Hematopoietic Cancer Cell Lines Can Support Replication of Sabin Poliovirus Type 1
Viral vaccines can be produced in adherent or in suspension cells. The objective of this work was to screen human suspension cell lines for the capacity to support viral replication. As the first step, it was investigated whether poliovirus can replicate in such cell lines. Sabin poliovirus type 1 w...
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description | Viral vaccines can be produced in adherent or in suspension cells. The objective of this work was to screen human suspension cell lines for the capacity to support viral replication. As the first step, it was investigated whether poliovirus can replicate in such cell lines. Sabin poliovirus type 1 was serially passaged on five human cell lines, HL60, K562, KG1, THP-1, and U937. Sabin type 1 was capable of efficiently replicating in three cell lines (K562, KG1, and U937), yielding high viral titers after replication. Expression of CD155, the poliovirus receptor, did not explain susceptibility to replication, since all cell lines expressed CD155. Furthermore, we showed that passaged virus replicated more efficiently than parental virus in KG1 cells, yielding higher virus titers in the supernatant early after infection. Infection of cell lines at an MOI of 0.01 resulted in high viral titers in the supernatant at day 4. Infection of K562 with passaged Sabin type 1 in a bioreactor system yielded high viral titers in the supernatant. Altogether, these data suggest that K562, KG1, and U937 cell lines are useful for propagation of poliovirus. |
doi_str_mv | 10.1155/2015/358462 |
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M.</creator><contributor>Tulkens, Paul M.</contributor><creatorcontrib>van der Pol, Leo A. ; de Gruijl, Tanja D. ; van Eikenhorst, Gerco ; van de Weerd, Gerard ; Oosterhoff, Dinja ; Bakker, Wilfried A. M. ; Tulkens, Paul M.</creatorcontrib><description>Viral vaccines can be produced in adherent or in suspension cells. The objective of this work was to screen human suspension cell lines for the capacity to support viral replication. As the first step, it was investigated whether poliovirus can replicate in such cell lines. Sabin poliovirus type 1 was serially passaged on five human cell lines, HL60, K562, KG1, THP-1, and U937. Sabin type 1 was capable of efficiently replicating in three cell lines (K562, KG1, and U937), yielding high viral titers after replication. Expression of CD155, the poliovirus receptor, did not explain susceptibility to replication, since all cell lines expressed CD155. Furthermore, we showed that passaged virus replicated more efficiently than parental virus in KG1 cells, yielding higher virus titers in the supernatant early after infection. Infection of cell lines at an MOI of 0.01 resulted in high viral titers in the supernatant at day 4. Infection of K562 with passaged Sabin type 1 in a bioreactor system yielded high viral titers in the supernatant. Altogether, these data suggest that K562, KG1, and U937 cell lines are useful for propagation of poliovirus.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2015/358462</identifier><identifier>PMID: 25815312</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Animals ; Cancer cells ; Cell Line, Tumor ; Cell lines ; Cercopithecus aethiops ; CHO Cells ; Coxsackie and Adenovirus Receptor-Like Membrane Protein - metabolism ; Cricetinae ; Cricetulus ; Genetic aspects ; Health aspects ; Hematologic Neoplasms - pathology ; Hematologic Neoplasms - virology ; Hepatitis ; Humans ; Infections ; Kinetics ; Poliovirus ; Poliovirus - physiology ; Receptors, Virus - metabolism ; Tetraspanin 28 - metabolism ; Vaccines ; Vero Cells ; Viral Load ; Virus Replication ; Viruses</subject><ispartof>BioMed research international, 2015-01, Vol.2015 (2015), p.1-11</ispartof><rights>Copyright © 2015 Dinja Oosterhoff et al.