Coverage recommendations for methylation analysis by whole-genome bisulfite sequencing

Results show the minimum sequencing depth required for the discovery of differentially methylated regions at desired sensitivity and specificity and the trade-off between adding more replicates versus increasing sequencing depth. Whole-genome bisulfite sequencing (WGBS) allows genome-wide DNA methyl...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature methods 2015-03, Vol.12 (3), p.230-232
Hauptverfasser:	Ziller, Michael J, Hansen, Kasper D, Meissner, Alexander, Aryee, Martin J
Format:	Artikel
Sprache:	eng
Schlagworte:	631/114/2415 631/1647/2210/2213 Bioinformatics Biological Microscopy Biological Techniques Biomedical Engineering/Biotechnology Brain - physiology brief-communication CD4-Positive T-Lymphocytes - physiology CD8-Positive T-Lymphocytes - physiology Cellular biology CpG Islands Data Interpretation, Statistical Databases, Genetic Deoxyribonucleic acid DNA DNA Methylation DNA sequencing Embryonic Stem Cells - physiology Gene expression Genetic research Genome, Human High-Throughput Nucleotide Sequencing Humans Innovations Life Sciences Nucleotide sequencing Proteomics Sensitivity and Specificity Sequence Analysis, DNA - methods Sulfites
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!