Simultaneous determination of major type A and B trichothecenes, zearalenone and certain modified metabolites in Finnish cereal grains with a novel liquid chromatography-tandem mass spectrometric method
A reliable and sensitive liquid chromatography-tandem mass spectrometric method was developed for the simultaneous quantitative determination in cereals of the Fusarium mycotoxins HT-2 toxin, T-2 toxin, deoxynivalenol, nivalenol and zearalenone, as well as the modified metabolites 3-acetyl-deoxyniva...
Gespeichert in:
| Veröffentlicht in: | Analytical and bioanalytical chemistry 2015-06, Vol.407 (16), p.4745-4755 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4755 |
|---|---|
| container_issue | 16 |
| container_start_page | 4745 |
| container_title | Analytical and bioanalytical chemistry |
| container_volume | 407 |
| creator | Nathanail, Alexis V. Syvähuoko, Jenna Malachová, Alexandra Jestoi, Marika Varga, Elisabeth Michlmayr, Herbert Adam, Gerhard Sieviläinen, Elina Berthiller, Franz Peltonen, Kimmo |
| description | A reliable and sensitive liquid chromatography-tandem mass spectrometric method was developed for the simultaneous quantitative determination in cereals of the
Fusarium
mycotoxins HT-2 toxin, T-2 toxin, deoxynivalenol, nivalenol and zearalenone, as well as the modified metabolites 3-acetyl-deoxynivalenol, α-zearalenol, β-zearalenol, deoxynivalenol-3-glucoside, HT-2-3-glucoside, nivalenol-3-glucoside, zearalenone-14-glucoside, zearalenone-14-sulphate, zearalenone-16-glucoside, α-zearalenol-14-glucoside and β-zearalenol-14-glucoside. The ‘dilute and shoot’ approach was used for sample preparation after extraction with acetonitrile:water:acetic acid (79:20:1,
v
/
v
/
v
). Separation was carried out using reversed-phase liquid chromatography, and detection was performed using tandem mass spectrometry in the selected reaction monitoring mode. The method was in-house validated according to performance characteristics, established in Commission Regulation EC No 401/2006 and Commission Decision EC No 657/2002, prior to its application in a nationwide survey for the analysis of barley, oat and wheat samples (
n
= 95) harvested in Finland during 2013. Deoxynivalenol and its glucosylated form were the most abundant of the analytes, being detected in 93 and 81 % of the samples, respectively. Concentrations of deoxynivalenol were unusually high in 2013, especially in oats, with some cases exceeding the maximum legislative limits for unprocessed oats placed on the market for first-stage processing. All modified mycotoxins analysed were detected, and the natural occurrence of some of these compounds (e.g. zearalenone-16-glucoside and nivalenol-3-glucoside) in barley, oats and/or wheat was documented for the first time. |
| doi_str_mv | 10.1007/s00216-015-8676-4 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4446524</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A435385266</galeid><sourcerecordid>A435385266</sourcerecordid><originalsourceid>FETCH-LOGICAL-c715t-3a2643339bd765b35d8f0dad85d55463120d572837908d3a541f9cdfa088fa003</originalsourceid><addsrcrecordid>eNqNkt-K1DAUxoso7jr6AN5IwBsv7Jo0f9reCOPiqrDghXodMs3pNEubzCbpyviIPpWnzjqsgiiFtuT88uXkO19RPGX0jFFav0qUVkyVlMmyUbUqxb3ilCnWlJWS9P7xX1QnxaOUriiCDVMPi5NKtlyqmp0W3z-5aR6z8RDmRCxkiJPzJrvgSejJZK5CJHm_A7ImxlvyhuTouiHkATrwkF6Sb2CiGcEHDz-JDmI2zpMpWNc7sGSCbDZhdBkSwfUL571Lw8KBGck2IpzIV5cHYogPNzCS0V3PDpWGGCaTAyK7YV9ikxYmbCklknbQZazC0s1ywhDs4-JBb8YET26_q-LLxdvP5-_Ly4_vPpyvL8uuZjKX3KAjnPN2Y2slN1zapqfW2EZaKYXirKJW1lXD65Y2lhspWN92tje0afBF-ap4fdDdzZsJLNqQ0QC9i24yca-Dcfr3ineD3oYbLYRQshIo8OJWIIbrGVLWk0sdjONhCprVQlVtK7DLf6OsaZUQ9H9UKRMN43jFVfH8D_QqzNGjaUgtHsi2XgTPDtQWx6ud7wPepsMHp-A6HHfvcH0tuOSNrNQiyw4buhhSitAfPWFUL4HVh8BqzKFeAquXQ57dNfO441dCEagOQMKS30K80-tfVX8AYR36OA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1754635974</pqid></control><display><type>article</type><title>Simultaneous determination of major type A and B trichothecenes, zearalenone and certain modified metabolites in Finnish cereal grains with a novel liquid chromatography-tandem mass spectrometric