Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells
Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using...
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| Veröffentlicht in: | Genomics, proteomics & bioinformatics proteomics & bioinformatics, 2013-10, Vol.11 (5), p.304-311 |
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| container_title | Genomics, proteomics & bioinformatics |
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| creator | Wang, Juan Gu, Qi Hao, Jie Bai, Donghui Liu, Lei Zhao, Xiaoyang Liu, Zhonghua Wang, Liu Zhou, Qi |
| description | Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs (UCB-iPSCs) have characteristics that are identical to pluripotent human embryonic stem cells (hESCs). This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases. |
| doi_str_mv | 10.1016/j.gpb.2013.08.002 |
| format | Article |
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Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs (UCB-iPSCs) have characteristics that are identical to pluripotent human embryonic stem cells (hESCs). This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.</description><identifier>ISSN: 1672-0229</identifier><identifier>EISSN: 2210-3244</identifier><identifier>DOI: 10.1016/j.gpb.2013.08.002</identifier><identifier>PMID: 24080286</identifier><language>eng</language><publisher>China: Elsevier Ltd</publisher><subject>Biomarkers - metabolism ; blood ; Cell Culture Techniques - methods ; Cell Differentiation ; Cell Separation - methods ; Dox-inducible lentiviral system ; embryonic stem cells ; Fetal Blood - cytology ; Fetal Blood - metabolism ; Germ Layers - cytology ; Germ Layers - metabolism ; Human iPSCs ; Human UCBMCs ; Humans ; induced pluripotent stem cells ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - metabolism ; Infant, Newborn ; iPS ; Lentivirus - genetics ; medicine ; neonates ; Original Research ; patients ; therapeutics ; umbilical cord ; 人类胚胎干细胞 ; 免疫学 ; 单个核细胞 ; 多能干细胞 ; 新生儿 ; 脐血 ; 诱导</subject><ispartof>Genomics, proteomics & bioinformatics, 2013-10, Vol.11 (5), p.304-311</ispartof><rights>2013</rights><rights>Copyright © 2013. Production and hosting by Elsevier Ltd.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2013 Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China. Production and hosting by Elsevier B.V. All rights reserved. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4919-482c99f59d572365b3f078bbcf9bc86b07197a9834731630c860a70ce2a9bfda3</citedby><cites>FETCH-LOGICAL-c4919-482c99f59d572365b3f078bbcf9bc86b07197a9834731630c860a70ce2a9bfda3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86775X/86775X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357789/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.gpb.2013.08.002$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,3550,27924,27925,45995,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24080286$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Juan</creatorcontrib><creatorcontrib>Gu, Qi</creatorcontrib><creatorcontrib>Hao, Jie</creatorcontrib><creatorcontrib>Bai, Donghui</creatorcontrib><creatorcontrib>Liu, Lei</creatorcontrib><creatorcontrib>Zhao, Xiaoyang</creatorcontrib><creatorcontrib>Liu, Zhonghua</creatorcontrib><creatorcontrib>Wang, Liu</creatorcontrib><creatorcontrib>Zhou, Qi</creatorcontrib><title>Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells</title><title>Genomics, proteomics & bioinformatics</title><addtitle>Genomics Proteomics ＆ Bioinformatics</addtitle><description>Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs (UCB-iPSCs) have characteristics that are identical to pluripotent human embryonic stem cells (hESCs). This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.