Bioluminescence-activated deep-tissue photodynamic therapy of cancer
Optical energy can trigger a variety of photochemical processes useful for therapies. Owing to the shallow penetration of light in tissues, however, the clinical applications of light-activated therapies have been limited. Bioluminescence resonant energy transfer (BRET) may provide a new way of indu...
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| Veröffentlicht in: | Theranostics 2015-01, Vol.5 (8), p.805-817 |
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| container_title | Theranostics |
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| creator | Kim, Yi Rang Kim, Seonghoon Choi, Jin Woo Choi, Sung Yong Lee, Sang-Hee Kim, Homin Hahn, Sei Kwang Koh, Gou Young Yun, Seok Hyun |
| description | Optical energy can trigger a variety of photochemical processes useful for therapies. Owing to the shallow penetration of light in tissues, however, the clinical applications of light-activated therapies have been limited. Bioluminescence resonant energy transfer (BRET) may provide a new way of inducing photochemical activation. Here, we show that efficient bioluminescence energy-induced photodynamic therapy (PDT) of macroscopic tumors and metastases in deep tissue. For monolayer cell culture in vitro incubated with Chlorin e6, BRET energy of about 1 nJ per cell generated as strong cytotoxicity as red laser light irradiation at 2.2 mW/cm(2) for 180 s. Regional delivery of bioluminescence agents via draining lymphatic vessels killed tumor cells spread to the sentinel and secondary lymph nodes, reduced distant metastases in the lung and improved animal survival. Our results show the promising potential of novel bioluminescence-activated PDT. |
| doi_str_mv | 10.7150/thno.11520 |
| format | Article |
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Owing to the shallow penetration of light in tissues, however, the clinical applications of light-activated therapies have been limited. Bioluminescence resonant energy transfer (BRET) may provide a new way of inducing photochemical activation. Here, we show that efficient bioluminescence energy-induced photodynamic therapy (PDT) of macroscopic tumors and metastases in deep tissue. For monolayer cell culture in vitro incubated with Chlorin e6, BRET energy of about 1 nJ per cell generated as strong cytotoxicity as red laser light irradiation at 2.2 mW/cm(2) for 180 s. Regional delivery of bioluminescence agents via draining lymphatic vessels killed tumor cells spread to the sentinel and secondary lymph nodes, reduced distant metastases in the lung and improved animal survival. Our results show the promising potential of novel bioluminescence-activated PDT.</description><identifier>ISSN: 1838-7640</identifier><identifier>EISSN: 1838-7640</identifier><identifier>DOI: 10.7150/thno.11520</identifier><identifier>PMID: 26000054</identifier><language>eng</language><publisher>Australia: Ivyspring International Publisher</publisher><subject>Animals ; Disease Models, Animal ; Humans ; Lasers ; Luminescence ; Mice, Inbred C57BL ; Neoplasms - drug therapy ; Photochemotherapy - methods ; Porphyrins - metabolism ; Radiation-Sensitizing Agents - metabolism ; Research Paper</subject><ispartof>Theranostics, 2015-01, Vol.5 (8), p.805-817</ispartof><rights>2015 Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-504d6d449162e18a3c7276a604e4ee4ff544f17b3858ecaf472bf0e95bb799263</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440439/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440439/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26000054$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Yi Rang</creatorcontrib><creatorcontrib>Kim, Seonghoon</creatorcontrib><creatorcontrib>Choi, Jin Woo</creatorcontrib><creatorcontrib>Choi, Sung Yong</creatorcontrib><creatorcontrib>Lee, Sang-Hee</creatorcontrib><creatorcontrib>Kim, Homin</creatorcontrib><creatorcontrib>Hahn, Sei Kwang</creatorcontrib><creatorcontrib>Koh, Gou Young</creatorcontrib><creatorcontrib>Yun, Seok Hyun</creatorcontrib><title>Bioluminescence-activated deep-tissue photodynamic therapy of cancer</title><title>Theranostics</title><addtitle>Theranostics</addtitle><description>Optical energy can trigger a variety of photochemical processes useful for therapies. Owing to the shallow penetration of light in tissues, however, the clinical applications of light-activated therapies have been limited. Bioluminescence resonant energy transfer (BRET) may provide a new way of inducing photochemical activation. Here, we show that efficient bioluminescence energy-induced photodynamic therapy (PDT) of macroscopic tumors and metastases in deep tissue. For monolayer cell culture in vitro incubated with Chlorin e6, BRET energy of about 1 nJ per cell generated as strong cytotoxicity as red laser light irradiation at 2.2 mW/cm(2) for 180 s. Regional delivery of bioluminescence agents via draining lymphatic vessels killed tumor cells spread to the sentinel and secondary lymph nodes, reduced distant metastases in the lung and improved animal survival. Our results show the promising potential of novel bioluminescence-activated PDT.