Exportin‐5 mediates nuclear export of SRP RNA in vertebrates
The signal recognition particle is a ribonucleoprotein complex that is essential for the translocation of nascent proteins into the endoplasmic reticulum. It has been shown that the RNA component (SRP RNA) is exported from the nucleus by CRM1 in the budding yeast. However, how SRP RNA is exported in...
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| Veröffentlicht in: | Genes to cells : devoted to molecular & cellular mechanisms 2015-04, Vol.20 (4), p.281-291 |
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| creator | Takeiwa, Toshihiko Taniguchi, Ichiro Ohno, Mutsuhito |
| description | The signal recognition particle is a ribonucleoprotein complex that is essential for the translocation of nascent proteins into the endoplasmic reticulum. It has been shown that the RNA component (SRP RNA) is exported from the nucleus by CRM1 in the budding yeast. However, how SRP RNA is exported in higher species has been elusive. Here, we show that SRP RNA does not use the CRM1 pathway in Xenopus oocytes. Instead, SRP RNA uses the same export pathway as pre‐miRNA and tRNA as showed by cross‐competition experiments. Consistently, the recombinant Exportin‐5 protein specifically stimulated export of SRP RNA as well as of pre‐miRNA and tRNA, whereas an antibody raised against Exportin‐5 specifically inhibited export of the same RNA species. Moreover, biotinylated SRP RNA can pull down Exportin‐5 but not CRM1 from HeLa cell nuclear extracts in a RanGTP‐dependent manner. These results, taken together, strongly suggest that the principal export receptor for SRP RNA in vertebrates is Exportin‐5 unlike in the budding yeast. |
| doi_str_mv | 10.1111/gtc.12218 |
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It has been shown that the RNA component (SRP RNA) is exported from the nucleus by CRM1 in the budding yeast. However, how SRP RNA is exported in higher species has been elusive. Here, we show that SRP RNA does not use the CRM1 pathway in Xenopus oocytes. Instead, SRP RNA uses the same export pathway as pre‐miRNA and tRNA as showed by cross‐competition experiments. Consistently, the recombinant Exportin‐5 protein specifically stimulated export of SRP RNA as well as of pre‐miRNA and tRNA, whereas an antibody raised against Exportin‐5 specifically inhibited export of the same RNA species. Moreover, biotinylated SRP RNA can pull down Exportin‐5 but not CRM1 from HeLa cell nuclear extracts in a RanGTP‐dependent manner. These results, taken together, strongly suggest that the principal export receptor for SRP RNA in vertebrates is Exportin‐5 unlike in the budding yeast.</description><identifier>ISSN: 1356-9597</identifier><identifier>EISSN: 1365-2443</identifier><identifier>DOI: 10.1111/gtc.12218</identifier><identifier>PMID: 25656399</identifier><identifier>CODEN: GECEFL</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Active Transport, Cell Nucleus ; Animals ; Cell Nucleus - metabolism ; Exportin 1 Protein ; Exports ; HeLa Cells ; Humans ; Karyopherins - metabolism ; Microinjections - methods ; MicroRNAs - metabolism ; Oocytes ; Original ; Receptors, Cytoplasmic and Nuclear - metabolism ; RNA - metabolism ; RNA, Transfer - metabolism ; Saccharomyces cerevisiae ; Signal Recognition Particle - metabolism ; Vertebrates ; Vertebrates - metabolism ; Xenopus</subject><ispartof>Genes to cells : devoted to molecular & cellular mechanisms, 2015-04, Vol.20 (4), p.281-291</ispartof><rights>2015 The Authors. published by Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.</rights><rights>2015 The Authors. Genes to Cells published by Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.</rights><rights>Copyright © 2015 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd</rights><rights>2015 The Authors. published by Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6128-90f5391882013d6ded6be01de7b85d5371969f3fb69a058f72c133edf1f8cc33</citedby><cites>FETCH-LOGICAL-c6128-90f5391882013d6ded6be01de7b85d5371969f3fb69a058f72c133edf1f8cc33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fgtc.12218$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fgtc.12218$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,315,781,785,886,1418,1434,27929,27930,45579,45580,46414,46838</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25656399$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takeiwa, Toshihiko</creatorcontrib><creatorcontrib>Taniguchi, Ichiro</creatorcontrib><creatorcontrib>Ohno, Mutsuhito</creatorcontrib><title>Exportin‐5 mediates nuclear export of SRP RNA in vertebrates</title><title>Genes to cells : devoted to molecular & cellular mechanisms</title><addtitle>Genes Cells</addtitle><description>The signal recognition particle is a ribonucleoprotein complex that is essential for the translocation of nascent proteins into the endoplasmic reticulum. It has been shown that the RNA component (SRP RNA) is exported from the nucleus by CRM1 in the budding yeast. However, how SRP RNA is exported in higher species has been elusive. Here, we show that SRP RNA does not use the CRM1 pathway in Xenopus oocytes. Instead, SRP RNA uses the same export pathway as pre‐miRNA and tRNA as showed by cross‐competition experiments. Consistently, the recombinant Exportin‐5 protein specifically stimulated export of SRP RNA as well as of pre‐miRNA and tRNA, whereas an antibody raised against Exportin‐5 specifically inhibited export of the same RNA species. Moreover, biotinylated SRP RNA can pull down Exportin‐5 but not CRM1 from HeLa cell nuclear extracts in a RanGTP‐dependent manner. These results, taken together, strongly suggest that the principal export receptor for SRP RNA in vertebrates is Exportin‐5 unlike in the budding yeast.