Digital microfluidic three-dimensional cell culture and chemical screening platform using alginate hydrogels
Electro wetting-on-dielectric (EWOD) digital microfluidics (DMF) can be used to develop improved chemical screening platforms using 3-dimensional (3D) cell culture. Alginate hydrogels are one common method by which a 3D cell culture environment is created. This paper presents a study of alginate gel...
Gespeichert in:
Veröffentlicht in: | Biomicrofluidics 2015-03, Vol.9 (2), p.024116-024116 |
---|---|
Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 024116 |
---|---|
container_issue | 2 |
container_start_page | 024116 |
container_title | Biomicrofluidics |
container_volume | 9 |
creator | George, Subin M Moon, Hyejin |
description | Electro wetting-on-dielectric (EWOD) digital microfluidics (DMF) can be used to develop improved chemical screening platforms using 3-dimensional (3D) cell culture. Alginate hydrogels are one common method by which a 3D cell culture environment is created. This paper presents a study of alginate gelation on EWOD DMF and investigates designs to obtain uniform alginate hydrogels that can be repeatedly addressed by any desired liquids. A design which allows for gels to be retained in place during liquid delivery and removal without using any physical barriers or hydrophilic patterning of substrates is presented. A proof of concept screening platform is demonstrated by examining the effects of different concentrations of a test chemical on 3D cells in alginate hydrogels. In addition, the temporal effects of the various chemical concentrations on different hydrogel posts are demonstrated, thereby establishing the benefits of an EWOD DMF 3D cell culture and chemical screening platform using alginate hydrogels. |
doi_str_mv | 10.1063/1.4918377 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4401805</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2124809782</sourcerecordid><originalsourceid>FETCH-LOGICAL-c403t-ce3b728fcf679b42e8671c110ac6341f75da108fa27471b4f91d563e30bfd8d73</originalsourceid><addsrcrecordid>eNpdkU1rHSEYhaUkNGnaRf9AGcimXUzqq87obAIlbT4gkE27FsePuQZHb3SmkH9fL7kNSTZ-cB6PL-cg9BnwGeCefoczNoCgnL9DxzBQ0gLuxMGL8xH6UMo9xh1wQt6jI9INrANGjlH46Se_qNDMXufkwuqN182yyda2xs82Fp9ilbUNdVnDsmbbqGgavbH1SVWKrmz0cWq2QS0u5blZy-6qwuSjWmyzeTQ5TTaUj-jQqVDsp_1-gv5c_vp9cd3e3l3dXPy4bTXDdGm1pSMnwmnX82FkxIqegwbASveUgeOdUYCFU4QzDiNzA5iup5bi0RlhOD1B50--23WcrdE2LlkFuc1-VvlRJuXlayX6jZzSX8kYBoG7avB1b5DTw2rLImdfdhGoaNNaJPSigkD73V-nb9D7tOYaWZEECBN44IJU6tsTVUMuJVv3PAxguetQgtx3WNkvL6d_Jv-XRv8BAmmYqg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2124809782</pqid></control><display><type>article</type><title>Digital microfluidic three-dimensional cell culture and chemical screening platform using alginate hydrogels</title><source>AIP Journals Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>George, Subin M ; Moon, Hyejin</creator><creatorcontrib>George, Subin M ; Moon, Hyejin</creatorcontrib><description>Electro wetting-on-dielectric (EWOD) digital microfluidics (DMF) can be used to develop improved chemical screening platforms using 3-dimensional (3D) cell culture. Alginate hydrogels are one common method by which a 3D cell culture environment is created. This paper presents a study of alginate gelation on EWOD DMF and investigates designs to obtain uniform alginate hydrogels that can be repeatedly addressed by any desired liquids. A design which allows for gels to be retained in place during liquid delivery and removal without using any physical barriers or hydrophilic patterning of substrates is presented. A proof of concept screening platform is demonstrated by examining the effects of different concentrations of a test chemical on 3D cells in alginate hydrogels. In addition, the temporal effects of the various chemical concentrations on different hydrogel posts are demonstrated, thereby establishing the benefits of an EWOD DMF 3D cell culture and chemical screening platform using alginate hydrogels.</description><identifier>ISSN: 1932-1058</identifier><identifier>EISSN: 1932-1058</identifier><identifier>DOI: 10.1063/1.4918377</identifier><identifier>PMID: 25945142</identifier><language>eng</language><publisher>United States: American Institute of Physics</publisher><subject>Alginates ; Cell culture ; Gelation ; Gels ; Hydrogels ; Microfluidics ; Organic chemistry ; Regular ; Screening ; Substrates</subject><ispartof>Biomicrofluidics, 2015-03, Vol.9 (2), p.024116-024116</ispartof><rights>2015 AIP Publishing LLC.</rights><rights>Copyright © 2015 AIP Publishing LLC 2015 AIP Publishing LLC</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-ce3b728fcf679b42e8671c110ac6341f75da108fa27471b4f91d563e30bfd8d73</citedby><cites>FETCH-LOGICAL-c403t-ce3b728fcf679b42e8671c110ac6341f75da108fa27471b4f91d563e30bfd8d73</cites><orcidid>0000-0002-8904-2009 ; 0000-0003-2965-1520</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401805/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401805/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25945142$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>George, Subin M</creatorcontrib><creatorcontrib>Moon, Hyejin</creatorcontrib><title>Digital microfluidic three-dimensional cell culture and chemical screening platform using alginate hydrogels</title><title>Biomicrofluidics</title><addtitle>Biomicrofluidics</addtitle><description>Electro wetting-on-dielectric (EWOD) digital microfluidics (DMF) can be used to develop improved chemical screening platforms using 3-dimensional (3D) cell culture. Alginate hydrogels are one common method by which a 3D cell culture environment is created. This paper presents a study of alginate gelation on EWOD DMF and investigates designs to obtain uniform alginate hydrogels that can be repeatedly addressed by any desired liquids. A design which allows for gels to be retained in place during liquid delivery and removal without using any physical barriers or hydrophilic patterning of substrates is presented. A proof of concept screening platform is demonstrated by examining the effects of different concentrations of a test chemical on 3D cells in alginate hydrogels. In addition, the temporal effects of the various chemical concentrations on different hydrogel posts are demonstrated, thereby establishing the benefits of an EWOD DMF 3D cell culture and chemical screening platform using alginate hydrogels.