A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate
APSL (active peptide from shark liver) is a hepatic stimulator cytokine from the liver of Chiloscyllium. It can effectively protect islet cells and improve complications in mice with alloxan-induced diabetes. Here, we demonstrate that the APSL sequence is present in the N-terminus of novel TBC (Tre-...
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| Veröffentlicht in: | Marine drugs 2015-05, Vol.13 (5), p.2955-2966 |
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| creator | Li, Yuanyuan Wang, Weidong Cheng, Dandan Wang, Tao Lu, Conger Chen, Jian Nie, Zuoming Zhang, Wenping Lv, Zhengbing Wu, Wutong Shu, Jianhong |
| description | APSL (active peptide from shark liver) is a hepatic stimulator cytokine from the liver of Chiloscyllium. It can effectively protect islet cells and improve complications in mice with alloxan-induced diabetes. Here, we demonstrate that the APSL sequence is present in the N-terminus of novel TBC (Tre-2, Bub2 and Cdc16) domain family, member 15 (TBC1D15) from Chiloscyllium plagiosum. This shark TBC1D15 gene, which contains an ORF of 2088 bp, was identified from a cDNA library of regenerating shark liver. Bioinformatic analysis showed that the gene is highly homologous to TBC1D15 genes from other species. Moreover, the N-terminus of shark TBC1D15 contains a motif of unknown function (DUF3548), which encompasses the APSL fragment. Rab-GAP activity analysis showed that shark TBC1D15 is a new member of the TBC1D15 family. These results demonstrated that shark TBC1D15 possesses Rab-GAP activity using Rab7 as a substrate, which is a common property of the TBC1D15 family. The involvement of APSL at the N-terminus of TBC1D15 also demonstrates that this protein might be involved in insulin signaling and may be associated with the development of type 2 diabetes. The current findings pave the way for further functional and clinical studies of these proteins from marine sources. |
| doi_str_mv | 10.3390/md13052955 |
| format | Article |
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It can effectively protect islet cells and improve complications in mice with alloxan-induced diabetes. Here, we demonstrate that the APSL sequence is present in the N-terminus of novel TBC (Tre-2, Bub2 and Cdc16) domain family, member 15 (TBC1D15) from Chiloscyllium plagiosum. This shark TBC1D15 gene, which contains an ORF of 2088 bp, was identified from a cDNA library of regenerating shark liver. Bioinformatic analysis showed that the gene is highly homologous to TBC1D15 genes from other species. Moreover, the N-terminus of shark TBC1D15 contains a motif of unknown function (DUF3548), which encompasses the APSL fragment. Rab-GAP activity analysis showed that shark TBC1D15 is a new member of the TBC1D15 family. These results demonstrated that shark TBC1D15 possesses Rab-GAP activity using Rab7 as a substrate, which is a common property of the TBC1D15 family. The involvement of APSL at the N-terminus of TBC1D15 also demonstrates that this protein might be involved in insulin signaling and may be associated with the development of type 2 diabetes. The current findings pave the way for further functional and clinical studies of these proteins from marine sources.</description><identifier>ISSN: 1660-3397</identifier><identifier>EISSN: 1660-3397</identifier><identifier>DOI: 10.3390/md13052955</identifier><identifier>PMID: 25984991</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Amino Acid Sequence ; Animals ; Chiloscyllium ; Chiloscyllium plagiosum ; Diabetes Mellitus, Type 2 - metabolism ; Gene Library ; GTPase-Activating Proteins - metabolism ; Liver - metabolism ; Molecular Sequence Data ; Protein Structure, Tertiary ; rab GTP-Binding Proteins - metabolism ; Sequence Alignment ; Sharks - metabolism ; Signal Transduction - physiology</subject><ispartof>Marine drugs, 2015-05, Vol.13 (5), p.2955-2966</ispartof><rights>Copyright MDPI AG 2015</rights><rights>2015 by the authors; licensee MDPI, Basel, Switzerland. