Matrix-assisted laser desorption ionization-time of flight mass spectrometry for differentiation of the dimorphic fungal species Paracoccidioides brasiliensis and Paracoccidioides lutzii
Isolates of Paracoccidioides brasiliensis and Paracoccidioides lutzii, previously characterized by molecular techniques, were identified for the first time by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). All isolates were correctly identified, with log...
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| Veröffentlicht in: | Journal of clinical microbiology 2015-04, Vol.53 (4), p.1383-1386 |
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| container_title | Journal of clinical microbiology |
| container_volume | 53 |
| creator | Nobrega de Almeida, Jr, João Del Negro, Gilda M B Grenfell, Rafaella C Vidal, Monica S M Thomaz, Danilo Y de Figueiredo, Dulce S Y Bagagli, Eduardo Juliano, Luiz Benard, Gil |
| description | Isolates of Paracoccidioides brasiliensis and Paracoccidioides lutzii, previously characterized by molecular techniques, were identified for the first time by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). All isolates were correctly identified, with log score values of >2.0. Thus, MALDI-TOF MS is a new tool for differentiating species of the genus Paracoccidioides. |
| doi_str_mv | 10.1128/JCM.02847-14 |
| format | Article |
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W.</contributor><creatorcontrib>Nobrega de Almeida, Jr, João ; Del Negro, Gilda M B ; Grenfell, Rafaella C ; Vidal, Monica S M ; Thomaz, Danilo Y ; de Figueiredo, Dulce S Y ; Bagagli, Eduardo ; Juliano, Luiz ; Benard, Gil ; Warnock, D. W.</creatorcontrib><description>Isolates of Paracoccidioides brasiliensis and Paracoccidioides lutzii, previously characterized by molecular techniques, were identified for the first time by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). All isolates were correctly identified, with log score values of >2.0. 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All Rights Reserved.</rights><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved. 2015 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-e2cf8fc5194e334f73107b4c3eb87ec5fe572ac1fa4e1168a3ea258a88cbd9013</citedby><cites>FETCH-LOGICAL-c417t-e2cf8fc5194e334f73107b4c3eb87ec5fe572ac1fa4e1168a3ea258a88cbd9013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365217/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365217/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3174,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25631803$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Warnock, D. 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All isolates were correctly identified, with log score values of >2.0. Thus, MALDI-TOF MS is a new tool for differentiating species of the genus Paracoccidioides.</description><subject>DNA, Fungal - genetics</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>HSP70 Heat-Shock Proteins - genetics</subject><subject>Mycology</subject><subject>Paracoccidioides</subject><subject>Paracoccidioides - classification</subject><subject>Paracoccidioides - genetics</subject><subject>Paracoccidioides brasiliensis</subject><subject>Polymerase Chain Reaction - methods</subject><subject>RNA, Fungal - genetics</subject><subject>Species Specificity</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk9v1DAQxS0Eokvhxhn5yIEUT2wn2QsSWvFXreAAEjfLcca7g5J4sR1E-9H4dHi3pQJx4WDZGv_emxnpMfYYxBlA3T1_v7k4E3Wn2grUHbYCse6qphFf7rKVEGtdAcj2hD1I6asQoJTW99lJrRsJnZAr9vPC5kg_KpsSpYwDH23CyAdMIe4zhZmXQ1f28KwyTciD536k7S7zqYh42qPLMUyY4yX3oUjJe4w4ZzqKDnzeYSlPxXFHjvtl3trxKCRM_KON1gXnaKBApS_vo000Es5lIm7n4V9iXPIV0UN2z9sx4aOb-5R9fv3q0-Ztdf7hzbvNy_PKKWhzhbXznXca1gqlVL6VINpeOYl916LTHnVbWwfeKgRoOivR1rqzXef6YS1AnrIX1777pZ9wcGW1aEezjzTZeGmCJfP3z0w7sw3fjZKNrqEtBk9vDGL4tmDKZqLkcBztjGFJpjQVSrVayv9AGw21FPXB9dk16mJIKaK_nQiEOSTDlGSYYzIMqII_-XOLW_h3FOQv7cm7ow</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Nobrega