Effect of calcitriol supplementation and tail suspension on serum biomarkers of bone formation in rats
Background Calcitriol is documented to cause significant increase in bone mass densitometry counteracting osteoporosis. Promising results of calcitriol supplementation in studies aiming space flight induced osteoporosis is little and the effect of this hormone on biomarkers of bone metabolism is not...
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| Veröffentlicht in: | Journal of diabetes and metabolic disorders 2015-03, Vol.14 (1), p.14-14, Article 14 |
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| creator | Hashemian, Seyed Jafar Rismanchi, Mojtaba Esfahani, Ensiyeh Nasli Khoshvaghti, Amir Razi, Farideh |
| description | Background
Calcitriol is documented to cause significant increase in bone mass densitometry counteracting osteoporosis. Promising results of calcitriol supplementation in studies aiming space flight induced osteoporosis is little and the effect of this hormone on biomarkers of bone metabolism is not examined yet in space flight models of osteoporosis in rats.
Methods
This was an interventional animal study being performed in a 1-month period. We included 21 Sprague Dawley strain rats (>200 gr, >6 week) who were randomly assigned to receive daily supplementation of oral 0.03μgr calcitriol and to be submitted to tail suspension model. Rats were followed for 1 month and were tested for serum osteocalcin (OC), alkaline phosphatase (ALP) and serum calcium at the beginning and the end of the study period. The results were analyzed and compared between groups.
Results
Although serum levels of osteocalcin and alkaline phosphatase biomarkers and total serum calcium were not significantly different within and between study groups, their levels were increased in tail suspension model compared to control group. The levels of these biomarkers were lower in those who were submitted to tail suspension model and received calcitriol supplementation compared to those who were only submitted to tail suspension (
60.14 ± 11.73 ng/mL vs. 58.29 ± 2.69 ng/mL; p = 0.696 for osteocalcin and 381.86 ± 99.16 mU/mL vs.
362.57 ± 27.41
ng/mL; p = 0.635 for alkaline phosphatase
).
Conclusion
Supplementation of daily diet with calcitriol in rats under weightlessness conditions may results in lower values for bone metabolic biomarkers of alkaline phosphatase and osteocalcin and serum calcium. This pattern of change in biomarkers of bone formation, may point to the capacity of calcitriol supplementation in preventing cellular process of osteoporosis. Thus calcitriol supplementation could be an available, economic and effective strategy for preventing bone metabolic changes related to weightlessness commonly encountered in space flight. The outcome of this study needs to be further studied in future trying to find more definite results. |
| doi_str_mv | 10.1186/s40200-015-0142-5 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4371718</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A541669047</galeid><sourcerecordid>A541669047</sourcerecordid><originalsourceid>FETCH-LOGICAL-b601t-37e102921c4d876f70eecf10e7c48936323976c676a41072b03cd5e68d8bd0973</originalsourceid><addsrcrecordid>eNp9kl9r3SAYxmVsrKXrB9jNCAzGbtKpSdTcDErX_YHCbrZrMeb1HDujmSaFffsp6co5cFhMiPj-3gcfHxF6TfAVIYJ9SC2mGNeYdPlrad09Q-eUdqRmnSDPD-Zn6DKle5wfzoUg7CU6o53ArGH4HJlbY0AvVTCVVk7bJdrgqrTOs4MJ_KIWG3yl_FgtypZCmsGnspbfBHGdqsGGScVfEFNRGYKHyoQ4bZ3WV1Et6RV6YZRLcPn4v0A_P9_-uPla333_8u3m-q4eGCZL3XAgmPaU6HYUnBmOAbQhGLhuRd-whjY9Z5pxplqCOR1wo8cOmBjFMOKeNxfo46Y7r8MEo84OonJyjjZv8Y8Mysrjird7uQsPsm044URkgU-bQHF1WuC4osMktyhkjkKWKGSXZd4_7iOG3yukRU42aXBOeQhrkoQx1vdY9H1G327oTjmQ1puQdXXB5XXXZrDHbTF2dYLKY4TJ6nzmxub1o4Z3Bw17UG7Zp-DWkko6BskG6hhSimCezBIsy007ae_N4TE_dfy7VxmgG5Byye8gyvuwRp-j_4_qX2fp3fU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1666990899</pqid></control><display><type>article</type><title>Effect of calcitriol supplementation and tail suspension on serum biomarkers of bone formation in rats</title><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>SpringerLink Journals - AutoHoldings</source><creator>Hashemian, Seyed Jafar ; Rismanchi, Mojtaba ; Esfahani, Ensiyeh Nasli ; Khoshvaghti, Amir ; Razi, Farideh</creator><creatorcontrib>Hashemian, Seyed Jafar ; Rismanchi, Mojtaba ; Esfahani, Ensiyeh Nasli ; Khoshvaghti, Amir ; Razi, Farideh</creatorcontrib><description>Background
Calcitriol is documented to cause significant increase in bone mass densitometry counteracting osteoporosis. Promising results of calcitriol supplementation in studies aiming space flight induced osteoporosis is little and the effect of this hormone on biomarkers of bone metabolism is not examined yet in space flight models of osteoporosis in rats.
