Both tails and the centromere targeting domain of CENP-A are required for centromere establishment
The centromere-defined by the presence of nucleosomes containing the histone H3 variant, CENP-A-is the chromosomal locus required for the accurate segregation of chromosomes during cell division. Although the sequence determinants of human CENP-A required to maintain a centromere were reported, thos...
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| Veröffentlicht in: | The Journal of cell biology 2015-03, Vol.208 (5), p.521-531 |
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| container_title | The Journal of cell biology |
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| creator | Logsdon, Glennis A Barrey, Evelyne J Bassett, Emily A DeNizio, Jamie E Guo, Lucie Y Panchenko, Tanya Dawicki-McKenna, Jennine M Heun, Patrick Black, Ben E |
| description | The centromere-defined by the presence of nucleosomes containing the histone H3 variant, CENP-A-is the chromosomal locus required for the accurate segregation of chromosomes during cell division. Although the sequence determinants of human CENP-A required to maintain a centromere were reported, those that are required for early steps in establishing a new centromere are unknown. In this paper, we used gain-of-function histone H3 chimeras containing various regions unique to CENP-A to investigate early events in centromere establishment. We targeted histone H3 chimeras to chromosomally integrated Lac operator sequences by fusing each of the chimeras to the Lac repressor. Using this approach, we found surprising contributions from a small portion of the N-terminal tail and the CENP-A targeting domain in the initial recruitment of two essential constitutive centromere proteins, CENP-C and CENP-T. Our results indicate that the regions of CENP-A required for early events in centromere establishment differ from those that are required for maintaining centromere identity. |
| doi_str_mv | 10.1083/jcb.201412011 |
| format | Article |
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Although the sequence determinants of human CENP-A required to maintain a centromere were reported, those that are required for early steps in establishing a new centromere are unknown. In this paper, we used gain-of-function histone H3 chimeras containing various regions unique to CENP-A to investigate early events in centromere establishment. We targeted histone H3 chimeras to chromosomally integrated Lac operator sequences by fusing each of the chimeras to the Lac repressor. Using this approach, we found surprising contributions from a small portion of the N-terminal tail and the CENP-A targeting domain in the initial recruitment of two essential constitutive centromere proteins, CENP-C and CENP-T. 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Our results indicate that the regions of CENP-A required for early events in centromere establishment differ from those that are required for maintaining centromere identity.</description><subject>Autoantigens - genetics</subject><subject>Autoantigens - metabolism</subject><subject>Cell division</subject><subject>Cell Line, Tumor</subject><subject>Centromere - genetics</subject><subject>Centromere - metabolism</subject><subject>Centromere Protein A</subject><subject>Chromosomal Proteins, Non-Histone - genetics</subject><subject>Chromosomal Proteins, Non-Histone - metabolism</subject><subject>Chromosomes</subject><subject>Histones - genetics</subject><subject>Histones - metabolism</subject><subject>Humans</subject><subject>Protein Structure, Tertiary</subject><subject>Proteins</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1P3DAQxa2Kqmxpj1yRJS69hPpj7CQXJFhBWwm1PbRna5LM7nqVxGAnSPz39QJdQU-9eCS_3zzNzGPsWIozKSr9eds2Z0pIkPmRb9hCGhBFJUEcsIUQSha1UeaQvU9pK4SAEvQ7dqhMKTVIvWDNZZg2fELfJ45jx6cN8ZbGKYaBImUhrmny45p3YUA_8rDiy6vvP4sLjlmOdDf7SB1fhfiyjdKETe_TZsh_H9jbFfaJPj7XI_b7-urX8mtx8-PLt-XFTdEaCVOhlMbOCECJra2wbmQLhA1qTSDKEkiRtU0HBsBWNbRYa0NUd6oy2KFAfcTOn3xv52ag7nEc7N1t9APGBxfQu9fK6DduHe4daCgtiGzw6dkghrs57-AGn1rqexwpzMlJW1pttCnL_0CtsAa0khk9_QfdhjmO-RI7SgHUwu4MiyeqjSGlSKv93FK4XdAuB-32QWf-5OWye_pvsvoPupuj8g</recordid><startdate>20150302</startdate><enddate>20150302</enddate><creator>Logsdon, Glennis A</creator><creator>Barrey, Evelyne J</creator><creator>Bassett, Emily A</creator><creator>DeNizio, Jamie E</creator><creator>Guo, Lucie Y</creator><creator>Panchenko, Tanya</creator><creator>Dawicki-McKenna, Jennine M</creator><creator>Heun, Patrick</creator><creator>Black, Ben E</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150302</creationdate><title>Both tails and the centromere targeting domain of CENP-A are required for centromere establishment</title><author>Logsdon, Glennis A ; 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| subjects | Autoantigens - genetics Autoantigens - metabolism Cell division Cell Line, Tumor Centromere - genetics Centromere - metabolism Centromere Protein A Chromosomal Proteins, Non-Histone - genetics Chromosomal Proteins, Non-Histone - metabolism Chromosomes Histones - genetics Histones - metabolism Humans Protein Structure, Tertiary Proteins |
| title | Both tails and the centromere targeting domain of CENP-A are required for centromere establishment |
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