Integration-free Methods for Generating Induced Pluripotent Stem Cells
Induced pluripotent stem (iPS) cells can be generated from mouse or human fibroblasts by exogenous expression of four factors, Oct4, Sox2, Klf4 and c-Myc, and hold great potential for transplantation therapies and regenerative medicine. However, use of retroviral vectors during iPS cell generation h...
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| Veröffentlicht in: | Genomics, proteomics & bioinformatics proteomics & bioinformatics, 2013-10, Vol.11 (5), p.284-287 |
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| creator | Zhou, Yi-ye Zeng, Fanyi |
| description | Induced pluripotent stem (iPS) cells can be generated from mouse or human fibroblasts by exogenous expression of four factors, Oct4, Sox2, Klf4 and c-Myc, and hold great potential for transplantation therapies and regenerative medicine. However, use of retroviral vectors during iPS cell generation has limited the technique’s clinical application due to the potential risks resulting from genome integration of transgenes, including insertional mutations and altered differentiation potentials of the target cells, which may lead to pathologies such as tumorigenesis. Here we review recent progress in generating safer transgene-free or integration-free iPS cells, including the use of non-integrating vectors, excision of vectors after integration, DNA-free delivery of factors and chemical induction of pluripotency. |
| doi_str_mv | 10.1016/j.gpb.2013.09.008 |
| format | Article |
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However, use of retroviral vectors during iPS cell generation has limited the technique’s clinical application due to the potential risks resulting from genome integration of transgenes, including insertional mutations and altered differentiation potentials of the target cells, which may lead to pathologies such as tumorigenesis. Here we review recent progress in generating safer transgene-free or integration-free iPS cells, including the use of non-integrating vectors, excision of vectors after integration, DNA-free delivery of factors and chemical induction of pluripotency.</description><identifier>ISSN: 1672-0229</identifier><identifier>EISSN: 2210-3244</identifier><identifier>DOI: 10.1016/j.gpb.2013.09.008</identifier><identifier>PMID: 24121200</identifier><language>eng</language><publisher>China: Elsevier Ltd</publisher><subject>Animals ; c-Myc ; carcinogenesis ; Cell Culture Techniques - methods ; Cell Differentiation - genetics ; Cell Separation - methods ; fibroblasts ; Genetic Vectors ; Humans ; Induced pluripotent stem (iPS) cells ; induced pluripotent stem cells ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - metabolism ; iPS ; Liposomes - chemistry ; Liposomes - metabolism ; medicine ; mice ; mutation ; retroviral vectors ; Review ; risk ; Transgene-free ; transgenes ; Vector ; 一体化 ; 人成纤维细胞 ; 化学诱导 ; 多能干细胞 ; 整合载体 ; 逆转录病毒载体</subject><ispartof>Genomics, proteomics & bioinformatics, 2013-10, Vol.11 (5), p.284-287</ispartof><rights>2013</rights><rights>Copyright © 2013. Production and hosting by Elsevier Ltd.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2013 Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China. Production and hosting by Elsevier B.V. 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However, use of retroviral vectors during iPS cell generation has limited the technique’s clinical application due to the potential risks resulting from genome integration of transgenes, including insertional mutations and altered differentiation potentials of the target cells, which may lead to pathologies such as tumorigenesis. 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| ispartof | Genomics, proteomics & bioinformatics, 2013-10, Vol.11 (5), p.284-287 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Access via Oxford University Press (Open Access Collection); Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals c-Myc carcinogenesis Cell Culture Techniques - methods Cell Differentiation - genetics Cell Separation - methods fibroblasts Genetic Vectors Humans Induced pluripotent stem (iPS) cells induced pluripotent stem cells Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism iPS Liposomes - chemistry Liposomes - metabolism medicine mice mutation retroviral vectors Review risk Transgene-free transgenes Vector 一体化 人成纤维细胞 化学诱导 多能干细胞 整合载体 逆转录病毒载体 |
| title | Integration-free Methods for Generating Induced Pluripotent Stem Cells |
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