Chronic disuse and skeletal muscle structure in older adults: sex-specific differences and relationships to contractile function
In older adults, we examined the effect of chronic muscle disuse on skeletal muscle structure at the tissue, cellular, organellar, and molecular levels and its relationship to muscle function. Volunteers with advanced-stage knee osteoarthritis (OA, n = 16) were recruited to reflect the effects of ch...
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| Veröffentlicht in: | American Journal of Physiology: Cell Physiology 2015-06, Vol.308 (11), p.C932-C943 |
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| creator | Callahan, Damien M Tourville, Timothy W Miller, Mark S Hackett, Sarah B Sharma, Himani Cruickshank, Nicholas C Slauterbeck, James R Savage, Patrick D Ades, Philip A Maughan, David W Beynnon, Bruce D Toth, Michael J |
| description | In older adults, we examined the effect of chronic muscle disuse on skeletal muscle structure at the tissue, cellular, organellar, and molecular levels and its relationship to muscle function. Volunteers with advanced-stage knee osteoarthritis (OA, n = 16) were recruited to reflect the effects of chronic lower extremity muscle disuse and compared with recreationally active controls (n = 15) without knee OA but similar in age, sex, and health status. In the OA group, quadriceps muscle and single-fiber cross-sectional area were reduced, with the largest reduction in myosin heavy chain IIA fibers. Myosin heavy chain IIAX fibers were more prevalent in the OA group, and their atrophy was sex-specific: men showed a reduction in cross-sectional area, and women showed no differences. Myofibrillar ultrastructure, myonuclear content, and mitochondrial content and morphology generally did not differ between groups, with the exception of sex-specific adaptations in subsarcolemmal (SS) mitochondria, which were driven by lower values in OA women. SS mitochondrial content was also differently related to cellular and molecular functional parameters by sex: greater SS mitochondrial content was associated with improved contractility in women but reduced function in men. Collectively, these results demonstrate sex-specific structural phenotypes at the cellular and organellar levels with chronic disuse in older adults, with novel associations between energetic and contractile systems. |
| doi_str_mv | 10.1152/ajpcell.00014.2015 |
| format | Article |
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Volunteers with advanced-stage knee osteoarthritis (OA, n = 16) were recruited to reflect the effects of chronic lower extremity muscle disuse and compared with recreationally active controls (n = 15) without knee OA but similar in age, sex, and health status. In the OA group, quadriceps muscle and single-fiber cross-sectional area were reduced, with the largest reduction in myosin heavy chain IIA fibers. Myosin heavy chain IIAX fibers were more prevalent in the OA group, and their atrophy was sex-specific: men showed a reduction in cross-sectional area, and women showed no differences. Myofibrillar ultrastructure, myonuclear content, and mitochondrial content and morphology generally did not differ between groups, with the exception of sex-specific adaptations in subsarcolemmal (SS) mitochondria, which were driven by lower values in OA women. SS mitochondrial content was also differently related to cellular and molecular functional parameters by sex: greater SS mitochondrial content was associated with improved contractility in women but reduced function in men. Collectively, these results demonstrate sex-specific structural phenotypes at the cellular and organellar levels with chronic disuse in older adults, with novel associations between energetic and contractile systems.</description><identifier>ISSN: 0363-6143</identifier><identifier>EISSN: 1522-1563</identifier><identifier>DOI: 10.1152/ajpcell.00014.2015</identifier><identifier>PMID: 25810256</identifier><identifier>CODEN: AJPCDD</identifier><language>eng</language><publisher>United States: American Physiological Society</publisher><subject>Aged ; Case-Control Studies ; Cellular biology ; Exercise ; Female ; Gender differences ; Gene Expression ; Humans ; Knee - pathology ; Knee - physiopathology ; Male ; Mitochondria ; Mitochondria - metabolism ; Mitochondria - ultrastructure ; Morphology ; Muscle Contraction ; Muscle Fibers, Skeletal - metabolism ; Muscle Fibers, Skeletal - pathology ; Muscle Fibers, Skeletal - ultrastructure ; Muscular Atrophy - complications ; Muscular Atrophy - metabolism ; Muscular Atrophy - pathology ; Muscular Atrophy - physiopathology ; Myosin Heavy Chains - genetics ; Myosin Heavy Chains - metabolism ; Older people ; Osteoarthritis, Knee - complications ; Osteoarthritis, Knee - metabolism ; Osteoarthritis, Knee - pathology ; Osteoarthritis, Knee - physiopathology ; Protein Isoforms - genetics ; Protein Isoforms - metabolism ; Quadriceps Muscle - metabolism ; Quadriceps Muscle - physiopathology ; Quadriceps Muscle - ultrastructure ; Sex Factors ; Skeletal system</subject><ispartof>American Journal of Physiology: Cell Physiology, 2015-06, Vol.308 (11), p.C932-C943</ispartof><rights>Copyright © 2015 the American Physiological Society.