Protective effect of Lai Fu Cheng Qi decoction on severe acute pancreatitis-induced myocardial injury in a rat model
The aim of the present study was to evaluate the effects of Lai Fu Cheng Qi decoction on myocardial injury in rats with severe acute pancreatitis (SAP). In total, 30 rats were randomly divided into sham, SAP and decoction treatment groups. SAP was induced by a retrograde pancreatic duct injection of...
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| Veröffentlicht in: | Experimental and therapeutic medicine 2015-04, Vol.9 (4), p.1133-1140 |
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| creator | LI, NAN TIAN, YING WANG, CHUNLI ZHANG, PENG YOU, SHENGYI |
| description | The aim of the present study was to evaluate the effects of Lai Fu Cheng Qi decoction on myocardial injury in rats with severe acute pancreatitis (SAP). In total, 30 rats were randomly divided into sham, SAP and decoction treatment groups. SAP was induced by a retrograde pancreatic duct injection of 5% sodium taurocholate in the SAP and decoction treatment groups. Rats in decoction treatment group also received intragastric administration of Lai Fu Cheng Qi decoction. The serum levels of creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) were detected using an automatic biochemical analyzer. In addition, myocardial Na+-K+-ATPase activity was analyzed using a spectrophotometric method and the mitochondrial membrane potential was measured by flow cytometry. Myocardial apoptosis was assessed using a TUNEL assay and pathological changes to the heart and pancreas were detected by hematoxylin and eosin staining. Compared with the rats in the sham group, rats in the SAP and decoction treatment groups exhibited significantly higher levels of serum CK-MB and LDH, apoptosis index and pathological scores, and had significantly lower levels of Na+-K+-ATPase activity and mitochondrial membrane potential. However, when compared with the SAP group, the serum levels of CK-MB and LDH, the pathological scores of the pancreas and heart, and the myocardial cell apoptosis index in the decoction treatment group were significantly lower. Furthermore, the Na+-K+-ATPase activity and mitochondrial membrane potential were significantly increased in the decoction treatment group when compared with the SAP group. Therefore, Lai Fu Cheng Qi decoction was shown to exert a protective effect on myocardial injury induced by SAP in rats. |
| doi_str_mv | 10.3892/etm.2015.2250 |
| format | Article |
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In total, 30 rats were randomly divided into sham, SAP and decoction treatment groups. SAP was induced by a retrograde pancreatic duct injection of 5% sodium taurocholate in the SAP and decoction treatment groups. Rats in decoction treatment group also received intragastric administration of Lai Fu Cheng Qi decoction. The serum levels of creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) were detected using an automatic biochemical analyzer. In addition, myocardial Na+-K+-ATPase activity was analyzed using a spectrophotometric method and the mitochondrial membrane potential was measured by flow cytometry. Myocardial apoptosis was assessed using a TUNEL assay and pathological changes to the heart and pancreas were detected by hematoxylin and eosin staining. Compared with the rats in the sham group, rats in the SAP and decoction treatment groups exhibited significantly higher levels of serum CK-MB and LDH, apoptosis index and pathological scores, and had significantly lower levels of Na+-K+-ATPase activity and mitochondrial membrane potential. However, when compared with the SAP group, the serum levels of CK-MB and LDH, the pathological scores of the pancreas and heart, and the myocardial cell apoptosis index in the decoction treatment group were significantly lower. Furthermore, the Na+-K+-ATPase activity and mitochondrial membrane potential were significantly increased in the decoction treatment group when compared with the SAP group. Therefore, Lai Fu Cheng Qi decoction was shown to exert a protective effect on myocardial injury induced by SAP in rats.