Contribution of neonatal amniotic fluid testing to diagnosis of congenital toxoplasmosis
We evaluated the molecular diagnosis of congenital toxoplasmosis (CT) on neonatal amniotic fluid samples from 488 mother-child pairs. Maternal infection during pregnancy was diagnosed and dated or could not be ruled out. Forty-six cases of CT were defined according to the European Research Network o...
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| Veröffentlicht in: | Journal of clinical microbiology 2015-05, Vol.53 (5), p.1719-1721 |
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| container_title | Journal of clinical microbiology |
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| creator | Filisetti, Denis Yera, Hélène Villard, Odile Escande, Benoît Wafo, Estelle Houfflin-Debarge, Véronique Delhaes, Laurence Bastien, Patrick |
| description | We evaluated the molecular diagnosis of congenital toxoplasmosis (CT) on neonatal amniotic fluid samples from 488 mother-child pairs. Maternal infection during pregnancy was diagnosed and dated or could not be ruled out. Forty-six cases of CT were defined according to the European Research Network on CT classification system and case definitions. Neonatal amniotic fluid testing had an overall sensitivity of 54% (95% confidence interval [95% CI], 39 to 69%) and a specificity of 100% (95% CI, 99 to 100%). Its sensitivity was 33% (95% CI, 13 to 59%) when antenatal diagnosis was positive and 68% (95% CI, 48 to 84%) when antenatal diagnosis was negative or lacking. This difference in sensitivity may have been due to treatment of antenatally diagnosed cases. Relative to postnatal serology, neonatal amniotic fluid testing allowed an earlier diagnosis to be made in 26% of the cases (95% CI, 9 to 51%). |
| doi_str_mv | 10.1128/JCM.02358-14 |
| format | Article |
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H.</contributor><creatorcontrib>Filisetti, Denis ; Yera, Hélène ; Villard, Odile ; Escande, Benoît ; Wafo, Estelle ; Houfflin-Debarge, Véronique ; Delhaes, Laurence ; Bastien, Patrick ; Gilligan, P. H.</creatorcontrib><description>We evaluated the molecular diagnosis of congenital toxoplasmosis (CT) on neonatal amniotic fluid samples from 488 mother-child pairs. Maternal infection during pregnancy was diagnosed and dated or could not be ruled out. Forty-six cases of CT were defined according to the European Research Network on CT classification system and case definitions. Neonatal amniotic fluid testing had an overall sensitivity of 54% (95% confidence interval [95% CI], 39 to 69%) and a specificity of 100% (95% CI, 99 to 100%). Its sensitivity was 33% (95% CI, 13 to 59%) when antenatal diagnosis was positive and 68% (95% CI, 48 to 84%) when antenatal diagnosis was negative or lacking. This difference in sensitivity may have been due to treatment of antenatally diagnosed cases. Relative to postnatal serology, neonatal amniotic fluid testing allowed an earlier diagnosis to be made in 26% of the cases (95% CI, 9 to 51%).</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.02358-14</identifier><identifier>PMID: 25694528</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Amniotic Fluid - parasitology ; Female ; Humans ; Infant, Newborn ; Life Sciences ; Microbiology and Parasitology ; Molecular Diagnostic Techniques - methods ; Parasitology ; Polymerase Chain Reaction - methods ; Pregnancy ; Sensitivity and Specificity ; Time Factors ; Toxoplasmosis, Congenital - diagnosis ; Toxoplasmosis, Congenital - parasitology</subject><ispartof>Journal of clinical microbiology, 2015-05, Vol.53 (5), p.1719-1721</ispartof><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved. 2015 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-950b7a2604efba744be398f8d80846d7cb0a219603d3eb0632d44d0fc8ecfea23</citedby><cites>FETCH-LOGICAL-c451t-950b7a2604efba744be398f8d80846d7cb0a219603d3eb0632d44d0fc8ecfea23</cites><orcidid>0000-0002-0525-0012</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400781/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400781/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3186,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25694528$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.umontpellier.fr/hal-02121059$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Gilligan, P. H.</contributor><creatorcontrib>Filisetti, Denis</creatorcontrib><creatorcontrib>Yera, Hélène</creatorcontrib><creatorcontrib>Villard, Odile</creatorcontrib><creatorcontrib>Escande, Benoît</creatorcontrib><creatorcontrib>Wafo, Estelle</creatorcontrib><creatorcontrib>Houfflin-Debarge, Véronique</creatorcontrib><creatorcontrib>Delhaes, Laurence</creatorcontrib><creatorcontrib>Bastien, Patrick</creatorcontrib><title>Contribution of neonatal amniotic fluid testing to diagnosis of congenital toxoplasmosis</title><title>Journal of clinical microbiology</title><addtitle>J Clin Microbiol</addtitle><description>We evaluated the molecular diagnosis of congenital toxoplasmosis (CT) on neonatal amniotic fluid samples from 488 mother-child pairs. Maternal infection during pregnancy was diagnosed and dated or could not be ruled out. Forty-six cases of CT were defined according to the European Research Network on CT classification system and case definitions. Neonatal amniotic fluid testing had an overall sensitivity of 54% (95% confidence interval [95% CI], 39 to 69%) and a specificity of 100% (95% CI, 99 to 100%). Its sensitivity was 33% (95% CI, 13 to 59%) when antenatal diagnosis was positive and 68% (95% CI, 48 to 84%) when antenatal diagnosis was negative or lacking. This difference in sensitivity may have been due to treatment of antenatally diagnosed cases. Relative to postnatal serology, neonatal amniotic fluid testing allowed an earlier diagnosis to be made in 26% of the cases (95% CI, 9 to 51%).