method</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Nathanail, Alexis V. ; Syvähuoko, Jenna ; Malachová, Alexandra ; Jestoi, Marika ; Varga, Elisabeth ; Michlmayr, Herbert ; Adam, Gerhard ; Sieviläinen, Elina ; Berthiller, Franz ; Peltonen, Kimmo</creator><creatorcontrib>Nathanail, Alexis V. ; Syvähuoko, Jenna ; Malachová, Alexandra ; Jestoi, Marika ; Varga, Elisabeth ; Michlmayr, Herbert ; Adam, Gerhard ; Sieviläinen, Elina ; Berthiller, Franz ; Peltonen, Kimmo</creatorcontrib><description>A reliable and sensitive liquid chromatography-tandem mass spectrometric method was developed for the simultaneous quantitative determination in cereals of the
Fusarium
mycotoxins HT-2 toxin, T-2 toxin, deoxynivalenol, nivalenol and zearalenone, as well as the modified metabolites 3-acetyl-deoxynivalenol, α-zearalenol, β-zearalenol, deoxynivalenol-3-glucoside, HT-2-3-glucoside, nivalenol-3-glucoside, zearalenone-14-glucoside, zearalenone-14-sulphate, zearalenone-16-glucoside, α-zearalenol-14-glucoside and β-zearalenol-14-glucoside. The ‘dilute and shoot’ approach was used for sample preparation after extraction with acetonitrile:water:acetic acid (79:20:1,
v
/
v
/
v
). Separation was carried out using reversed-phase liquid chromatography, and detection was performed using tandem mass spectrometry in the selected reaction monitoring mode. The method was in-house validated according to performance characteristics, established in Commission Regulation EC No 401/2006 and Commission Decision EC No 657/2002, prior to its application in a nationwide survey for the analysis of barley, oat and wheat samples (
n
= 95) harvested in Finland during 2013. Deoxynivalenol and its glucosylated form were the most abundant of the analytes, being detected in 93 and 81 % of the samples, respectively. Concentrations of deoxynivalenol were unusually high in 2013, especially in oats, with some cases exceeding the maximum legislative limits for unprocessed oats placed on the market for first-stage processing. All modified mycotoxins analysed were detected, and the natural occurrence of some of these compounds (e.g. zearalenone-16-glucoside and nivalenol-3-glucoside) in barley, oats and/or wheat was documented for the first time.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-015-8676-4</identifier><identifier>PMID: 25935671</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acetic acid ; Analysis ; Analytical Chemistry ; Barley ; Biochemistry ; Cereals ; Characterization and Evaluation of Materials ; Chemical properties ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Chromatography, Liquid - methods ; Deoxynivalenol ; deoxynivalenol-3-glucoside ; Edible Grain - chemistry ; Finland ; food contamination ; Food safety ; Food Science ; Fusarium ; Grains ; Hordeum vulgare ; HT-2 toxin ; Laboratory Medicine ; Life sciences ; Liquid chromatography ; Liquids ; Mass spectrometry ; Metabolism ; Metabolites ; Methods ; monitoring ; Monitoring/Environmental Analysis ; Mycotoxins ; national surveys ; nivalenol ; Oats ; quantitative analysis ; Research Paper ; reversed-phase liquid chromatography ; Sample preparation ; T-2 toxin ; tandem mass spectrometry ; Tandem Mass Spectrometry - methods ; Toxicity ; Toxins ; Trichothecenes ; Trichothecenes - analysis ; Triticum aestivum ; Wheat ; Zearalenone ; Zearalenone - analysis</subject><ispartof>Analytical and bioanalytical chemistry, 2015-06, Vol.407 (16), p.4745-4755</ispartof><rights>The Author(s) 2015</rights><rights>COPYRIGHT 2015 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c715t-3a2643339bd765b35d8f0dad85d55463120d572837908d3a541f9cdfa088fa003</citedby><cites>FETCH-LOGICAL-c715t-3a2643339bd765b35d8f0dad85d55463120d572837908d3a541f9cdfa088fa003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-015-8676-4$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-015-8676-4$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25935671$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nathanail, Alexis V.