Epidemiology of carbapenem non-susceptible Pseudomonas aeruginosa isolates in Eastern Algeria
Carbapenem resistance among Pseudomonas aeruginosa has become a serious life-threatening problem due to the limited therapeutic options. In this study, we investigated the prevalence and the molecular epidemiology of carbapenem resistant Pseudomonas aeruginosa (CRPA) isolated from three hospitals in...
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| Veröffentlicht in: | Antimicrobial resistance & infection control 2015-06, Vol.4 (1), p.27-27, Article 27 |
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| creator | Meradji, Samah Barguigua, Abouddihaj Zerouali, Khalid Mazouz, Dekhil Chettibi, Houria Elmdaghri, Naima Timinouni, Mohammed |
| description | Carbapenem resistance among Pseudomonas aeruginosa has become a serious life-threatening problem due to the limited therapeutic options. In this study, we investigated the prevalence and the molecular epidemiology of carbapenem resistant Pseudomonas aeruginosa (CRPA) isolated from three hospitals in Annaba city, Algeria.
During the study period (January, 2012 to December, 2013), all patients infected by P. aeruginosa were considered as the potential study population. Antibiotic susceptibility testing was performed as recommended by the CLSI. Screening of carbapenemase producer isolates was performed by using imipenem-EDTA double-disk synergy test and modified Hodge test. CRPA isolates were tested for the presence of genes encoding β-lactamases, plasmid mediated quinolone resistance, aminoglycoside resistance and class 1 integrons were investigated by PCR and sequencing. The clonal relatedness among CRPA isolates was analyzed by pulsed-field gel electrophoresis method. The clinical data were collected to identify risk factors for CRPA carriage of P. aeruginosa infection.
The overall prevalence of CRPA was 18.75 %. The risk factors for carrying CRPA were the length of hospital stay (p = 0.04), co-infections with Staphylococcus aureus (p = 0.01), and the use of urinary catheter (p = 0.03). The in-hospital mortality rate among case patients was 13.33 % compared with 1.53 % for control patients (p = 0.09). All CRPA isolates were multidrug resistance and the most effective antibiotic against CRPA isolates was amikacin and colistin. PFGE revealed an epidemic clonal dissemination of CRPA isolates. None of CRPA isolated were found to be carbapenemase-producers. The bla PSE-1 and aac(3)-II gene was detected in two and five strains respectively. The class1 integrons were detected in 2 isolates with the presence of aadA7 gene cassette in these integrons.
The endemic clonal dissemination and multi-drug resistance of CRPA isolates in our institution is highly alarming. Strict measure will be required to control the further spread of these pathogens in hospital setting. |
| doi_str_mv | 10.1186/s13756-015-0067-2 |
| format | Article |
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During the study period (January, 2012 to December, 2013), all patients infected by P. aeruginosa were considered as the potential study population. Antibiotic susceptibility testing was performed as recommended by the CLSI. Screening of carbapenemase producer isolates was performed by using imipenem-EDTA double-disk synergy test and modified Hodge test. CRPA isolates were tested for the presence of genes encoding β-lactamases, plasmid mediated quinolone resistance, aminoglycoside resistance and class 1 integrons were investigated by PCR and sequencing. The clonal relatedness among CRPA isolates was analyzed by pulsed-field gel electrophoresis method. The clinical data were collected to identify risk factors for CRPA carriage of P. aeruginosa infection.
The overall prevalence of CRPA was 18.75 %. The risk factors for carrying CRPA were the length of hospital stay (p = 0.04), co-infections with Staphylococcus aureus (p = 0.01), and the use of urinary catheter (p = 0.03). The in-hospital mortality rate among case patients was 13.33 % compared with 1.53 % for control patients (p = 0.09). All CRPA isolates were multidrug resistance and the most effective antibiotic against CRPA isolates was amikacin and colistin. PFGE revealed an epidemic clonal dissemination of CRPA isolates. None of CRPA isolated were found to be carbapenemase-producers. The bla PSE-1 and aac(3)-II gene was detected in two and five strains respectively. The class1 integrons were detected in 2 isolates with the presence of aadA7 gene cassette in these integrons.
