Effects of er-miao-san extracts on TNF-alpha-induced MMP-1 expression in human dermal fibroblasts
Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity i...
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| Veröffentlicht in: | Biological research 2015-01, Vol.48 (1), p.8-8 |
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| creator | Bae, Seunghee Jung, Younjung Choi, Yeong Min Li, Shunhua |
| description | Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown.
This study investigated the effects of EMS on TNF-α-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-α-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IκB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-κB. Moreover, EMS reduced the level of nuclear p65 protein in HDFs. Luciferase assay revealed that EMS inhibits the transcriptional activity of NF-κB by stabilizing IκB. Our results show that EMS exerts its anti-inflammatory effect by inhibiting NF-κB-regulated genes such as IL-1β and IL-8. Moreover, EMS effectively inhibited TNF-α-induced expression of MMP-1 via the NF-κB pathway.
Taken together, our data suggest that EMS could potentially be used as an anti-inflammatory and anti-aging treatment. |
| doi_str_mv | 10.1186/0717-6287-48-8 |
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This study investigated the effects of EMS on TNF-α-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-α-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IκB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-κB. Moreover, EMS reduced the level of nuclear p65 protein in HDFs. Luciferase assay revealed that EMS inhibits the transcriptional activity of NF-κB by stabilizing IκB. Our results show that EMS exerts its anti-inflammatory effect by inhibiting NF-κB-regulated genes such as IL-1β and IL-8. Moreover, EMS effectively inhibited TNF-α-induced expression of MMP-1 via the NF-κB pathway.
Taken together, our data suggest that EMS could potentially be used as an anti-inflammatory and anti-aging treatment.</description><identifier>ISSN: 0717-6287</identifier><identifier>ISSN: 0716-9760</identifier><identifier>EISSN: 0717-6287</identifier><identifier>DOI: 10.1186/0717-6287-48-8</identifier><identifier>PMID: 25761492</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Aging - drug effects ; Analysis ; Anti-Inflammatory Agents - administration & dosage ; Care and treatment ; Cell Line ; Cell Survival - drug effects ; Dermis - cytology ; Drugs, Chinese Herbal - pharmacology ; Enzyme Induction - drug effects ; Fibroblasts ; Fibroblasts - drug effects ; Fibroblasts - enzymology ; Gene Expression Regulation - drug effects ; Humans ; Inflammation ; Interleukin-1beta - drug effects ; Interleukin-1beta - metabolism ; Interleukin-8 - drug effects ; Interleukin-8 - metabolism ; Matrix Metalloproteinase 1 - biosynthesis ; Mitogen-Activated Protein Kinases - drug effects ; NF-kappa B - metabolism ; Plant Extracts - pharmacology ; Real-Time Polymerase Chain Reaction ; Signal Transduction - drug effects ; Skin aging ; Tumor necrosis factor ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>Biological research, 2015-01, Vol.48 (1), p.8-8</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Bae et al.; licensee BioMed Central. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-ded42a2a95c2fbc2a97690e3559efbbf8e493308e0333700609570a5d21f78253</citedby><cites>FETCH-LOGICAL-c557t-ded42a2a95c2fbc2a97690e3559efbbf8e493308e0333700609570a5d21f78253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417304/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417304/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25761492$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bae, Seunghee</creatorcontrib><creatorcontrib>Jung, Younjung</creatorcontrib><creatorcontrib>Choi, Yeong Min</creatorcontrib><creatorcontrib>Li, Shunhua</creatorcontrib><title>Effects of er-miao-san extracts on TNF-alpha-induced MMP-1 expression in human dermal fibroblasts</title><title>Biological research</title><addtitle>Biol Res</addtitle><description>Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown.
This study investigated the effects of EMS on TNF-α-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-α-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IκB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-κB. Moreover, EMS reduced the level of nuclear p65 protein in HDFs. Luciferase assay revealed that EMS inhibits the transcriptional activity of NF-κB by stabilizing IκB. Our results show that EMS exerts its anti-inflammatory effect by inhibiting NF-κB-regulated genes such as IL-1β and IL-8. Moreover, EMS effectively inhibited TNF-α-induced expression of MMP-1 via the NF-κB pathway.
