Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats
Transplantation of endothelial progenitor cells (EPCs) restores endothelial function in patients with endothelial dysfunction and initial denudation. The goal of the present study was to determine the effect of cryopreserved human umbilical cord blood (UCB)-derived EPC infusion on the repair of caro...
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| Veröffentlicht in: | Stem cell research & therapy 2015-04, Vol.6 (1), p.37-37, Article 37 |
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| creator | Yin, Yangguang Liu, Huanyun Wang, Fangjuan Li, Lufeng Deng, Mengyang Huang, Lan Zhao, Xiaohui |
| description | Transplantation of endothelial progenitor cells (EPCs) restores endothelial function in patients with endothelial dysfunction and initial denudation. The goal of the present study was to determine the effect of cryopreserved human umbilical cord blood (UCB)-derived EPC infusion on the repair of carotid artery injury in nude rats.
Mononuclear cells (MNCs) from human cryopreserved UCB and peripheral blood (PB) of patients with cardiovascular diseases and healthy volunteers were cultured in a conditioned medium. The in vitro migration, proliferation, adhesion, and survival capacities, as well as paracrine cytokine release of EPCs were investigated. EPC homing, induced reendothelialization, and the effect on neointima formation were also assessed in vivo.
Patient-derived PB EPCs (PPB-EPCs) displayed decreased migration, proliferation, adhesion, and survival capabilities as compared to PB-EPCs from healthy volunteers (HPB-EPCs) and cryopreserved UCB-EPCs. However, there was no difference in the release of vascular endothelial growth factor (VEGF) and stromal cell derived factor 1 (SDF-1) between the three groups. Two weeks after transplantation, more labeled UCB-EPCs and HPB-EPCs than PPB-EPCs were found by cell tracking in the injury zone. Administration of PPB-EPCs, HPB-EPCs, and UCB-EPCs enhanced reendothelialization and inhibited neointima formation compared to the saline control. However, UCB-EPC and HPB-EPC infusion showed a greater improvement than PPB-EPCs.
Cryopreserved UCB-MNCs derived EPCs and HPB-EPCs show better responses to cytokines and vascular injury than PPB-EPCs. Thus, cryopreservation and delivery of cryopreserved autogenous UCB-EPCs or HPB-EPCs may be a promising vasculoprotective approach for patients with multiple cardiovascular risk factors. |
| doi_str_mv | 10.1186/s13287-015-0022-4 |
| format | Article |
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Mononuclear cells (MNCs) from human cryopreserved UCB and peripheral blood (PB) of patients with cardiovascular diseases and healthy volunteers were cultured in a conditioned medium. The in vitro migration, proliferation, adhesion, and survival capacities, as well as paracrine cytokine release of EPCs were investigated. EPC homing, induced reendothelialization, and the effect on neointima formation were also assessed in vivo.
Patient-derived PB EPCs (PPB-EPCs) displayed decreased migration, proliferation, adhesion, and survival capabilities as compared to PB-EPCs from healthy volunteers (HPB-EPCs) and cryopreserved UCB-EPCs. However, there was no difference in the release of vascular endothelial growth factor (VEGF) and stromal cell derived factor 1 (SDF-1) between the three groups. Two weeks after transplantation, more labeled UCB-EPCs and HPB-EPCs than PPB-EPCs were found by cell tracking in the injury zone. Administration of PPB-EPCs, HPB-EPCs, and UCB-EPCs enhanced reendothelialization and inhibited neointima formation compared to the saline control. However, UCB-EPC and HPB-EPC infusion showed a greater improvement than PPB-EPCs.
Cryopreserved UCB-MNCs derived EPCs and HPB-EPCs show better responses to cytokines and vascular injury than PPB-EPCs. Thus, cryopreservation and delivery of cryopreserved autogenous UCB-EPCs or HPB-EPCs may be a promising vasculoprotective approach for patients with multiple cardiovascular risk factors.</description><identifier>ISSN: 1757-6512</identifier><identifier>EISSN: 1757-6512</identifier><identifier>DOI: 10.1186/s13287-015-0022-4</identifier><identifier>PMID: 25956351</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Aged ; Animals ; Cardiovascular diseases ; Care and treatment ; Carotid Arteries - metabolism ; Carotid Arteries - pathology ; Carotid Artery Injuries - therapy ; Cell Adhesion ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Cryopreservation ; Cytokines ; Cytokines - metabolism ; Endothelial Progenitor Cells - cytology ; Endothelial Progenitor Cells - metabolism ; Endothelial Progenitor Cells - transplantation ; Endothelium ; Female ; Fetal Blood - cytology ; Health aspects ; Humans ; Leukocytes, Mononuclear - cytology ; Leukocytes, Mononuclear - metabolism ; Leukocytes, Mononuclear - transplantation ; Male ; Middle Aged ; Physiological aspects ; Rats ; Rats, Nude ; Transplantation, Heterologous ; Vascular endothelial growth factor</subject><ispartof>Stem cell research & therapy, 2015-04, Vol.6 (1), p.37-37, Article 37</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Yin et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c601t-151637d9b7e87db7d969a71016170d037b81fcc1d502cdcd62bca810f736ab8d3</citedby><cites>FETCH-LOGICAL-c601t-151637d9b7e87db7d969a71016170d037b81fcc1d502cdcd62bca810f736ab8d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4453210/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4453210/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25956351$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yin, Yangguang</creatorcontrib><creatorcontrib>Liu, Huanyun</creatorcontrib><creatorcontrib>Wang, Fangjuan</creatorcontrib><creatorcontrib>Li, Lufeng</creatorcontrib><creatorcontrib>Deng, Mengyang</creatorcontrib><creatorcontrib>Huang, Lan</creatorcontrib><creatorcontrib>Zhao, Xiaohui</creatorcontrib><title>Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats</title><title>Stem cell research & therapy</title><addtitle>Stem Cell Res Ther</addtitle><description>Transplantation of endothelial progenitor cells (EPCs) restores endothelial function in patients with endothelial dysfunction and initial denudation. The goal of the present study was to determine the effect of cryopreserved human umbilical cord blood (UCB)-derived EPC infusion on the repair of carotid artery injury in nude rats.
