Room-Temperature Distance Measurements of Immobilized Spin-Labeled Protein by DEER/PELDOR

Nitroxide spin labels are used for double electron-electron resonance (DEER) measurements of distances between sites in biomolecules. Rotation of gem-dimethyls in commonly used nitroxides causes spin echo dephasing times (Tm) to be too short to perform DEER measurements at temperatures between ∼80 a...

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Veröffentlicht in:	Biophysical journal 2015-03, Vol.108 (5), p.1213-1219
Hauptverfasser:	Meyer, Virginia, Swanson, Michael A., Clouston, Laura J., Boratyński, Przemysław J., Stein, Richard A., Mchaourab, Hassane S., Rajca, Andrzej, Eaton, Sandra S., Eaton, Gareth R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Electron Spin Resonance Spectroscopy - methods Electrons Enzymes, Immobilized - chemistry Glycerol Matrix Measurement Molecules Muramidase - chemistry Proteins and Nucleic Acids Spin Labels Temperature Viral Proteins - chemistry
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