Human amniotic epithelial cells express specific markers of nerve cells and migrate along the nerve fibers in the corpus callosum
Human amniotic epithelial cells were isolated from a piece of fresh amnion. Using immunocytochemical methods, we investigated the expression of neuronal phenotypes （microtubule-associated protein-2, glial fibrillary acidic protein and nestin） in human amniotic epithelial cells. The conditioned mediu...
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| Veröffentlicht in: | Neural regeneration research 2012-01, Vol.7 (1), p.41-45 |
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| creator | Wu, Zhiyuan Hui, Guozhen Lu, Yi Liu, Tianjin Huang, Qin Guo, Lihe |
| description | Human amniotic epithelial cells were isolated from a piece of fresh amnion. Using immunocytochemical methods, we investigated the expression of neuronal phenotypes （microtubule-associated protein-2, glial fibrillary acidic protein and nestin） in human amniotic epithelial cells. The conditioned medium of human amniotic epithelial cells promoted the growth and proliferation of rat glial cells cultured in vitro, and this effect was dose-dependent. Human amniotic epithelial cells were further transplanted into the corpus striatum of healthy adult rats and the grafted cells could integrate with the host and migrate 1 2 mm along the nerve fibers in corpus callosum. Our experimental findings indicate that human amniotic epithelial cells may be a new kind of seed cells for use in neurograft. |
| doi_str_mv | 10.3969/j.issn.1673-5374.2012.01.007 |
| format | Article |
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All Rights Reserved.</rights><rights>Copyright: © Neural Regeneration Research 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-a8fe22d9dc019e7c60a962419d6b56182cc77bebc2af9c1da7dce609bfad18883</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/88507X/88507X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354114/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354114/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25806057$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Zhiyuan</creatorcontrib><creatorcontrib>Hui, Guozhen</creatorcontrib><creatorcontrib>Lu, Yi</creatorcontrib><creatorcontrib>Liu, Tianjin</creatorcontrib><creatorcontrib>Huang, Qin</creatorcontrib><creatorcontrib>Guo, Lihe</creatorcontrib><title>Human amniotic epithelial cells express specific markers of nerve cells and migrate along the nerve fibers in the corpus callosum</title><title>Neural regeneration research</title><addtitle>Neural Regeneration Research</addtitle><description>Human amniotic epithelial cells were isolated from a piece of fresh amnion. Using immunocytochemical methods, we investigated the expression of neuronal phenotypes （microtubule-associated protein-2, glial fibrillary acidic protein and nestin） in human amniotic epithelial cells. The conditioned medium of human amniotic epithelial cells promoted the growth and proliferation of rat glial cells cultured in vitro, and this effect was dose-dependent. Human amniotic epithelial cells were further transplanted into the corpus striatum of healthy adult rats and the grafted cells could integrate with the host and migrate 1 2 mm along the nerve fibers in corpus callosum. Our experimental findings indicate that human amniotic epithelial cells may be a new kind of seed cells for use in neurograft.</description><subject>Animals</subject><subject>Experiments</subject><subject>Maternal & child health</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Nutrition</subject><subject>Placenta</subject><subject>Research and Report: Stem Cells and Neuroregenration</subject><subject>Stem cells</subject><subject>上皮细胞</subject><subject>免疫组织化学方法</subject><subject>标志物</subject><subject>神经纤维</subject><subject>细胞表达</subject><subject>羊膜</subject><subject>胼胝体</subject><subject>迁移</subject><issn>1673-5374</issn><issn>1876-7958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkU1v1DAQhiMEoqXwF5ARPSChBNtJ_HFBqiqgSJW4wNlynEnWIbFTOyltb_3neLu7FfTkkeeZd96ZybJTgotSMvlpKGyMriCMl3ld8qqgmNACkwJj_iw7JoKznMtaPE_xgTnKXsU4YFwLScuX2RGtBWa45sfZ_cU6aYf05KxfrEEw22UDo9UjMjCOEcHNHCBGFGcwtkvEpMNvCBH5DjkI17DntGvRZPugF0B69K5HSWdPdLbZVlj38Gd8mNeIjB5HH9fpdfai02OEN_v3JPv19cvP84v88se37-dnl7mpSL3kWnRAaStbg4kEbhjWktGKyJY1NSOCGsN5A42hupOGtJq3BhiWTadbIoQoT7LPO915bSZISbcEPao52DTRrfLaqv8zzm5U769VVdYVIVUS-LgT-KNdp12vBr8Glyyruz4Od_F2ULC9BSYYb9t92LcL_mqFuKjJxu2qtAO_RkUYY1JwKmhC3z9BH5VpKajAVGKZqLf_-n80frhlAt7tALNJ67-yyeGBqZKhEmNS_gU8yrLZ</recordid><startdate>20120105</startdate><enddate>20120105</enddate><creator>Wu, Zhiyuan</creator><creator>Hui, Guozhen</creator><creator>Lu, Yi</creator><creator>Liu, Tianjin</creator><creator>Huang, Qin</creator><creator>Guo, Lihe</creator><general>Medknow Publications & Media Pvt. 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Using immunocytochemical methods, we investigated the expression of neuronal phenotypes （microtubule-associated protein-2, glial fibrillary acidic protein and nestin） in human amniotic epithelial cells. The conditioned medium of human amniotic epithelial cells promoted the growth and proliferation of rat glial cells cultured in vitro, and this effect was dose-dependent. Human amniotic epithelial cells were further transplanted into the corpus striatum of healthy adult rats and the grafted cells could integrate with the host and migrate 1 2 mm along the nerve fibers in corpus callosum. Our experimental findings indicate that human amniotic epithelial cells may be a new kind of seed cells for use in neurograft.</abstract><cop>India</cop><pub>Medknow Publications & Media Pvt. Ltd</pub><pmid>25806057</pmid><doi>10.3969/j.issn.1673-5374.2012.01.007</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Neural regeneration research, 2012-01, Vol.7 (1), p.41-45 |
| issn | 1673-5374 1876-7958 |
| language | eng |
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| source | Medknow Open Access Medical Journals; Journals@Ovid Complete; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Experiments Maternal & child health Microscopy Morphology Nutrition Placenta Research and Report: Stem Cells and Neuroregenration Stem cells 上皮细胞 免疫组织化学方法 标志物 神经纤维 细胞表达 羊膜 胼胝体 迁移 |
| title | Human amniotic epithelial cells express specific markers of nerve cells and migrate along the nerve fibers in the corpus callosum |
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