Colostrum hexasaccharide, a novel Staphylococcus aureus quorum-sensing inhibitor
The discovery of quorum-sensing (QS) systems regulating antibiotic resistance and virulence factors (VFs) has afforded a novel opportunity to prevent bacterial pathogenicity. Dietary molecules have been demonstrated to attenuate QS circuits of bacteria. But, to our knowledge, no study exploring the...
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| Veröffentlicht in: | Antimicrobial agents and chemotherapy 2015-04, Vol.59 (4), p.2169-2178 |
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| creator | Srivastava, A Singh, B N Deepak, D Rawat, A K S Singh, B R |
| description | The discovery of quorum-sensing (QS) systems regulating antibiotic resistance and virulence factors (VFs) has afforded a novel opportunity to prevent bacterial pathogenicity. Dietary molecules have been demonstrated to attenuate QS circuits of bacteria. But, to our knowledge, no study exploring the potential of colostrum hexasaccharide (CHS) in regulating QS systems has been published. In this study, we analyzed CHS for inhibiting QS signaling in Staphylococcus aureus. We isolated and characterized CHS from mare colostrum by high-performance thin-layer chromatography (HPTLC), reverse-phase high-performance liquid chromatography evaporative light-scattering detection (RP-HPLC-ELSD), (1)H and (13)C nuclear magnetic resonance (NMR), and electrospray ionization mass spectrometry (ESI-MS). Antibiofilm activity of CHS against S. aureus and its possible interference with bacterial QS systems were determined. The inhibition and eradication potentials of the biofilms were studied by microscopic analyses and quantified by 96-well-microtiter-plate assays. Also, the ability of CHS to interfere in bacterial QS by degrading acyl-homoserine lactones (AHLs), one of the most studied signal molecules for Gram-negative bacteria, was evaluated. The results revealed that CHS exhibited promising inhibitory activities against QS-regulated secretion of VFs, including spreading ability, hemolysis, protease, and lipase activities, when applied at a rate of 5 mg/ml. The results of biofilm experiments indicated that CHS is a strong inhibitor of biofilm formation and also has the ability to eradicate it. The potential of CHS to interfere with bacterial QS systems was also examined by degradation of AHLs. Furthermore, it was documented that CHS decreased antibiotic resistance in S. aureus. The results thus give a lead that mare colostrum can be a promising source for isolating a next-generation antibacterial. |
| doi_str_mv | 10.1128/AAC.03722-14 |
| format | Article |
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Dietary molecules have been demonstrated to attenuate QS circuits of bacteria. But, to our knowledge, no study exploring the potential of colostrum hexasaccharide (CHS) in regulating QS systems has been published. In this study, we analyzed CHS for inhibiting QS signaling in Staphylococcus aureus. We isolated and characterized CHS from mare colostrum by high-performance thin-layer chromatography (HPTLC), reverse-phase high-performance liquid chromatography evaporative light-scattering detection (RP-HPLC-ELSD), (1)H and (13)C nuclear magnetic resonance (NMR), and electrospray ionization mass spectrometry (ESI-MS). Antibiofilm activity of CHS against S. aureus and its possible interference with bacterial QS systems were determined. The inhibition and eradication potentials of the biofilms were studied by microscopic analyses and quantified by 96-well-microtiter-plate assays. Also, the ability of CHS to interfere in bacterial QS by degrading acyl-homoserine lactones (AHLs), one of the most studied signal molecules for Gram-negative bacteria, was evaluated. The results revealed that CHS exhibited promising inhibitory activities against QS-regulated secretion of VFs, including spreading ability, hemolysis, protease, and lipase activities, when applied at a rate of 5 mg/ml. The results of biofilm experiments indicated that CHS is a strong inhibitor of biofilm formation and also has the ability to eradicate it. The potential of CHS to interfere with bacterial QS systems was also examined by degradation of AHLs. Furthermore, it was documented that CHS decreased antibiotic resistance in S. aureus. The results thus give a lead that mare colostrum can be a promising source for isolating a next-generation antibacterial.