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-7feea4f8fab238720f80ceeb9a24a35b6c6eb35561210737871e664d6b0fb3f33</citedby><cites>FETCH-LOGICAL-c439t-7feea4f8fab238720f80ceeb9a24a35b6c6eb35561210737871e664d6b0fb3f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446614/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446614/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27915,27916,53782,53784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25984991$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yuanyuan</creatorcontrib><creatorcontrib>Wang, Weidong</creatorcontrib><creatorcontrib>Cheng, Dandan</creatorcontrib><creatorcontrib>Wang, Tao</creatorcontrib><creatorcontrib>Lu, Conger</creatorcontrib><creatorcontrib>Chen, Jian</creatorcontrib><creatorcontrib>Nie, Zuoming</creatorcontrib><creatorcontrib>Zhang, Wenping</creatorcontrib><creatorcontrib>Lv, Zhengbing</creatorcontrib><creatorcontrib>Wu, Wutong</creatorcontrib><creatorcontrib>Shu, Jianhong</creatorcontrib><title>A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate</title><title>Marine drugs</title><addtitle>Mar Drugs</addtitle><description>APSL (active peptide from shark liver) is a hepatic stimulator cytokine from the liver of Chiloscyllium. It can effectively protect islet cells and improve complications in mice with alloxan-induced diabetes. Here, we demonstrate that the APSL sequence is present in the N-terminus of novel TBC (Tre-2, Bub2 and Cdc16) domain family, member 15 (TBC1D15) from Chiloscyllium plagiosum. This shark TBC1D15 gene, which contains an ORF of 2088 bp, was identified from a cDNA library of regenerating shark liver. Bioinformatic analysis showed that the gene is highly homologous to TBC1D15 genes from other species. Moreover, the N-terminus of shark TBC1D15 contains a motif of unknown function (DUF3548), which encompasses the APSL fragment. Rab-GAP activity analysis showed that shark TBC1D15 is a new member of the TBC1D15 family. These results demonstrated that shark TBC1D15 possesses Rab-GAP activity using Rab7 as a substrate, which is a common property of the TBC1D15 family. The involvement of APSL at the N-terminus of TBC1D15 also demonstrates that this protein might be involved in insulin signaling and may be associated with the development of type 2 diabetes. The current findings pave the way for further functional and clinical studies of these proteins from marine sources.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Chiloscyllium</subject><subject>Chiloscyllium plagiosum</subject><subject>Diabetes Mellitus, Type 2 - metabolism</subject><subject>Gene Library</subject><subject>GTPase-Activating Proteins - metabolism</subject><subject>Liver - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Protein Structure, Tertiary</subject><subject>rab GTP-Binding Proteins - metabolism</subject><subject>Sequence Alignment</subject><subject>Sharks - metabolism</subject><subject>Signal Transduction - physiology</subject><issn>1660-3397</issn><issn>1660-3397</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1v1DAQhiMEoqVw4QcgS1wQUlp_O-ZQabvQUqlABcs5srPjXVdOvNhJ0Yo_X69aSuHCaUYzj975eKvqJcGHjGl81C8Jw4JqIR5V-0RKXJeyevwg36ue5XyFMRON5k-rPSp0w7Um-9WvGfoMP9En6C0kFB0a14AWJ3Pyngh0anoftsil2KP52oeYu20IfurRJpiVj3nq36GvxqKzxaXJUM-60V-b0Q8rdJniCH5AJ6W-RHHYYQqZjAz6Ntk8JjPC8-qJMyHDi7t4UH0__bCYf6wvvpydz2cXdceZHmvlAAx3jTOWskZR7BrcAVhtKDdMWNlJsEwISSjBiqlGEZCSL6XFzjLH2EF1fKu7mWwPyw6GMj60m-R7k7ZtNL79uzP4dbuK1y3nXErCi8CbO4EUf0yQx7b3uYMQzABxyi1RmPBGaYL_j8qGUsyl2q31-h_0Kk5pKJ8olC5-FogW6u0t1aWYcwJ3vzfB7c7-9o_9BX718NJ79Lff7Abb_akz</recordid><startdate>20150513</startdate><enddate>20150513</enddate><creator>Li, Yuanyuan</creator><creator>Wang, Weidong</creator><creator>Cheng, Dandan</creator><creator>Wang, Tao</creator><creator>Lu, Conger</creator><creator>Chen, Jian</creator><creator>Nie, Zuoming</creator><creator>Zhang, Wenping</creator><creator>Lv, Zhengbing</creator><creator>Wu, Wutong</creator><creator>Shu, Jianhong</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T7</scope><scope>7TN</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H95</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.F</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150513</creationdate><title>A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate</title><author>Li, Yuanyuan ; Wang, Weidong ; Cheng, Dandan ; Wang, Tao ; Lu, Conger ; Chen, Jian ; Nie, Zuoming ; Zhang, Wenping ; Lv, Zhengbing ; Wu, Wutong ; Shu, Jianhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-7feea4f8fab238720f80ceeb9a24a35b6c6eb35561210737871e664d6b0fb3f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Chiloscyllium</topic><topic>Chiloscyllium plagiosum</topic><topic>Diabetes Mellitus, Type 2 - metabolism</topic><topic>Gene Library</topic><topic>GTPase-Activating Proteins - metabolism</topic><topic>Liver - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Protein Structure, Tertiary</topic><topic>rab GTP-Binding Proteins - metabolism</topic><topic>Sequence Alignment</topic><topic>Sharks - metabolism</topic><topic>Signal Transduction - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Yuanyuan</creatorcontrib><creatorcontrib>Wang, Weidong</creatorcontrib><creatorcontrib>Cheng, Dandan</creatorcontrib><creatorcontrib>Wang, Tao</creatorcontrib><creatorcontrib>Lu, Conger</creatorcontrib><creatorcontrib>Chen, Jian</creatorcontrib><creatorcontrib>Nie, Zuoming</creatorcontrib><creatorcontrib>Zhang, Wenping</creatorcontrib><creatorcontrib>Lv, Zhengbing</creatorcontrib><creatorcontrib>Wu, Wutong</creatorcontrib><creatorcontrib>Shu, Jianhong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Marine drugs</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yuanyuan</au><au>Wang, Weidong</au><au>Cheng, Dandan</au><au>Wang, Tao</au><au>Lu, Conger</au><au>Chen, Jian</au><au>Nie, Zuoming</au><au>Zhang, Wenping</au><au>Lv, Zhengbing</au><au>Wu, Wutong</au><au>Shu, Jianhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate</atitle><jtitle>Marine drugs</jtitle><addtitle>Mar Drugs</addtitle><date>2015-05-13</date><risdate>2015</risdate><volume>13</volume><issue>5</issue><spage>2955</spage><epage>2966</epage><pages>2955-2966</pages><issn>1660-3397</issn><eissn>1660-3397</eissn><abstract>APSL (active peptide from shark liver) is a hepatic stimulator cytokine from the liver of Chiloscyllium. It can effectively protect islet cells and improve complications in mice with alloxan-induced diabetes. Here, we demonstrate that the APSL sequence is present in the N-terminus of novel TBC (Tre-2, Bub2 and Cdc16) domain family, member 15 (TBC1D15) from Chiloscyllium plagiosum. This shark TBC1D15 gene, which contains an ORF of 2088 bp, was identified from a cDNA library of regenerating shark liver. Bioinformatic analysis showed that the gene is highly homologous to TBC1D15 genes from other species. Moreover, the N-terminus of shark TBC1D15 contains a motif of unknown function (DUF3548), which encompasses the APSL fragment. Rab-GAP activity analysis showed that shark TBC1D15 is a new member of the TBC1D15 family. These results demonstrated that shark TBC1D15 possesses Rab-GAP activity using Rab7 as a substrate, which is a common property of the TBC1D15 family. The involvement of APSL at the N-terminus of TBC1D15 also demonstrates that this protein might be involved in insulin signaling and may be associated with the development of type 2 diabetes. The current findings pave the way for further functional and clinical studies of these proteins from marine sources.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>25984991</pmid><doi>10.3390/md13052955</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Full-Text Journals in Chemistry (Open access); DOAJ Directory of Open Access Journals; PubMed Central; EZB Electronic Journals Library; PubMed Central Open Access |
| subjects | Amino Acid Sequence Animals Chiloscyllium Chiloscyllium plagiosum Diabetes Mellitus, Type 2 - metabolism Gene Library GTPase-Activating Proteins - metabolism Liver - metabolism Molecular Sequence Data Protein Structure, Tertiary rab GTP-Binding Proteins - metabolism Sequence Alignment Sharks - metabolism Signal Transduction - physiology |
| title | A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate |
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