Taken together, our data suggest that EMS could potentially be used as an anti-inflammatory and anti-aging treatment.</description><subject>Aging - drug effects</subject><subject>Analysis</subject><subject>Anti-Inflammatory Agents - administration & dosage</subject><subject>Care and treatment</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Dermis - cytology</subject><subject>Drugs, Chinese Herbal - pharmacology</subject><subject>Enzyme Induction - drug effects</subject><subject>Fibroblasts</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - enzymology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Interleukin-1beta - drug effects</subject><subject>Interleukin-1beta - metabolism</subject><subject>Interleukin-8 - drug effects</subject><subject>Interleukin-8 - metabolism</subject><subject>Matrix Metalloproteinase 1 - biosynthesis</subject><subject>Mitogen-Activated Protein Kinases - drug effects</subject><subject>NF-kappa B - metabolism</subject><subject>Plant Extracts - pharmacology</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Signal Transduction - drug effects</subject><subject>Skin aging</subject><subject>Tumor necrosis factor</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>0717-6287</issn><issn>0716-9760</issn><issn>0717-6287</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkk1rFTEUhgdRbK1uXcqAG7tIzXcyG6GU1hZaFa3rkJk5uTcyk1yTmdL-ezO2vfRCySKHk-d9w_moqvcEHxGi5WesiEKSaoW4RvpFtb9NvHwS71Vvcv6DMRWYytfVHhVKEt7Q_cqeOgfdlOvoakho9DaibEMNt1Oy__Ohvv52huywWVvkQz930NdXVz8QKcwmQc6-ID7U63ksuh7SaIfa-TbFdrB5ym-rV84OGd493AfV77PT65NzdPn968XJ8SXqhFAT6qHn1FLbiI66tiuBkg0GJkQDrm2dBt4whjVgxpjCWOJGKGxFT4lTmgp2UH25993M7Qh9B6FUMJhN8qNNdyZab3Zfgl-bVbwxnBPFMC8Gnx4MUvw7Q57M6HMHw2ADxDkbIiWT5U_KCvrxHl3ZAYwPLi7dWnBzLDiRmmtFC3X0DFVOD6PvYgDnS35HcLgjKMxUBrGyc87m4tfPZ827FHNO4LaVEmyW1TDL9M0yfcO10UXw4Wl_tvjjLrB_rNGxrQ</recordid><startdate>20150126</startdate><enddate>20150126</enddate><creator>Bae, Seunghee</creator><creator>Jung, Younjung</creator><creator>Choi, Yeong Min</creator><creator>Li, Shunhua</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>INF</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150126</creationdate><title>Effects of er-miao-san extracts on TNF-alpha-induced MMP-1 expression in human dermal fibroblasts</title><author>Bae, Seunghee ; Jung, Younjung ; Choi, Yeong Min ; Li, Shunhua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-ded42a2a95c2fbc2a97690e3559efbbf8e493308e0333700609570a5d21f78253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aging - drug effects</topic><topic>Analysis</topic><topic>Anti-Inflammatory Agents - administration & dosage</topic><topic>Care and treatment</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Dermis - cytology</topic><topic>Drugs, Chinese Herbal - pharmacology</topic><topic>Enzyme Induction - drug effects</topic><topic>Fibroblasts</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - enzymology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Interleukin-1beta - drug effects</topic><topic>Interleukin-1beta - metabolism</topic><topic>Interleukin-8 - drug effects</topic><topic>Interleukin-8 - metabolism</topic><topic>Matrix Metalloproteinase 1 - biosynthesis</topic><topic>Mitogen-Activated Protein Kinases - drug effects</topic><topic>NF-kappa B - metabolism</topic><topic>Plant Extracts - pharmacology</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Signal Transduction - drug effects</topic><topic>Skin aging</topic><topic>Tumor necrosis factor</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bae, Seunghee</creatorcontrib><creatorcontrib>Jung, Younjung</creatorcontrib><creatorcontrib>Choi, Yeong Min</creatorcontrib><creatorcontrib>Li, Shunhua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>Gale OneFile: Informe Academico</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bae, Seunghee</au><au>Jung, Younjung</au><au>Choi, Yeong Min</au><au>Li, Shunhua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of er-miao-san extracts on TNF-alpha-induced MMP-1 expression in human dermal fibroblasts</atitle><jtitle>Biological research</jtitle><addtitle>Biol Res</addtitle><date>2015-01-26</date><risdate>2015</risdate><volume>48</volume><issue>1</issue><spage>8</spage><epage>8</epage><pages>8-8</pages><issn>0717-6287</issn><issn>0716-9760</issn><eissn>0717-6287</eissn><abstract>Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown.
This study investigated the effects of EMS on TNF-α-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-α-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IκB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-κB. Moreover, EMS reduced the level of nuclear p65 protein in HDFs. Luciferase assay revealed that EMS inhibits the transcriptional activity of NF-κB by stabilizing IκB. Our results show that EMS exerts its anti-inflammatory effect by inhibiting NF-κB-regulated genes such as IL-1β and IL-8. Moreover, EMS effectively inhibited TNF-α-induced expression of MMP-1 via the NF-κB pathway.
Taken together, our data suggest that EMS could potentially be used as an anti-inflammatory and anti-aging treatment.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>25761492</pmid><doi>10.1186/0717-6287-48-8</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aging - drug effects Analysis Anti-Inflammatory Agents - administration & dosage Care and treatment Cell Line Cell Survival - drug effects Dermis - cytology Drugs, Chinese Herbal - pharmacology Enzyme Induction - drug effects Fibroblasts Fibroblasts - drug effects Fibroblasts - enzymology Gene Expression Regulation - drug effects Humans Inflammation Interleukin-1beta - drug effects Interleukin-1beta - metabolism Interleukin-8 - drug effects Interleukin-8 - metabolism Matrix Metalloproteinase 1 - biosynthesis Mitogen-Activated Protein Kinases - drug effects NF-kappa B - metabolism Plant Extracts - pharmacology Real-Time Polymerase Chain Reaction Signal Transduction - drug effects Skin aging Tumor necrosis factor Tumor Necrosis Factor-alpha - metabolism |
| title | Effects of er-miao-san extracts on TNF-alpha-induced MMP-1 expression in human dermal fibroblasts |
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