The endemic clonal dissemination and multi-drug resistance of CRPA isolates in our institution is highly alarming. Strict measure will be required to control the further spread of these pathogens in hospital setting.</description><identifier>ISSN: 2047-2994</identifier><identifier>EISSN: 2047-2994</identifier><identifier>DOI: 10.1186/s13756-015-0067-2</identifier><identifier>PMID: 26075066</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Analysis ; Antibacterial agents ; Disease transmission ; Drug resistance in microorganisms ; Epidemiology ; France ; Genetic aspects ; Health aspects ; Hospitals ; Prevalence studies (Epidemiology) ; Pseudomonas aeruginosa ; Risk factors</subject><ispartof>Antimicrobial resistance & infection control, 2015-06, Vol.4 (1), p.27-27, Article 27</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Meradji et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-c7031b0fcb727d1c27df8e66529beb1cea169cfc3d5076363c3c84f36f8871d3</citedby><cites>FETCH-LOGICAL-c497t-c7031b0fcb727d1c27df8e66529beb1cea169cfc3d5076363c3c84f36f8871d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4465145/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4465145/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26075066$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meradji, Samah</creatorcontrib><creatorcontrib>Barguigua, Abouddihaj</creatorcontrib><creatorcontrib>Zerouali, Khalid</creatorcontrib><creatorcontrib>Mazouz, Dekhil</creatorcontrib><creatorcontrib>Chettibi, Houria</creatorcontrib><creatorcontrib>Elmdaghri, Naima</creatorcontrib><creatorcontrib>Timinouni, Mohammed</creatorcontrib><title>Epidemiology of carbapenem non-susceptible Pseudomonas aeruginosa isolates in Eastern Algeria</title><title>Antimicrobial resistance & infection control</title><addtitle>Antimicrob Resist Infect Control</addtitle><description>Carbapenem resistance among Pseudomonas aeruginosa has become a serious life-threatening problem due to the limited therapeutic options. In this study, we investigated the prevalence and the molecular epidemiology of carbapenem resistant Pseudomonas aeruginosa (CRPA) isolated from three hospitals in Annaba city, Algeria.
During the study period (January, 2012 to December, 2013), all patients infected by P. aeruginosa were considered as the potential study population. Antibiotic susceptibility testing was performed as recommended by the CLSI. Screening of carbapenemase producer isolates was performed by using imipenem-EDTA double-disk synergy test and modified Hodge test. CRPA isolates were tested for the presence of genes encoding β-lactamases, plasmid mediated quinolone resistance, aminoglycoside resistance and class 1 integrons were investigated by PCR and sequencing. The clonal relatedness among CRPA isolates was analyzed by pulsed-field gel electrophoresis method. The clinical data were collected to identify risk factors for CRPA carriage of P. aeruginosa infection.
The overall prevalence of CRPA was 18.75 %. The risk factors for carrying CRPA were the length of hospital stay (p = 0.04), co-infections with Staphylococcus aureus (p = 0.01), and the use of urinary catheter (p = 0.03). The in-hospital mortality rate among case patients was 13.33 % compared with 1.53 % for control patients (p = 0.09). All CRPA isolates were multidrug resistance and the most effective antibiotic against CRPA isolates was amikacin and colistin. PFGE revealed an epidemic clonal dissemination of CRPA isolates. None of CRPA isolated were found to be carbapenemase-producers. The bla PSE-1 and aac(3)-II gene was detected in two and five strains respectively. The class1 integrons were detected in 2 isolates with the presence of aadA7 gene cassette in these integrons.
The endemic clonal dissemination and multi-drug resistance of CRPA isolates in our institution is highly alarming. Strict measure will be required to control the further spread of these pathogens in hospital setting.</description><subject>Analysis</subject><subject>Antibacterial agents</subject><subject>Disease transmission</subject><subject>Drug resistance in microorganisms</subject><subject>Epidemiology</subject><subject>France</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Hospitals</subject><subject>Prevalence studies (Epidemiology)</subject><subject>Pseudomonas aeruginosa</subject><subject>Risk factors</subject><issn>2047-2994</issn><issn>2047-2994</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNptkk9r3DAQxU1paUKaD9BLERRKL041li3Zl8IStn8g0B5yLUKWR14VWXIlO5BvX5lNwy4UgTRIv_dgRq8o3gK9AWj5pwRMNLyk0JSUclFWL4rLita56Lr65Ul9UVyn9JvSjaK0Za-Li4pT0VDOL4tf-9kOONngwvhIgiFaxV7N6HEiPvgyrUnjvNjeIfmZcB3CFLxKRGFcR-tDUsSm4NSCiVhP9iotGD3ZuRGjVW-KV0a5hNdP51Vx_2V_f_utvPvx9fvt7q7UdSeWUgvKoKdG96ISA-i8mRY5b6quxx40KuCdNpoNDRWccaaZbmvDuGlbAQO7Kj4fbee1n3DQ6JeonJyjnVR8lEFZef7i7UGO4UHWNW-gbrLBxyeDGP6smBY52dy3c8pjWJME3nYMABjL6PsjOiqH0noTsqPecLlrauCcthwydfMfKq9t1jp4NDbfnwk-nAgOqNxyyHNdFxt8OgfhCOoYUopontsEKrdgyGMwZA6G3L5cVlnz7nQ-z4p_MWB_AaNis8w</recordid><startdate>20150612</startdate><enddate>20150612</enddate><creator>Meradji, Samah</creator><creator>Barguigua, Abouddihaj</creator><creator>Zerouali, Khalid</creator><creator>Mazouz, Dekhil</creator><creator>Chettibi, Houria</creator><creator>Elmdaghri, Naima</creator><creator>Timinouni, Mohammed</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150612</creationdate><title>Epidemiology