3′‐Box‐dependent processing of human pre‐U1 snRNA requires a combination of RNA and protein co‐factors

Using an in vitro system we have recently shown that the 3′ ends of human pre‐snRNAs synthesized by RNA polymerase II are produced by RNA processing directed by the snRNA gene‐specific 3′ box. Towards a complete characterization of this processing reaction we have further investigated the in vitro r...

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Veröffentlicht in:	Nucleic acids research 2004, Vol.32 (10), p.2987-2994
Hauptverfasser:	Uguen, Patricia, Murphy, Shona
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism Base Sequence Binding Sites Coenzymes - metabolism Enzyme Stability HeLa Cells Hot Temperature Humans Molecular Sequence Data Nucleic Acid Conformation Phosphocreatine - metabolism Ribonucleoproteins - chemistry Ribonucleoproteins - genetics Ribonucleoproteins - metabolism RNA Caps - chemistry RNA Caps - genetics RNA Caps - metabolism RNA Processing, Post-Transcriptional RNA, Small Nuclear - chemistry RNA, Small Nuclear - genetics RNA, Small Nuclear - metabolism
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