Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation
Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed results. However, the cellular and molecular mechanisms of the neotissue development, valve thickening, and stenosis development are not researched ext...
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| Veröffentlicht in: | Journal of Visualized Experiments 2014-07 (89) |
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| creator | Lee, Yong-Ung Yi, Tai James, Iyore Tara, Shuhei Stuber, Alexander J. Shah, Kejal V. Lee, Avione Y. Sugiura, Tadahisa Hibino, Narutoshi Shinoka, Toshiharu Breuer, Christopher K. |
| description | Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed results. However, the cellular and molecular mechanisms of the neotissue development, valve thickening, and stenosis development are not researched extensively. To answer the above questions, we developed a murine heterotopic heart valve transplantation model. A heart valve was harvested from a valve donor mouse and transplanted to a heart donor mouse. The heart with a new valve was transplanted heterotopically to a recipient mouse. The transplanted heart showed its own heartbeat, independent of the recipient’s heartbeat. The blood flow was quantified using a high frequency ultrasound system with a pulsed wave Doppler. The flow through the implanted pulmonary valve showed forward flow with minimal regurgitation and the peak flow was close to 100 mm/sec. This murine model of heart valve transplantation is highly versatile, so it can be modified and adapted to provide different hemodynamic environments and/or can be used with various transgenic mice to study neotissue development in a tissue engineered heart valve. |
| doi_str_mv | 10.3791/51695 |
| format | Article |
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However, the cellular and molecular mechanisms of the neotissue development, valve thickening, and stenosis development are not researched extensively. To answer the above questions, we developed a murine heterotopic heart valve transplantation model. A heart valve was harvested from a valve donor mouse and transplanted to a heart donor mouse. The heart with a new valve was transplanted heterotopically to a recipient mouse. The transplanted heart showed its own heartbeat, independent of the recipient’s heartbeat. The blood flow was quantified using a high frequency ultrasound system with a pulsed wave Doppler. The flow through the implanted pulmonary valve showed forward flow with minimal regurgitation and the peak flow was close to 100 mm/sec. This murine model of heart valve transplantation is highly versatile, so it can be modified and adapted to provide different hemodynamic environments and/or can be used with various transgenic mice to study neotissue development in a tissue engineered heart valve.</description><identifier>ISSN: 1940-087X</identifier><identifier>EISSN: 1940-087X</identifier><identifier>DOI: 10.3791/51695</identifier><identifier>PMID: 25079013</identifier><language>eng</language><publisher>United States: MyJove Corporation</publisher><subject>Animals ; Blood Vessel Prosthesis ; Female ; Heart Transplantation - methods ; Medicine ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Models, Animal ; Pulmonary Valve - transplantation ; Tissue and Organ Harvesting - methods ; Transplantation, Heterotopic - methods</subject><ispartof>Journal of Visualized Experiments, 2014-07 (89)</ispartof><rights>Copyright © 2014, Journal of Visualized Experiments</rights><rights>Copyright © 2014, Journal of Visualized Experiments 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-11e59010b8535d52dd45f543111eafea5211c0ae1ba04043f1831d5c5ed666ca3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttps://www.jove.com/files/email_thumbs/51695.png</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4407638/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4407638/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3843,27924,27925,53791,53793</link.rule.ids><linktorsrc>$$Uhttp://dx.doi.org/10.3791/51695$$EView_record_in_Journal_of_Visualized_Experiments$$FView_record_in_$$GJournal_of_Visualized_Experiments</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25079013$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Yong-Ung</creatorcontrib><creatorcontrib>Yi, Tai</creatorcontrib><creatorcontrib>James, Iyore</creatorcontrib><creatorcontrib>Tara, Shuhei</creatorcontrib><creatorcontrib>Stuber, Alexander J.</creatorcontrib><creatorcontrib>Shah, Kejal V.</creatorcontrib><creatorcontrib>Lee, Avione Y.</creatorcontrib><creatorcontrib>Sugiura, Tadahisa</creatorcontrib><creatorcontrib>Hibino, Narutoshi</creatorcontrib><creatorcontrib>Shinoka, Toshiharu</creatorcontrib><creatorcontrib>Breuer, Christopher K.</creatorcontrib><title>Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation</title><title>Journal of Visualized Experiments</title><addtitle>J Vis Exp</addtitle><description>Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed results. However, the cellular and molecular mechanisms of the neotissue development, valve thickening, and stenosis development are not researched extensively. To answer the above questions, we developed a murine heterotopic heart valve transplantation model. A heart valve was harvested from a valve donor mouse and transplanted to a heart donor mouse. The heart with a new valve was transplanted heterotopically to a recipient mouse. The transplanted heart showed its own heartbeat, independent of the recipient’s heartbeat. The blood flow was quantified using a high frequency ultrasound system with a pulsed wave Doppler. The flow through the implanted pulmonary valve showed forward flow with minimal regurgitation and the peak flow was close to 100 mm/sec. This murine model of heart valve transplantation is highly versatile, so it can be modified and adapted to provide different hemodynamic environments and/or can be used with various transgenic mice to study neotissue development in a tissue engineered heart valve.