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2015.2250</identifier><identifier>PMID: 25780399</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Abdomen ; Animals ; Apoptosis ; Bacteria ; Bile ; Biotechnology ; Care and treatment ; Chinese medicine ; Complications and side effects ; Gangrene ; Heart ; Heart diseases ; Injuries ; Ischemia ; Lai Fu Cheng Qi decoction ; Medical research ; Medicine, Chinese ; Medicine, Experimental ; myocardial protection ; Pancreas ; Pancreatitis ; Pathogenesis ; rats ; Rodents ; severe acute pancreatitis ; Sodium ; Standard deviation ; Studies</subject><ispartof>Experimental and therapeutic medicine, 2015-04, Vol.9 (4), p.1133-1140</ispartof><rights>Copyright © 2015, Spandidos Publications</rights><rights>COPYRIGHT 2015 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2015</rights><rights>Copyright © 2015, Spandidos Publications 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-2466f7cbf62b243fa32238f21d0191feb87faf29c40ca4a1bfc903e5070aa6d3</citedby><cites>FETCH-LOGICAL-c514t-2466f7cbf62b243fa32238f21d0191feb87faf29c40ca4a1bfc903e5070aa6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353776/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353776/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25780399$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LI, NAN</creatorcontrib><creatorcontrib>TIAN, YING</creatorcontrib><creatorcontrib>WANG, CHUNLI</creatorcontrib><creatorcontrib>ZHANG, PENG</creatorcontrib><creatorcontrib>YOU, SHENGYI</creatorcontrib><title>Protective effect of Lai Fu Cheng Qi decoction on severe acute pancreatitis-induced myocardial injury in a rat model</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>The aim of the present study was to evaluate the effects of Lai Fu Cheng Qi decoction on myocardial injury in rats with severe acute pancreatitis (SAP). In total, 30 rats were randomly divided into sham, SAP and decoction treatment groups. SAP was induced by a retrograde pancreatic duct injection of 5% sodium taurocholate in the SAP and decoction treatment groups. Rats in decoction treatment group also received intragastric administration of Lai Fu Cheng Qi decoction. The serum levels of creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) were detected using an automatic biochemical analyzer. In addition, myocardial Na+-K+-ATPase activity was analyzed using a spectrophotometric method and the mitochondrial membrane potential was measured by flow cytometry. Myocardial apoptosis was assessed using a TUNEL assay and pathological changes to the heart and pancreas were detected by hematoxylin and eosin staining. Compared with the rats in the sham group, rats in the SAP and decoction treatment groups exhibited significantly higher levels of serum CK-MB and LDH, apoptosis index and pathological scores, and had significantly lower levels of Na+-K+-ATPase activity and mitochondrial membrane potential. However, when compared with the SAP group, the serum levels of CK-MB and LDH, the pathological scores of the pancreas and heart, and the myocardial cell apoptosis index in the decoction treatment group were significantly lower. Furthermore, the Na+-K+-ATPase activity and mitochondrial membrane potential were significantly increased in the decoction treatment group when compared with the SAP group. Therefore, Lai Fu Cheng Qi decoction was shown to exert a protective effect on myocardial injury induced by SAP in rats.</description><subject>Abdomen</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Bacteria</subject><subject>Bile</subject><subject>Biotechnology</subject><subject>Care and treatment</subject><subject>Chinese medicine</subject><subject>Complications and side effects</subject><subject>Gangrene</subject><subject>Heart</subject><subject>Heart diseases</subject><subject>Injuries</subject><subject>Ischemia</subject><subject>Lai Fu Cheng Qi decoction</subject><subject>Medical research</subject><subject>Medicine, Chinese</subject><subject>Medicine, Experimental</subject><subject>myocardial protection</subject><subject>Pancreas</subject><subject>Pancreatitis</subject><subject>Pathogenesis</subject><subject>rats</subject><subject>Rodents</subject><subject>severe acute pancreatitis</subject><subject>Sodium</subject><subject>Standard deviation</subject><subject>Studies</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptkk2LFDEQhhtR3GXdo1cJeNBLj_noj-QiLIOrwoAKew_ppDKboTsZk_TA_HvTzDi6YhJISJ56Q1W9VfWa4BXjgn6APK0oJu2K0hY_q65JL2hNysXz8xkLTq6q25R2uIy2I5y3L6sr2vYcMyGuq_w9hgw6uwMgsLacULBooxy6n9H6EfwW_XDIgA6FCR6VleAAEZDScwa0V15HUNlll2rnzazBoOkYtIrGqRE5v5vjsWxIoagymoKB8VX1wqoxwe15v6ke7j89rL_Um2-fv67vNrVuSZNr2nSd7fVgOzrQhlnFKGXcUmIwEcTCwHurLBW6wVo1igxWC8ygxT1WqjPspvp4kt3PwwRGg89RjXIf3aTiUQbl5NMX7x7lNhxkw1rW910ReH8WiOHnDCnLySUN46g8hDlJwmnXlfqLBX37D7oLc_QlO0kEKy0RtGv-UFs1gnTehvKvXkTlXYN5z0tXeaFW_6HKNDA5HTxYV-6fBNSnAB1DShHsJUeC5WIUWYwiF6PIxSiFf_N3YS70b1sU4N0JSKW_xpmQLkxRKq6qcVMTwhj7BWlPxgM</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>LI, NAN</creator><creator>TIAN, YING</creator><creator>WANG, CHUNLI</creator><creator>ZHANG, PENG</creator><creator>YOU, SHENGYI</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150401</creationdate><title>Protective