of carbapenem non-susceptible Pseudomonas aeruginosa isolates in Eastern Algeria</title><author>Meradji, Samah ; Barguigua, Abouddihaj ; Zerouali, Khalid ; Mazouz, Dekhil ; Chettibi, Houria ; Elmdaghri, Naima ; Timinouni, Mohammed</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-c7031b0fcb727d1c27df8e66529beb1cea169cfc3d5076363c3c84f36f8871d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Antibacterial agents</topic><topic>Disease transmission</topic><topic>Drug resistance in microorganisms</topic><topic>Epidemiology</topic><topic>France</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Hospitals</topic><topic>Prevalence studies (Epidemiology)</topic><topic>Pseudomonas aeruginosa</topic><topic>Risk factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meradji, Samah</creatorcontrib><creatorcontrib>Barguigua, Abouddihaj</creatorcontrib><creatorcontrib>Zerouali, Khalid</creatorcontrib><creatorcontrib>Mazouz, Dekhil</creatorcontrib><creatorcontrib>Chettibi, Houria</creatorcontrib><creatorcontrib>Elmdaghri, Naima</creatorcontrib><creatorcontrib>Timinouni, Mohammed</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Antimicrobial resistance & infection control</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meradji, Samah</au><au>Barguigua, Abouddihaj</au><au>Zerouali, Khalid</au><au>Mazouz, Dekhil</au><au>Chettibi, Houria</au><au>Elmdaghri, Naima</au><au>Timinouni, Mohammed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epidemiology of carbapenem non-susceptible Pseudomonas aeruginosa isolates in Eastern Algeria</atitle><jtitle>Antimicrobial resistance & infection control</jtitle><addtitle>Antimicrob Resist Infect Control</addtitle><date>2015-06-12</date><risdate>2015</risdate><volume>4</volume><issue>1</issue><spage>27</spage><epage>27</epage><pages>27-27</pages><artnum>27</artnum><issn>2047-2994</issn><eissn>2047-2994</eissn><abstract>Carbapenem resistance among Pseudomonas aeruginosa has become a serious life-threatening problem due to the limited therapeutic options. In this study, we investigated the prevalence and the molecular epidemiology of carbapenem resistant Pseudomonas aeruginosa (CRPA) isolated from three hospitals in Annaba city, Algeria.
During the study period (January, 2012 to December, 2013), all patients infected by P. aeruginosa were considered as the potential study population. Antibiotic susceptibility testing was performed as recommended by the CLSI. Screening of carbapenemase producer isolates was performed by using imipenem-EDTA double-disk synergy test and modified Hodge test. CRPA isolates were tested for the presence of genes encoding β-lactamases, plasmid mediated quinolone resistance, aminoglycoside resistance and class 1 integrons were investigated by PCR and sequencing. The clonal relatedness among CRPA isolates was analyzed by pulsed-field gel electrophoresis method. The clinical data were collected to identify risk factors for CRPA carriage of P. aeruginosa infection.
The overall prevalence of CRPA was 18.75 %. The risk factors for carrying CRPA were the length of hospital stay (p = 0.04), co-infections with Staphylococcus aureus (p = 0.01), and the use of urinary catheter (p = 0.03). The in-hospital mortality rate among case patients was 13.33 % compared with 1.53 % for control patients (p = 0.09). All CRPA isolates were multidrug resistance and the most effective antibiotic against CRPA isolates was amikacin and colistin. PFGE revealed an epidemic clonal dissemination of CRPA isolates. None of CRPA isolated were found to be carbapenemase-producers. The bla PSE-1 and aac(3)-II gene was detected in two and five strains respectively. The class1 integrons were detected in 2 isolates with the presence of aadA7 gene cassette in these integrons.
The endemic clonal dissemination and multi-drug resistance of CRPA isolates in our institution is highly alarming. Strict measure will be required to control the further spread of these pathogens in hospital setting.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26075066</pmid><doi>10.1186/s13756-015-0067-2</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Springer Nature OA Free Journals; Springer Nature - Complete Springer Journals; PubMed Central |
| subjects | Analysis Antibacterial agents Disease transmission Drug resistance in microorganisms Epidemiology France Genetic aspects Health aspects Hospitals Prevalence studies (Epidemiology) Pseudomonas aeruginosa Risk factors |
| title | Epidemiology of carbapenem non-susceptible Pseudomonas aeruginosa isolates in Eastern Algeria |
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