TRANSPARENT TESTA GLABRA1 and GLABRA1 Compete for Binding to GLABRA3 in Arabidopsis
The MBW (for R2R3MYB, basic helix-loop-helix [bHLH], and WD40) genes comprise an evolutionarily conserved gene cassette that regulates several traits such as (pro)anthocyanin and anthocyanin biosynthesis and epidermal cell differentiation in plants. Trichome differentiation in Arabidopsis (Arabidops...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2015-06, Vol.168 (2), p.584-597 |
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| creator | Pesch, Martina Schultheiß, Ilka Klopffleisch, Karsten Uhrig, Joachim F. Koegl, Manfred Clemen, Christoph S. Simon, Rüdiger Weidtkamp-Peters, Stefanie Hülskamp, Martin |
| description | The MBW (for R2R3MYB, basic helix-loop-helix [bHLH], and WD40) genes comprise an evolutionarily conserved gene cassette that regulates several traits such as (pro)anthocyanin and anthocyanin biosynthesis and epidermal cell differentiation in plants. Trichome differentiation in Arabidopsis (Arabidopsis thaliana) is governed by GLABRA1 (GL1; R2R3MYB), GL3 (bHLH), andTRANSPARENT TESTA GLABRA1(TTG1; WD40). They are thought to form a trimeric complex that acts as a transcriptional activation complex. We provide evidence that these three MBW proteins form either GL1 GL3 or GL3 TTG1 dimers. The formation of each dimer is counteracted by the respective third protein in yeast three-hybrid assays, pulldown experiments (luminescence-based mammalian interactome), and fluorescence lifetime imaging microscopy-fluorescence resonance energy transfer studies. We further show that two target promoters,TRIPTYCHON(TRY) andCAPRICE(CPC), are differentially regulated: GL1 represses the activation of theTRYpromoter by GL3 and TTG1, and TTG1 suppresses the activation of theCPCpromoter by GL1 and GL3. Our data suggest that the transcriptional activation by the MBW complex involves alternative complex formation and that the two dimers can differentially regulate downstream genes. |
| doi_str_mv | 10.1104/pp.15.00328 |
| format | Article |
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Trichome differentiation in Arabidopsis (Arabidopsis thaliana) is governed by GLABRA1 (GL1; R2R3MYB), GL3 (bHLH), andTRANSPARENT TESTA GLABRA1(TTG1; WD40). They are thought to form a trimeric complex that acts as a transcriptional activation complex. We provide evidence that these three MBW proteins form either GL1 GL3 or GL3 TTG1 dimers. The formation of each dimer is counteracted by the respective third protein in yeast three-hybrid assays, pulldown experiments (luminescence-based mammalian interactome), and fluorescence lifetime imaging microscopy-fluorescence resonance energy transfer studies. We further show that two target promoters,TRIPTYCHON(TRY) andCAPRICE(CPC), are differentially regulated: GL1 represses the activation of theTRYpromoter by GL3 and TTG1, and TTG1 suppresses the activation of theCPCpromoter by GL1 and GL3. Our data suggest that the transcriptional activation by the MBW complex involves alternative complex formation and that the two dimers can differentially regulate downstream genes.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.15.00328</identifier><identifier>PMID: 25926482</identifier><language>eng</language><publisher>United States: American Society of Plant Biologists</publisher><subject>American culture ; Arabidopsis ; Arabidopsis - cytology ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Basic Helix-Loop-Helix Transcription Factors - metabolism ; Binding, Competitive ; Biology ; DNA-Binding Proteins - metabolism ; Epidermal cells ; Fluorescence ; Fluorescence Resonance Energy Transfer ; Gene expression regulation ; Gene Expression Regulation, Plant ; genes ; GENES, DEVELOPMENT, AND EVOLUTION ; Microscopy, Fluorescence ; Models, Biological ; Plants ; Plants, Genetically Modified ; Promoter Regions, Genetic - genetics ; Protein Binding ; Protein Interaction Mapping ; Proteins ; Proto-Oncogene Proteins c-myb - genetics ; Proto-Oncogene Proteins c-myb - metabolism ; Root hairs ; testa ; transactivators ; Transformation, Genetic ; Trichomes ; Two-Hybrid System Techniques ; Yeasts</subject><ispartof>Plant physiology (Bethesda), 2015-06, Vol.168 (2), p.584-597</ispartof><rights>Copyright © 2015 American Society of Plant Biologists</rights><rights>2015 American Society of Plant Biologists. All Rights Reserved.</rights><rights>2015 American Society of Plant Biologists. All Rights Reserved. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-42480503e5ed84fdea1d8779dd23a828c319f133c564f253ddbaf2f1a80f36f83</citedby><orcidid>0000-0002-1317-7716 ; 0000-0001-7734-3771 ; 0000-0001-6633-9769 ; 0000-0002-6592-4445</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/24805411$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/24805411$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,777,781,800,882,27905,27906,57998,58231</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25926482$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pesch, Martina</creatorcontrib><creatorcontrib>Schultheiß, Ilka</creatorcontrib><creatorcontrib>Klopffleisch, Karsten</creatorcontrib><creatorcontrib>Uhrig, Joachim F.</creatorcontrib><creatorcontrib>Koegl, Manfred</creatorcontrib><creatorcontrib>Clemen, Christoph S.</creatorcontrib><creatorcontrib>Simon, Rüdiger</creatorcontrib><creatorcontrib>Weidtkamp-Peters, Stefanie</creatorcontrib><creatorcontrib>Hülskamp, Martin</creatorcontrib><title>TRANSPARENT TESTA GLABRA1 and GLABRA1 Compete for Binding to GLABRA3 in Arabidopsis</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The MBW (for R2R3MYB, basic helix-loop-helix [bHLH], and WD40) genes comprise an evolutionarily conserved gene cassette that regulates several traits such as (pro)anthocyanin and anthocyanin biosynthesis and epidermal cell differentiation in plants. Trichome differentiation in Arabidopsis (Arabidopsis thaliana) is governed by GLABRA1 (GL1; R2R3MYB), GL3 (bHLH), andTRANSPARENT TESTA GLABRA1(TTG1; WD40). They are thought to form a trimeric complex that acts as a transcriptional activation complex. We provide evidence that these three MBW proteins form either GL1 GL3 or GL3 TTG1 dimers. The formation of each dimer is counteracted by the respective third protein in yeast three-hybrid assays, pulldown experiments (luminescence-based mammalian interactome), and fluorescence lifetime imaging microscopy-fluorescence resonance energy transfer studies. We further show that two target promoters,TRIPTYCHON(TRY) andCAPRICE(CPC), are differentially regulated: GL1 represses the activation of theTRYpromoter by GL3 and TTG1, and TTG1 suppresses the activation of theCPCpromoter by GL1 and GL3. Our data suggest that the transcriptional activation by the MBW complex involves alternative complex formation and that the two dimers can differentially regulate downstream genes.</description><subject>American culture</subject><subject>Arabidopsis</subject><subject>Arabidopsis - cytology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Basic Helix-Loop-Helix Transcription Factors - metabolism</subject><subject>Binding, Competitive</subject><subject>Biology</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Epidermal cells</subject><subject>Fluorescence</subject><subject>Fluorescence Resonance Energy Transfer</subject><subject>Gene expression regulation</subject><subject>Gene Expression Regulation, Plant</subject><subject>genes</subject><subject>GENES, DEVELOPMENT, AND EVOLUTION</subject><subject>Microscopy, Fluorescence</subject><subject>Models, Biological</subject><subject>Plants</subject><subject>Plants, Genetically Modified</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein Binding</subject><subject>Protein Interaction Mapping</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-myb - genetics</subject><subject>Proto-Oncogene Proteins c-myb - metabolism</subject><subject>Root hairs</subject><subject>testa</subject><subject>transactivators</subject><subject>Transformation, Genetic</subject><subject>Trichomes</subject><subject>Two-Hybrid System Techniques</subject><subject>Yeasts</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctLAzEQxoMoWh8nz8oeBdmayWObvQhrqQ8oKu16Dukm0Ui7WZOt4H9va2vVk6cZ5vvxMTMfQseAuwCYXTRNF3gXY0rEFuoApyQlnIlt1FnOUixEvof2Y3zFGAMFtov2CM9JxgTpoHE5Ku7Hj8VocF8m5WBcFsnNsLgaFZCoWm_6vp81pjWJ9SG5crV29XPS-rVME1cnRVATp30TXTxEO1ZNozla1wP0dD0o-7fp8OHmrl8M04rRrE0ZYQJzTA03WjCrjQIter1ca0KVIKKikFugtOIZs4RTrSfKEgtKYEszK-gBulz5NvPJzOjK1G1QU9kEN1PhQ3rl5F-ldi_y2b9Lxjjt5XhhcLY2CP5tbmIrZy5WZjpVtfHzKMnyY0Ao-x-FTPBehgFnC_R8hVbBxxiM3WwEWC4Tk00jgcuvxBb06e8jNux3RAvgZAW8xtaHH335PAZAPwH2vJZp</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Pesch, Martina</creator><creator>Schultheiß, Ilka</creator><creator>Klopffleisch, Karsten</creator><creator>Uhrig, Joachim F.</creator><creator>Koegl, Manfred</creator><creator>Clemen, Christoph S.