</rights><rights>COPYRIGHT 2015 John Wiley & Sons, Inc.</rights><rights>Copyright © 2015 Dinja Oosterhoff et al. Dinja Oosterhoff et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2015 Dinja Oosterhoff et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c594t-a29738d55c54c487219765e6c78f9f9c73bd5cfe5521d991a7947064b59baa7f3</citedby><cites>FETCH-LOGICAL-c594t-a29738d55c54c487219765e6c78f9f9c73bd5cfe5521d991a7947064b59baa7f3</cites><orcidid>0000-0001-8707-2266 ; 0000-0002-3589-8450</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359862/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359862/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25815312$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tulkens, Paul M.</contributor><creatorcontrib>van der Pol, Leo A.</creatorcontrib><creatorcontrib>de Gruijl, Tanja D.</creatorcontrib><creatorcontrib>van Eikenhorst, Gerco</creatorcontrib><creatorcontrib>van de Weerd, Gerard</creatorcontrib><creatorcontrib>Oosterhoff, Dinja</creatorcontrib><creatorcontrib>Bakker, Wilfried A. M.</creatorcontrib><title>Hematopoietic Cancer Cell Lines Can Support Replication of Sabin Poliovirus Type 1</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Viral vaccines can be produced in adherent or in suspension cells. The objective of this work was to screen human suspension cell lines for the capacity to support viral replication. As the first step, it was investigated whether poliovirus can replicate in such cell lines. Sabin poliovirus type 1 was serially passaged on five human cell lines, HL60, K562, KG1, THP-1, and U937. Sabin type 1 was capable of efficiently replicating in three cell lines (K562, KG1, and U937), yielding high viral titers after replication. Expression of CD155, the poliovirus receptor, did not explain susceptibility to replication, since all cell lines expressed CD155. Furthermore, we showed that passaged virus replicated more efficiently than parental virus in KG1 cells, yielding higher virus titers in the supernatant early after infection. Infection of cell lines at an MOI of 0.01 resulted in high viral titers in the supernatant at day 4. Infection of K562 with passaged Sabin type 1 in a bioreactor system yielded high viral titers in the supernatant. Altogether, these data suggest that K562, KG1, and U937 cell lines are useful for propagation of poliovirus.</description><subject>Animals</subject><subject>Cancer cells</subject><subject>Cell Line, Tumor</subject><subject>Cell lines</subject><subject>Cercopithecus aethiops</subject><subject>CHO Cells</subject><subject>Coxsackie and Adenovirus Receptor-Like Membrane Protein - metabolism</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Hematologic Neoplasms - pathology</subject><subject>Hematologic Neoplasms - virology</subject><subject>Hepatitis</subject><subject>Humans</subject><subject>Infections</subject><subject>Kinetics</subject><subject>Poliovirus</subject><subject>Poliovirus - physiology</subject><subject>Receptors, Virus - metabolism</subject><subject>Tetraspanin 28 - metabolism</subject><subject>Vaccines</subject><subject>Vero Cells</subject><subject>Viral Load</subject><subject>Virus Replication</subject><subject>Viruses</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNksFrFDEUxoMottSevEvAi1TWzpvkJZOLUBa1woLS1nPIZDJtymwyJjOV_vdm2LpWT80lIfnxvfe9L4S8huoDAOJpXQGeMmy4qJ-Rw5oBXwng8Hx_ZuyAHOd8W5XVgKiUeEkOamwAGdSH5OLcbc0Ux-jd5C1dm2Bdoms3DHTjg8vLDb2cxzGmiV64cfDWTD4GGnt6aVof6Pc4-Hjn05zp1f3oKLwiL3ozZHf8sB-RH58_Xa3PV5tvX76uzzYri4pPK1MryZoO0SK3vJE1KCnQCSubXvXKStZ2aHuHWEOnFBipuKwEb1G1xsieHZGPO91xbreusy5MyQx6TH5r0r2Oxut_X4K_0dfxTnOGqhF1EXj3IJDiz9nlSW99tsW6CS7OWYOQoqCI7AmokIw3DUBB3_6H3sY5hTKJhYIydQT5l7o2g9M-9LG0aBdRfcZLOqIoLWXf7yibYs7J9Xt3UOklf73kr3f5F_rN44Hs2T9pF-BkB9z40Jlf_mlq5YOUyuYRjMWBYr8BbJ6-CQ</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>van der Pol, Leo A.