Methods
This was an interventional animal study being performed in a 1-month period. We included 21 Sprague Dawley strain rats (>200 gr, >6 week) who were randomly assigned to receive daily supplementation of oral 0.03μgr calcitriol and to be submitted to tail suspension model. Rats were followed for 1 month and were tested for serum osteocalcin (OC), alkaline phosphatase (ALP) and serum calcium at the beginning and the end of the study period. The results were analyzed and compared between groups.
Results
Although serum levels of osteocalcin and alkaline phosphatase biomarkers and total serum calcium were not significantly different within and between study groups, their levels were increased in tail suspension model compared to control group. The levels of these biomarkers were lower in those who were submitted to tail suspension model and received calcitriol supplementation compared to those who were only submitted to tail suspension (
60.14 ± 11.73 ng/mL vs. 58.29 ± 2.69 ng/mL; p = 0.696 for osteocalcin and 381.86 ± 99.16 mU/mL vs.
362.57 ± 27.41
ng/mL; p = 0.635 for alkaline phosphatase
).
Conclusion
Supplementation of daily diet with calcitriol in rats under weightlessness conditions may results in lower values for bone metabolic biomarkers of alkaline phosphatase and osteocalcin and serum calcium. This pattern of change in biomarkers of bone formation, may point to the capacity of calcitriol supplementation in preventing cellular process of osteoporosis. Thus calcitriol supplementation could be an available, economic and effective strategy for preventing bone metabolic changes related to weightlessness commonly encountered in space flight. The outcome of this study needs to be further studied in future trying to find more definite results.</description><identifier>ISSN: 2251-6581</identifier><identifier>EISSN: 2251-6581</identifier><identifier>DOI: 10.1186/s40200-015-0142-5</identifier><identifier>PMID: 25806360</identifier><language>eng</language><publisher>London: BioMed Central</publisher><subject>Alfacalcidol ; Biological markers ; Bones ; Calcifediol ; Density ; Diabetes ; Endocrinology ; Health aspects ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Osteoporosis ; Phosphatases ; Physiological aspects ; Research Article ; Risk factors ; Vitamin D</subject><ispartof>Journal of diabetes and metabolic disorders, 2015-03, Vol.14 (1), p.14-14, Article 14</ispartof><rights>Hashemian et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated.</rights><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Hashemian et al.; licensee BioMed Central. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b601t-37e102921c4d876f70eecf10e7c48936323976c676a41072b03cd5e68d8bd0973</citedby><cites>FETCH-LOGICAL-b601t-37e102921c4d876f70eecf10e7c48936323976c676a41072b03cd5e68d8bd0973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371718/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371718/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27906,27907,41470,42539,51301,53773,53775</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25806360$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hashemian, Seyed Jafar</creatorcontrib><creatorcontrib>Rismanchi, Mojtaba</creatorcontrib><creatorcontrib>Esfahani, Ensiyeh Nasli</creatorcontrib><creatorcontrib>Khoshvaghti, Amir</creatorcontrib><creatorcontrib>Razi, Farideh</creatorcontrib><title>Effect of calcitriol supplementation and tail suspension on serum biomarkers of bone formation in rats</title><title>Journal of diabetes and metabolic disorders</title><addtitle>J Diabetes Metab Disord</addtitle><addtitle>J Diabetes Metab Disord</addtitle><description>Background
Calcitriol is documented to cause significant increase in bone mass densitometry counteracting osteoporosis. Promising results of calcitriol supplementation in studies aiming space flight induced osteoporosis is little and the effect of this hormone on biomarkers of bone metabolism is not examined yet in space flight models of osteoporosis in rats.