</creatorcontrib><creatorcontrib>Syvähuoko, Jenna</creatorcontrib><creatorcontrib>Malachová, Alexandra</creatorcontrib><creatorcontrib>Jestoi, Marika</creatorcontrib><creatorcontrib>Varga, Elisabeth</creatorcontrib><creatorcontrib>Michlmayr, Herbert</creatorcontrib><creatorcontrib>Adam, Gerhard</creatorcontrib><creatorcontrib>Sieviläinen, Elina</creatorcontrib><creatorcontrib>Berthiller, Franz</creatorcontrib><creatorcontrib>Peltonen, Kimmo</creatorcontrib><title>Simultaneous determination of major type A and B trichothecenes, zearalenone and certain modified metabolites in Finnish cereal grains with a novel liquid chromatography-tandem mass spectrometric method</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>A reliable and sensitive liquid chromatography-tandem mass spectrometric method was developed for the simultaneous quantitative determination in cereals of the
Fusarium
mycotoxins HT-2 toxin, T-2 toxin, deoxynivalenol, nivalenol and zearalenone, as well as the modified metabolites 3-acetyl-deoxynivalenol, α-zearalenol, β-zearalenol, deoxynivalenol-3-glucoside, HT-2-3-glucoside, nivalenol-3-glucoside, zearalenone-14-glucoside, zearalenone-14-sulphate, zearalenone-16-glucoside, α-zearalenol-14-glucoside and β-zearalenol-14-glucoside. The ‘dilute and shoot’ approach was used for sample preparation after extraction with acetonitrile:water:acetic acid (79:20:1,
v
/
v
/
v
). Separation was carried out using reversed-phase liquid chromatography, and detection was performed using tandem mass spectrometry in the selected reaction monitoring mode. The method was in-house validated according to performance characteristics, established in Commission Regulation EC No 401/2006 and Commission Decision EC No 657/2002, prior to its application in a nationwide survey for the analysis of barley, oat and wheat samples (
n
= 95) harvested in Finland during 2013. Deoxynivalenol and its glucosylated form were the most abundant of the analytes, being detected in 93 and 81 % of the samples, respectively. Concentrations of deoxynivalenol were unusually high in 2013, especially in oats, with some cases exceeding the maximum legislative limits for unprocessed oats placed on the market for first-stage processing. All modified mycotoxins analysed were detected, and the natural occurrence of some of these compounds (e.g. zearalenone-16-glucoside and nivalenol-3-glucoside) in barley, oats and/or wheat was documented for the first time.</description><subject>Acetic acid</subject><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Barley</subject><subject>Biochemistry</subject><subject>Cereals</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical properties</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Chromatography, Liquid - methods</subject><subject>Deoxynivalenol</subject><subject>deoxynivalenol-3-glucoside</subject><subject>Edible Grain - chemistry</subject><subject>Finland</subject><subject>food contamination</subject><subject>Food safety</subject><subject>Food Science</subject><subject>Fusarium</subject><subject>Grains</subject><subject>Hordeum vulgare</subject><subject>HT-2 toxin</subject><subject>Laboratory Medicine</subject><subject>Life sciences</subject><subject>Liquid chromatography</subject><subject>Liquids</subject><subject>Mass spectrometry</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Methods</subject><subject>monitoring</subject><subject>Monitoring/Environmental Analysis</subject><subject>Mycotoxins</subject><subject>national surveys</subject><subject>nivalenol</subject><subject>Oats</subject><subject>quantitative analysis</subject><subject>Research Paper</subject><subject>reversed-phase liquid chromatography</subject><subject>Sample preparation</subject><subject>T-2 toxin</subject><subject>tandem mass spectrometry</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Toxicity</subject><subject>Toxins</subject><subject>Trichothecenes</subject><subject>Trichothecenes - analysis</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><subject>Zearalenone</subject><subject>Zearalenone - analysis</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkt-K1DAUxoso7jr6AN5IwBsv7Jo0f9reCOPiqrDghXodMs3pNEubzCbpyviIPpWnzjqsgiiFtuT88uXkO19RPGX0jFFav0qUVkyVlMmyUbUqxb3ilCnWlJWS9P7xX1QnxaOUriiCDVMPi5NKtlyqmp0W3z-5aR6z8RDmRCxkiJPzJrvgSejJZK5CJHm_A7ImxlvyhuTouiHkATrwkF6Sb2CiGcEHDz-JDmI2zpMpWNc7sGSCbDZhdBkSwfUL571Lw8KBGck2IpzIV5cHYogPNzCS0V3PDpWGGCaTAyK7YV9ikxYmbCklknbQZazC0s1ywhDs4-JBb8YET26_q-LLxdvP5-_Ly4_vPpyvL8uuZjKX3KAjnPN2Y2slN1zapqfW2EZaKYXirKJW1lXD65Y2lhspWN92tje0afBF-ap4fdDdzZsJLNqQ0QC9i24yca-Dcfr3ineD3oYbLYRQshIo8OJWIIbrGVLWk0sdjONhCprVQlVtK7DLf6OsaZUQ9H9UKRMN43jFVfH8D_QqzNGjaUgtHsi2XgTPDtQWx6ud7wPepsMHp-A6HHfvcH0tuOSNrNQiyw4buhhSitAfPWFUL4HVh8BqzKFeAquXQ57dNfO441dCEagOQMKS30K80-tfVX8AYR36OA</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Nathanail, Alexis V.