</description><subject>Active Transport, Cell Nucleus</subject><subject>Animals</subject><subject>Cell Nucleus - metabolism</subject><subject>Exportin 1 Protein</subject><subject>Exports</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Karyopherins - metabolism</subject><subject>Microinjections - methods</subject><subject>MicroRNAs - metabolism</subject><subject>Oocytes</subject><subject>Original</subject><subject>Receptors, Cytoplasmic and Nuclear - metabolism</subject><subject>RNA - metabolism</subject><subject>RNA, Transfer - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Signal Recognition Particle - metabolism</subject><subject>Vertebrates</subject><subject>Vertebrates - metabolism</subject><subject>Xenopus</subject><issn>1356-9597</issn><issn>1365-2443</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNqNkc9KxDAQxoMo_lk9-AJS8KKHatI0aXIRZFlXQVTWvYc2nayVbrsmrbo3H8Fn9ElMd1VUEMxlAvPjm2_mQ2iX4CPi3_Gk0UckiohYQZuEchZGcUxXuz_joWQy2UBbzt1jTGiE2TraiBhnnEq5iU4Gz7PaNkX19vLKginkRdqAC6pWl5DaABbdoDbB7egmGF2dBkUVPIJtILMduI3WTFo62PmoPTQ-G4z75-Hl9fCif3oZak4iEUpsGJVEiMhbyHkOOc8AkxySTLCc0YRILg01GZcpZsIkkSaUQm6IEVpT2kMnS9lZm3mPGqrGpqWa2WKa2rmq00L97FTFnZrUjyqOiYj9zB46-BCw9UMLrlHTwmkoy7SCunWK8IT7w5H4PyiXwstK7NH9X-h93drKH6Kj_LIywZ35wyWlbe2cBfPlm2DV5ad8fmqRn2f3vi_6RX4G5oHjJfBUlDD_W0kNx_2l5DsohaO_</recordid><startdate>201504</startdate><enddate>201504</enddate><creator>Takeiwa, Toshihiko</creator><creator>Taniguchi, Ichiro</creator><creator>Ohno, Mutsuhito</creator><general>Wiley Subscription Services, Inc</general><general>Blackwell Publishing Ltd</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201504</creationdate><title>Exportin‐5 mediates nuclear export of SRP RNA in vertebrates</title><author>Takeiwa, Toshihiko ; Taniguchi, Ichiro ; Ohno, Mutsuhito</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6128-90f5391882013d6ded6be01de7b85d5371969f3fb69a058f72c133edf1f8cc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Active Transport, Cell Nucleus</topic><topic>Animals</topic><topic>Cell Nucleus - metabolism</topic><topic>Exportin 1 Protein</topic><topic>Exports</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Karyopherins - metabolism</topic><topic>Microinjections - methods</topic><topic>MicroRNAs - metabolism</topic><topic>Oocytes</topic><topic>Original</topic><topic>Receptors, Cytoplasmic and Nuclear - metabolism</topic><topic>RNA - metabolism</topic><topic>RNA, Transfer - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Signal Recognition Particle - metabolism</topic><topic>Vertebrates</topic><topic>Vertebrates - metabolism</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takeiwa, Toshihiko</creatorcontrib><creatorcontrib>Taniguchi, Ichiro</creatorcontrib><creatorcontrib>Ohno, Mutsuhito</creatorcontrib><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genes to cells : devoted to molecular & cellular mechanisms</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takeiwa, Toshihiko</au><au>Taniguchi, Ichiro</au><au>Ohno, Mutsuhito</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exportin‐5 mediates nuclear export of SRP RNA in vertebrates</atitle><jtitle>Genes to cells : devoted to molecular & cellular mechanisms</jtitle><addtitle>Genes Cells</addtitle><date>2015-04</date><risdate>2015</risdate><volume>20</volume><issue>4</issue><spage>281</spage><epage>291</epage><pages>281-291</pages><issn>1356-9597</issn><eissn>1365-2443</eissn><coden>GECEFL</coden><abstract>The signal recognition particle is a ribonucleoprotein complex that is essential for the translocation of nascent proteins into the endoplasmic reticulum. It has been shown that the RNA component (SRP RNA) is exported from the nucleus by CRM1 in the budding yeast. However, how SRP RNA is exported in higher species has been elusive. Here, we show that SRP RNA does not use the CRM1 pathway in Xenopus oocytes. Instead, SRP RNA uses the same export pathway as pre‐miRNA and tRNA as showed by cross‐competition experiments. Consistently, the recombinant Exportin‐5 protein specifically stimulated export of SRP RNA as well as of pre‐miRNA and tRNA, whereas an antibody raised against Exportin‐5 specifically inhibited export of the same RNA species. Moreover, biotinylated SRP RNA can pull down Exportin‐5 but not CRM1 from HeLa cell nuclear extracts in a RanGTP‐dependent manner. 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| subjects | Active Transport, Cell Nucleus Animals Cell Nucleus - metabolism Exportin 1 Protein Exports HeLa Cells Humans Karyopherins - metabolism Microinjections - methods MicroRNAs - metabolism Oocytes Original Receptors, Cytoplasmic and Nuclear - metabolism RNA - metabolism RNA, Transfer - metabolism Saccharomyces cerevisiae Signal Recognition Particle - metabolism Vertebrates Vertebrates - metabolism Xenopus |
| title | Exportin‐5 mediates nuclear export of SRP RNA in vertebrates |
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