</description><subject>Animals</subject><subject>Disease Models, Animal</subject><subject>Humans</subject><subject>Lasers</subject><subject>Luminescence</subject><subject>Mice, Inbred C57BL</subject><subject>Neoplasms - drug therapy</subject><subject>Photochemotherapy - methods</subject><subject>Porphyrins - metabolism</subject><subject>Radiation-Sensitizing Agents - metabolism</subject><subject>Research Paper</subject><issn>1838-7640</issn><issn>1838-7640</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUE1PwzAMjRCITWMXfgDqESF1JE3StBckGJ_SJC5wjtLUYUFtU5p00v49LYxp-GJbfn7PfgidE7wQhOPrsG7cghCe4CM0JRnNYpEyfHxQT9Dc-088BMNJTvJTNEnSseVsiu7vrKv62jbgNTQaYqWD3agAZVQCtHGw3vcQtWsXXLltVG11FNbQqXYbORNpNex0Z-jEqMrDfJdn6P3x4W35HK9en16Wt6tYU5GFmGNWpiVjOUkTIJmiWiQiVSlmwACYMZwxQ0RBM56BVoaJpDAYcl4UIs-TlM7QzS9v2xc1lMPBoVOVbDtbq24rnbLy_6Sxa_nhNpIxhhnNB4LLHUHnvnrwQdZ2-LuqVAOu95KkGeWCZvmodfUL1Z3zvgOzlyFYjs7L0Xn54_wAvjg8bA_985l-A-fcgDw</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Kim, Yi Rang</creator><creator>Kim, Seonghoon</creator><creator>Choi, Jin Woo</creator><creator>Choi, Sung Yong</creator><creator>Lee, Sang-Hee</creator><creator>Kim, Homin</creator><creator>Hahn, Sei Kwang</creator><creator>Koh, Gou Young</creator><creator>Yun, Seok Hyun</creator><general>Ivyspring International Publisher</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>Bioluminescence-activated deep-tissue photodynamic therapy of cancer</title><author>Kim, Yi Rang ; Kim, Seonghoon ; Choi, Jin Woo ; Choi, Sung Yong ; Lee, Sang-Hee ; Kim, Homin ; Hahn, Sei Kwang ; Koh, Gou Young ; Yun, Seok Hyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-504d6d449162e18a3c7276a604e4ee4ff544f17b3858ecaf472bf0e95bb799263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Disease Models, Animal</topic><topic>Humans</topic><topic>Lasers</topic><topic>Luminescence</topic><topic>Mice, Inbred C57BL</topic><topic>Neoplasms - drug therapy</topic><topic>Photochemotherapy - methods</topic><topic>Porphyrins - metabolism</topic><topic>Radiation-Sensitizing Agents - metabolism</topic><topic>Research Paper</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Yi Rang</creatorcontrib><creatorcontrib>Kim, Seonghoon</creatorcontrib><creatorcontrib>Choi, Jin Woo</creatorcontrib><creatorcontrib>Choi, Sung Yong</creatorcontrib><creatorcontrib>Lee, Sang-Hee</creatorcontrib><creatorcontrib>Kim, Homin</creatorcontrib><creatorcontrib>Hahn, Sei Kwang</creatorcontrib><creatorcontrib>Koh, Gou Young</creatorcontrib><creatorcontrib>Yun, Seok Hyun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Theranostics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Yi Rang</au><au>Kim, Seonghoon</au><au>Choi, Jin Woo</au><au>Choi, Sung Yong</au><au>Lee, Sang-Hee</au><au>Kim, Homin</au><au>Hahn, Sei Kwang</au><au>Koh, Gou Young</au><au>Yun, Seok Hyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioluminescence-activated deep-tissue photodynamic therapy of cancer</atitle><jtitle>Theranostics</jtitle><addtitle>Theranostics</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>5</volume><issue>8</issue><spage>805</spage><epage>817</epage><pages>805-817</pages><issn>1838-7640</issn><eissn>1838-7640</eissn><abstract>Optical energy can trigger a variety of photochemical processes useful for therapies. Owing to the shallow penetration of light in tissues, however, the clinical applications of light-activated therapies have been limited. Bioluminescence resonant energy transfer (BRET) may provide a new way of inducing photochemical activation. Here, we show that efficient bioluminescence energy-induced photodynamic therapy (PDT) of macroscopic tumors and metastases in deep tissue. For monolayer cell culture in vitro incubated with Chlorin e6, BRET energy of about 1 nJ per cell generated as strong cytotoxicity as red laser light irradiation at 2.2 mW/cm(2) for 180 s. Regional delivery of bioluminescence agents via draining lymphatic vessels killed tumor cells spread to the sentinel and secondary lymph nodes, reduced distant metastases in the lung and improved animal survival. Our results show the promising potential of novel bioluminescence-activated PDT.</abstract><cop>Australia</cop><pub>Ivyspring International Publisher</pub><pmid>26000054</pmid><doi>10.7150/thno.11520</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Disease Models, Animal Humans Lasers Luminescence Mice, Inbred C57BL Neoplasms - drug therapy Photochemotherapy - methods Porphyrins - metabolism Radiation-Sensitizing Agents - metabolism Research Paper |
| title | Bioluminescence-activated deep-tissue photodynamic therapy of cancer |
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