and time.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>26180814</pmid><doi>10.1155/2015/859805</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amylases - biosynthesis Amylases - genetics Bacillus Bacillus subtilis Bacillus subtilis - enzymology Bacillus subtilis - genetics Bacillus subtilis - growth & development Bacillus subtilis - isolation & purification Bacteria Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Biotechnology Deoxyribonucleic acid DNA Enzymes Extracellular matrix Fermentation Genetic engineering Health aspects Host-bacteria relationships Hydrogen-Ion Concentration Laboratories Observations Optimization Phylogeny Studies Surfactants |
| title | Molecular Identification of a Newly Isolated Bacillus subtilis BI19 and Optimization of Production Conditions for Enhanced Production of Extracellular Amylase |
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