Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy
Objectives. We investigated the action of triptolide in rats with adriamycin-induced nephropathy and evaluated the possible mechanisms underlying its protective effect against podocyte injury. Methods. In total, 30 healthy male Sprague-Dawley rats were randomized into three groups (normal group, mod...
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| creator | Sun, Wei Li, Chun-Qing Zhu, Hao Tu, Yue Wei, Ming-Gang Jiang, Chun-Bo Jing, Wei-Min |
| description | Objectives. We investigated the action of triptolide in rats with adriamycin-induced nephropathy and evaluated the possible mechanisms underlying its protective effect against podocyte injury. Methods. In total, 30 healthy male Sprague-Dawley rats were randomized into three groups (normal group, model group, and triptolide group). On days 7, 28, 42, and 56, 24 h urine samples were collected. All rats were sacrificed on day 56, and their blood and renal tissues were collected for determination of biochemical and molecular biological parameters. Expression of miRNAs in the renal cortex was analyzed by a biochip assay and RT-PCR was used to confirm observed differences in miRNA levels. Results. Triptolide decreased proteinuria, improved renal function without apparent adverse effects on the liver, and alleviated renal pathological lesions. Triptolide also elevated the nephrin protein level. Furthermore, levels of miR-344b-3p and miR-30b-3p were elevated in rats with adriamycin-induced nephropathy, while triptolide treatment reversed the increase in the expression of these two miRNAs. Conclusions. These results suggest that triptolide may attenuate podocyte injury in rats with adriamycin-induced nephropathy by regulating expression of miRNA-344b-3p and miRNA-30b-3p. |
| doi_str_mv | 10.1155/2015/107814 |
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| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4452866</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A458645176</galeid><sourcerecordid>A458645176</sourcerecordid><originalsourceid>FETCH-LOGICAL-c495t-967bcd6e58cdb7ae25ca5f07ea1da2363a05200cd5c9e75f51e7d424535f686b3</originalsourceid><addsrcrecordid>eNqNkk9rFDEYhwdRbK2evEvAiyhjk0z-zFyEpVRdKFWWCt5CJnlnN2U2GZOMdb6DH9pZt12rJ08Jbx6eN2_yK4rnBL8lhPNTigk_JVjWhD0ojolkpGS0rh8e9vLrUfEkpWuMaSOlfFwcUTHjUojj4udVdEMOvbOAFjmDH3WGhD4HG8yUAS399Rgn1E5oBeux19n5NTr_MURIyQWPQoe2bnW5KCvG2rIakPb2roJ_F5xHK50TunF5gxY2Or2djPPl0tvRgEWXMGxiGHTeTE-LR53uEzy7XU-KL-_Pr84-lhefPizPFhelYQ3PZSNka6wAXhvbSg2UG807LEETq2klKo05xdhYbhqQvOMEpGWU8Yp3ohZtdVK823uHsd2CNeBz1L0aotvqOKmgnfr7xLuNWofvijFOayFmwatbQQzfRkhZbV0y0PfaQxiTIqJuBKlrSWb05T_odRijn8dTROKGNphI-oda6x6U812Y-5qdVC0YrwXjRO7avtlTJoaUInSHKxOsdllQuyyofRZm-sX9KQ_s3efPwOs9sHHe6hv3fzaYEej0PVgQMr_tL3_VxQ4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1709290172</pqid></control><display><type>article</type><title>Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy</title><source>PubMed Central Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library (Open Access Collection)</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Sun, Wei ; Li, Chun-Qing ; Zhu, Hao ; Tu, Yue ; Wei, Ming-Gang ; Jiang, Chun-Bo ; Jing, Wei-Min</creator><contributor>Schmeda-Hirschmann, Guillermo</contributor><creatorcontrib>Sun, Wei ; Li, Chun-Qing ; Zhu, Hao ; Tu, Yue ; Wei, Ming-Gang ; Jiang, Chun-Bo ; Jing, Wei-Min ; Schmeda-Hirschmann, Guillermo</creatorcontrib><description>Objectives. We investigated the action of triptolide in rats with adriamycin-induced nephropathy and evaluated the possible mechanisms underlying its protective effect against podocyte injury. Methods. In total, 30 healthy male Sprague-Dawley rats were randomized into three groups (normal group, model group, and triptolide group). On days 7, 28, 42, and 56, 24 h urine samples were collected. All rats were sacrificed on day 56, and their blood and renal tissues were collected for determination of biochemical and molecular biological parameters. Expression of miRNAs in the renal cortex was analyzed by a biochip assay and RT-PCR was used to confirm observed differences in miRNA levels. Results. Triptolide decreased proteinuria, improved renal function without apparent adverse effects on the liver, and alleviated renal pathological lesions. Triptolide also elevated the nephrin protein level. Furthermore, levels of miR-344b-3p and miR-30b-3p were elevated in rats with adriamycin-induced nephropathy, while triptolide treatment reversed the increase in the expression of these two miRNAs. Conclusions. These results suggest that triptolide may attenuate podocyte injury in rats with adriamycin-induced nephropathy by regulating expression of miRNA-344b-3p and miRNA-30b-3p.