effect of Lai Fu Cheng Qi decoction on severe acute pancreatitis-induced myocardial injury in a rat model</title><author>LI, NAN ; TIAN, YING ; WANG, CHUNLI ; ZHANG, PENG ; YOU, SHENGYI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-2466f7cbf62b243fa32238f21d0191feb87faf29c40ca4a1bfc903e5070aa6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Abdomen</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Bacteria</topic><topic>Bile</topic><topic>Biotechnology</topic><topic>Care and treatment</topic><topic>Chinese medicine</topic><topic>Complications and side effects</topic><topic>Gangrene</topic><topic>Heart</topic><topic>Heart diseases</topic><topic>Injuries</topic><topic>Ischemia</topic><topic>Lai Fu Cheng Qi decoction</topic><topic>Medical research</topic><topic>Medicine, Chinese</topic><topic>Medicine, Experimental</topic><topic>myocardial protection</topic><topic>Pancreas</topic><topic>Pancreatitis</topic><topic>Pathogenesis</topic><topic>rats</topic><topic>Rodents</topic><topic>severe acute pancreatitis</topic><topic>Sodium</topic><topic>Standard deviation</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LI, NAN</creatorcontrib><creatorcontrib>TIAN, YING</creatorcontrib><creatorcontrib>WANG, CHUNLI</creatorcontrib><creatorcontrib>ZHANG, PENG</creatorcontrib><creatorcontrib>YOU, SHENGYI</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LI, NAN</au><au>TIAN, YING</au><au>WANG, CHUNLI</au><au>ZHANG, PENG</au><au>YOU, SHENGYI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effect of Lai Fu Cheng Qi decoction on severe acute pancreatitis-induced myocardial injury in a rat model</atitle><jtitle>Experimental and therapeutic medicine</jtitle><addtitle>Exp Ther Med</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>9</volume><issue>4</issue><spage>1133</spage><epage>1140</epage><pages>1133-1140</pages><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>The aim of the present study was to evaluate the effects of Lai Fu Cheng Qi decoction on myocardial injury in rats with severe acute pancreatitis (SAP). In total, 30 rats were randomly divided into sham, SAP and decoction treatment groups. SAP was induced by a retrograde pancreatic duct injection of 5% sodium taurocholate in the SAP and decoction treatment groups. Rats in decoction treatment group also received intragastric administration of Lai Fu Cheng Qi decoction. The serum levels of creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) were detected using an automatic biochemical analyzer. In addition, myocardial Na+-K+-ATPase activity was analyzed using a spectrophotometric method and the mitochondrial membrane potential was measured by flow cytometry. Myocardial apoptosis was assessed using a TUNEL assay and pathological changes to the heart and pancreas were detected by hematoxylin and eosin staining. Compared with the rats in the sham group, rats in the SAP and decoction treatment groups exhibited significantly higher levels of serum CK-MB and LDH, apoptosis index and pathological scores, and had significantly lower levels of Na+-K+-ATPase activity and mitochondrial membrane potential. However, when compared with the SAP group, the serum levels of CK-MB and LDH, the pathological scores of the pancreas and heart, and the myocardial cell apoptosis index in the decoction treatment group were significantly lower. Furthermore, the Na+-K+-ATPase activity and mitochondrial membrane potential were significantly increased in the decoction treatment group when compared with the SAP group. Therefore, Lai Fu Cheng Qi decoction was shown to exert a protective effect on myocardial injury induced by SAP in rats.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>25780399</pmid><doi>10.3892/etm.2015.2250</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Abdomen Animals Apoptosis Bacteria Bile Biotechnology Care and treatment Chinese medicine Complications and side effects Gangrene Heart Heart diseases Injuries Ischemia Lai Fu Cheng Qi decoction Medical research Medicine, Chinese Medicine, Experimental myocardial protection Pancreas Pancreatitis Pathogenesis rats Rodents severe acute pancreatitis Sodium Standard deviation Studies |
| title | Protective effect of Lai Fu Cheng Qi decoction on severe acute pancreatitis-induced myocardial injury in a rat model |
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