</description><subject>Animals</subject><subject>Blood Vessel Prosthesis</subject><subject>Female</subject><subject>Heart Transplantation - methods</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Models, Animal</subject><subject>Pulmonary Valve - transplantation</subject><subject>Tissue and Organ Harvesting - methods</subject><subject>Transplantation, Heterotopic - methods</subject><issn>1940-087X</issn><issn>1940-087X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1Lw0AQhhdRbK39Ax4kF8FLdCebzcdFkKJWqOihFcHDsk0mNSXJxt1NwX_v1tZSvcwOzDPvzjtDyBDoFYtTuOYQpfyA9CENqU-T-O1wL--RE2OWlEYB5ckx6QWcxikF1ifvUy0b01aysdKWqvFU4b10Va0aqb-8V1mt0JuZsll40ntSnUEXc6zW2BgtamVVW2Yul9p6_7ROyVEhK4PD7Tsgs_u76WjsT54fHke3Ez8LeWB9AORuGDpPOOM5D_I85AUPGbiCLFDyACCjEmEuaUhDVkDCIOcZxzyKokyyAbnZ6LbdvMY8w8ZqWYlWl7UzIZQsxd9KU36IhVqJMKRxxBIncLkV0OqzQ2NFXZoMK-cEnWcBnAOFJI6ZQy82aKaVMRqL3TdAxfoQ4ucQjjvfn2lH_W7eAWcbYKlWKJaq043b0bb7G5WWjMY</recordid><startdate>20140723</startdate><enddate>20140723</enddate><creator>Lee, Yong-Ung</creator><creator>Yi, Tai</creator><creator>James, Iyore</creator><creator>Tara, Shuhei</creator><creator>Stuber, Alexander J.</creator><creator>Shah, Kejal V.</creator><creator>Lee, Avione Y.</creator><creator>Sugiura, Tadahisa</creator><creator>Hibino, Narutoshi</creator><creator>Shinoka, Toshiharu</creator><creator>Breuer, Christopher K.</creator><general>MyJove Corporation</general><scope>BTACS</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140723</creationdate><title>Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation</title><author>Lee, Yong-Ung ; Yi, Tai ; James, Iyore ; Tara, Shuhei ; Stuber, Alexander J. ; Shah, Kejal V. ; Lee, Avione Y. ; Sugiura, Tadahisa ; Hibino, Narutoshi ; Shinoka, Toshiharu ; Breuer, Christopher K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-11e59010b8535d52dd45f543111eafea5211c0ae1ba04043f1831d5c5ed666ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Blood Vessel Prosthesis</topic><topic>Female</topic><topic>Heart Transplantation - methods</topic><topic>Medicine</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Models, Animal</topic><topic>Pulmonary Valve - transplantation</topic><topic>Tissue and Organ Harvesting - methods</topic><topic>Transplantation, Heterotopic - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Yong-Ung</creatorcontrib><creatorcontrib>Yi, Tai</creatorcontrib><creatorcontrib>James, Iyore</creatorcontrib><creatorcontrib>Tara, Shuhei</creatorcontrib><creatorcontrib>Stuber, Alexander J.</creatorcontrib><creatorcontrib>Shah, Kejal V.</creatorcontrib><creatorcontrib>Lee, Avione Y.</creatorcontrib><creatorcontrib>Sugiura, Tadahisa</creatorcontrib><creatorcontrib>Hibino, Narutoshi</creatorcontrib><creatorcontrib>Shinoka, Toshiharu</creatorcontrib><creatorcontrib>Breuer, Christopher K.</creatorcontrib><collection>JoVE Journal: Medicine</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Visualized Experiments</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Lee, Yong-Ung</au><au>Yi, Tai</au><au>James, Iyore</au><au>Tara, Shuhei</au><au>Stuber, Alexander J.</au><au>Shah, Kejal V.</au><au>Lee, Avione Y.</au><au>Sugiura, Tadahisa</au><au>Hibino, Narutoshi</au><au>Shinoka, Toshiharu</au><au>Breuer, Christopher K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation</atitle><jtitle>Journal of Visualized Experiments</jtitle><addtitle>J Vis Exp</addtitle><date>2014-07-23</date><risdate>2014</risdate><issue>89</issue><issn>1940-087X</issn><eissn>1940-087X</eissn><abstract>Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed results. However, the cellular and molecular mechanisms of the neotissue development, valve thickening, and stenosis development are not researched extensively. To answer the above questions, we developed a murine heterotopic heart valve transplantation model. A heart valve was harvested from a valve donor mouse and transplanted to a heart donor mouse. The heart with a new valve was transplanted heterotopically to a recipient mouse. The transplanted heart showed its own heartbeat, independent of the recipient’s heartbeat. The blood flow was quantified using a high frequency ultrasound system with a pulsed wave Doppler. The flow through the implanted pulmonary valve showed forward flow with minimal regurgitation and the peak flow was close to 100 mm/sec. This murine model of heart valve transplantation is highly versatile, so it can be modified and adapted to provide different hemodynamic environments and/or can be used with various transgenic mice to study neotissue development in a tissue engineered heart valve.</abstract><cop>United States</cop><pub>MyJove Corporation</pub><pmid>25079013</pmid><doi>10.3791/51695</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Blood Vessel Prosthesis Female Heart Transplantation - methods Medicine Mice Mice, Inbred C57BL Mice, Transgenic Models, Animal Pulmonary Valve - transplantation Tissue and Organ Harvesting - methods Transplantation, Heterotopic - methods |
| title | Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation |
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