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2015/107814</identifier><identifier>PMID: 26078766</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Animals ; Biological products ; Care and treatment ; Development and progression ; Diterpenes ; Drug dosages ; Gene expression ; Genetic aspects ; Health aspects ; Hospitals ; Kidney diseases ; Medicine ; Metabolism ; MicroRNA ; Physiology ; Properties ; Proteins ; Rodents ; Studies ; Urine</subject><ispartof>Evidence-based complementary and alternative medicine, 2015-01, Vol.2015 (2015), p.1-13</ispartof><rights>Copyright © 2015 Chun-Bo Jiang et al.</rights><rights>COPYRIGHT 2015 John Wiley & Sons, Inc.</rights><rights>Copyright © 2015 Chun-Bo Jiang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2015 Chun-Bo Jiang et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-967bcd6e58cdb7ae25ca5f07ea1da2363a05200cd5c9e75f51e7d424535f686b3</citedby><cites>FETCH-LOGICAL-c495t-967bcd6e58cdb7ae25ca5f07ea1da2363a05200cd5c9e75f51e7d424535f686b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452866/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452866/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26078766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Schmeda-Hirschmann, Guillermo</contributor><creatorcontrib>Sun, Wei</creatorcontrib><creatorcontrib>Li, Chun-Qing</creatorcontrib><creatorcontrib>Zhu, Hao</creatorcontrib><creatorcontrib>Tu, Yue</creatorcontrib><creatorcontrib>Wei, Ming-Gang</creatorcontrib><creatorcontrib>Jiang, Chun-Bo</creatorcontrib><creatorcontrib>Jing, Wei-Min</creatorcontrib><title>Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Objectives. We investigated the action of triptolide in rats with adriamycin-induced nephropathy and evaluated the possible mechanisms underlying its protective effect against podocyte injury. Methods. In total, 30 healthy male Sprague-Dawley rats were randomized into three groups (normal group, model group, and triptolide group). On days 7, 28, 42, and 56, 24 h urine samples were collected. All rats were sacrificed on day 56, and their blood and renal tissues were collected for determination of biochemical and molecular biological parameters. Expression of miRNAs in the renal cortex was analyzed by a biochip assay and RT-PCR was used to confirm observed differences in miRNA levels. Results. Triptolide decreased proteinuria, improved renal function without apparent adverse effects on the liver, and alleviated renal pathological lesions. Triptolide also elevated the nephrin protein level. Furthermore, levels of miR-344b-3p and miR-30b-3p were elevated in rats with adriamycin-induced nephropathy, while triptolide treatment reversed the increase in the expression of these two miRNAs. Conclusions. These results suggest that triptolide may attenuate podocyte injury in rats with adriamycin-induced nephropathy by regulating expression of miRNA-344b-3p and miRNA-30b-3p.