Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes
Genomic in situ hybridization (GISH) has been widely used to detect rye ( Secale cereale L.) chromosomes in wheat ( Triticum aestivum L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeli...
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| description | Genomic
in situ
hybridization (GISH) has been widely used to detect rye (
Secale cereale
L.) chromosomes in wheat (
Triticum aestivum
L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of chromosomes and probes. In this study, new oligonucleotide probes Oligo-1162, Oligo-pSc200 and Oligo-pSc250 were developed. The three new probes can be used for non-denaturing fluorescence
in situ
hybridization (ND-FISH) assays and replace genomic DNA of rye as a probe to discriminate rye chromosomes in wheat backgrounds. In addition, previously developed oligonucleotide probes Oligo-pSc119.2-1, Oligo-pSc119.2-2, Oligo-pTa535-1, Oligo-pTa535-2, Oligo-pTa71-2, Oligo-pAWRC.1 and Oligo-CCS1 can also be used for ND-FISH of wheat and rye. These probes have provided an easier, faster and more cost-effective method for the FISH analysis of wheat and hybrids derived from wheat × rye. |
| doi_str_mv | 10.1038/srep10552 |
| format | Article |
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in situ
hybridization (GISH) has been widely used to detect rye (
Secale cereale
L.) chromosomes in wheat (
Triticum aestivum
L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of chromosomes and probes. In this study, new oligonucleotide probes Oligo-1162, Oligo-pSc200 and Oligo-pSc250 were developed. The three new probes can be used for non-denaturing fluorescence
in situ
hybridization (ND-FISH) assays and replace genomic DNA of rye as a probe to discriminate rye chromosomes in wheat backgrounds. In addition, previously developed oligonucleotide probes Oligo-pSc119.2-1, Oligo-pSc119.2-2, Oligo-pTa535-1, Oligo-pTa535-2, Oligo-pTa71-2, Oligo-pAWRC.1 and Oligo-CCS1 can also be used for ND-FISH of wheat and rye. These probes have provided an easier, faster and more cost-effective method for the FISH analysis of wheat and hybrids derived from wheat × rye.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep10552</identifier><identifier>PMID: 25994088</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 14 ; 38/32 ; 631/1647/1407/1405 ; 631/449/711 ; Chromosomes ; Chromosomes, Plant - genetics ; Chromosomes, Plant - metabolism ; Deoxyribonucleic acid ; DNA ; DNA probes ; Fluorescence in situ hybridization ; Fluorescent indicators ; Genomic in situ hybridization ; Genomics ; Humanities and Social Sciences ; Hybridization ; Hybrids ; In Situ Hybridization, Fluorescence ; Microscopy, Fluorescence ; multidisciplinary ; Oligonucleotide Probes - genetics ; Oligonucleotide Probes - metabolism ; Oligonucleotides ; Polyploidy ; Probes ; Science ; Secale - genetics ; Triticum - genetics ; Triticum aestivum ; Wheat</subject><ispartof>Scientific reports, 2015-05, Vol.5 (1), p.10552-10552, Article 10552</ispartof><rights>The Author(s) 2015</rights><rights>Copyright Nature Publishing Group May 2015</rights><rights>Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-e93dd61d1350a7f65a5bd2459f5c4a545fd79c3dec3ec625dabc363050e52f403</citedby><cites>FETCH-LOGICAL-c504t-e93dd61d1350a7f65a5bd2459f5c4a545fd79c3dec3ec625dabc363050e52f403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440213/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440213/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25994088$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fu, Shulan</creatorcontrib><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Wang, Yangyang</creatorcontrib><creatorcontrib>Li, Meng</creatorcontrib><creatorcontrib>Yang, Zujun</creatorcontrib><creatorcontrib>Qiu, Ling</creatorcontrib><creatorcontrib>Yan, Benju</creatorcontrib><creatorcontrib>Ren, Zhenglong</creatorcontrib><creatorcontrib>Tang, Zongxiang</creatorcontrib><title>Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Genomic
in situ
hybridization (GISH) has been widely used to detect rye (
Secale cereale
L.) chromosomes in wheat (
Triticum aestivum
L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of chromosomes and probes. In this study, new oligonucleotide probes Oligo-1162, Oligo-pSc200 and Oligo-pSc250 were developed. The three new probes can be used for non-denaturing fluorescence
in situ
hybridization (ND-FISH) assays and replace genomic DNA of rye as a probe to discriminate rye chromosomes in wheat backgrounds. In addition, previously developed oligonucleotide probes Oligo-pSc119.2-1, Oligo-pSc119.2-2, Oligo-pTa535-1, Oligo-pTa535-2, Oligo-pTa71-2, Oligo-pAWRC.1 and Oligo-CCS1 can also be used for ND-FISH of wheat and rye. These probes have provided an easier, faster and more cost-effective method for the FISH analysis of wheat and hybrids derived from wheat × rye.