Mitochondrial DNA alteration in obstructive sleep apnea
Obstructive Sleep Apnea (OSAS) is a disease associated with the increase of cardiovascular risk and it is characterized by repeated episodes of Intermittent Hypoxia (IH) which inducing oxidative stress and systemic inflammation. Mitochondria are cell organelles involved in the respiratory that have...
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| Veröffentlicht in: | Respiratory research 2015-04, Vol.16 (1), p.47-47, Article 47 |
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| creator | Lacedonia, Donato Carpagnano, Giovanna E Crisetti, Elisabetta Cotugno, Grazia Palladino, Grazia P Patricelli, Giulia Sabato, Roberto Foschino Barbaro, Maria P |
| description | Obstructive Sleep Apnea (OSAS) is a disease associated with the increase of cardiovascular risk and it is characterized by repeated episodes of Intermittent Hypoxia (IH) which inducing oxidative stress and systemic inflammation. Mitochondria are cell organelles involved in the respiratory that have their own DNA (MtDNA). The aim of this study was to investigate if the increase of oxidative stress in OSAS patients can induce also MtDNA alterations.
46 OSAS patients (age 59.27 ± 11.38; BMI 30.84 ± 3.64; AHI 36.63 ± 24.18) were compared with 36 control subjects (age 54.42 ± 6.63; BMI 29.06 ± 4.7; AHI 3.8 ± 1.10). In blood cells Content of MtDNA and nuclear DNA (nDNA) was measured in OSAS patients by Real Time PCR. The ratio between MtDNA/nDNA was then calculated. Presence of oxidative stress was evaluated by levels of Reactive Oxygen Metabolites (ROMs), measured by diacron reactive oxygen metabolite test (d-ROM test).
MtDNA/nDNA was higher in patients with OSAS than in the control group (150.94 ± 49.14 vs 128.96 ± 45.8; p = 0.04), the levels of ROMs were also higher in OSAS subjects (329.71 ± 70.17 vs 226 ± 36.76; p = 0.04) and they were positively correlated with MtDNA/nDNA (R = 0.5, p < 0.01).
In OSAS patients there is a Mitochondrial DNA damage induced by the increase of oxidative stress. Intermittent hypoxia seems to be the main mechanism which leads to this process. |
| doi_str_mv | 10.1186/s12931-015-0205-7 |
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46 OSAS patients (age 59.27 ± 11.38; BMI 30.84 ± 3.64; AHI 36.63 ± 24.18) were compared with 36 control subjects (age 54.42 ± 6.63; BMI 29.06 ± 4.7; AHI 3.8 ± 1.10). In blood cells Content of MtDNA and nuclear DNA (nDNA) was measured in OSAS patients by Real Time PCR. The ratio between MtDNA/nDNA was then calculated. Presence of oxidative stress was evaluated by levels of Reactive Oxygen Metabolites (ROMs), measured by diacron reactive oxygen metabolite test (d-ROM test).
MtDNA/nDNA was higher in patients with OSAS than in the control group (150.94 ± 49.14 vs 128.96 ± 45.8; p = 0.04), the levels of ROMs were also higher in OSAS subjects (329.71 ± 70.17 vs 226 ± 36.76; p = 0.04) and they were positively correlated with MtDNA/nDNA (R = 0.5, p < 0.01).
