Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury
Peripheral nerve injury (PNI) is a common disease, which results in a partial or total loss of motor, sensory and autonomic functions, leading to a decrease in quality of life. Schwann cells play a vital role in maintaining the peripheral nervous system and in injury and repair. Using immunohistoche...
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| Veröffentlicht in: | Purinergic signalling 2015-06, Vol.11 (2), p.203-213 |
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| creator | Song, Xian-min Xu, Xiao-hui Zhu, Jiao Guo, Zhili Li, Jian He, Cheng Burnstock, Geoffrey Yuan, Hongbin Xiang, Zhenghua |
| description | Peripheral nerve injury (PNI) is a common disease, which results in a partial or total loss of motor, sensory and autonomic functions, leading to a decrease in quality of life. Schwann cells play a vital role in maintaining the peripheral nervous system and in injury and repair. Using immunohistochemistry, Western blot, calcium assay and bromodeoxyuridine (BrdU) proliferation assay, the present study clearly demonstrated that P2X7 receptors (R) were expressed in myelinating and non-myelinating Schwann cells in longitudinal sections of sciatic nerves. After sciatic nerve injury (SNI), P2X7R expression in Schwann cells of injured sciatic nerves was significantly up-regulated during the early days of SNI. Double immunofluorescence of proliferating cell nuclear antigen (PCNA) and P2X7R implied that P2X7R may be involved in proliferation of Schwann cells. Further experiments on primary cultures of Schwann cells showed that P2X7R are functionally expressed in Schwann cells of rat sciatic nerves; ATP via P2X7R can promote Schwann cell proliferation, possibly via the MAPK/ERK intracellular signalling pathway. Other possible roles of P2X7R on Schwann cells are discussed. |
| doi_str_mv | 10.1007/s11302-015-9445-8 |
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Schwann cells play a vital role in maintaining the peripheral nervous system and in injury and repair. Using immunohistochemistry, Western blot, calcium assay and bromodeoxyuridine (BrdU) proliferation assay, the present study clearly demonstrated that P2X7 receptors (R) were expressed in myelinating and non-myelinating Schwann cells in longitudinal sections of sciatic nerves. After sciatic nerve injury (SNI), P2X7R expression in Schwann cells of injured sciatic nerves was significantly up-regulated during the early days of SNI. Double immunofluorescence of proliferating cell nuclear antigen (PCNA) and P2X7R implied that P2X7R may be involved in proliferation of Schwann cells. Further experiments on primary cultures of Schwann cells showed that P2X7R are functionally expressed in Schwann cells of rat sciatic nerves; ATP via P2X7R can promote Schwann cell proliferation, possibly via the MAPK/ERK intracellular signalling pathway. Other possible roles of P2X7R on Schwann cells are discussed.</description><identifier>ISSN: 1573-9538</identifier><identifier>EISSN: 1573-9546</identifier><identifier>DOI: 10.1007/s11302-015-9445-8</identifier><identifier>PMID: 25682129</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; Cell Proliferation - physiology ; Ganglia, Spinal - metabolism ; Human Physiology ; Male ; Nerve Regeneration - physiology ; Neurosciences ; Original ; Original Article ; Pharmacology/Toxicology ; Rats, Sprague-Dawley ; Receptors, Purinergic P2X7 - metabolism ; Schwann Cells - cytology ; Schwann Cells - metabolism ; Sciatic Nerve - injuries ; Sciatic Neuropathy - pathology ; Up-Regulation - physiology</subject><ispartof>Purinergic signalling, 2015-06, Vol.11 (2), p.203-213</ispartof><rights>Springer Science+Business Media Dordrecht 