A new mouse model for wound healing in hemophilia A
To establish a new mouse model for wound healing studies on hemophilia A. Total 54 male mice with different genotypes including wild-type nude mice, heterozygous mice (FVIII-/-/Nu) and FVIII deficient mice (FVIII-/-) were generated and verified by PCR. Mice were subjected to wound healing research b...
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| Veröffentlicht in: | International journal of clinical and experimental pathology 2015-01, Vol.8 (3), p.3015-3021 |
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| creator | Gao, Guangwei Mashausi, Dhahiri Saidi Negi, Hema Li, Dongsheng Li, Dawei |
| description | To establish a new mouse model for wound healing studies on hemophilia A.
Total 54 male mice with different genotypes including wild-type nude mice, heterozygous mice (FVIII-/-/Nu) and FVIII deficient mice (FVIII-/-) were generated and verified by PCR. Mice were subjected to wound healing research by making a 5 mm-thickness wound on mice skin and applying recombinant human epidermal growth factor (EGF, 10 μg/g) ointment, FVIII ointment (30 IU) or the ointment base to heal the wounds. Furthermore, keratinocytes were isolated from these newborn mice and subjected to migration assay by stimulation of EGF (ng/ml), insulin (10 μM) or vehicle.
A new hemophilic mouse model (FVIII-/-/Nu) was constructed successfully after genotyping verified by PCR. Compared to FVIII-/- mice, FVIII-/-/Nu and Nu mice showed greater degree of wound contraction and loss of the crust. Topical treatment with EGF exhibited faster wound healing than FVIII and ointment base. Insulin treatment showed more increased migration distance than treated with EGF or vehicle. FVIII-/-/Nu mice showed greater migration than FVIII-/- and Nu mice.
A new mouse model (FVIII-/-/Nu) for wound healing in hemophilia A was constructed, and topical treatment of insulin may be a better therapy than EGF for healing wounds in hemophilia A. |
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Total 54 male mice with different genotypes including wild-type nude mice, heterozygous mice (FVIII-/-/Nu) and FVIII deficient mice (FVIII-/-) were generated and verified by PCR. Mice were subjected to wound healing research by making a 5 mm-thickness wound on mice skin and applying recombinant human epidermal growth factor (EGF, 10 μg/g) ointment, FVIII ointment (30 IU) or the ointment base to heal the wounds. Furthermore, keratinocytes were isolated from these newborn mice and subjected to migration assay by stimulation of EGF (ng/ml), insulin (10 μM) or vehicle.
A new hemophilic mouse model (FVIII-/-/Nu) was constructed successfully after genotyping verified by PCR. Compared to FVIII-/- mice, FVIII-/-/Nu and Nu mice showed greater degree of wound contraction and loss of the crust. Topical treatment with EGF exhibited faster wound healing than FVIII and ointment base. Insulin treatment showed more increased migration distance than treated with EGF or vehicle. FVIII-/-/Nu mice showed greater migration than FVIII-/- and Nu mice.
A new mouse model (FVIII-/-/Nu) for wound healing in hemophilia A was constructed, and topical treatment of insulin may be a better therapy than EGF for healing wounds in hemophilia A.</description><identifier>EISSN: 1936-2625</identifier><identifier>PMID: 26045812</identifier><language>eng</language><publisher>United States: e-Century Publishing Corporation</publisher><subject>Animals ; Disease Models, Animal ; Hemophilia A ; Mice ; Mice, Mutant Strains ; Original ; Polymerase Chain Reaction ; Wound Healing</subject><ispartof>International journal of clinical and experimental pathology, 2015-01, Vol.8 (3), p.3015-3021</ispartof><rights>IJCEP Copyright © 2015 2015</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440121/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440121/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26045812$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Guangwei</creatorcontrib><creatorcontrib>Mashausi, Dhahiri Saidi</creatorcontrib><creatorcontrib>Negi, Hema</creatorcontrib><creatorcontrib>Li, Dongsheng</creatorcontrib><creatorcontrib>Li, Dawei</creatorcontrib><title>A new mouse model for wound healing in hemophilia A</title><title>International journal of clinical and experimental pathology</title><addtitle>Int J Clin Exp Pathol</addtitle><description>To establish a new mouse model for wound healing studies on hemophilia A.
Total 54 male mice with different genotypes including wild-type nude mice, heterozygous mice (FVIII-/-/Nu) and FVIII deficient mice (FVIII-/-) were generated and verified by PCR. Mice were subjected to wound healing research by making a 5 mm-thickness wound on mice skin and applying recombinant human epidermal growth factor (EGF, 10 μg/g) ointment, FVIII ointment (30 IU) or the ointment base to heal the wounds. Furthermore, keratinocytes were isolated from these newborn mice and subjected to migration assay by stimulation of EGF (ng/ml), insulin (10 μM) or vehicle.