</description><identifier>ISSN: 0066-4804</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AAC.03722-14</identifier><identifier>PMID: 25645850</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Acyl-Butyrolactones - metabolism ; Animals ; Anti-Bacterial Agents ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Biofilms - drug effects ; Carbohydrate Sequence ; Colostrum ; Colostrum - chemistry ; Experimental Therapeutics ; Female ; Hemolysis ; Horses ; Humans ; Indoles - antagonists & inhibitors ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Oligosaccharides ; Oligosaccharides - chemistry ; Oligosaccharides - pharmacology ; Pregnancy ; Quorum Sensing ; Quorum Sensing - drug effects ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Virulence Factors - metabolism</subject><ispartof>Antimicrobial agents and chemotherapy, 2015-04, Vol.59 (4), p.2169-2178</ispartof><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</rights><rights>Copyright © 2015, American Society for Microbiology. 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Dietary molecules have been demonstrated to attenuate QS circuits of bacteria. But, to our knowledge, no study exploring the potential of colostrum hexasaccharide (CHS) in regulating QS systems has been published. In this study, we analyzed CHS for inhibiting QS signaling in Staphylococcus aureus. We isolated and characterized CHS from mare colostrum by high-performance thin-layer chromatography (HPTLC), reverse-phase high-performance liquid chromatography evaporative light-scattering detection (RP-HPLC-ELSD), (1)H and (13)C nuclear magnetic resonance (NMR), and electrospray ionization mass spectrometry (ESI-MS). Antibiofilm activity of CHS against S. aureus and its possible interference with bacterial QS systems were determined. The inhibition and eradication potentials of the biofilms were studied by microscopic analyses and quantified by 96-well-microtiter-plate assays. Also, the ability of CHS to interfere in bacterial QS by degrading acyl-homoserine lactones (AHLs), one of the most studied signal molecules for Gram-negative bacteria, was evaluated. The results revealed that CHS exhibited promising inhibitory activities against QS-regulated secretion of VFs, including spreading ability, hemolysis, protease, and lipase activities, when applied at a rate of 5 mg/ml. The results of biofilm experiments indicated that CHS is a strong inhibitor of biofilm formation and also has the ability to eradicate it. The potential of CHS to interfere with bacterial QS systems was also examined by degradation of AHLs. Furthermore, it was documented that CHS decreased antibiotic resistance in S. aureus. The results thus give a lead that mare colostrum can be a promising source for isolating a next-generation antibacterial.</description><subject>Acyl-Butyrolactones - metabolism</subject><subject>Animals</subject><subject>Anti-Bacterial Agents</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Biofilms - drug effects</subject><subject>Carbohydrate Sequence</subject><subject>Colostrum</subject><subject>Colostrum - chemistry</subject><subject>Experimental Therapeutics</subject><subject>Female</subject><subject>Hemolysis</subject><subject>Horses</subject><subject>Humans</subject><subject>Indoles - antagonists & inhibitors</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Sequence Data</subject><subject>Oligosaccharides</subject><subject>Oligosaccharides - chemistry</subject><subject>Oligosaccharides - pharmacology</subject><subject>Pregnancy</subject><subject>Quorum Sensing</subject><subject>Quorum Sensing - drug effects</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Virulence Factors - metabolism</subject><issn>0066-4804</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1r3DAQxUVpaLbb3noOPrYQJ_rwyNIlsCxpEwgk0NyFLI9jBdvaSPaS_Pf1ZtPQHgI5DcP85jFvHiHfGD1hjKvT1Wp9QkXJec6KD2TBqFa5BC0_kgWlUuaFosUh-ZzSPZ170PQTOeQgC1BAF-RmHbqQxjj1WYuPNlnnWht9jceZzYawxS77PdpN-9QFF5ybUmaniHN5mMK8lCcckh_uMj-0vvJjiF_IQWO7hF9f6pLc_jy_XV_kV9e_Lterq9yCKsdcC0cdurKSTc2aipWVAECKCqDktKhpY4GjdqrRNVcKSweCgeQVFBrKQizJ2V52M1U91g6HMdrObKLvbXwywXrz_2TwrbkLW1MIkIrpWeD7i0AMDxOm0fQ-Oew6O2CYkmGylALmR4l3oFJIkFTTGT3eoy6GlCI2rxcxanZxmTku8xyXYTsXP_a4TT0392GKw_yzt9ijfx2_Cv_NUvwBBQKd8w</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Srivastava, A</creator><creator>Singh, B N</creator><creator>Deepak, D</creator><creator>Rawat, A K S</creator><creator>Singh, B R</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20150401</creationdate><title>Colostrum hexasaccharide, a novel Staphylococcus aureus quorum-sensing inhibitor</title><author>Srivastava, A ; Singh, B N ; Deepak, D ; Rawat, A K S ; Singh, B