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c545t-7f1c586ccce29191570fa4fa96d1128a96fbcb7402f6feb0f3ec4b7fd8289bdb3</citedby><cites>FETCH-LOGICAL-c545t-7f1c586ccce29191570fa4fa96d1128a96fbcb7402f6feb0f3ec4b7fd8289bdb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425717/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425717/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25682129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Xian-min</creatorcontrib><creatorcontrib>Xu, Xiao-hui</creatorcontrib><creatorcontrib>Zhu, Jiao</creatorcontrib><creatorcontrib>Guo, Zhili</creatorcontrib><creatorcontrib>Li, Jian</creatorcontrib><creatorcontrib>He, Cheng</creatorcontrib><creatorcontrib>Burnstock, Geoffrey</creatorcontrib><creatorcontrib>Yuan, Hongbin</creatorcontrib><creatorcontrib>Xiang, Zhenghua</creatorcontrib><title>Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury</title><title>Purinergic signalling</title><addtitle>Purinergic Signalling</addtitle><addtitle>Purinergic Signal</addtitle><description>Peripheral nerve injury (PNI) is a common disease, which results in a partial or total loss of motor, sensory and autonomic functions, leading to a decrease in quality of life. Schwann cells play a vital role in maintaining the peripheral nervous system and in injury and repair. Using immunohistochemistry, Western blot, calcium assay and bromodeoxyuridine (BrdU) proliferation assay, the present study clearly demonstrated that P2X7 receptors (R) were expressed in myelinating and non-myelinating Schwann cells in longitudinal sections of sciatic nerves. After sciatic nerve injury (SNI), P2X7R expression in Schwann cells of injured sciatic nerves was significantly up-regulated during the early days of SNI. Double immunofluorescence of proliferating cell nuclear antigen (PCNA) and P2X7R implied that P2X7R may be involved in proliferation of Schwann cells. Further experiments on primary cultures of Schwann cells showed that P2X7R are functionally expressed in Schwann cells of rat sciatic nerves; ATP via P2X7R can promote Schwann cell proliferation, possibly via the MAPK/ERK intracellular signalling pathway. Other possible roles of P2X7R on Schwann cells are discussed.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Cell Proliferation - physiology</subject><subject>Ganglia, Spinal - metabolism</subject><subject>Human Physiology</subject><subject>Male</subject><subject>Nerve Regeneration - physiology</subject><subject>Neurosciences</subject><subject>Original</subject><subject>Original Article</subject><subject>Pharmacology/Toxicology</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Purinergic P2X7 - metabolism</subject><subject>Schwann Cells - cytology</subject><subject>Schwann Cells - metabolism</subject><subject>Sciatic Nerve - injuries</subject><subject>Sciatic Neuropathy - pathology</subject><subject>Up-Regulation - physiology</subject><issn>1573-9538</issn><issn>1573-9546</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtLJDEUhYOM-P4BboYsZ1OapJJKshkYZHyAoKCCu5BK37TVVCdlUuXgvzdNa6ObwdUNnO8ezs1B6JiSE0qIPM2U1oRVhIpKcy4qtYX2qJB1pQVvfmzetdpF-zkvCBGc1XoH7TLRKEaZ3kPuYagSzKfejl0MOHp8yx4lTuBgGGPKeAmzrmhhjocU-85D2pB37umfDQE76PuMrR8h4exWtMMB0gvgLiym9HqItr3tMxy9zwP0cP73_uyyur65uDr7c105wcVYSU-dUI1zDpimuoQn3nJvdTOjlKkyfetayQnzjYeW-Bocb6WfKaZ0O2vrA_R77TtMbYntIIzJ9mZI3dKmVxNtZ74qoXsy8_hiOGdCUlkMfr0bpPg8QR7Nssur62yAOGVDG821Ekzxb6CKUKl1IwpK16hLMecEfpOIErPq0ax7NKVHs-rRqLLz8_Mpm42P4grA1kAuUphDMos4pVC-9z-ub_wzqzk</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Song, Xian-min</creator><creator>Xu, Xiao-hui</creator><creator>Zhu, Jiao</creator><creator>Guo, Zhili</creator><creator>Li, Jian</creator><creator>He, Cheng</creator><creator>Burnstock, Geoffrey</creator><creator>Yuan, Hongbin</creator><creator>Xiang, Zhenghua</creator><general>Springer