Matrix-assisted laser desorption ionization-time of flight mass spectrometry for differentiation of the dimorphic fungal species Paracoccidioides brasiliensis and Paracoccidioides lutzii

Isolates of Paracoccidioides brasiliensis and Paracoccidioides lutzii, previously characterized by molecular techniques, were identified for the first time by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). All isolates were correctly identified, with log...

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Veröffentlicht in:	Journal of clinical microbiology 2015-04, Vol.53 (4), p.1383-1386
Hauptverfasser:	Nobrega de Almeida, Jr, João, Del Negro, Gilda M B, Grenfell, Rafaella C, Vidal, Monica S M, Thomaz, Danilo Y, de Figueiredo, Dulce S Y, Bagagli, Eduardo, Juliano, Luiz, Benard, Gil
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA, Fungal - genetics DNA, Ribosomal Spacer - genetics HSP70 Heat-Shock Proteins - genetics Mycology Paracoccidioides Paracoccidioides - classification Paracoccidioides - genetics Paracoccidioides brasiliensis Polymerase Chain Reaction - methods RNA, Fungal - genetics Species Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Zusammenfassung:	Isolates of Paracoccidioides brasiliensis and Paracoccidioides lutzii, previously characterized by molecular techniques, were identified for the first time by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). All isolates were correctly identified, with log score values of >2.0. Thus, MALDI-TOF MS is a new tool for differentiating species of the genus Paracoccidioides.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.02847-14