</description><subject>Biomarkers - metabolism</subject><subject>blood</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation</subject><subject>Cell Separation - methods</subject><subject>Dox-inducible lentiviral system</subject><subject>embryonic stem cells</subject><subject>Fetal Blood - cytology</subject><subject>Fetal Blood - metabolism</subject><subject>Germ Layers - cytology</subject><subject>Germ Layers - metabolism</subject><subject>Human iPSCs</subject><subject>Human UCBMCs</subject><subject>Humans</subject><subject>induced pluripotent stem cells</subject><subject>Induced Pluripotent Stem Cells - cytology</subject><subject>Induced Pluripotent Stem Cells - metabolism</subject><subject>Infant, Newborn</subject><subject>iPS</subject><subject>Lentivirus - genetics</subject><subject>medicine</subject><subject>neonates</subject><subject>Original Research</subject><subject>patients</subject><subject>therapeutics</subject><subject>umbilical cord</subject><subject>人类胚胎干细胞</subject><subject>免疫学</subject><subject>单个核细胞</subject><subject>多能干细胞</subject><subject>新生儿</subject><subject>脐血</subject><subject>诱导</subject><issn>1672-0229</issn><issn>2210-3244</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV1v0zAUhiMEYmPwA7gBc8dNy_FH4kRISFCNddIQSKPXluPYqavE7uxk_fj1uLQbcMOVJfucx-85T5a9xjDFgIsPq2m7rqcEMJ1COQUgT7JzQjBMKGHsaXaOC04mQEh1lr2IcQXAcsbw8-yMMCiBlMV5Fq-000EO1jvkDbp2zah0g350Y7BrP2g3oNtB92imuy6ijR2WaG7bJbo0xiqrndohE3yP5mMvHVr0te2skh2a-dCgL533DfrmnXej6rQMR8zL7JmRXdSvTudFtvh6-XM2n9x8v7qefb6ZKFbhasJKoqrK5FWTc0KLvKYGeFnXylS1KosaOK64rErKOMUFhXQHkoPSRFa1aSS9yD4dueux7nWj0jBBdmIdbC_DTnhpxb8vzi5F6-8FoznnZZUA-AjYSGeka8XKj8GlyGK12zf1fr-Lm-12Wwt9cAA5wKHn_enT4O9GHQfR26jS2NJpP0aB82Qlp5zDH7wKPsagzWM0DOIgWKxEEiwOcAGlSIJTz5u_Z3rseDCaCt4eC4z0QrbBRrG4TYQUDhil9PDtx2OFTru_tzqI-NukbmzQahCNt_8N8O4Ueulde2fTVh4yMM4xoSynvwC-hcw1</recordid><startdate>201310</startdate><enddate>201310</enddate><creator>Wang, Juan</creator><creator>Gu, Qi</creator><creator>Hao, Jie</creator><creator>Bai, Donghui</creator><creator>Liu, Lei</creator><creator>Zhao, Xiaoyang</creator><creator>Liu, Zhonghua</creator><creator>Wang, Liu</creator><creator>Zhou, Qi</creator><general>Elsevier Ltd</general><general>College of Life Science, Northeast Agricultural University of China, Harbin 150030, China%State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China%State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China</general><general>State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China</general><general>Graduate School of Chinese Academy of Sciences, Beijing 100049, China%College of Life Science, Northeast Agricultural University of China, Harbin 150030, China</general><general>Elsevier</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>201310</creationdate><title>Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells</title><author>Wang, Juan ; 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Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs (UCB-iPSCs) have characteristics that are identical to pluripotent human embryonic stem cells (hESCs). This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.</abstract><cop>China</cop><pub>Elsevier Ltd</pub><pmid>24080286</pmid><doi>10.1016/j.gpb.2013.08.002</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-0229 |
| ispartof | Genomics, proteomics & bioinformatics, 2013-10, Vol.11 (5), p.304-311 |
| issn | 1672-0229 2210-3244 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4357789 |
| source | OUP_牛津大学出版社OA刊; MEDLINE; Elsevier ScienceDirect Journals Complete; DOAJ Directory of Open Access Journals; PubMed Central; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Biomarkers - metabolism blood Cell Culture Techniques - methods Cell Differentiation Cell Separation - methods Dox-inducible lentiviral system embryonic stem cells Fetal Blood - cytology Fetal Blood - metabolism Germ Layers - cytology Germ Layers - metabolism Human iPSCs Human UCBMCs Humans induced pluripotent stem cells Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Infant, Newborn iPS Lentivirus - genetics medicine neonates Original Research patients therapeutics umbilical cord 人类胚胎干细胞 免疫学 单个核细胞 多能干细胞 新生儿 脐血 诱导 |
| title | Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells |
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