Netherlands</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope><scope>7TM</scope><scope>5PM</scope></search><sort><creationdate>20150601</creationdate><title>Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury</title><author>Song, Xian-min ; Xu, Xiao-hui ; Zhu, Jiao ; Guo, Zhili ; Li, Jian ; He, Cheng ; Burnstock, Geoffrey ; Yuan, Hongbin ; Xiang, Zhenghua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c545t-7f1c586ccce29191570fa4fa96d1128a96fbcb7402f6feb0f3ec4b7fd8289bdb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>Cell Proliferation - physiology</topic><topic>Ganglia, Spinal - metabolism</topic><topic>Human Physiology</topic><topic>Male</topic><topic>Nerve Regeneration - physiology</topic><topic>Neurosciences</topic><topic>Original</topic><topic>Original Article</topic><topic>Pharmacology/Toxicology</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Purinergic P2X7 - metabolism</topic><topic>Schwann Cells - cytology</topic><topic>Schwann Cells - metabolism</topic><topic>Sciatic Nerve - injuries</topic><topic>Sciatic Neuropathy - pathology</topic><topic>Up-Regulation - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Xian-min</creatorcontrib><creatorcontrib>Xu, Xiao-hui</creatorcontrib><creatorcontrib>Zhu, Jiao</creatorcontrib><creatorcontrib>Guo, Zhili</creatorcontrib><creatorcontrib>Li, Jian</creatorcontrib><creatorcontrib>He, Cheng</creatorcontrib><creatorcontrib>Burnstock, Geoffrey</creatorcontrib><creatorcontrib>Yuan, Hongbin</creatorcontrib><creatorcontrib>Xiang, Zhenghua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Purinergic signalling</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Xian-min</au><au>Xu, Xiao-hui</au><au>Zhu, Jiao</au><au>Guo, Zhili</au><au>Li, Jian</au><au>He, Cheng</au><au>Burnstock, Geoffrey</au><au>Yuan, Hongbin</au><au>Xiang, Zhenghua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury</atitle><jtitle>Purinergic signalling</jtitle><stitle>Purinergic Signalling</stitle><addtitle>Purinergic Signal</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>11</volume><issue>2</issue><spage>203</spage><epage>213</epage><pages>203-213</pages><issn>1573-9538</issn><eissn>1573-9546</eissn><abstract>Peripheral nerve injury (PNI) is a common disease, which results in a partial or total loss of motor, sensory and autonomic functions, leading to a decrease in quality of life. Schwann cells play a vital role in maintaining the peripheral nervous system and in injury and repair. Using immunohistochemistry, Western blot, calcium assay and bromodeoxyuridine (BrdU) proliferation assay, the present study clearly demonstrated that P2X7 receptors (R) were expressed in myelinating and non-myelinating Schwann cells in longitudinal sections of sciatic nerves. After sciatic nerve injury (SNI), P2X7R expression in Schwann cells of injured sciatic nerves was significantly up-regulated during the early days of SNI. Double immunofluorescence of proliferating cell nuclear antigen (PCNA) and P2X7R implied that P2X7R may be involved in proliferation of Schwann cells. Further experiments on primary cultures of Schwann cells showed that P2X7R are functionally expressed in Schwann cells of rat sciatic nerves; ATP via P2X7R can promote Schwann cell proliferation, possibly via the MAPK/ERK intracellular signalling pathway. 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| subjects | Animals Biomedical and Life Sciences Biomedicine Cancer Research Cell Proliferation - physiology Ganglia, Spinal - metabolism Human Physiology Male Nerve Regeneration - physiology Neurosciences Original Original Article Pharmacology/Toxicology Rats, Sprague-Dawley Receptors, Purinergic P2X7 - metabolism Schwann Cells - cytology Schwann Cells - metabolism Sciatic Nerve - injuries Sciatic Neuropathy - pathology Up-Regulation - physiology |
| title | Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury |
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