A new hemophilic mouse model (FVIII-/-/Nu) was constructed successfully after genotyping verified by PCR. Compared to FVIII-/- mice, FVIII-/-/Nu and Nu mice showed greater degree of wound contraction and loss of the crust. Topical treatment with EGF exhibited faster wound healing than FVIII and ointment base. Insulin treatment showed more increased migration distance than treated with EGF or vehicle. FVIII-/-/Nu mice showed greater migration than FVIII-/- and Nu mice.
A new mouse model (FVIII-/-/Nu) for wound healing in hemophilia A was constructed, and topical treatment of insulin may be a better therapy than EGF for healing wounds in hemophilia A.</description><subject>Animals</subject><subject>Disease Models, Animal</subject><subject>Hemophilia A</subject><subject>Mice</subject><subject>Mice, Mutant Strains</subject><subject>Original</subject><subject>Polymerase Chain Reaction</subject><subject>Wound Healing</subject><issn>1936-2625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEtLAzEUhYMgtlb_gmTpZiDvTDZCKT4KBTe6DpnMnTaSScZ5WPz3DlhFN-deOJfvcM8ZWlLDVcEUkwt0OQxvhCjKBLlAC6aIkCVlS8TXOMERt3kaYNYaIm5yj495SjU-gIsh7XFI89rm7hBicHh9hc4bFwe4Ps0Ven24f9k8Fbvnx-1mvSs6ztVYQGWMr0vPiDa15GWjWeOdpFQ2WnLutAEHqjLKeMV844n0XHgmJZOCm4rxFbr75nZT1ULtIY29i7brQ-v6T5tdsP-dFA52nz-sEIJQRmfA7QnQ5_cJhtG2YfAQo0swP2ypKpWgutR6Pr35m_Ub8tMU_wLiLWNk</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Gao, Guangwei</creator><creator>Mashausi, Dhahiri Saidi</creator><creator>Negi, Hema</creator><creator>Li, Dongsheng</creator><creator>Li, Dawei</creator><general>e-Century Publishing Corporation</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>A new mouse model for wound healing in hemophilia A</title><author>Gao, Guangwei ; Mashausi, Dhahiri Saidi ; Negi, Hema ; Li, Dongsheng ; Li, Dawei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p336t-eb99cd8c2079d538f72fca5115f7533a79eae6b969c62cfc05c34c25525439b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Disease Models, Animal</topic><topic>Hemophilia A</topic><topic>Mice</topic><topic>Mice, Mutant Strains</topic><topic>Original</topic><topic>Polymerase Chain Reaction</topic><topic>Wound Healing</topic><toplevel>online_resources</toplevel><creatorcontrib>Gao, Guangwei</creatorcontrib><creatorcontrib>Mashausi, Dhahiri Saidi</creatorcontrib><creatorcontrib>Negi, Hema</creatorcontrib><creatorcontrib>Li, Dongsheng</creatorcontrib><creatorcontrib>Li, Dawei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of clinical and experimental pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Guangwei</au><au>Mashausi, Dhahiri Saidi</au><au>Negi, Hema</au><au>Li, Dongsheng</au><au>Li, Dawei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new mouse model for wound healing in hemophilia A</atitle><jtitle>International journal of clinical and experimental pathology</jtitle><addtitle>Int J Clin Exp Pathol</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>8</volume><issue>3</issue><spage>3015</spage><epage>3021</epage><pages>3015-3021</pages><eissn>1936-2625</eissn><abstract>To establish a new mouse model for wound healing studies on hemophilia A.
Total 54 male mice with different genotypes including wild-type nude mice, heterozygous mice (FVIII-/-/Nu) and FVIII deficient mice (FVIII-/-) were generated and verified by PCR. Mice were subjected to wound healing research by making a 5 mm-thickness wound on mice skin and applying recombinant human epidermal growth factor (EGF, 10 μg/g) ointment, FVIII ointment (30 IU) or the ointment base to heal the wounds. Furthermore, keratinocytes were isolated from these newborn mice and subjected to migration assay by stimulation of EGF (ng/ml), insulin (10 μM) or vehicle.
A new hemophilic mouse model (FVIII-/-/Nu) was constructed successfully after genotyping verified by PCR. Compared to FVIII-/- mice, FVIII-/-/Nu and Nu mice showed greater degree of wound contraction and loss of the crust. Topical treatment with EGF exhibited faster wound healing than FVIII and ointment base. Insulin treatment showed more increased migration distance than treated with EGF or vehicle. FVIII-/-/Nu mice showed greater migration than FVIII-/- and Nu mice.
A new mouse model (FVIII-/-/Nu) for wound healing in hemophilia A was constructed, and topical treatment of insulin may be a better therapy than EGF for healing wounds in hemophilia A.</abstract><cop>United States</cop><pub>e-Century Publishing Corporation</pub><pmid>26045812</pmid><tpages>7</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Disease Models, Animal Hemophilia A Mice Mice, Mutant Strains Original Polymerase Chain Reaction Wound Healing |
| title | A new mouse model for wound healing in hemophilia A |
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