Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives
The aim of the study was to obtain transformed roots of Rhaponticum carthamoides and evaluate their phytochemical profile. Hairy roots were induced from leaf explants by the transformation of Agrobacterium rhizogenes strains A4 and ATCC 15834. The best response (43%) was achieved by infection with A...
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| creator | Kiss, Anna K. Olszewska, Monika A. Kicel, Agnieszka Skała, Ewa Wysokińska, Halina |
| description | The aim of the study was to obtain transformed roots of Rhaponticum carthamoides and evaluate their phytochemical profile. Hairy roots were induced from leaf explants by the transformation of Agrobacterium rhizogenes strains A4 and ATCC 15834. The best response (43%) was achieved by infection with A4 strain. The effects of different liquid media (WPM, B5, SH) with full and half-strength concentrations of macro- and micronutrients on biomass accumulation of the best grown hairy root line (RC3) at two different lighting conditions (light or dark) were investigated. The highest biomass (93 g L−1 of the fresh weight after 35 days) was obtained in WPM medium under periodic light. UPLC-PDA-ESI-MS3 and HPLC-PDA analyses of 80% aqueous methanol extracts from the obtained hairy roots revealed the presence of eleven caffeoylquinic acids and their derivatives and five flavonoid glycosides. The production of caffeoylquinic acids and their derivatives was elevated in hairy roots grown in the light. Only light-grown hairy roots demonstrated the capability for the biosynthesis of such flavonoid glycosides as quercetagetin, quercetin, luteolin, and patuletin hexosides. Chlorogenic acid, 3,5-di-O-caffeoylquinic acid and a tentatively identified tricaffeoylquinic acid derivative were detected as the major compounds present in the transformed roots. |
| doi_str_mv | 10.1155/2015/181098 |
| format | Article |
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Iljin for the Production of Biomass and Caffeic Acid Derivatives</title><source>MEDLINE</source><source>PubMed Central Open Access</source><source>Wiley-Blackwell Open Access Titles</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Kiss, Anna K. ; Olszewska, Monika A. ; Kicel, Agnieszka ; Skała, Ewa ; Wysokińska, Halina</creator><contributor>Reis, Alberto</contributor><creatorcontrib>Kiss, Anna K. ; Olszewska, Monika A. ; Kicel, Agnieszka ; Skała, Ewa ; Wysokińska, Halina ; Reis, Alberto</creatorcontrib><description>The aim of the study was to obtain transformed roots of Rhaponticum carthamoides and evaluate their phytochemical profile. Hairy roots were induced from leaf explants by the transformation of Agrobacterium rhizogenes strains A4 and ATCC 15834. The best response (43%) was achieved by infection with A4 strain. The effects of different liquid media (WPM, B5, SH) with full and half-strength concentrations of macro- and micronutrients on biomass accumulation of the best grown hairy root line (RC3) at two different lighting conditions (light or dark) were investigated. The highest biomass (93 g L−1 of the fresh weight after 35 days) was obtained in WPM medium under periodic light. UPLC-PDA-ESI-MS3 and HPLC-PDA analyses of 80% aqueous methanol extracts from the obtained hairy roots revealed the presence of eleven caffeoylquinic acids and their derivatives and five flavonoid glycosides. The production of caffeoylquinic acids and their derivatives was elevated in hairy roots grown in the light. Only light-grown hairy roots demonstrated the capability for the biosynthesis of such flavonoid glycosides as quercetagetin, quercetin, luteolin, and patuletin hexosides. Chlorogenic acid, 3,5-di-O-caffeoylquinic acid and a tentatively identified tricaffeoylquinic acid derivative were detected as the major compounds present in the transformed roots.