Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis

Genetic screens are powerful tools for identifying genes responsible for diverse phenotypes. Here we describe a genome-wide CRISPR/Cas9-mediated loss-of-function screen in tumor growth and metastasis. We mutagenized a non-metastatic mouse cancer cell line using a genome-scale library with 67,405 sin...

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Veröffentlicht in:	Cell 2015-03, Vol.160 (6), p.1246-1260
Hauptverfasser:	Chen, Sidi, Sanjana, Neville E., Zheng, Kaijie, Shalem, Ophir, Lee, Kyungheon, Shi, Xi, Scott, David A., Song, Jun, Pan, Jen Q., Weissleder, Ralph, Lee, Hakho, Zhang, Feng, Sharp, Phillip A.
Format:	Artikel
Sprache:	eng
Schlagworte:	animal models Animals Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - pathology CRISPR-Cas Systems evolution Gene Knockout Techniques genes Genome-Wide Association Study Humans loss-of-function mutation Lung Neoplasms - genetics Lung Neoplasms - pathology metastasis Mice mutants Neoplasm Metastasis - genetics neoplasms phenotype RNA RNA, Guide, CRISPR-Cas Systems screening
Online-Zugang:	Volltext
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