</creator><creator>Simon, Rüdiger</creator><creator>Weidtkamp-Peters, Stefanie</creator><creator>Hülskamp, Martin</creator><general>American Society of Plant Biologists</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1317-7716</orcidid><orcidid>https://orcid.org/0000-0001-7734-3771</orcidid><orcidid>https://orcid.org/0000-0001-6633-9769</orcidid><orcidid>https://orcid.org/0000-0002-6592-4445</orcidid></search><sort><creationdate>20150601</creationdate><title>TRANSPARENT TESTA GLABRA1 and GLABRA1 Compete for Binding to GLABRA3 in Arabidopsis</title><author>Pesch, Martina ; Schultheiß, Ilka ; Klopffleisch, Karsten ; Uhrig, Joachim F. ; Koegl, Manfred ; Clemen, Christoph S. ; Simon, Rüdiger ; Weidtkamp-Peters, Stefanie ; Hülskamp, Martin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-42480503e5ed84fdea1d8779dd23a828c319f133c564f253ddbaf2f1a80f36f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>American culture</topic><topic>Arabidopsis</topic><topic>Arabidopsis - cytology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Basic Helix-Loop-Helix Transcription Factors - metabolism</topic><topic>Binding, Competitive</topic><topic>Biology</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Epidermal cells</topic><topic>Fluorescence</topic><topic>Fluorescence Resonance Energy Transfer</topic><topic>Gene expression regulation</topic><topic>Gene Expression Regulation, Plant</topic><topic>genes</topic><topic>GENES, DEVELOPMENT, AND EVOLUTION</topic><topic>Microscopy, Fluorescence</topic><topic>Models, Biological</topic><topic>Plants</topic><topic>Plants, Genetically Modified</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protein Binding</topic><topic>Protein Interaction Mapping</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins c-myb - genetics</topic><topic>Proto-Oncogene Proteins c-myb - metabolism</topic><topic>Root hairs</topic><topic>testa</topic><topic>transactivators</topic><topic>Transformation, Genetic</topic><topic>Trichomes</topic><topic>Two-Hybrid System Techniques</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pesch, Martina</creatorcontrib><creatorcontrib>Schultheiß, Ilka</creatorcontrib><creatorcontrib>Klopffleisch, Karsten</creatorcontrib><creatorcontrib>Uhrig, Joachim F.</creatorcontrib><creatorcontrib>Koegl, Manfred</creatorcontrib><creatorcontrib>Clemen, Christoph S.</creatorcontrib><creatorcontrib>Simon, Rüdiger</creatorcontrib><creatorcontrib>Weidtkamp-Peters, Stefanie</creatorcontrib><creatorcontrib>Hülskamp, Martin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pesch, Martina</au><au>Schultheiß, Ilka</au><au>Klopffleisch, Karsten</au><au>Uhrig, Joachim F.</au><au>Koegl, Manfred</au><au>Clemen, Christoph S.</au><au>Simon, Rüdiger</au><au>Weidtkamp-Peters, Stefanie</au><au>Hülskamp, Martin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TRANSPARENT TESTA GLABRA1 and GLABRA1 Compete for Binding to GLABRA3 in Arabidopsis</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>168</volume><issue>2</issue><spage>584</spage><epage>597</epage><pages>584-597</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>The MBW (for R2R3MYB, basic helix-loop-helix [bHLH], and WD40) genes comprise an evolutionarily conserved gene cassette that regulates several traits such as (pro)anthocyanin and anthocyanin biosynthesis and epidermal cell differentiation in plants. Trichome differentiation in Arabidopsis (Arabidopsis thaliana) is governed by GLABRA1 (GL1; R2R3MYB), GL3 (bHLH), andTRANSPARENT TESTA GLABRA1(TTG1; WD40). They are thought to form a trimeric complex that acts as a transcriptional activation complex. We provide evidence that these three MBW proteins form either GL1 GL3 or GL3 TTG1 dimers. The formation of each dimer is counteracted by the respective third protein in yeast three-hybrid assays, pulldown experiments (luminescence-based mammalian interactome), and fluorescence lifetime imaging microscopy-fluorescence resonance energy transfer studies. We further show that two target promoters,TRIPTYCHON(TRY) andCAPRICE(CPC), are differentially regulated: GL1 represses the activation of theTRYpromoter by GL3 and TTG1, and TTG1 suppresses the activation of theCPCpromoter by GL1 and GL3. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current) |
| subjects | American culture Arabidopsis Arabidopsis - cytology Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Basic Helix-Loop-Helix Transcription Factors - metabolism Binding, Competitive Biology DNA-Binding Proteins - metabolism Epidermal cells Fluorescence Fluorescence Resonance Energy Transfer Gene expression regulation Gene Expression Regulation, Plant genes GENES, DEVELOPMENT, AND EVOLUTION Microscopy, Fluorescence Models, Biological Plants Plants, Genetically Modified Promoter Regions, Genetic - genetics Protein Binding Protein Interaction Mapping Proteins Proto-Oncogene Proteins c-myb - genetics Proto-Oncogene Proteins c-myb - metabolism Root hairs testa transactivators Transformation, Genetic Trichomes Two-Hybrid System Techniques Yeasts |
| title | TRANSPARENT TESTA GLABRA1 and GLABRA1 Compete for Binding to GLABRA3 in Arabidopsis |
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