</creator><creator>Syvähuoko, Jenna</creator><creator>Malachová, Alexandra</creator><creator>Jestoi, Marika</creator><creator>Varga, Elisabeth</creator><creator>Michlmayr, Herbert</creator><creator>Adam, Gerhard</creator><creator>Sieviläinen, Elina</creator><creator>Berthiller, Franz</creator><creator>Peltonen, Kimmo</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7QH</scope><scope>7UA</scope><scope>M7N</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20150601</creationdate><title>Simultaneous determination of major type A and B trichothecenes, zearalenone and certain modified metabolites in Finnish cereal grains with a novel liquid chromatography-tandem mass spectrometric method</title><author>Nathanail, Alexis V. ; Syvähuoko, Jenna ; Malachová, Alexandra ; Jestoi, Marika ; Varga, Elisabeth ; Michlmayr, Herbert ; Adam, Gerhard ; Sieviläinen, Elina ; Berthiller, Franz ; Peltonen, Kimmo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c715t-3a2643339bd765b35d8f0dad85d55463120d572837908d3a541f9cdfa088fa003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acetic acid</topic><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Barley</topic><topic>Biochemistry</topic><topic>Cereals</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical properties</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography</topic><topic>Chromatography, Liquid - methods</topic><topic>Deoxynivalenol</topic><topic>deoxynivalenol-3-glucoside</topic><topic>Edible Grain - chemistry</topic><topic>Finland</topic><topic>food contamination</topic><topic>Food safety</topic><topic>Food Science</topic><topic>Fusarium</topic><topic>Grains</topic><topic>Hordeum vulgare</topic><topic>HT-2 toxin</topic><topic>Laboratory Medicine</topic><topic>Life sciences</topic><topic>Liquid chromatography</topic><topic>Liquids</topic><topic>Mass spectrometry</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Methods</topic><topic>monitoring</topic><topic>Monitoring/Environmental Analysis</topic><topic>Mycotoxins</topic><topic>national surveys</topic><topic>nivalenol</topic><topic>Oats</topic><topic>quantitative analysis</topic><topic>Research Paper</topic><topic>reversed-phase liquid chromatography</topic><topic>Sample preparation</topic><topic>T-2 toxin</topic><topic>tandem mass spectrometry</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Toxicity</topic><topic>Toxins</topic><topic>Trichothecenes</topic><topic>Trichothecenes - analysis</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><topic>Zearalenone</topic><topic>Zearalenone - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nathanail, Alexis V.</creatorcontrib><creatorcontrib>Syvähuoko, Jenna</creatorcontrib><creatorcontrib>Malachová, Alexandra</creatorcontrib><creatorcontrib>Jestoi, Marika</creatorcontrib><creatorcontrib>Varga, Elisabeth</creatorcontrib><creatorcontrib>Michlmayr, Herbert</creatorcontrib><creatorcontrib>Adam, Gerhard</creatorcontrib><creatorcontrib>Sieviläinen, Elina</creatorcontrib><creatorcontrib>Berthiller, Franz</creatorcontrib><creatorcontrib>Peltonen, Kimmo</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nathanail, Alexis V.</au><au>Syvähuoko, Jenna</au><au>Malachová, Alexandra</au><au>Jestoi, Marika</au><au>Varga, Elisabeth</au><au>Michlmayr, Herbert</au><au>Adam, Gerhard</au><au>Sieviläinen, Elina</au><au>Berthiller, Franz</au><au>Peltonen, Kimmo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous determination of major type A and B trichothecenes, zearalenone and certain modified metabolites in Finnish cereal grains with a novel liquid chromatography-tandem mass spectrometric method</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>407</volume><issue>16</issue><spage>4745</spage><epage>4755</epage><pages>4745-4755</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>A reliable and sensitive liquid chromatography-tandem mass spectrometric method was developed for the simultaneous quantitative determination in cereals of the