</description><subject>13</subject><subject>14</subject><subject>38/32</subject><subject>631/1647/1407/1405</subject><subject>631/449/711</subject><subject>Chromosomes</subject><subject>Chromosomes, Plant - genetics</subject><subject>Chromosomes, Plant - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA probes</subject><subject>Fluorescence in situ hybridization</subject><subject>Fluorescent indicators</subject><subject>Genomic in situ hybridization</subject><subject>Genomics</subject><subject>Humanities and Social Sciences</subject><subject>Hybridization</subject><subject>Hybrids</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Microscopy, Fluorescence</subject><subject>multidisciplinary</subject><subject>Oligonucleotide Probes - genetics</subject><subject>Oligonucleotide Probes - metabolism</subject><subject>Oligonucleotides</subject><subject>Polyploidy</subject><subject>Probes</subject><subject>Science</subject><subject>Secale - genetics</subject><subject>Triticum - genetics</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNplkVtrGzEQhUVpaUKah_6BIuhLW9hUt1lLL4XgNo0hJL3SRyFLs_aG3ZUj7Rb876vixDjtvMzAfJw5zCHkJWdnnEn9PifccAYgnpBjwRRUQgrx9GA-Iqc537JSIIzi5jk5EmCMYlofk683XbuKw-Q7jGMbkH5JcYmZNjHR64_VxeL7JT0fXLfNbaZjpIuAw9g2W_pti9QNgf5aoxvpfJ1iH3PsMb8gzxrXZTy97yfk58WnH_PL6urm82J-flV5YGqs0MgQah64BOZmTQ0OlkEoMA145UBBE2bGy4Beoq8FBLf0spYMGIJoFJMn5MNOdzMtewy--Equs5vU9i5tbXStfbwZ2rVdxd9WKcUEl0Xgzb1AincT5tH2bfbYdW7AOGXLay20rme6Lujrf9DbOKXylkJpYwA0yFmh3u4on2IusTR7M5zZv1nZfVaFfXXofk8-JFOAdzsgl9WwwnRw8j-1P8zInWw</recordid><startdate>20150521</startdate><enddate>20150521</enddate><creator>Fu, Shulan</creator><creator>Chen, Lei</creator><creator>Wang, Yangyang</creator><creator>Li, Meng</creator><creator>Yang, Zujun</creator><creator>Qiu, Ling</creator><creator>Yan, Benju</creator><creator>Ren, Zhenglong</creator><creator>Tang, Zongxiang</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150521</creationdate><title>Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes</title><author>Fu, Shulan ; Chen, Lei ; Wang, Yangyang ; Li, Meng ; Yang, Zujun ; Qiu, Ling ; Yan, Benju ; Ren, Zhenglong ; Tang, Zongxiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-e93dd61d1350a7f65a5bd2459f5c4a545fd79c3dec3ec625dabc363050e52f403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>13</topic><topic>14</topic><topic>38/32</topic><topic>631/1647/1407/1405</topic><topic>631/449/711</topic><topic>Chromosomes</topic><topic>Chromosomes, Plant - genetics</topic><topic>Chromosomes, Plant - metabolism</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA probes</topic><topic>Fluorescence in situ hybridization</topic><topic>Fluorescent indicators</topic><topic>Genomic in situ hybridization</topic><topic>Genomics</topic><topic>Humanities and Social Sciences</topic><topic>Hybridization</topic><topic>Hybrids</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Microscopy, Fluorescence</topic><topic>multidisciplinary</topic><topic>Oligonucleotide Probes - genetics</topic><topic>Oligonucleotide Probes - metabolism</topic><topic>Oligonucleotides</topic><topic>Polyploidy</topic><topic>Probes</topic><topic>Science</topic><topic>Secale - genetics</topic><topic>Triticum - genetics</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fu, Shulan</creatorcontrib><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Wang, Yangyang</creatorcontrib><creatorcontrib>Li, Meng</creatorcontrib><creatorcontrib>Yang, Zujun</creatorcontrib><creatorcontrib>Qiu, Ling</creatorcontrib><creatorcontrib>Yan, Benju</creatorcontrib><creatorcontrib>Ren, Zhenglong</creatorcontrib><creatorcontrib>Tang, Zongxiang</creatorcontrib><collection>Springer_OA刊</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Complete (ProQuest Database)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Shulan</au><au>Chen, Lei</au><au>Wang, Yangyang</au><au>Li, Meng</au><au>Yang, Zujun</au><au>Qiu, Ling</au><au>Yan, Benju</au><au>Ren, Zhenglong</au><au>Tang, Zongxiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2015-05-21</date><risdate>2015</risdate><volume>5</volume><issue>1</issue><spage>10552</spage><epage>10552</epage><pages>10552-10552</pages><artnum>10552</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Genomic
in situ
hybridization (GISH) has been widely used to detect rye (
Secale cereale
L.) chromosomes in wheat (
Triticum aestivum
L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of chromosomes and probes. In this study, new oligonucleotide probes Oligo-1162, Oligo-pSc200 and Oligo-pSc250 were developed. The three new probes can be used for non-denaturing fluorescence
in situ
hybridization (ND-FISH) assays and replace genomic DNA of rye as a probe to discriminate rye chromosomes in wheat backgrounds. In addition, previously developed oligonucleotide probes Oligo-pSc119.2-1, Oligo-pSc119.2-2, Oligo-pTa535-1, Oligo-pTa535-2, Oligo-pTa71-2, Oligo-pAWRC.1 and Oligo-CCS1 can also be used for ND-FISH of wheat and rye. These probes have provided an easier, faster and more cost-effective method for the FISH analysis of wheat and hybrids derived from wheat × rye.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>25994088</pmid><doi>10.1038/srep10552</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Nature Free; Springer_OA刊; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 13 14 38/32 631/1647/1407/1405 631/449/711 Chromosomes Chromosomes, Plant - genetics Chromosomes, Plant - metabolism Deoxyribonucleic acid DNA DNA probes Fluorescence in situ hybridization Fluorescent indicators Genomic in situ hybridization Genomics Humanities and Social Sciences Hybridization Hybrids In Situ Hybridization, Fluorescence Microscopy, Fluorescence multidisciplinary Oligonucleotide Probes - genetics Oligonucleotide Probes - metabolism Oligonucleotides Polyploidy Probes Science Secale - genetics Triticum - genetics Triticum aestivum Wheat |
| title | Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes |
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