Mononuclear cells (MNCs) from human cryopreserved UCB and peripheral blood (PB) of patients with cardiovascular diseases and healthy volunteers were cultured in a conditioned medium. The in vitro migration, proliferation, adhesion, and survival capacities, as well as paracrine cytokine release of EPCs were investigated. EPC homing, induced reendothelialization, and the effect on neointima formation were also assessed in vivo.
Patient-derived PB EPCs (PPB-EPCs) displayed decreased migration, proliferation, adhesion, and survival capabilities as compared to PB-EPCs from healthy volunteers (HPB-EPCs) and cryopreserved UCB-EPCs. However, there was no difference in the release of vascular endothelial growth factor (VEGF) and stromal cell derived factor 1 (SDF-1) between the three groups. Two weeks after transplantation, more labeled UCB-EPCs and HPB-EPCs than PPB-EPCs were found by cell tracking in the injury zone. Administration of PPB-EPCs, HPB-EPCs, and UCB-EPCs enhanced reendothelialization and inhibited neointima formation compared to the saline control. However, UCB-EPC and HPB-EPC infusion showed a greater improvement than PPB-EPCs.
Cryopreserved UCB-MNCs derived EPCs and HPB-EPCs show better responses to cytokines and vascular injury than PPB-EPCs. Thus, cryopreservation and delivery of cryopreserved autogenous UCB-EPCs or HPB-EPCs may be a promising vasculoprotective approach for patients with multiple cardiovascular risk factors.</description><subject>Aged</subject><subject>Animals</subject><subject>Cardiovascular diseases</subject><subject>Care and treatment</subject><subject>Carotid Arteries - metabolism</subject><subject>Carotid Arteries - pathology</subject><subject>Carotid Artery Injuries - therapy</subject><subject>Cell Adhesion</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Cryopreservation</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Endothelial Progenitor Cells - cytology</subject><subject>Endothelial Progenitor Cells - metabolism</subject><subject>Endothelial Progenitor Cells - transplantation</subject><subject>Endothelium</subject><subject>Female</subject><subject>Fetal Blood - cytology</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Leukocytes, Mononuclear - transplantation</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Physiological aspects</subject><subject>Rats</subject><subject>Rats, Nude</subject><subject>Transplantation, Heterologous</subject><subject>Vascular endothelial growth factor</subject><issn>1757-6512</issn><issn>1757-6512</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkt9rFDEQxxdRbDn7B_giAUH0YWtmf2T3XoRSqhYKgtbnkE1mb1OyyZlkj95f4r_bbK-WWzB5mGHmMxMy882yt0DPAVr2OUBZtE1Ooc4pLYq8epGdQlM3OauheHnkn2RnIdzRdMqSUla9zk6Kel2zsobT7O-tFzZsjbBRRO0scT2Rfu-2HgP6HSoyTKOwZBo7bbQUhkjnFemMcypX6PWMoFUuDmh0Sm-926DV0Xki0ZhAtFWTxEA8SrdDv398QXgXtSLCxzmi7d30aIidFBIvYniTveqFCXj2ZFfZ769Xt5ff85sf364vL25yySjEHGpgZaPWXYNto7rksbVogAKDhipaNl0LvZSgalpIJRUrOilaoH1TMtG1qlxlXw59t1M3opJooxeGb70ehd9zJzRfZqwe-MbteFXVZQE0Nfj41MC7PxOGyEcd5p8Li24KHFjLKGNVmvcqe39AN8Ig17Z3qaOccX5RVzAvhEKizv9Dpatw1NJZ7HWKLwo-LQoSE_E-bsQUAr_-9XPJfjhiBxQmDsGZaV59WIJwAKV3IXjsn0cClM_64wf98aQ_PuuPV6nm3fEsnyv-qa18AFhP18g</recordid><startdate>20150417</startdate><enddate>20150417</enddate><creator>Yin, Yangguang</creator><creator>Liu, Huanyun</creator><creator>Wang, Fangjuan</creator><creator>Li, Lufeng</creator><creator>Deng, Mengyang</creator><creator>Huang, Lan</creator><creator>Zhao, Xiaohui</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150417</creationdate><title>Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats</title><author>Yin, Yangguang ; Liu, Huanyun ; Wang, Fangjuan ; Li, Lufeng ; Deng, Mengyang ; Huang, Lan ; Zhao, Xiaohui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c601t-151637d9b7e87db7d969a71016170d037b81fcc1d502cdcd62bca810f736ab8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aged</topic><topic>Animals</topic><topic>Cardiovascular diseases</topic><topic>Care and treatment</topic><topic>Carotid Arteries - metabolism</topic><topic>Carotid Arteries - pathology</topic><topic>Carotid Artery Injuries - therapy</topic><topic>Cell Adhesion</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Cryopreservation</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Endothelial