R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a587t-93c0cec7b6fd1fb17b355e0e8557204d0fa52e9c8f9d288e7c531562b5495743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acyl-Butyrolactones - metabolism</topic><topic>Animals</topic><topic>Anti-Bacterial Agents</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Biofilms - drug effects</topic><topic>Carbohydrate Sequence</topic><topic>Colostrum</topic><topic>Colostrum - chemistry</topic><topic>Experimental Therapeutics</topic><topic>Female</topic><topic>Hemolysis</topic><topic>Horses</topic><topic>Humans</topic><topic>Indoles - antagonists & inhibitors</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Sequence Data</topic><topic>Oligosaccharides</topic><topic>Oligosaccharides - chemistry</topic><topic>Oligosaccharides - pharmacology</topic><topic>Pregnancy</topic><topic>Quorum Sensing</topic><topic>Quorum Sensing - drug effects</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Virulence Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Srivastava, A</creatorcontrib><creatorcontrib>Singh, B N</creatorcontrib><creatorcontrib>Deepak, D</creatorcontrib><creatorcontrib>Rawat, A K S</creatorcontrib><creatorcontrib>Singh, B R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Antimicrobial agents and chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Srivastava, A</au><au>Singh, B N</au><au>Deepak, D</au><au>Rawat, A K S</au><au>Singh, B R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Colostrum hexasaccharide, a novel Staphylococcus aureus quorum-sensing inhibitor</atitle><jtitle>Antimicrobial agents and chemotherapy</jtitle><stitle>Antimicrob Agents Chemother</stitle><addtitle>Antimicrob Agents Chemother</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>59</volume><issue>4</issue><spage>2169</spage><epage>2178</epage><pages>2169-2178</pages><issn>0066-4804</issn><eissn>1098-6596</eissn><abstract>The discovery of quorum-sensing (QS) systems regulating antibiotic resistance and virulence factors (VFs) has afforded a novel opportunity to prevent bacterial pathogenicity. Dietary molecules have been demonstrated to attenuate QS circuits of bacteria. But, to our knowledge, no study exploring the potential of colostrum hexasaccharide (CHS) in regulating QS systems has been published. In this study, we analyzed CHS for inhibiting QS signaling in Staphylococcus aureus. We isolated and characterized CHS from mare colostrum by high-performance thin-layer chromatography (HPTLC), reverse-phase high-performance liquid chromatography evaporative light-scattering detection (RP-HPLC-ELSD), (1)H and (13)C nuclear magnetic resonance (NMR), and electrospray ionization mass spectrometry (ESI-MS). Antibiofilm activity of CHS against S. aureus and its possible interference with bacterial QS systems were determined. The inhibition and eradication potentials of the biofilms were studied by microscopic analyses and quantified by 96-well-microtiter-plate assays. Also, the ability of CHS to interfere in bacterial QS by degrading acyl-homoserine lactones (AHLs), one of the most studied signal molecules for Gram-negative bacteria, was evaluated. The results revealed that CHS exhibited promising inhibitory activities against QS-regulated secretion of VFs, including spreading ability, hemolysis, protease, and lipase activities, when applied at a rate of 5 mg/ml. The results of biofilm experiments indicated that CHS is a strong inhibitor of biofilm formation and also has the ability to eradicate it. The potential of CHS to interfere with bacterial QS systems was also examined by degradation of AHLs. Furthermore, it was documented that CHS decreased antibiotic resistance in S. aureus. The results thus give a lead that mare colostrum can be a promising source for isolating a next-generation antibacterial.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>25645850</pmid><doi>10.1128/AAC.03722-14</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acyl-Butyrolactones - metabolism Animals Anti-Bacterial Agents Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Biofilms - drug effects Carbohydrate Sequence Colostrum Colostrum - chemistry Experimental Therapeutics Female Hemolysis Horses Humans Indoles - antagonists & inhibitors Microbial Sensitivity Tests Molecular Sequence Data Oligosaccharides Oligosaccharides - chemistry Oligosaccharides - pharmacology Pregnancy Quorum Sensing Quorum Sensing - drug effects Staphylococcus aureus Staphylococcus aureus - drug effects Virulence Factors - metabolism |
| title | Colostrum hexasaccharide, a novel Staphylococcus aureus quorum-sensing inhibitor |
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