Fusarium
mycotoxins HT-2 toxin, T-2 toxin, deoxynivalenol, nivalenol and zearalenone, as well as the modified metabolites 3-acetyl-deoxynivalenol, α-zearalenol, β-zearalenol, deoxynivalenol-3-glucoside, HT-2-3-glucoside, nivalenol-3-glucoside, zearalenone-14-glucoside, zearalenone-14-sulphate, zearalenone-16-glucoside, α-zearalenol-14-glucoside and β-zearalenol-14-glucoside. The ‘dilute and shoot’ approach was used for sample preparation after extraction with acetonitrile:water:acetic acid (79:20:1,
v
/
v
/
v
). Separation was carried out using reversed-phase liquid chromatography, and detection was performed using tandem mass spectrometry in the selected reaction monitoring mode. The method was in-house validated according to performance characteristics, established in Commission Regulation EC No 401/2006 and Commission Decision EC No 657/2002, prior to its application in a nationwide survey for the analysis of barley, oat and wheat samples (
n
= 95) harvested in Finland during 2013. Deoxynivalenol and its glucosylated form were the most abundant of the analytes, being detected in 93 and 81 % of the samples, respectively. Concentrations of deoxynivalenol were unusually high in 2013, especially in oats, with some cases exceeding the maximum legislative limits for unprocessed oats placed on the market for first-stage processing. All modified mycotoxins analysed were detected, and the natural occurrence of some of these compounds (e.g. zearalenone-16-glucoside and nivalenol-3-glucoside) in barley, oats and/or wheat was documented for the first time.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25935671</pmid><doi>10.1007/s00216-015-8676-4</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2015-06, Vol.407 (16), p.4745-4755 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4446524 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Acetic acid Analysis Analytical Chemistry Barley Biochemistry Cereals Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Chromatography Chromatography, Liquid - methods Deoxynivalenol deoxynivalenol-3-glucoside Edible Grain - chemistry Finland food contamination Food safety Food Science Fusarium Grains Hordeum vulgare HT-2 toxin Laboratory Medicine Life sciences Liquid chromatography Liquids Mass spectrometry Metabolism Metabolites Methods monitoring Monitoring/Environmental Analysis Mycotoxins national surveys nivalenol Oats quantitative analysis Research Paper reversed-phase liquid chromatography Sample preparation T-2 toxin tandem mass spectrometry Tandem Mass Spectrometry - methods Toxicity Toxins Trichothecenes Trichothecenes - analysis Triticum aestivum Wheat Zearalenone Zearalenone - analysis |
| title | Simultaneous determination of major type A and B trichothecenes, zearalenone and certain modified metabolites in Finnish cereal grains with a novel liquid chromatography-tandem mass spectrometric method |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T04%3A30%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Simultaneous%20determination%20of%20major%20type%20A%20and%20B%20trichothecenes,%20zearalenone%20and%20certain%20modified%20metabolites%20in%20Finnish%20cereal%20grains%20with%20a%20novel%20liquid%20chromatography-tandem%20mass%20spectrometric%20method&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=Nathanail,%20Alexis%20V.&rft.date=2015-06-01&rft.volume=407&rft.issue=16&rft.spage=4745&rft.epage=4755&rft.pages=4745-4755&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-015-8676-4&rft_dat=%3Cgale_pubme%3EA435385266%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1754635974&rft_id=info:pmid/25935671&rft_galeid=A435385266&rfr_iscdi=true |