Phylogenetic Characterization of Phosphatase-Expressing Bacterial Communities in Baltic Sea Sediments
Phosphate release from sediments hampers the remediation of aquatic systems from a eutrophic state. Microbial phosphatases in sediments release phosphorus during organic matter degradation. Despite the important role of phosphatase-expressing bacteria, the identity of these bacteria in sediments is...
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| Veröffentlicht in: | Microbes and Environments 2015, Vol.30(2), pp.192-195 |
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| container_title | Microbes and Environments |
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| creator | Steenbergh, Anne K. Bodelier, Paul L.E. Hoogveld, Hans L. Slomp, Caroline P. Laanbroek, Hendrikus J. |
| description | Phosphate release from sediments hampers the remediation of aquatic systems from a eutrophic state. Microbial phosphatases in sediments release phosphorus during organic matter degradation. Despite the important role of phosphatase-expressing bacteria, the identity of these bacteria in sediments is largely unknown. We herein presented a culture-independent method to phylogenetically characterize phosphatase-expressing bacteria in sediments. We labeled whole-cell extracts of Baltic Sea sediments with an artificial phosphatase substrate and sorted phosphatase-expressing cells with a flow cytometer. Their phylogenetic affiliation was determined by Denaturing Gradient Gel Electrophoresis. The phosphatase-expressing bacterial community coarsely reflected the whole-cell bacterial community, with a similar dominance of Alphaproteobacteria. |
| doi_str_mv | 10.1264/jsme2.ME14074 |
| format | Article |
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Microbial phosphatases in sediments release phosphorus during organic matter degradation. Despite the important role of phosphatase-expressing bacteria, the identity of these bacteria in sediments is largely unknown. We herein presented a culture-independent method to phylogenetically characterize phosphatase-expressing bacteria in sediments. We labeled whole-cell extracts of Baltic Sea sediments with an artificial phosphatase substrate and sorted phosphatase-expressing cells with a flow cytometer. Their phylogenetic affiliation was determined by Denaturing Gradient Gel Electrophoresis. The phosphatase-expressing bacterial community coarsely reflected the whole-cell bacterial community, with a similar dominance of Alphaproteobacteria.</description><identifier>ISSN: 1342-6311</identifier><identifier>EISSN: 1347-4405</identifier><identifier>DOI: 10.1264/jsme2.ME14074</identifier><identifier>PMID: 25817584</identifier><language>eng</language><publisher>Japan: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles</publisher><subject>Bacteria ; Bacteria - classification ; Bacteria - enzymology ; Bacteria - genetics ; Biodiversity ; ELF labeling ; eutrophication ; flow cytometer ; Flow Cytometry ; Freshwater ; Geologic Sediments - microbiology ; Oceans and Seas ; phosphatase ; Phosphoric Monoester Hydrolases - metabolism ; phosphorus biogeochemistry ; Phylogeny ; Short Communication</subject><ispartof>Microbes and Environments, 2015, Vol.30(2), pp.192-195</ispartof><rights>2015 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions</rights><rights>Copyright Japan Science and Technology Agency 2015</rights><rights>Copyright 2015 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions 2015</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c658t-8ab88a046254a045960c77ce3e2142f47badc03f62885d202df388d66496a4c23</citedby><cites>FETCH-LOGICAL-c658t-8ab88a046254a045960c77ce3e2142f47badc03f62885d202df388d66496a4c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462931/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462931/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25817584$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Steenbergh, Anne K.</creatorcontrib><creatorcontrib>Bodelier, Paul L.E.</creatorcontrib><creatorcontrib>Hoogveld, Hans L.</creatorcontrib><creatorcontrib>Slomp, Caroline P.</creatorcontrib><creatorcontrib>Laanbroek, Hendrikus J.</creatorcontrib><title>Phylogenetic Characterization of Phosphatase-Expressing Bacterial Communities in Baltic Sea Sediments</title><title>Microbes and Environments</title><addtitle>Microbes Environ.</addtitle><description>Phosphate release from sediments hampers the remediation of aquatic systems from a eutrophic state. Microbial phosphatases in sediments release phosphorus during organic matter degradation. Despite the important role of phosphatase-expressing bacteria, the identity of these bacteria in sediments is largely unknown. We herein presented a culture-independent method to phylogenetically characterize phosphatase-expressing bacteria in sediments. We labeled whole-cell extracts of Baltic Sea sediments with an artificial phosphatase substrate and sorted phosphatase-expressing cells with a flow cytometer. Their phylogenetic affiliation was determined by Denaturing Gradient Gel Electrophoresis. The phosphatase-expressing bacterial community coarsely reflected the whole-cell bacterial community, with a similar dominance of Alphaproteobacteria.</description><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - enzymology</subject><subject>Bacteria - genetics</subject><subject>Biodiversity</subject><subject>ELF labeling</subject><subject>eutrophication</subject><subject>flow cytometer</subject><subject>Flow Cytometry</subject><subject>Freshwater</subject><subject>Geologic Sediments - microbiology</subject><subject>Oceans and Seas</subject><subject>phosphatase</subject><subject>Phosphoric Monoester Hydrolases - metabolism</subject><subject>phosphorus biogeochemistry</subject><subject>Phylogeny</subject><subject>Short Communication</subject><issn>1342-6311</issn><issn>1347-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1v1DAQxSNERUvhyBVF4sIlxd92Lkg0Wj6kVq1UOFteZ7LxKrEXO0GUvx7vblgBB3ssvZ_ezPgVxSuMrjAR7N02jUCubleYIcmeFBeYMlkxhvjTw5tUgmJ8XjxPaYsQpVySZ8U54QpLrthFAff94xA24GFytmx6E42dILpfZnLBl6Er7_uQdr2ZTIJq9XMXISXnN-X1kTND2YRxnL2bHKTS-SwMe6sHMPm0bgQ_pRfFWWeGBC-Xell8-7j62nyubu4-fWk-3FRWcDVVyqyVMogJwlkuvBbISmmBAsGMdEyuTWsR7QRRircEkbajSrVCsFoYZgm9LN4ffXfzeoTW5t7RDHoX3Wjiow7G6X8V73q9CT80yz1rirPB28Ughu8zpEmPLlkYBuMhzEljKXjNiKxZRt_8h27DHH1eL1NcEKIo3lPVkbIxpBShOw2Dkd4HqA8B6iXAzL_-e4MT_SexDDRHYJsms4ETYGL-9QEWO4r04VpsT6rNAWvw9DfuiLCD</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Steenbergh, Anne K.</creator><creator>Bodelier, Paul L.E.</creator><creator>Hoogveld, Hans L.</creator><creator>Slomp, Caroline P.</creator><creator>Laanbroek, Hendrikus J.</creator><general>Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles</general><general>Japan Science and Technology Agency</general><general>the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>7TN</scope><scope>7TV</scope><scope>5PM</scope></search><sort><creationdate>2015</creationdate><title>Phylogenetic Characterization of Phosphatase-Expressing Bacterial Communities in Baltic Sea Sediments</title><author>Steenbergh, Anne K. ; 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| subjects | Bacteria Bacteria - classification Bacteria - enzymology Bacteria - genetics Biodiversity ELF labeling eutrophication flow cytometer Flow Cytometry Freshwater Geologic Sediments - microbiology Oceans and Seas phosphatase Phosphoric Monoester Hydrolases - metabolism phosphorus biogeochemistry Phylogeny Short Communication |
| title | Phylogenetic Characterization of Phosphatase-Expressing Bacterial Communities in Baltic Sea Sediments |
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