</creator><creator>de Gruijl, Tanja D.</creator><creator>van Eikenhorst, Gerco</creator><creator>van de Weerd, Gerard</creator><creator>Oosterhoff, Dinja</creator><creator>Bakker, Wilfried A. 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M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c594t-a29738d55c54c487219765e6c78f9f9c73bd5cfe5521d991a7947064b59baa7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Cancer cells</topic><topic>Cell Line, Tumor</topic><topic>Cell lines</topic><topic>Cercopithecus aethiops</topic><topic>CHO Cells</topic><topic>Coxsackie and Adenovirus Receptor-Like Membrane Protein - metabolism</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Hematologic Neoplasms - pathology</topic><topic>Hematologic Neoplasms - virology</topic><topic>Hepatitis</topic><topic>Humans</topic><topic>Infections</topic><topic>Kinetics</topic><topic>Poliovirus</topic><topic>Poliovirus - physiology</topic><topic>Receptors, Virus - metabolism</topic><topic>Tetraspanin 28 - metabolism</topic><topic>Vaccines</topic><topic>Vero Cells</topic><topic>Viral Load</topic><topic>Virus Replication</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van der Pol, Leo A.</creatorcontrib><creatorcontrib>de Gruijl, Tanja D.</creatorcontrib><creatorcontrib>van Eikenhorst, Gerco</creatorcontrib><creatorcontrib>van de Weerd, Gerard</creatorcontrib><creatorcontrib>Oosterhoff, Dinja</creatorcontrib><creatorcontrib>Bakker, Wilfried A. 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M.</au><au>Tulkens, Paul M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hematopoietic Cancer Cell Lines Can Support Replication of Sabin Poliovirus Type 1</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>2015</volume><issue>2015</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Viral vaccines can be produced in adherent or in suspension cells. The objective of this work was to screen human suspension cell lines for the capacity to support viral replication. As the first step, it was investigated whether poliovirus can replicate in such cell lines. Sabin poliovirus type 1 was serially passaged on five human cell lines, HL60, K562, KG1, THP-1, and U937. Sabin type 1 was capable of efficiently replicating in three cell lines (K562, KG1, and U937), yielding high viral titers after replication. Expression of CD155, the poliovirus receptor, did not explain susceptibility to replication, since all cell lines expressed CD155. Furthermore, we showed that passaged virus replicated more efficiently than parental virus in KG1 cells, yielding higher virus titers in the supernatant early after infection. Infection of cell lines at an MOI of 0.01 resulted in high viral titers in the supernatant at day 4. Infection of K562 with passaged Sabin type 1 in a bioreactor system yielded high viral titers in the supernatant. Altogether, these data suggest that K562, KG1, and U937 cell lines are useful for propagation of poliovirus.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>25815312</pmid><doi>10.1155/2015/358462</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-8707-2266</orcidid><orcidid>https://orcid.org/0000-0002-3589-8450</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Animals Cancer cells Cell Line, Tumor Cell lines Cercopithecus aethiops CHO Cells Coxsackie and Adenovirus Receptor-Like Membrane Protein - metabolism Cricetinae Cricetulus Genetic aspects Health aspects Hematologic Neoplasms - pathology Hematologic Neoplasms - virology Hepatitis Humans Infections Kinetics Poliovirus Poliovirus - physiology Receptors, Virus - metabolism Tetraspanin 28 - metabolism Vaccines Vero Cells Viral Load Virus Replication Viruses |
title | Hematopoietic Cancer Cell Lines Can Support Replication of Sabin Poliovirus Type 1 |
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