</description><subject>Animals</subject><subject>Biological products</subject><subject>Care and treatment</subject><subject>Development and progression</subject><subject>Diterpenes</subject><subject>Drug dosages</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Hospitals</subject><subject>Kidney diseases</subject><subject>Medicine</subject><subject>Metabolism</subject><subject>MicroRNA</subject><subject>Physiology</subject><subject>Properties</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Studies</subject><subject>Urine</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkk9rFDEYhwdRbK2evEvAiyhjk0z-zFyEpVRdKFWWCt5CJnlnN2U2GZOMdb6DH9pZt12rJ08Jbx6eN2_yK4rnBL8lhPNTigk_JVjWhD0ojolkpGS0rh8e9vLrUfEkpWuMaSOlfFwcUTHjUojj4udVdEMOvbOAFjmDH3WGhD4HG8yUAS399Rgn1E5oBeux19n5NTr_MURIyQWPQoe2bnW5KCvG2rIakPb2roJ_F5xHK50TunF5gxY2Or2djPPl0tvRgEWXMGxiGHTeTE-LR53uEzy7XU-KL-_Pr84-lhefPizPFhelYQ3PZSNka6wAXhvbSg2UG807LEETq2klKo05xdhYbhqQvOMEpGWU8Yp3ohZtdVK823uHsd2CNeBz1L0aotvqOKmgnfr7xLuNWofvijFOayFmwatbQQzfRkhZbV0y0PfaQxiTIqJuBKlrSWb05T_odRijn8dTROKGNphI-oda6x6U812Y-5qdVC0YrwXjRO7avtlTJoaUInSHKxOsdllQuyyofRZm-sX9KQ_s3efPwOs9sHHe6hv3fzaYEej0PVgQMr_tL3_VxQ4</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Sun, Wei</creator><creator>Li, Chun-Qing</creator><creator>Zhu, Hao</creator><creator>Tu, Yue</creator><creator>Wei, Ming-Gang</creator><creator>Jiang, Chun-Bo</creator><creator>Jing, Wei-Min</creator><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy</title><author>Sun, Wei ; Li, Chun-Qing ; Zhu, Hao ; Tu, Yue ; Wei, Ming-Gang ; Jiang, Chun-Bo ; Jing, Wei-Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-967bcd6e58cdb7ae25ca5f07ea1da2363a05200cd5c9e75f51e7d424535f686b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Biological products</topic><topic>Care and treatment</topic><topic>Development and progression</topic><topic>Diterpenes</topic><topic>Drug dosages</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Hospitals</topic><topic>Kidney diseases</topic><topic>Medicine</topic><topic>Metabolism</topic><topic>MicroRNA</topic><topic>Physiology</topic><topic>Properties</topic><topic>Proteins</topic><topic>Rodents</topic><topic>Studies</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Wei</creatorcontrib><creatorcontrib>Li, Chun-Qing</creatorcontrib><creatorcontrib>Zhu, Hao</creatorcontrib><creatorcontrib>Tu, Yue</creatorcontrib><creatorcontrib>Wei, Ming-Gang</creatorcontrib><creatorcontrib>Jiang, Chun-Bo</creatorcontrib><creatorcontrib>Jing, Wei-Min</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Psychology Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Wei</au><au>Li, Chun-Qing</au><au>Zhu, Hao</au><au>Tu, Yue</au><au>Wei, Ming-Gang</au><au>Jiang, Chun-Bo</au><au>Jing, Wei-Min</au><au>Schmeda-Hirschmann, Guillermo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>2015</volume><issue>2015</issue><spage>1</spage><epage>13</epage><pages>1-13</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Objectives. We investigated the action of triptolide in rats with adriamycin-induced nephropathy and evaluated the possible mechanisms underlying its protective effect against podocyte injury. Methods. In total, 30 healthy male Sprague-Dawley rats were randomized into three groups (normal group, model group, and triptolide group). On days 7, 28, 42, and 56, 24 h urine samples were collected. All rats were sacrificed on day 56, and their blood and renal tissues were collected for determination of biochemical and molecular biological parameters. Expression of miRNAs in the renal cortex was analyzed by a biochip assay and RT-PCR was used to confirm observed differences in miRNA levels. Results. Triptolide decreased proteinuria, improved renal function without apparent adverse effects on the liver, and alleviated renal pathological lesions. Triptolide also elevated the nephrin protein level. Furthermore, levels of miR-344b-3p and miR-30b-3p were elevated in rats with adriamycin-induced nephropathy, while triptolide treatment reversed the increase in the expression of these two miRNAs. Conclusions. These results suggest that triptolide may attenuate podocyte injury in rats with adriamycin-induced nephropathy by regulating expression of miRNA-344b-3p and miRNA-30b-3p.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>26078766</pmid><doi>10.1155/2015/107814</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological products Care and treatment Development and progression Diterpenes Drug dosages Gene expression Genetic aspects Health aspects Hospitals Kidney diseases Medicine Metabolism MicroRNA Physiology Properties Proteins Rodents Studies Urine |
| title | Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy |
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