Protein Purification Using PDZ Affinity Chromatography

PDZ domains function in nature as protein‐binding domains within scaffold and membrane‐associated proteins. They comprise approximately 90 residues and undergo specific, high‐affinity interactions with complementary C‐terminal peptide sequences, other PDZ domains, and/or phospholipids. We have previ...

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Veröffentlicht in:	Current Protocols in Protein Science 2015-04, Vol.80 (1), p.9.10.1-9.10.37
Hauptverfasser:	Walkup, Ward G., Kennedy, Mary B.
Format:	Artikel
Sprache:	eng
Schlagworte:	affinity chromatography Affinity Labels affinity tag Amino Acid Sequence Animals Chromatography, Affinity - methods ligand matrices Molecular Sequence Data PDZ domain PDZ Domains peptide protein purification Proteins - isolation & purification Recombinant Fusion Proteins - isolation & purification
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