</rights><rights>Copyright American Physiological Society Jun 1, 2015</rights><rights>Copyright © 2015 the American Physiological Society 2015 American Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-3271b70a690e77a8aec49f4e477da4e36796331dcba31e91473e71536d65aa8e3</citedby><cites>FETCH-LOGICAL-c463t-3271b70a690e77a8aec49f4e477da4e36796331dcba31e91473e71536d65aa8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,781,785,886,3040,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25810256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Callahan, Damien M</creatorcontrib><creatorcontrib>Tourville, Timothy W</creatorcontrib><creatorcontrib>Miller, Mark S</creatorcontrib><creatorcontrib>Hackett, Sarah B</creatorcontrib><creatorcontrib>Sharma, Himani</creatorcontrib><creatorcontrib>Cruickshank, Nicholas C</creatorcontrib><creatorcontrib>Slauterbeck, James R</creatorcontrib><creatorcontrib>Savage, Patrick D</creatorcontrib><creatorcontrib>Ades, Philip A</creatorcontrib><creatorcontrib>Maughan, David W</creatorcontrib><creatorcontrib>Beynnon, Bruce D</creatorcontrib><creatorcontrib>Toth, Michael J</creatorcontrib><title>Chronic disuse and skeletal muscle structure in older adults: sex-specific differences and relationships to contractile function</title><title>American Journal of Physiology: Cell Physiology</title><addtitle>Am J Physiol Cell Physiol</addtitle><description>In older adults, we examined the effect of chronic muscle disuse on skeletal muscle structure at the tissue, cellular, organellar, and molecular levels and its relationship to muscle function. Volunteers with advanced-stage knee osteoarthritis (OA, n = 16) were recruited to reflect the effects of chronic lower extremity muscle disuse and compared with recreationally active controls (n = 15) without knee OA but similar in age, sex, and health status. In the OA group, quadriceps muscle and single-fiber cross-sectional area were reduced, with the largest reduction in myosin heavy chain IIA fibers. Myosin heavy chain IIAX fibers were more prevalent in the OA group, and their atrophy was sex-specific: men showed a reduction in cross-sectional area, and women showed no differences. Myofibrillar ultrastructure, myonuclear content, and mitochondrial content and morphology generally did not differ between groups, with the exception of sex-specific adaptations in subsarcolemmal (SS) mitochondria, which were driven by lower values in OA women. SS mitochondrial content was also differently related to cellular and molecular functional parameters by sex: greater SS mitochondrial content was associated with improved contractility in women but reduced function in men. Collectively, these results demonstrate sex-specific structural phenotypes at the cellular and organellar levels with chronic disuse in older adults, with novel associations between energetic and contractile systems.</description><subject>Aged</subject><subject>Case-Control Studies</subject><subject>Cellular biology</subject><subject>Exercise</subject><subject>Female</subject><subject>Gender differences</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Knee - pathology</subject><subject>Knee - physiopathology</subject><subject>Male</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondria - ultrastructure</subject><subject>Morphology</subject><subject>Muscle Contraction</subject><subject>Muscle Fibers, Skeletal - metabolism</subject><subject>Muscle Fibers, Skeletal - pathology</subject><subject>Muscle Fibers, Skeletal - ultrastructure</subject><subject>Muscular Atrophy - complications</subject><subject>Muscular Atrophy - metabolism</subject><subject>Muscular Atrophy - pathology</subject><subject>Muscular Atrophy - physiopathology</subject><subject>Myosin Heavy Chains - genetics</subject><subject>Myosin Heavy Chains - metabolism</subject><subject>Older people</subject><subject>Osteoarthritis, Knee - complications</subject><subject>Osteoarthritis, Knee - metabolism</subject><subject>Osteoarthritis, Knee - pathology</subject><subject>Osteoarthritis, Knee - physiopathology</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Isoforms - metabolism</subject><subject>Quadriceps Muscle - metabolism</subject><subject>Quadriceps Muscle - physiopathology</subject><subject>Quadriceps Muscle - ultrastructure</subject><subject>Sex Factors</subject><subject>Skeletal system</subject><issn>0363-6143</issn><issn>1522-1563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkj1vFDEQhi0EIpfAH6BAlmho9rDXX7sUSOhEAClSmlBbPu8s58NnL_5A0PHT2b0cEUlD5WKeeTwevwi9oGRNqWjfmP1kwfs1IYTydUuoeIRWc6FtqJDsMVoRJlkjKWdn6Dzn_czxVvZP0VkrOkpaIVfo92aXYnAWDy7XDNiEAedv4KEYjw81Ww84l1RtqQmwCzj6ARI2Q_Ulv8UZfjZ5AuvGo2IcIUGwkI-eBN4UF0PeuSnjErGNoSRji5ulYw12KT5DT0bjMzw_nRfoy-WHm82n5ur64-fN-6vGcslKw1pFt4oY2RNQynQGLO9HDlypwXBgUvWSMTrYrWEUesoVA0UFk4MUxnTALtC7W-9UtwcYLCyjeD0ldzDpl47G6fuV4Hb6a_yhOReU8W4WvD4JUvxeIRd9cHnZvwkQa9a0a1nPGRPk_6jshBKCCDajrx6g-1hTmDexUB0RRB3vbm8pm2LOCca7uSnRSxb0KQv6mAW9ZGFuevnvi-9a_n4--wNqO7QP</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Callahan, Damien M</creator><creator>Tourville, Timothy W</creator><creator>Miller, Mark S</creator><creator>Hackett, Sarah B</creator><creator>Sharma, Himani</creator><creator>Cruickshank, Nicholas C</creator><creator>Slauterbeck, James R</creator><creator>Savage, Patrick D</creator><creator>Ades, Philip A</creator><creator>Maughan, David W</creator><creator>Beynnon, Bruce D</creator><creator>Toth, Michael J</creator><general>American Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TS</scope><scope>7X8</scope><scope>7U7</scope><scope>C1K</scope><scope>5PM</scope></search><sort><creationdate>20150601</creationdate><title>Chronic disuse and skeletal muscle structure in older adults: sex-specific differences and relationships to contractile function</title><author>Callahan, Damien M ; 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Volunteers with advanced-stage knee osteoarthritis (OA, n = 16) were recruited to reflect the effects of chronic lower extremity muscle disuse and compared with recreationally active controls (n = 15) without knee OA but similar in age, sex, and health status. In the OA group, quadriceps muscle and single-fiber cross-sectional area were reduced, with the largest reduction in myosin heavy chain IIA fibers. Myosin heavy chain IIAX fibers were more prevalent in the OA group, and their atrophy was sex-specific: men showed a reduction in cross-sectional area, and women showed no differences. Myofibrillar ultrastructure, myonuclear content, and mitochondrial content and morphology generally did not differ between groups, with the exception of sex-specific adaptations in subsarcolemmal (SS) mitochondria, which were driven by lower values in OA women. SS mitochondrial content was also differently related to cellular and molecular functional parameters by sex: greater SS mitochondrial content was associated with improved contractility in women but reduced function in men. Collectively, these results demonstrate sex-specific structural phenotypes at the cellular and organellar levels with chronic disuse in older adults, with novel associations between energetic and contractile systems.</abstract><cop>United States</cop><pub>American Physiological Society</pub><pmid>25810256</pmid><doi>10.1152/ajpcell.00014.2015</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | American Journal of Physiology: Cell Physiology, 2015-06, Vol.308 (11), p.C932-C943 |
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| subjects | Aged Case-Control Studies Cellular biology Exercise Female Gender differences Gene Expression Humans Knee - pathology Knee - physiopathology Male Mitochondria Mitochondria - metabolism Mitochondria - ultrastructure Morphology Muscle Contraction Muscle Fibers, Skeletal - metabolism Muscle Fibers, Skeletal - pathology Muscle Fibers, Skeletal - ultrastructure Muscular Atrophy - complications Muscular Atrophy - metabolism Muscular Atrophy - pathology Muscular Atrophy - physiopathology Myosin Heavy Chains - genetics Myosin Heavy Chains - metabolism Older people Osteoarthritis, Knee - complications Osteoarthritis, Knee - metabolism Osteoarthritis, Knee - pathology Osteoarthritis, Knee - physiopathology Protein Isoforms - genetics Protein Isoforms - metabolism Quadriceps Muscle - metabolism Quadriceps Muscle - physiopathology Quadriceps Muscle - ultrastructure Sex Factors Skeletal system |
| title | Chronic disuse and skeletal muscle structure in older adults: sex-specific differences and relationships to contractile function |
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