</description><subject>Amniotic Fluid - parasitology</subject><subject>Female</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Life Sciences</subject><subject>Microbiology and Parasitology</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Parasitology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Pregnancy</subject><subject>Sensitivity and Specificity</subject><subject>Time Factors</subject><subject>Toxoplasmosis, Congenital - diagnosis</subject><subject>Toxoplasmosis, Congenital - parasitology</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1P3DAQhq0KVBbaW89VjlRqYMaxHeeChFYtH1rUSytxsxzHWVwl9jZ2EPx7EpYi4MRppJln3vl4CfmCcIRI5fHl8uoIaMFljuwDWSBUMhcCrnfIAqDiOWJR7pH9GP8CIGOcfyR7lIuKcSoX5HoZfBpcPSYXfBbazNvgddJdpnvvQnIma7vRNVmyMTm_zlLIGqfXPkQXZ94Ev7bezR0p3IVNp2M_1z6R3VZ30X5-igfkz88fv5fn-erX2cXydJUbxjHlFYe61FQAs22tS8ZqW1SylY0EyURTmho0xUpA0RS2BlHQhrEGWiOtaa2mxQE52epuxrq3jbHTObpTm8H1erhXQTv1uuLdjVqHW8UYQClxEvi2Fbh503Z-ulJzDihSBF7dzuzh07Ah_Bunj6jeRWO7Tk9vG6NCIUshSsnpO9CSUUAOMKHft6gZQoyDbZ_XQFCzx2ryWD16rJBN-NeXFz_D_00tHgCa3qM_</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Filisetti, Denis</creator><creator>Yera, Hélène</creator><creator>Villard, Odile</creator><creator>Escande, Benoît</creator><creator>Wafo, Estelle</creator><creator>Houfflin-Debarge, Véronique</creator><creator>Delhaes, Laurence</creator><creator>Bastien, Patrick</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>M7N</scope><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0525-0012</orcidid></search><sort><creationdate>20150501</creationdate><title>Contribution of neonatal amniotic fluid testing to diagnosis of congenital toxoplasmosis</title><author>Filisetti, Denis ; Yera, Hélène ; Villard, Odile ; Escande, Benoît ; Wafo, Estelle ; Houfflin-Debarge, Véronique ; Delhaes, Laurence ; Bastien, Patrick</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-950b7a2604efba744be398f8d80846d7cb0a219603d3eb0632d44d0fc8ecfea23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amniotic Fluid - parasitology</topic><topic>Female</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Life Sciences</topic><topic>Microbiology and Parasitology</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Parasitology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Pregnancy</topic><topic>Sensitivity and Specificity</topic><topic>Time Factors</topic><topic>Toxoplasmosis, Congenital - diagnosis</topic><topic>Toxoplasmosis, Congenital - parasitology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Filisetti, Denis</creatorcontrib><creatorcontrib>Yera, Hélène</creatorcontrib><creatorcontrib>Villard, Odile</creatorcontrib><creatorcontrib>Escande, Benoît</creatorcontrib><creatorcontrib>Wafo, Estelle</creatorcontrib><creatorcontrib>Houfflin-Debarge, Véronique</creatorcontrib><creatorcontrib>Delhaes, Laurence</creatorcontrib><creatorcontrib>Bastien, Patrick</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Filisetti, Denis</au><au>Yera, Hélène</au><au>Villard, Odile</au><au>Escande, Benoît</au><au>Wafo, Estelle</au><au>Houfflin-Debarge, Véronique</au><au>Delhaes, Laurence</au><au>Bastien, Patrick</au><au>Gilligan, P. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Contribution of neonatal amniotic fluid testing to diagnosis of congenital toxoplasmosis</atitle><jtitle>Journal of clinical microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>53</volume><issue>5</issue><spage>1719</spage><epage>1721</epage><pages>1719-1721</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><abstract>We evaluated the molecular diagnosis of congenital toxoplasmosis (CT) on neonatal amniotic fluid samples from 488 mother-child pairs. Maternal infection during pregnancy was diagnosed and dated or could not be ruled out. Forty-six cases of CT were defined according to the European Research Network on CT classification system and case definitions. Neonatal amniotic fluid testing had an overall sensitivity of 54% (95% confidence interval [95% CI], 39 to 69%) and a specificity of 100% (95% CI, 99 to 100%). Its sensitivity was 33% (95% CI, 13 to 59%) when antenatal diagnosis was positive and 68% (95% CI, 48 to 84%) when antenatal diagnosis was negative or lacking. This difference in sensitivity may have been due to treatment of antenatally diagnosed cases. Relative to postnatal serology, neonatal amniotic fluid testing allowed an earlier diagnosis to be made in 26% of the cases (95% CI, 9 to 51%).</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>25694528</pmid><doi>10.1128/JCM.02358-14</doi><tpages>3</tpages><orcidid>https://orcid.org/0000-0002-0525-0012</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Amniotic Fluid - parasitology Female Humans Infant, Newborn Life Sciences Microbiology and Parasitology Molecular Diagnostic Techniques - methods Parasitology Polymerase Chain Reaction - methods Pregnancy Sensitivity and Specificity Time Factors Toxoplasmosis, Congenital - diagnosis Toxoplasmosis, Congenital - parasitology |
| title | Contribution of neonatal amniotic fluid testing to diagnosis of congenital toxoplasmosis |
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