de Almeida, Jr, João</creator><creator>Del Negro, Gilda M B</creator><creator>Grenfell, Rafaella C</creator><creator>Vidal, Monica S M</creator><creator>Thomaz, Danilo Y</creator><creator>de Figueiredo, Dulce S Y</creator><creator>Bagagli, Eduardo</creator><creator>Juliano, Luiz</creator><creator>Benard, Gil</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope><scope>M7N</scope><scope>5PM</scope></search><sort><creationdate>20150401</creationdate><title>Matrix-assisted laser desorption ionization-time of flight mass spectrometry for differentiation of the dimorphic fungal species Paracoccidioides brasiliensis and Paracoccidioides lutzii</title><author>Nobrega de Almeida, Jr, João ; Del Negro, Gilda M B ; Grenfell, Rafaella C ; Vidal, Monica S M ; Thomaz, Danilo Y ; de Figueiredo, Dulce S Y ; Bagagli, Eduardo ; Juliano, Luiz ; Benard, Gil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-e2cf8fc5194e334f73107b4c3eb87ec5fe572ac1fa4e1168a3ea258a88cbd9013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>DNA, Fungal - genetics</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>HSP70 Heat-Shock Proteins - genetics</topic><topic>Mycology</topic><topic>Paracoccidioides</topic><topic>Paracoccidioides - classification</topic><topic>Paracoccidioides - genetics</topic><topic>Paracoccidioides brasiliensis</topic><topic>Polymerase Chain Reaction - methods</topic><topic>RNA, Fungal - genetics</topic><topic>Species Specificity</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nobrega de Almeida, Jr, João</creatorcontrib><creatorcontrib>Del Negro, Gilda M B</creatorcontrib><creatorcontrib>Grenfell, Rafaella C</creatorcontrib><creatorcontrib>Vidal, Monica S M</creatorcontrib><creatorcontrib>Thomaz, Danilo Y</creatorcontrib><creatorcontrib>de Figueiredo, Dulce S Y</creatorcontrib><creatorcontrib>Bagagli, Eduardo</creatorcontrib><creatorcontrib>Juliano, Luiz</creatorcontrib><creatorcontrib>Benard, Gil</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nobrega de Almeida, Jr, João</au><au>Del Negro, Gilda M B</au><au>Grenfell, Rafaella C</au><au>Vidal, Monica S M</au><au>Thomaz, Danilo Y</au><au>de Figueiredo, Dulce S Y</au><au>Bagagli, Eduardo</au><au>Juliano, Luiz</au><au>Benard, Gil</au><au>Warnock, D. W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Matrix-assisted laser desorption ionization-time of flight mass spectrometry for differentiation of the dimorphic fungal species Paracoccidioides brasiliensis and Paracoccidioides lutzii</atitle><jtitle>Journal of clinical microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>53</volume><issue>4</issue><spage>1383</spage><epage>1386</epage><pages>1383-1386</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><abstract>Isolates of Paracoccidioides brasiliensis and Paracoccidioides lutzii, previously characterized by molecular techniques, were identified for the first time by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). All isolates were correctly identified, with log score values of >2.0. 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| source | MEDLINE; American Society for Microbiology Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | DNA, Fungal - genetics DNA, Ribosomal Spacer - genetics HSP70 Heat-Shock Proteins - genetics Mycology Paracoccidioides Paracoccidioides - classification Paracoccidioides - genetics Paracoccidioides brasiliensis Polymerase Chain Reaction - methods RNA, Fungal - genetics Species Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title | Matrix-assisted laser desorption ionization-time of flight mass spectrometry for differentiation of the dimorphic fungal species Paracoccidioides brasiliensis and Paracoccidioides lutzii |
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