Methods
This was an interventional animal study being performed in a 1-month period. We included 21 Sprague Dawley strain rats (>200 gr, >6 week) who were randomly assigned to receive daily supplementation of oral 0.03μgr calcitriol and to be submitted to tail suspension model. Rats were followed for 1 month and were tested for serum osteocalcin (OC), alkaline phosphatase (ALP) and serum calcium at the beginning and the end of the study period. The results were analyzed and compared between groups.
Results
Although serum levels of osteocalcin and alkaline phosphatase biomarkers and total serum calcium were not significantly different within and between study groups, their levels were increased in tail suspension model compared to control group. The levels of these biomarkers were lower in those who were submitted to tail suspension model and received calcitriol supplementation compared to those who were only submitted to tail suspension (
60.14 ± 11.73 ng/mL vs. 58.29 ± 2.69 ng/mL; p = 0.696 for osteocalcin and 381.86 ± 99.16 mU/mL vs.
362.57 ± 27.41
ng/mL; p = 0.635 for alkaline phosphatase
).
Conclusion
Supplementation of daily diet with calcitriol in rats under weightlessness conditions may results in lower values for bone metabolic biomarkers of alkaline phosphatase and osteocalcin and serum calcium. This pattern of change in biomarkers of bone formation, may point to the capacity of calcitriol supplementation in preventing cellular process of osteoporosis. Thus calcitriol supplementation could be an available, economic and effective strategy for preventing bone metabolic changes related to weightlessness commonly encountered in space flight. The outcome of this study needs to be further studied in future trying to find more definite results.</description><subject>Alfacalcidol</subject><subject>Biological markers</subject><subject>Bones</subject><subject>Calcifediol</subject><subject>Density</subject><subject>Diabetes</subject><subject>Endocrinology</subject><subject>Health aspects</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>Osteoporosis</subject><subject>Phosphatases</subject><subject>Physiological aspects</subject><subject>Research Article</subject><subject>Risk factors</subject><subject>Vitamin D</subject><issn>2251-6581</issn><issn>2251-6581</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kl9r3SAYxmVsrKXrB9jNCAzGbtKpSdTcDErX_YHCbrZrMeb1HDujmSaFffsp6co5cFhMiPj-3gcfHxF6TfAVIYJ9SC2mGNeYdPlrad09Q-eUdqRmnSDPD-Zn6DKle5wfzoUg7CU6o53ArGH4HJlbY0AvVTCVVk7bJdrgqrTOs4MJ_KIWG3yl_FgtypZCmsGnspbfBHGdqsGGScVfEFNRGYKHyoQ4bZ3WV1Et6RV6YZRLcPn4v0A_P9_-uPla333_8u3m-q4eGCZL3XAgmPaU6HYUnBmOAbQhGLhuRd-whjY9Z5pxplqCOR1wo8cOmBjFMOKeNxfo46Y7r8MEo84OonJyjjZv8Y8Mysrjird7uQsPsm044URkgU-bQHF1WuC4osMktyhkjkKWKGSXZd4_7iOG3yukRU42aXBOeQhrkoQx1vdY9H1G327oTjmQ1puQdXXB5XXXZrDHbTF2dYLKY4TJ6nzmxub1o4Z3Bw17UG7Zp-DWkko6BskG6hhSimCezBIsy007ae_N4TE_dfy7VxmgG5Byye8gyvuwRp-j_4_qX2fp3fU</recordid><startdate>20150319</startdate><enddate>20150319</enddate><creator>Hashemian, Seyed Jafar</creator><creator>Rismanchi, Mojtaba</creator><creator>Esfahani, Ensiyeh Nasli</creator><creator>Khoshvaghti, Amir</creator><creator>Razi, Farideh</creator><general>BioMed Central</general><general>BioMed Central Ltd</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150319</creationdate><title>Effect of calcitriol supplementation and tail suspension on serum biomarkers of bone formation in rats</title><author>Hashemian, Seyed Jafar ; Rismanchi, Mojtaba ; Esfahani, Ensiyeh Nasli ; Khoshvaghti, Amir ; Razi, Farideh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b601t-37e102921c4d876f70eecf10e7c48936323976c676a41072b03cd5e68d8bd0973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Alfacalcidol</topic><topic>Biological markers</topic><topic>Bones</topic><topic>Calcifediol</topic><topic>Density</topic><topic>Diabetes</topic><topic>Endocrinology</topic><topic>Health