</description><subject>Alginates</subject><subject>Cell culture</subject><subject>Gelation</subject><subject>Gels</subject><subject>Hydrogels</subject><subject>Microfluidics</subject><subject>Organic chemistry</subject><subject>Regular</subject><subject>Screening</subject><subject>Substrates</subject><issn>1932-1058</issn><issn>1932-1058</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNpdkU1rHSEYhaUkNGnaRf9AGcimXUzqq87obAIlbT4gkE27FsePuQZHb3SmkH9fL7kNSTZ-cB6PL-cg9BnwGeCefoczNoCgnL9DxzBQ0gLuxMGL8xH6UMo9xh1wQt6jI9INrANGjlH46Se_qNDMXufkwuqN182yyda2xs82Fp9ilbUNdVnDsmbbqGgavbH1SVWKrmz0cWq2QS0u5blZy-6qwuSjWmyzeTQ5TTaUj-jQqVDsp_1-gv5c_vp9cd3e3l3dXPy4bTXDdGm1pSMnwmnX82FkxIqegwbASveUgeOdUYCFU4QzDiNzA5iup5bi0RlhOD1B50--23WcrdE2LlkFuc1-VvlRJuXlayX6jZzSX8kYBoG7avB1b5DTw2rLImdfdhGoaNNaJPSigkD73V-nb9D7tOYaWZEECBN44IJU6tsTVUMuJVv3PAxguetQgtx3WNkvL6d_Jv-XRv8BAmmYqg</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>George, Subin M</creator><creator>Moon, Hyejin</creator><general>American Institute of Physics</general><general>AIP Publishing LLC</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8904-2009</orcidid><orcidid>https://orcid.org/0000-0003-2965-1520</orcidid></search><sort><creationdate>20150301</creationdate><title>Digital microfluidic three-dimensional cell culture and chemical screening platform using alginate hydrogels</title><author>George, Subin M ; Moon, Hyejin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-ce3b728fcf679b42e8671c110ac6341f75da108fa27471b4f91d563e30bfd8d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Alginates</topic><topic>Cell culture</topic><topic>Gelation</topic><topic>Gels</topic><topic>Hydrogels</topic><topic>Microfluidics</topic><topic>Organic chemistry</topic><topic>Regular</topic><topic>Screening</topic><topic>Substrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>George, Subin M</creatorcontrib><creatorcontrib>Moon, Hyejin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomicrofluidics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>George, Subin M</au><au>Moon, Hyejin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Digital microfluidic three-dimensional cell culture and chemical screening platform using alginate hydrogels</atitle><jtitle>Biomicrofluidics</jtitle><addtitle>Biomicrofluidics</addtitle><date>2015-03-01</date><risdate>2015</risdate><volume>9</volume><issue>2</issue><spage>024116</spage><epage>024116</epage><pages>024116-024116</pages><issn>1932-1058</issn><eissn>1932-1058</eissn><abstract>Electro wetting-on-dielectric (EWOD) digital microfluidics (DMF) can be used to develop improved chemical screening platforms using 3-dimensional (3D) cell culture. Alginate hydrogels are one common method by which a 3D cell culture environment is created. This paper presents a study of alginate gelation on EWOD DMF and investigates designs to obtain uniform alginate hydrogels that can be repeatedly addressed by any desired liquids. A design which allows for gels to be retained in place during liquid delivery and removal without using any physical barriers or hydrophilic patterning of substrates is presented. A proof of concept screening platform is demonstrated by examining the effects of different concentrations of a test chemical on 3D cells in alginate hydrogels. In addition, the temporal effects of the various chemical concentrations on different hydrogel posts are demonstrated, thereby establishing the benefits of an EWOD DMF 3D cell culture and chemical screening platform using alginate hydrogels.</abstract><cop>United States</cop><pub>American Institute of Physics</pub><pmid>25945142</pmid><doi>10.1063/1.4918377</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-8904-2009</orcidid><orcidid>https://orcid.org/0000-0003-2965-1520</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-1058 |
ispartof | Biomicrofluidics, 2015-03, Vol.9 (2), p.024116-024116 |
issn | 1932-1058 1932-1058 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4401805 |
source | AIP Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
subjects | Alginates Cell culture Gelation Gels Hydrogels Microfluidics Organic chemistry Regular Screening Substrates |
title | Digital microfluidic three-dimensional cell culture and chemical screening platform using alginate hydrogels |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T04%3A52%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Digital%20microfluidic%20three-dimensional%20cell%20culture%20and%20chemical%20screening%20platform%20using%20alginate%20hydrogels&rft.jtitle=Biomicrofluidics&rft.au=George,%20Subin%20M&rft.date=2015-03-01&rft.volume=9&rft.issue=2&rft.spage=024116&rft.epage=024116&rft.pages=024116-024116&rft.issn=1932-1058&rft.eissn=1932-1058&rft_id=info:doi/10.1063/1.4918377&rft_dat=%3Cproquest_pubme%3E2124809782%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2124809782&rft_id=info:pmid/25945142&rfr_iscdi=true |