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2015/181098</identifier><identifier>PMID: 25811023</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Acids ; Agrobacterium ; Agrobacterium rhizogenes ; Biomass ; Caffeic Acids - metabolism ; Caffeoylquinic acid ; Chromatography, High Pressure Liquid ; Colleges & universities ; Compositae ; Culture Media ; Derivatives ; DNA, Plant - isolation & purification ; Ecdysterone - metabolism ; Flavonoids ; Flowers & plants ; Glycosides ; Herbal medicine ; Infections ; Leuzea - growth & development ; Medical research ; Metabolites ; Methyl alcohol ; Plant Roots - growth & development ; Plant sciences ; Polymerase Chain Reaction ; Polyphenols - metabolism ; Production processes ; Roots ; Spectrometry, Mass, Electrospray Ionization ; Strain ; Tissue Culture Techniques - methods</subject><ispartof>BioMed research international, 2015-01, Vol.2015 (2015), p.1-11</ispartof><rights>Copyright © 2015 Ewa Skała et al.</rights><rights>COPYRIGHT 2015 John Wiley & Sons, Inc.</rights><rights>Copyright © 2015 Ewa Skala et al. Ewa Skala et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2015 Ewa Skała et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-deb081a7509c7562380486b6a508269f4386d5e217796386033e8b41877e373</citedby><cites>FETCH-LOGICAL-c524t-deb081a7509c7562380486b6a508269f4386d5e217796386033e8b41877e373</cites><orcidid>0000-0002-5182-6991</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354965/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354965/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25811023$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Reis, Alberto</contributor><creatorcontrib>Kiss, Anna K.</creatorcontrib><creatorcontrib>Olszewska, Monika A.</creatorcontrib><creatorcontrib>Kicel, Agnieszka</creatorcontrib><creatorcontrib>Skała, Ewa</creatorcontrib><creatorcontrib>Wysokińska, Halina</creatorcontrib><title>Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>The aim of the study was to obtain transformed roots of Rhaponticum carthamoides and evaluate their phytochemical profile. Hairy roots were induced from leaf explants by the transformation of Agrobacterium rhizogenes strains A4 and ATCC 15834. The best response (43%) was achieved by infection with A4 strain. The effects of different liquid media (WPM, B5, SH) with full and half-strength concentrations of macro- and micronutrients on biomass accumulation of the best grown hairy root line (RC3) at two different lighting conditions (light or dark) were investigated. The highest biomass (93 g L−1 of the fresh weight after 35 days) was obtained in WPM medium under periodic light. UPLC-PDA-ESI-MS3 and HPLC-PDA analyses of 80% aqueous methanol extracts from the obtained hairy roots revealed the presence of eleven caffeoylquinic acids and their derivatives and five flavonoid glycosides. The production of caffeoylquinic acids and their derivatives was elevated in hairy roots grown in the light. Only light-grown hairy roots demonstrated the capability for the biosynthesis of such flavonoid glycosides as quercetagetin, quercetin, luteolin, and patuletin hexosides. Chlorogenic acid, 3,5-di-O-caffeoylquinic acid and a tentatively identified tricaffeoylquinic acid derivative were detected as the major compounds present in the transformed roots.</description><subject>Acids</subject><subject>Agrobacterium</subject><subject>Agrobacterium rhizogenes</subject><subject>Biomass</subject><subject>Caffeic Acids - metabolism</subject><subject>Caffeoylquinic acid</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Colleges & universities</subject><subject>Compositae</subject><subject>Culture Media</subject><subject>Derivatives</subject><subject>DNA, Plant - isolation & purification</subject><subject>Ecdysterone - metabolism</subject><subject>Flavonoids</subject><subject>Flowers & plants</subject><subject>Glycosides</subject><subject>Herbal medicine</subject><subject>Infections</subject><subject>Leuzea - growth & development</subject><subject>Medical research</subject><subject>Metabolites</subject><subject>Methyl alcohol</subject><subject>Plant Roots - growth & development</subject><subject>Plant sciences</subject><subject>Polymerase Chain Reaction</subject><subject>Polyphenols - metabolism</subject><subject>Production processes</subject><subject>Roots</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Strain</subject><subject>Tissue Culture Techniques - methods</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkl9rFDEUxQdRbGn75LsEfKnKtvmfzIuwrrUtFJQq-BiymUwnS2ayJpmVfgU_tRm2LqtPm5dccn853Hs4VfUKwQuEGLvEELFLJBGs5bPqGBNEZxxR9HxXE3JUnaW0guVIxGHNX1ZHmEmEICbH1e-rlPXSu9T1dsggtOBGu_gI7kPIYDH6PEabpuf7Tq_DkJ0Ze2B0zJ3ug2tK7_yH8765eAtu_coNoA0R5M6CrzE0o8kuDNPvjy70OiWghwYsdNtaZ8DcuAZ8stFtdHYbm06rF632yZ493SfVt89X3xc3s7sv17eL-d3MMEzzrLHLsogWDNZGMI6JhFTyJdcMSszrlhLJG2YxEqLmpYaEWLmkSAphiSAn1Yet6npc9rYxZeuovVpH1-v4qIJ26t_O4Dr1EDaKEkZrzorA-ZNADD9Hm7LqXTLWez3YMCaFuGSCS0HxAajghJUx6wNQLgglnE2qb_5DV2GMQ7FsohBBmIg96kF7q9zQhrKNmUTVnLIpCRRPbrzfUiaGlKJtd0YgqKaAqSlgahuwQr_e927H_o1TAd5tgc4Njf7lDlOzBbGt3oMZZRiRP_ls3m4</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Kiss, Anna K.</creator><creator>Olszewska, Monika A.</creator><creator>Kicel, Agnieszka</creator><creator>Skała, Ewa</creator><creator>Wysokińska, Halina</creator><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7TB</scope><scope>7U5</scope><scope>F28</scope><scope>L7M</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5182-6991</orcidid></search><sort><creationdate>20150101</creationdate><title>Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) 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Iljin for the Production of Biomass and Caffeic Acid Derivatives</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>2015</volume><issue>2015</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>The aim of the study was to obtain transformed roots of Rhaponticum carthamoides and evaluate their phytochemical profile. Hairy roots were induced from leaf explants by the transformation of Agrobacterium rhizogenes strains A4 and ATCC 15834. The best response (43%) was achieved by infection with A4 strain. The effects of different liquid media (WPM, B5, SH) with full and half-strength concentrations of macro- and micronutrients on biomass accumulation of the best grown hairy root line (RC3) at two different lighting conditions (light or dark) were investigated. The highest biomass (93 g L−1 of the fresh weight after 35 days) was obtained in WPM medium under periodic light. UPLC-PDA-ESI-MS3 and HPLC-PDA analyses of 80% aqueous methanol extracts from the obtained hairy roots revealed the presence of eleven caffeoylquinic acids and their derivatives and five flavonoid glycosides. The production of caffeoylquinic acids and their derivatives was elevated in hairy roots grown in the light. Only light-grown hairy roots demonstrated the capability for the biosynthesis of such flavonoid glycosides as quercetagetin, quercetin, luteolin, and patuletin hexosides. Chlorogenic acid, 3,5-di-O-caffeoylquinic acid and a tentatively identified tricaffeoylquinic acid derivative were detected as the major compounds present in the transformed roots.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>25811023</pmid><doi>10.1155/2015/181098</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-5182-6991</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Agrobacterium Agrobacterium rhizogenes Biomass Caffeic Acids - metabolism Caffeoylquinic acid Chromatography, High Pressure Liquid Colleges & universities Compositae Culture Media Derivatives DNA, Plant - isolation & purification Ecdysterone - metabolism Flavonoids Flowers & plants Glycosides Herbal medicine Infections Leuzea - growth & development Medical research Metabolites Methyl alcohol Plant Roots - growth & development Plant sciences Polymerase Chain Reaction Polyphenols - metabolism Production processes Roots Spectrometry, Mass, Electrospray Ionization Strain Tissue Culture Techniques - methods |
| title | Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives |
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