Development of a real-time PCR Assay for identification of Coccidioides immitis by use of the BD Max system

Rapid real-time PCR (RT-PCR) can be performed in a community hospital setting to identify Coccidioides species using the new Becton Dickinson molecular instrument BD Max. Following sample preparation, DNA extraction and PCR were performed on the BD Max using the BD Max extraction kit ExK-DNA-1 test...

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Veröffentlicht in:	Journal of clinical microbiology 2015-03, Vol.53 (3), p.926-929
Hauptverfasser:	Mitchell, Marilyn, Dizon, Dominic, Libke, Robert, Peterson, Michael, Slater, David, Dhillon, Akashdeep
Format:	Artikel
Sprache:	eng
Schlagworte:	Body Fluids - microbiology Coccidioides - genetics Coccidioides - isolation & purification Coccidioidomycosis - diagnosis Humans Molecular Diagnostic Techniques - methods Mycology Real-Time Polymerase Chain Reaction - methods Sensitivity and Specificity Time Factors
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