In OSAS patients there is a Mitochondrial DNA damage induced by the increase of oxidative stress. Intermittent hypoxia seems to be the main mechanism which leads to this process.</description><identifier>ISSN: 1465-993X</identifier><identifier>ISSN: 1465-9921</identifier><identifier>EISSN: 1465-993X</identifier><identifier>DOI: 10.1186/s12931-015-0205-7</identifier><identifier>PMID: 25890226</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Aged ; Cardiovascular diseases ; Case-Control Studies ; DNA Damage ; DNA, Mitochondrial - blood ; DNA, Mitochondrial - genetics ; Female ; Genetic aspects ; Health aspects ; Humans ; Male ; Middle Aged ; Oxidative Stress ; Reactive Oxygen Species - blood ; Real-Time Polymerase Chain Reaction ; Risk factors ; Sleep Apnea, Obstructive - blood ; Sleep Apnea, Obstructive - genetics</subject><ispartof>Respiratory research, 2015-04, Vol.16 (1), p.47-47, Article 47</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Lacedonia et al.; licensee BioMed Central. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-792286d05bfbdaebe855db83af703436c07ccce962cc662a81956e7fed05f3583</citedby><cites>FETCH-LOGICAL-c466t-792286d05bfbdaebe855db83af703436c07ccce962cc662a81956e7fed05f3583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392628/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392628/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25890226$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lacedonia, Donato</creatorcontrib><creatorcontrib>Carpagnano, Giovanna E</creatorcontrib><creatorcontrib>Crisetti, Elisabetta</creatorcontrib><creatorcontrib>Cotugno, Grazia</creatorcontrib><creatorcontrib>Palladino, Grazia P</creatorcontrib><creatorcontrib>Patricelli, Giulia</creatorcontrib><creatorcontrib>Sabato, Roberto</creatorcontrib><creatorcontrib>Foschino Barbaro, Maria P</creatorcontrib><title>Mitochondrial DNA alteration in obstructive sleep apnea</title><title>Respiratory research</title><addtitle>Respir Res</addtitle><description>Obstructive Sleep Apnea (OSAS) is a disease associated with the increase of cardiovascular risk and it is characterized by repeated episodes of Intermittent Hypoxia (IH) which inducing oxidative stress and systemic inflammation. Mitochondria are cell organelles involved in the respiratory that have their own DNA (MtDNA). The aim of this study was to investigate if the increase of oxidative stress in OSAS patients can induce also MtDNA alterations.
46 OSAS patients (age 59.27 ± 11.38; BMI 30.84 ± 3.64; AHI 36.63 ± 24.18) were compared with 36 control subjects (age 54.42 ± 6.63; BMI 29.06 ± 4.7; AHI 3.8 ± 1.10). In blood cells Content of MtDNA and nuclear DNA (nDNA) was measured in OSAS patients by Real Time PCR. The ratio between MtDNA/nDNA was then calculated. Presence of oxidative stress was evaluated by levels of Reactive Oxygen Metabolites (ROMs), measured by diacron reactive oxygen metabolite test (d-ROM test).
MtDNA/nDNA was higher in patients with OSAS than in the control group (150.94 ± 49.14 vs 128.96 ± 45.8; p = 0.04), the levels of ROMs were also higher in OSAS subjects (329.71 ± 70.17 vs 226 ± 36.76; p = 0.04) and they were positively correlated with MtDNA/nDNA (R = 0.5, p < 0.01).
In OSAS patients there is a Mitochondrial DNA damage induced by the increase of oxidative stress. Intermittent hypoxia seems to be the main mechanism which leads to this process.</description><subject>Aged</subject><subject>Cardiovascular diseases</subject><subject>Case-Control Studies</subject><subject>DNA Damage</subject><subject>DNA, Mitochondrial - blood</subject><subject>DNA, Mitochondrial - genetics</subject><subject>Female</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Oxidative Stress</subject><subject>Reactive Oxygen Species - blood</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Risk factors</subject><subject>Sleep Apnea, Obstructive - blood</subject><subject>Sleep Apnea, Obstructive - genetics</subject><issn>1465-993X</issn><issn>1465-9921</issn><issn>1465-993X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUtLAzEYRYMo1tcPcCMDbtyM5jFJJhuh-AYfGwV3IZP5po1Mk5pMBf-9KVWpIFkkJOdcvnAROiT4lJBanCVCFSMlJrzEFPNSbqAdUgleKsVeN9fOI7Sb0hvGRNaSb6MR5bXClIodJB_cEOw0-DY60xeXj-PC9ANEM7jgC-eL0KQhLuzgPqBIPcC8MHMPZh9tdaZPcPC976GX66vni9vy_unm7mJ8X9pKiKGUitJatJg3XdMaaKDmvG1qZjqJWcWExdJaC0pQa4WgpiaKC5AdZKVjvGZ76HyVO180M2gt-CGaXs-jm5n4qYNx-u-Ld1M9CR-6YooKugw4-Q6I4X0BadAzlyz0vfEQFkkTISuhcIVpRo9X6MT0oJ3vQk60S1yPeUWqPLIUmTr9h8qrhZmzwUPn8v0fgawEG0NKEbrf6QnWyx71qkede9TLHrXMztH6t3-Nn-LYF_rkmEI</recordid><startdate>20150407</startdate><enddate>20150407</enddate><creator>Lacedonia, Donato</creator><creator>Carpagnano, Giovanna