The significance of membrane fluidity of feeder cell-derived substrates for maintenance of iPS cell stemness

The biological activity of cell-derived substrates to maintain undifferentiated murine-induced pluripotent stem (iPS) cells was correlated to membrane fluidity as a new parameter of cell culture substrates. Murine embryonic fibroblasts (MEFs) were employed as feeder cells and their membrane fluidity...

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Veröffentlicht in:	Scientific reports 2015-06, Vol.5 (1), p.11386-11386, Article 11386
Hauptverfasser:	Zhou, Yue, Mao, Hongli, Joddar, Binata, Umeki, Nobuhisa, Sako, Yasushi, Wada, Ken-Ichi, Nishioka, Chieko, Takahashi, Eiki, Wang, Yi, Ito, Yoshihiro
Format:	Artikel
Sprache:	eng
Schlagworte:	13 13/100 13/106 14 14/63 631/532/2139 631/61/54/2295 Alkaline phosphatase Animals Biological activity Cell culture Cell Culture Techniques - methods Contamination Embryo fibroblasts Embryos Epidermal growth factor Epidermal growth factor receptors Feeder Cells - cytology Feeder Cells - metabolism Fluidity Formaldehyde Green fluorescent protein Growth factor receptors Humanities and Social Sciences Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Irradiation Membrane Fluidity Membranes Mice Mitomycin C multidisciplinary Pluripotency Science Stem cells Substrates
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