aspects</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>Osteoporosis</topic><topic>Phosphatases</topic><topic>Physiological aspects</topic><topic>Research Article</topic><topic>Risk factors</topic><topic>Vitamin D</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hashemian, Seyed Jafar</creatorcontrib><creatorcontrib>Rismanchi, Mojtaba</creatorcontrib><creatorcontrib>Esfahani, Ensiyeh Nasli</creatorcontrib><creatorcontrib>Khoshvaghti, Amir</creatorcontrib><creatorcontrib>Razi, Farideh</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of diabetes and metabolic disorders</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hashemian, Seyed Jafar</au><au>Rismanchi, Mojtaba</au><au>Esfahani, Ensiyeh Nasli</au><au>Khoshvaghti, Amir</au><au>Razi, Farideh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of calcitriol supplementation and tail suspension on serum biomarkers of bone formation in rats</atitle><jtitle>Journal of diabetes and metabolic disorders</jtitle><stitle>J Diabetes Metab Disord</stitle><addtitle>J Diabetes Metab Disord</addtitle><date>2015-03-19</date><risdate>2015</risdate><volume>14</volume><issue>1</issue><spage>14</spage><epage>14</epage><pages>14-14</pages><artnum>14</artnum><issn>2251-6581</issn><eissn>2251-6581</eissn><abstract>Background
Calcitriol is documented to cause significant increase in bone mass densitometry counteracting osteoporosis. Promising results of calcitriol supplementation in studies aiming space flight induced osteoporosis is little and the effect of this hormone on biomarkers of bone metabolism is not examined yet in space flight models of osteoporosis in rats.
Methods
This was an interventional animal study being performed in a 1-month period. We included 21 Sprague Dawley strain rats (>200 gr, >6 week) who were randomly assigned to receive daily supplementation of oral 0.03μgr calcitriol and to be submitted to tail suspension model. Rats were followed for 1 month and were tested for serum osteocalcin (OC), alkaline phosphatase (ALP) and serum calcium at the beginning and the end of the study period. The results were analyzed and compared between groups.
Results
Although serum levels of osteocalcin and alkaline phosphatase biomarkers and total serum calcium were not significantly different within and between study groups, their levels were increased in tail suspension model compared to control group. The levels of these biomarkers were lower in those who were submitted to tail suspension model and received calcitriol supplementation compared to those who were only submitted to tail suspension (
60.14 ± 11.73 ng/mL vs. 58.29 ± 2.69 ng/mL; p = 0.696 for osteocalcin and 381.86 ± 99.16 mU/mL vs.
362.57 ± 27.41
ng/mL; p = 0.635 for alkaline phosphatase
).
Conclusion
Supplementation of daily diet with calcitriol in rats under weightlessness conditions may results in lower values for bone metabolic biomarkers of alkaline phosphatase and osteocalcin and serum calcium. This pattern of change in biomarkers of bone formation, may point to the capacity of calcitriol supplementation in preventing cellular process of osteoporosis. Thus calcitriol supplementation could be an available, economic and effective strategy for preventing bone metabolic changes related to weightlessness commonly encountered in space flight. The outcome of this study needs to be further studied in future trying to find more definite results.</abstract><cop>London</cop><pub>BioMed Central</pub><pmid>25806360</pmid><doi>10.1186/s40200-015-0142-5</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alfacalcidol Biological markers Bones Calcifediol Density Diabetes Endocrinology Health aspects Medicine Medicine & Public Health Metabolic Diseases Osteoporosis Phosphatases Physiological aspects Research Article Risk factors Vitamin D |
| title | Effect of calcitriol supplementation and tail suspension on serum biomarkers of bone formation in rats |
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