Recombinase polymerase amplification-based assay to diagnose Giardia in stool samples
Giardia duodenalis is one of the most commonly identified parasites in stool samples. Although relatively easy to treat, giardiasis can be difficult to detect as it presents similar to other diarrheal diseases. Here, we present a recombinase polymerase amplification-based Giardia (RPAG) assay to det...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2015-03, Vol.92 (3), p.583-587 |
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| container_title | The American journal of tropical medicine and hygiene |
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| creator | Crannell, Zachary Austin Cabada, Miguel Mauricio Castellanos-Gonzalez, Alejandro Irani, Ayesha White, Arthur Clinton Richards-Kortum, Rebecca |
| description | Giardia duodenalis is one of the most commonly identified parasites in stool samples. Although relatively easy to treat, giardiasis can be difficult to detect as it presents similar to other diarrheal diseases. Here, we present a recombinase polymerase amplification-based Giardia (RPAG) assay to detect the presence of Giardia in stool samples. The RPAG assay was characterized on the bench top using stool samples spiked with Giardia cysts where it showed a limit-of-detection nearly as low as the gold standard polymerase chain reaction assay. The RPAG assay was then tested in the highlands of Peru on 104 stool samples collected from the surrounding communities where it showed 73% sensitivity and 95% specificity against a polymerase chain reaction and microscopy composite gold standard. Further improvements in clinical sensitivity will be needed for the RPAG assay to have clinical relevance. |
| doi_str_mv | 10.4269/ajtmh.14-0593 |
| format | Article |
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Although relatively easy to treat, giardiasis can be difficult to detect as it presents similar to other diarrheal diseases. Here, we present a recombinase polymerase amplification-based Giardia (RPAG) assay to detect the presence of Giardia in stool samples. The RPAG assay was characterized on the bench top using stool samples spiked with Giardia cysts where it showed a limit-of-detection nearly as low as the gold standard polymerase chain reaction assay. The RPAG assay was then tested in the highlands of Peru on 104 stool samples collected from the surrounding communities where it showed 73% sensitivity and 95% specificity against a polymerase chain reaction and microscopy composite gold standard. Further improvements in clinical sensitivity will be needed for the RPAG assay to have clinical relevance.</description><identifier>ISSN: 0002-9637</identifier><identifier>EISSN: 1476-1645</identifier><identifier>DOI: 10.4269/ajtmh.14-0593</identifier><identifier>PMID: 25510713</identifier><language>eng</language><publisher>United States: The American Society of Tropical Medicine and Hygiene</publisher><subject>Feces - parasitology ; Giardia ; Giardia duodenalis ; Giardia lamblia - isolation & purification ; Giardiasis - diagnosis ; Giardiasis - epidemiology ; Humans ; Peru - epidemiology ; Polymerase Chain Reaction - methods ; Recombinases - chemistry ; Recombinases - metabolism ; Sensitivity and Specificity</subject><ispartof>The American journal of tropical medicine and hygiene, 2015-03, Vol.92 (3), p.583-587</ispartof><rights>The American Society of Tropical Medicine and Hygiene.</rights><rights>The American Society of Tropical Medicine and Hygiene 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-1f5d894fe3af31bd06d3bca77e1f4ca049330e087202215a5cc4a676b9bb6dc03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350554/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350554/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25510713$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Crannell, Zachary Austin</creatorcontrib><creatorcontrib>Cabada, Miguel Mauricio</creatorcontrib><creatorcontrib>Castellanos-Gonzalez, Alejandro</creatorcontrib><creatorcontrib>Irani, Ayesha</creatorcontrib><creatorcontrib>White, Arthur Clinton</creatorcontrib><creatorcontrib>Richards-Kortum, Rebecca</creatorcontrib><title>Recombinase polymerase amplification-based assay to diagnose Giardia in stool samples</title><title>The American journal of tropical medicine and hygiene</title><addtitle>Am J Trop Med Hyg</addtitle><description>Giardia duodenalis is one of the most commonly identified parasites in stool samples. 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| subjects | Feces - parasitology Giardia Giardia duodenalis Giardia lamblia - isolation & purification Giardiasis - diagnosis Giardiasis - epidemiology Humans Peru - epidemiology Polymerase Chain Reaction - methods Recombinases - chemistry Recombinases - metabolism Sensitivity and Specificity |
| title | Recombinase polymerase amplification-based assay to diagnose Giardia in stool samples |
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