Antileishmanial and Cytotoxic Effects of Essential Oil and Methanolic Extract of Myrtus communis L
Plants used for traditional medicine contain a wide range of substances that can be used to treat various diseases such as infectious diseases. The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tro...
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| Veröffentlicht in: | Korean journal of parasitology 2015-02, Vol.53 (1), p.21-27 |
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| creator | Mahmoudvand, H., Kerman University of Medical Sciences, Kerman, Iran Ezzatkhah, F., Kerman University of Medical Sciences, Kerman, Iran Sharififar, F., Kerman University of Medical Sciences, Kerman, Iran Sharifi, I., Kerman University of Medical Sciences, Kerman, Iran Dezaki, E.S., Kerman University of Medical Sciences, Kerman, Iran |
| description | Plants used for traditional medicine contain a wide range of substances that can be used to treat various diseases such as infectious diseases. The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model. Antileishmanial effects of essential oil and methanolic extract of M. communis on promastigote forms and their cytotoxic activities against J774 cells were evaluated using MTT assay for 72 hr. In addition, their leishmanicidal activity against amastigote forms was determined in a macrophage model, for 72 hr. Findings showed that the main components of essential oil were alpha-pinene (24.7%), 1,8-cineole (19.6%), and linalool (12.6%). Findings demonstrated that M. communis, particularly its essential oil, significantly (P0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response. The IC50 values for essential oil and methanolic extract was 8.4 and 28.9 microgram/ml against promastigotes, respectively. These values were 11.6 and 40.8 microgram/ml against amastigote forms, respectively. Glucantime as control drug also revealed IC50 values of 88.3 and 44.6 microgram/ml for promastigotes and amastigotes of L. tropica, respectively. The in vitro assay demonstrated no significant cytotoxicity in J774 cells. However, essential oil indicated a more cytotoxic effect as compared with the methanolic extract of M. communis. The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent. |
| doi_str_mv | 10.3347/kjp.2015.53.1.21 |
| format | Article |
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The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model. Antileishmanial effects of essential oil and methanolic extract of M. communis on promastigote forms and their cytotoxic activities against J774 cells were evaluated using MTT assay for 72 hr. In addition, their leishmanicidal activity against amastigote forms was determined in a macrophage model, for 72 hr. Findings showed that the main components of essential oil were alpha-pinene (24.7%), 1,8-cineole (19.6%), and linalool (12.6%). Findings demonstrated that M. communis, particularly its essential oil, significantly (P0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response. The IC50 values for essential oil and methanolic extract was 8.4 and 28.9 microgram/ml against promastigotes, respectively. These values were 11.6 and 40.8 microgram/ml against amastigote forms, respectively. Glucantime as control drug also revealed IC50 values of 88.3 and 44.6 microgram/ml for promastigotes and amastigotes of L. tropica, respectively. The in vitro assay demonstrated no significant cytotoxicity in J774 cells. However, essential oil indicated a more cytotoxic effect as compared with the methanolic extract of M. communis. The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent.</description><identifier>ISSN: 0023-4001</identifier><identifier>EISSN: 1738-0006</identifier><identifier>DOI: 10.3347/kjp.2015.53.1.21</identifier><identifier>PMID: 25748705</identifier><language>eng</language><publisher>Korea (South): 대한기생충학열대의학회</publisher><subject>Animals ; Antiprotozoal Agents - isolation & purification ; Antiprotozoal Agents - pharmacology ; Antiprotozoal Agents - toxicity ; Cell Line ; Cell Survival - drug effects ; cutaneous leishmaniasis,promastigote,amastigote,J774-A1 cell ; Cyclohexanols - isolation & purification ; Cyclohexanols - pharmacology ; Cyclohexanols - toxicity ; Inhibitory Concentration 50 ; LEISHMANIA TROPICA ; Leishmania tropica - drug effects ; Leishmania tropica - physiology ; Macrophages - drug effects ; Mice ; Monoterpenes - isolation & purification ; Monoterpenes - pharmacology ; Monoterpenes - toxicity ; Myrtus - chemistry ; MYRTUS COMMUNIS ; Oils, Volatile - isolation & purification ; Oils, Volatile - pharmacology ; Oils, Volatile - toxicity ; Original ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Plant Extracts - toxicity</subject><ispartof>Korean journal of parasitology, 2015-02, Vol.53 (1), p.21-27</ispartof><rights>2015, Korean Society for Parasitology and Tropical Medicine 2015</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-ae7c2bab223db3fce0c3e9ded357b1d9e5ab23714c94208e2368db2cbf2d970c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384785/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384785/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25748705$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mahmoudvand, H., Kerman University of Medical Sciences, Kerman, Iran</creatorcontrib><creatorcontrib>Ezzatkhah, F., Kerman University of Medical Sciences, Kerman, Iran</creatorcontrib><creatorcontrib>Sharififar, F., Kerman University of Medical Sciences, Kerman, Iran</creatorcontrib><creatorcontrib>Sharifi, I., Kerman University of Medical Sciences, Kerman, Iran</creatorcontrib><creatorcontrib>Dezaki, E.S., Kerman University of Medical Sciences, Kerman, Iran</creatorcontrib><title>Antileishmanial and Cytotoxic Effects of Essential Oil and Methanolic Extract of Myrtus communis L</title><title>Korean journal of parasitology</title><addtitle>Korean J Parasitol</addtitle><description>Plants used for traditional medicine contain a wide range of substances that can be used to treat various diseases such as infectious diseases. The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model. Antileishmanial effects of essential oil and methanolic extract of M. communis on promastigote forms and their cytotoxic activities against J774 cells were evaluated using MTT assay for 72 hr. In addition, their leishmanicidal activity against amastigote forms was determined in a macrophage model, for 72 hr. Findings showed that the main components of essential oil were alpha-pinene (24.7%), 1,8-cineole (19.6%), and linalool (12.6%). Findings demonstrated that M. communis, particularly its essential oil, significantly (P0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response. The IC50 values for essential oil and methanolic extract was 8.4 and 28.9 microgram/ml against promastigotes, respectively. These values were 11.6 and 40.8 microgram/ml against amastigote forms, respectively. Glucantime as control drug also revealed IC50 values of 88.3 and 44.6 microgram/ml for promastigotes and amastigotes of L. tropica, respectively. The in vitro assay demonstrated no significant cytotoxicity in J774 cells. However, essential oil indicated a more cytotoxic effect as compared with the methanolic extract of M. communis. The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent.</description><subject>Animals</subject><subject>Antiprotozoal Agents - isolation & purification</subject><subject>Antiprotozoal Agents - pharmacology</subject><subject>Antiprotozoal Agents - toxicity</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>cutaneous leishmaniasis,promastigote,amastigote,J774-A1 cell</subject><subject>Cyclohexanols - isolation & purification</subject><subject>Cyclohexanols - pharmacology</subject><subject>Cyclohexanols - toxicity</subject><subject>Inhibitory Concentration 50</subject><subject>LEISHMANIA TROPICA</subject><subject>Leishmania tropica - drug effects</subject><subject>Leishmania tropica - physiology</subject><subject>Macrophages - drug effects</subject><subject>Mice</subject><subject>Monoterpenes - isolation & purification</subject><subject>Monoterpenes - pharmacology</subject><subject>Monoterpenes - toxicity</subject><subject>Myrtus - chemistry</subject><subject>MYRTUS COMMUNIS</subject><subject>Oils, Volatile - isolation & purification</subject><subject>Oils, Volatile - pharmacology</subject><subject>Oils, Volatile - toxicity</subject><subject>Original</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Extracts - toxicity</subject><issn>0023-4001</issn><issn>1738-0006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpV0c9v0zAUB3ALMbGycd9lKBckLgnPPxInF6SplA2tWyUEZ8uxndUjiTvbQet_j6uMCk7v8D7vh_RF6AJDQSnjn3497goCuCxKWuCC4FdogTmtcwCoXqMFAKE5A8Cn6G0IjwCUlBy_QaepsJpDuUDt1Rhtb2zYDnK0ss_kqLPlPrronq3KVl1nVAyZ67JVCCbZRDZ2ZncmbuXo-oN7jl6qeHB3ex-nkCk3DNNoQ7Y-Ryed7IN591LP0M-vqx_Lm3y9uf62vFrnikEVc2m4Iq1sCaG6pZ0yoKhptNG05C3WjSlTj3LMVMMI1IbQqtYtUW1HdMMTPkOf5727qR2MVulbL3ux83aQfi-ctOL_zmi34sH9FozWjNdlWvDxZYF3T5MJUQw2KNP3cjRuCgJXFW4a3NSQKMxUeReCN93xDAZxiEakaMQhGlFSgQXBaeT9v-8dB_5mkcCHGYxTahlt5dHcb76soCIE0urkLmfXSSfkg7dB3H5Pp6oUNAVG_wBIj6Iw</recordid><startdate>20150201</startdate><enddate>20150201</enddate><creator>Mahmoudvand, H., Kerman University of Medical Sciences, Kerman, Iran</creator><creator>Ezzatkhah, F., Kerman University of Medical Sciences, Kerman, Iran</creator><creator>Sharififar, F., Kerman University of Medical Sciences, Kerman, Iran</creator><creator>Sharifi, I., Kerman University of Medical Sciences, Kerman, Iran</creator><creator>Dezaki, E.S., Kerman University of Medical Sciences, Kerman, Iran</creator><general>대한기생충학열대의학회</general><general>The Korean Society for Parasitology and Tropical Medicine</general><scope>FBQ</scope><scope>DBRKI</scope><scope>TDB</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150201</creationdate><title>Antileishmanial and Cytotoxic Effects of Essential Oil and Methanolic Extract of Myrtus communis L</title><author>Mahmoudvand, H., Kerman University of Medical Sciences, Kerman, Iran ; Ezzatkhah, F., Kerman University of Medical Sciences, Kerman, Iran ; Sharififar, F., Kerman University of Medical Sciences, Kerman, Iran ; Sharifi, I., Kerman University of Medical Sciences, Kerman, Iran ; Dezaki, E.S., Kerman University of Medical Sciences, Kerman, Iran</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-ae7c2bab223db3fce0c3e9ded357b1d9e5ab23714c94208e2368db2cbf2d970c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Antiprotozoal Agents - isolation & purification</topic><topic>Antiprotozoal Agents - pharmacology</topic><topic>Antiprotozoal Agents - toxicity</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>cutaneous leishmaniasis,promastigote,amastigote,J774-A1 cell</topic><topic>Cyclohexanols - isolation & purification</topic><topic>Cyclohexanols - pharmacology</topic><topic>Cyclohexanols - toxicity</topic><topic>Inhibitory Concentration 50</topic><topic>LEISHMANIA TROPICA</topic><topic>Leishmania tropica - drug effects</topic><topic>Leishmania tropica - physiology</topic><topic>Macrophages - drug effects</topic><topic>Mice</topic><topic>Monoterpenes - isolation & purification</topic><topic>Monoterpenes - pharmacology</topic><topic>Monoterpenes - toxicity</topic><topic>Myrtus - chemistry</topic><topic>MYRTUS COMMUNIS</topic><topic>Oils, Volatile - isolation & purification</topic><topic>Oils, Volatile - pharmacology</topic><topic>Oils, Volatile - toxicity</topic><topic>Original</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Extracts - toxicity</topic><toplevel>online_resources</toplevel><creatorcontrib>Mahmoudvand, H., Kerman University of Medical Sciences, Kerman, Iran</creatorcontrib><creatorcontrib>Ezzatkhah, F., Kerman University of Medical