E</creator><creator>Crisetti, Elisabetta</creator><creator>Cotugno, Grazia</creator><creator>Palladino, Grazia P</creator><creator>Patricelli, Giulia</creator><creator>Sabato, Roberto</creator><creator>Foschino Barbaro, Maria P</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150407</creationdate><title>Mitochondrial DNA alteration in obstructive sleep apnea</title><author>Lacedonia, Donato ; Carpagnano, Giovanna E ; Crisetti, Elisabetta ; Cotugno, Grazia ; Palladino, Grazia P ; Patricelli, Giulia ; Sabato, Roberto ; Foschino Barbaro, Maria P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-792286d05bfbdaebe855db83af703436c07ccce962cc662a81956e7fed05f3583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aged</topic><topic>Cardiovascular diseases</topic><topic>Case-Control Studies</topic><topic>DNA Damage</topic><topic>DNA, Mitochondrial - blood</topic><topic>DNA, Mitochondrial - genetics</topic><topic>Female</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Oxidative Stress</topic><topic>Reactive Oxygen Species - blood</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Risk factors</topic><topic>Sleep Apnea, Obstructive - blood</topic><topic>Sleep Apnea, Obstructive - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lacedonia, Donato</creatorcontrib><creatorcontrib>Carpagnano, Giovanna E</creatorcontrib><creatorcontrib>Crisetti, Elisabetta</creatorcontrib><creatorcontrib>Cotugno, Grazia</creatorcontrib><creatorcontrib>Palladino, Grazia P</creatorcontrib><creatorcontrib>Patricelli, Giulia</creatorcontrib><creatorcontrib>Sabato, Roberto</creatorcontrib><creatorcontrib>Foschino Barbaro, Maria P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Respiratory research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lacedonia, Donato</au><au>Carpagnano, Giovanna E</au><au>Crisetti, Elisabetta</au><au>Cotugno, Grazia</au><au>Palladino, Grazia P</au><au>Patricelli, Giulia</au><au>Sabato, Roberto</au><au>Foschino Barbaro, Maria P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mitochondrial DNA alteration in obstructive sleep apnea</atitle><jtitle>Respiratory research</jtitle><addtitle>Respir Res</addtitle><date>2015-04-07</date><risdate>2015</risdate><volume>16</volume><issue>1</issue><spage>47</spage><epage>47</epage><pages>47-47</pages><artnum>47</artnum><issn>1465-993X</issn><issn>1465-9921</issn><eissn>1465-993X</eissn><abstract>Obstructive Sleep Apnea (OSAS) is a disease associated with the increase of cardiovascular risk and it is characterized by repeated episodes of Intermittent Hypoxia (IH) which inducing oxidative stress and systemic inflammation. Mitochondria are cell organelles involved in the respiratory that have their own DNA (MtDNA). The aim of this study was to investigate if the increase of oxidative stress in OSAS patients can induce also MtDNA alterations.
46 OSAS patients (age 59.27 ± 11.38; BMI 30.84 ± 3.64; AHI 36.63 ± 24.18) were compared with 36 control subjects (age 54.42 ± 6.63; BMI 29.06 ± 4.7; AHI 3.8 ± 1.10). In blood cells Content of MtDNA and nuclear DNA (nDNA) was measured in OSAS patients by Real Time PCR. The ratio between MtDNA/nDNA was then calculated. Presence of oxidative stress was evaluated by levels of Reactive Oxygen Metabolites (ROMs), measured by diacron reactive oxygen metabolite test (d-ROM test).
MtDNA/nDNA was higher in patients with OSAS than in the control group (150.94 ± 49.14 vs 128.96 ± 45.8; p = 0.04), the levels of ROMs were also higher in OSAS subjects (329.71 ± 70.17 vs 226 ± 36.76; p = 0.04) and they were positively correlated with MtDNA/nDNA (R = 0.5, p < 0.01).
In OSAS patients there is a Mitochondrial DNA damage induced by the increase of oxidative stress. Intermittent hypoxia seems to be the main mechanism which leads to this process.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>25890226</pmid><doi>10.1186/s12931-015-0205-7</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Cardiovascular diseases Case-Control Studies DNA Damage DNA, Mitochondrial - blood DNA, Mitochondrial - genetics Female Genetic aspects Health aspects Humans Male Middle Aged Oxidative Stress Reactive Oxygen Species - blood Real-Time Polymerase Chain Reaction Risk factors Sleep Apnea, Obstructive - blood Sleep Apnea, Obstructive - genetics |
| title | Mitochondrial DNA alteration in obstructive sleep apnea |
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