Progenitor Cells - cytology</topic><topic>Endothelial Progenitor Cells - metabolism</topic><topic>Endothelial Progenitor Cells - transplantation</topic><topic>Endothelium</topic><topic>Female</topic><topic>Fetal Blood - cytology</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - cytology</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Leukocytes, Mononuclear - transplantation</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Physiological aspects</topic><topic>Rats</topic><topic>Rats, Nude</topic><topic>Transplantation, Heterologous</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yin, Yangguang</creatorcontrib><creatorcontrib>Liu, Huanyun</creatorcontrib><creatorcontrib>Wang, Fangjuan</creatorcontrib><creatorcontrib>Li, Lufeng</creatorcontrib><creatorcontrib>Deng, Mengyang</creatorcontrib><creatorcontrib>Huang, Lan</creatorcontrib><creatorcontrib>Zhao, Xiaohui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Stem cell research & therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Yangguang</au><au>Liu, Huanyun</au><au>Wang, Fangjuan</au><au>Li, Lufeng</au><au>Deng, Mengyang</au><au>Huang, Lan</au><au>Zhao, Xiaohui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats</atitle><jtitle>Stem cell research & therapy</jtitle><addtitle>Stem Cell Res Ther</addtitle><date>2015-04-17</date><risdate>2015</risdate><volume>6</volume><issue>1</issue><spage>37</spage><epage>37</epage><pages>37-37</pages><artnum>37</artnum><issn>1757-6512</issn><eissn>1757-6512</eissn><abstract>Transplantation of endothelial progenitor cells (EPCs) restores endothelial function in patients with endothelial dysfunction and initial denudation. The goal of the present study was to determine the effect of cryopreserved human umbilical cord blood (UCB)-derived EPC infusion on the repair of carotid artery injury in nude rats.
Mononuclear cells (MNCs) from human cryopreserved UCB and peripheral blood (PB) of patients with cardiovascular diseases and healthy volunteers were cultured in a conditioned medium. The in vitro migration, proliferation, adhesion, and survival capacities, as well as paracrine cytokine release of EPCs were investigated. EPC homing, induced reendothelialization, and the effect on neointima formation were also assessed in vivo.
Patient-derived PB EPCs (PPB-EPCs) displayed decreased migration, proliferation, adhesion, and survival capabilities as compared to PB-EPCs from healthy volunteers (HPB-EPCs) and cryopreserved UCB-EPCs. However, there was no difference in the release of vascular endothelial growth factor (VEGF) and stromal cell derived factor 1 (SDF-1) between the three groups. Two weeks after transplantation, more labeled UCB-EPCs and HPB-EPCs than PPB-EPCs were found by cell tracking in the injury zone. Administration of PPB-EPCs, HPB-EPCs, and UCB-EPCs enhanced reendothelialization and inhibited neointima formation compared to the saline control. However, UCB-EPC and HPB-EPC infusion showed a greater improvement than PPB-EPCs.
Cryopreserved UCB-MNCs derived EPCs and HPB-EPCs show better responses to cytokines and vascular injury than PPB-EPCs. Thus, cryopreservation and delivery of cryopreserved autogenous UCB-EPCs or HPB-EPCs may be a promising vasculoprotective approach for patients with multiple cardiovascular risk factors.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>25956351</pmid><doi>10.1186/s13287-015-0022-4</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Animals Cardiovascular diseases Care and treatment Carotid Arteries - metabolism Carotid Arteries - pathology Carotid Artery Injuries - therapy Cell Adhesion Cell Movement Cell Proliferation Cells, Cultured Cryopreservation Cytokines Cytokines - metabolism Endothelial Progenitor Cells - cytology Endothelial Progenitor Cells - metabolism Endothelial Progenitor Cells - transplantation Endothelium Female Fetal Blood - cytology Health aspects Humans Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - metabolism Leukocytes, Mononuclear - transplantation Male Middle Aged Physiological aspects Rats Rats, Nude Transplantation, Heterologous Vascular endothelial growth factor |
| title | Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats |
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