Sciences, Kerman, Iran</creatorcontrib><creatorcontrib>Sharififar, F., Kerman University of Medical Sciences, Kerman, Iran</creatorcontrib><creatorcontrib>Sharifi, I., Kerman University of Medical Sciences, Kerman, Iran</creatorcontrib><creatorcontrib>Dezaki, E.S., Kerman University of Medical Sciences, Kerman, Iran</creatorcontrib><collection>AGRIS</collection><collection>DBPIA - 디비피아</collection><collection>DBPIA</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Korean journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mahmoudvand, H., Kerman University of Medical Sciences, Kerman, Iran</au><au>Ezzatkhah, F., Kerman University of Medical Sciences, Kerman, Iran</au><au>Sharififar, F., Kerman University of Medical Sciences, Kerman, Iran</au><au>Sharifi, I., Kerman University of Medical Sciences, Kerman, Iran</au><au>Dezaki, E.S., Kerman University of Medical Sciences, Kerman, Iran</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antileishmanial and Cytotoxic Effects of Essential Oil and Methanolic Extract of Myrtus communis L</atitle><jtitle>Korean journal of parasitology</jtitle><addtitle>Korean J Parasitol</addtitle><date>2015-02-01</date><risdate>2015</risdate><volume>53</volume><issue>1</issue><spage>21</spage><epage>27</epage><pages>21-27</pages><issn>0023-4001</issn><eissn>1738-0006</eissn><abstract>Plants used for traditional medicine contain a wide range of substances that can be used to treat various diseases such as infectious diseases. The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model. Antileishmanial effects of essential oil and methanolic extract of M. communis on promastigote forms and their cytotoxic activities against J774 cells were evaluated using MTT assay for 72 hr. In addition, their leishmanicidal activity against amastigote forms was determined in a macrophage model, for 72 hr. Findings showed that the main components of essential oil were alpha-pinene (24.7%), 1,8-cineole (19.6%), and linalool (12.6%). Findings demonstrated that M. communis, particularly its essential oil, significantly (P0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response. The IC50 values for essential oil and methanolic extract was 8.4 and 28.9 microgram/ml against promastigotes, respectively. These values were 11.6 and 40.8 microgram/ml against amastigote forms, respectively. Glucantime as control drug also revealed IC50 values of 88.3 and 44.6 microgram/ml for promastigotes and amastigotes of L. tropica, respectively. The in vitro assay demonstrated no significant cytotoxicity in J774 cells. However, essential oil indicated a more cytotoxic effect as compared with the methanolic extract of M. communis. The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent.</abstract><cop>Korea (South)</cop><pub>대한기생충학열대의학회</pub><pmid>25748705</pmid><doi>10.3347/kjp.2015.53.1.21</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; KoreaMed Open Access; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Antiprotozoal Agents - isolation & purification Antiprotozoal Agents - pharmacology Antiprotozoal Agents - toxicity Cell Line Cell Survival - drug effects cutaneous leishmaniasis,promastigote,amastigote,J774-A1 cell Cyclohexanols - isolation & purification Cyclohexanols - pharmacology Cyclohexanols - toxicity Inhibitory Concentration 50 LEISHMANIA TROPICA Leishmania tropica - drug effects Leishmania tropica - physiology Macrophages - drug effects Mice Monoterpenes - isolation & purification Monoterpenes - pharmacology Monoterpenes - toxicity Myrtus - chemistry MYRTUS COMMUNIS Oils, Volatile - isolation & purification Oils, Volatile - pharmacology Oils, Volatile - toxicity Original Plant Extracts - isolation & purification Plant Extracts - pharmacology Plant Extracts - toxicity |
| title | Antileishmanial and Cytotoxic Effects of Essential Oil and Methanolic Extract of Myrtus communis L |
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