Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP

The crystal structures of prostaglandin F synthase (PGF) from both Leishmania major and Trypanosoma cruzi with and without their cofactor NADP have been determined to resolutions of 2.6 Å for T. cruzi PGF, 1.25 Å for T. cruzi PGF with NADP, 1.6 Å for L. major PGF and 1.8 Å for L. major PGF with NADP...

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Veröffentlicht in:	Acta crystallographica. Section F, Structural biology communications Structural biology communications, 2015-05, Vol.71 (5), p.609-614
Hauptverfasser:	Moen, Spencer O., Fairman, James W., Barnes, Steve R., Sullivan, Amy, Nakazawa-Hewitt, Stephen, Van Voorhis, Wesley C., Staker, Bart L., Lorimer, Donald D., Myler, Peter J., Edwards, Thomas E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Chagas disease Crystallization Humans Hydroxyprostaglandin Dehydrogenases - chemistry Hydroxyprostaglandin Dehydrogenases - genetics infectious diseases Leishmania major - chemistry Leishmania major - genetics leishmaniasis Molecular Parasitology Molecular Sequence Data NADP - chemistry NADP - genetics prostaglandin Protein Structure, Secondary SSGCID Trypanosoma cruzi - chemistry Trypanosoma cruzi - genetics
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Zusammenfassung:	The crystal structures of prostaglandin F synthase (PGF) from both Leishmania major and Trypanosoma cruzi with and without their cofactor NADP have been determined to resolutions of 2.6 Å for T. cruzi PGF, 1.25 Å for T. cruzi PGF with NADP, 1.6 Å for L. major PGF and 1.8 Å for L. major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% (Rfree of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target.
ISSN:	2053-230X 2053-230X
DOI:	10.1107/S2053230X15006883