Hematopoietic Cancer Cell Lines Can Support Replication of Sabin Poliovirus Type 1

Viral vaccines can be produced in adherent or in suspension cells. The objective of this work was to screen human suspension cell lines for the capacity to support viral replication. As the first step, it was investigated whether poliovirus can replicate in such cell lines. Sabin poliovirus type 1 w...

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Veröffentlicht in:BioMed research international 2015-01, Vol.2015 (2015), p.1-11
Hauptverfasser: van der Pol, Leo A., de Gruijl, Tanja D., van Eikenhorst, Gerco, van de Weerd, Gerard, Oosterhoff, Dinja, Bakker, Wilfried A. M.
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container_end_page 11
container_issue 2015
container_start_page 1
container_title BioMed research international
container_volume 2015
creator van der Pol, Leo A.
de Gruijl, Tanja D.
van Eikenhorst, Gerco
van de Weerd, Gerard
Oosterhoff, Dinja
Bakker, Wilfried A. M.
description Viral vaccines can be produced in adherent or in suspension cells. The objective of this work was to screen human suspension cell lines for the capacity to support viral replication. As the first step, it was investigated whether poliovirus can replicate in such cell lines. Sabin poliovirus type 1 was serially passaged on five human cell lines, HL60, K562, KG1, THP-1, and U937. Sabin type 1 was capable of efficiently replicating in three cell lines (K562, KG1, and U937), yielding high viral titers after replication. Expression of CD155, the poliovirus receptor, did not explain susceptibility to replication, since all cell lines expressed CD155. Furthermore, we showed that passaged virus replicated more efficiently than parental virus in KG1 cells, yielding higher virus titers in the supernatant early after infection. Infection of cell lines at an MOI of 0.01 resulted in high viral titers in the supernatant at day 4. Infection of K562 with passaged Sabin type 1 in a bioreactor system yielded high viral titers in the supernatant. Altogether, these data suggest that K562, KG1, and U937 cell lines are useful for propagation of poliovirus.
doi_str_mv 10.1155/2015/358462
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Furthermore, we showed that passaged virus replicated more efficiently than parental virus in KG1 cells, yielding higher virus titers in the supernatant early after infection. Infection of cell lines at an MOI of 0.01 resulted in high viral titers in the supernatant at day 4. Infection of K562 with passaged Sabin type 1 in a bioreactor system yielded high viral titers in the supernatant. 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M.</creatorcontrib><title>Hematopoietic Cancer Cell Lines Can Support Replication of Sabin Poliovirus Type 1</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Viral vaccines can be produced in adherent or in suspension cells. The objective of this work was to screen human suspension cell lines for the capacity to support viral replication. As the first step, it was investigated whether poliovirus can replicate in such cell lines. Sabin poliovirus type 1 was serially passaged on five human cell lines, HL60, K562, KG1, THP-1, and U937. Sabin type 1 was capable of efficiently replicating in three cell lines (K562, KG1, and U937), yielding high viral titers after replication. Expression of CD155, the poliovirus receptor, did not explain susceptibility to replication, since all cell lines expressed CD155. Furthermore, we showed that passaged virus replicated more efficiently than parental virus in KG1 cells, yielding higher virus titers in the supernatant early after infection. Infection of cell lines at an MOI of 0.01 resulted in high viral titers in the supernatant at day 4. Infection of K562 with passaged Sabin type 1 in a bioreactor system yielded high viral titers in the supernatant. 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M.</au><au>Tulkens, Paul M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hematopoietic Cancer Cell Lines Can Support Replication of Sabin Poliovirus Type 1</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>2015</volume><issue>2015</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Viral vaccines can be produced in adherent or in suspension cells. The objective of this work was to screen human suspension cell lines for the capacity to support viral replication. As the first step, it was investigated whether poliovirus can replicate in such cell lines. Sabin poliovirus type 1 was serially passaged on five human cell lines, HL60, K562, KG1, THP-1, and U937. Sabin type 1 was capable of efficiently replicating in three cell lines (K562, KG1, and U937), yielding high viral titers after replication. Expression of CD155, the poliovirus receptor, did not explain susceptibility to replication, since all cell lines expressed CD155. Furthermore, we showed that passaged virus replicated more efficiently than parental virus in KG1 cells, yielding higher virus titers in the supernatant early after infection. Infection of cell lines at an MOI of 0.01 resulted in high viral titers in the supernatant at day 4. Infection of K562 with passaged Sabin type 1 in a bioreactor system yielded high viral titers in the supernatant. Altogether, these data suggest that K562, KG1, and U937 cell lines are useful for propagation of poliovirus.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>25815312</pmid><doi>10.1155/2015/358462</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-8707-2266</orcidid><orcidid>https://orcid.org/0000-0002-3589-8450</orcidid><oa>free_for_read</oa></addata></record>
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subjects Animals
Cancer cells
Cell Line, Tumor
Cell lines
Cercopithecus aethiops
CHO Cells
Coxsackie and Adenovirus Receptor-Like Membrane Protein - metabolism
Cricetinae
Cricetulus
Genetic aspects
Health aspects
Hematologic Neoplasms - pathology
Hematologic Neoplasms - virology
Hepatitis
Humans
Infections
Kinetics
Poliovirus
Poliovirus - physiology
Receptors, Virus - metabolism
Tetraspanin 28 - metabolism
Vaccines
Vero Cells
Viral Load
Virus Replication
Viruses
title Hematopoietic Cancer Cell Lines Can Support Replication of Sabin Poliovirus Type 1
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