Twenty Years of Acanthamoeba Diagnostics in Austria
Acanthamoebae are the causative agents of an often seriously progressing keratitis (AK) occurring predominantly in contact lens wearers and can cause several disseminating infections potentially resulting in granulomatous amoebic encephalitis (GAE) in the immunocompromised host. Our institution is t...
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| Veröffentlicht in: | The Journal of eukaryotic microbiology 2015-01, Vol.62 (1), p.3-11 |
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| creator | Walochnik, Julia Scheikl, Ute Haller‐Schober, Eva‐Maria |
| description | Acanthamoebae are the causative agents of an often seriously progressing keratitis (AK) occurring predominantly in contact lens wearers and can cause several disseminating infections potentially resulting in granulomatous amoebic encephalitis (GAE) in the immunocompromised host. Our institution is the Austrian reference laboratory for Acanthamoeba diagnostics and the aim of this study was to give an overview of proven cases of Acanthamoeba infections in Austria during the past 20 yr. All samples of patients with suspected AK or GAE were screened for Acanthamoeba spp. by culture and/or PCR and the detected amoebae were genotyped. Altogether, 154 cases of AK and three cases of GAE were diagnosed. Age of the AK patients ranged from 8 to 82 yr (mean 37.8) and 58% of the patients were female. Approximately 89% of the AK patients were contact lens wearers, almost all cases were unilateral and 19% of the patients required a keratoplasty. Age of the GAE patients ranged from 2 to 25 yr (mean 14.7), all were HIV‐negative, but two were severely immunosuppressed at the time of diagnosis. The predominant genotype in the AK cases was T4, other genotypes found were T3, T5, T6, T10 and T11. The three GAE cases involved genotypes T2, T4 and T5. |
| doi_str_mv | 10.1111/jeu.12149 |
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Our institution is the Austrian reference laboratory for Acanthamoeba diagnostics and the aim of this study was to give an overview of proven cases of Acanthamoeba infections in Austria during the past 20 yr. All samples of patients with suspected AK or GAE were screened for Acanthamoeba spp. by culture and/or PCR and the detected amoebae were genotyped. Altogether, 154 cases of AK and three cases of GAE were diagnosed. Age of the AK patients ranged from 8 to 82 yr (mean 37.8) and 58% of the patients were female. Approximately 89% of the AK patients were contact lens wearers, almost all cases were unilateral and 19% of the patients required a keratoplasty. Age of the GAE patients ranged from 2 to 25 yr (mean 14.7), all were HIV‐negative, but two were severely immunosuppressed at the time of diagnosis. The predominant genotype in the AK cases was T4, other genotypes found were T3, T5, T6, T10 and T11. The three GAE cases involved genotypes T2, T4 and T5.</description><identifier>ISSN: 1066-5234</identifier><identifier>EISSN: 1550-7408</identifier><identifier>DOI: 10.1111/jeu.12149</identifier><identifier>PMID: 25047131</identifier><language>eng</language><publisher>United States: Society of Protozoologists</publisher><subject>Acanthamoeba ; Acanthamoeba - classification ; Acanthamoeba - genetics ; Acanthamoeba - isolation & purification ; Acanthamoeba Keratitis - diagnosis ; Acanthamoeba Keratitis - immunology ; Acanthamoeba Keratitis - parasitology ; Acanthamoeba Keratitis - surgery ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Amebiasis - diagnosis ; Amebiasis - immunology ; Amebiasis - parasitology ; Amebiasis - surgery ; characterisation ; Child ; Child, Preschool ; contact lens ; Contact Lenses - parasitology ; Corneal Transplantation - methods ; diagnostic techniques ; encephalitis ; Encephalitis - diagnosis ; Encephalitis - immunology ; Encephalitis - parasitology ; Encephalitis - surgery ; Female ; females ; Genotype ; genotyping ; Humans ; Immunocompromised Host ; infection ; keratitis ; Male ; Middle Aged ; Original ; patients ; polymerase chain reaction ; protozoa ; SSU ; typing</subject><ispartof>The Journal of eukaryotic microbiology, 2015-01, Vol.62 (1), p.3-11</ispartof><rights>2014 The Authors. The published by Wiley Periodicals, Inc. on behalf of International Society of Protistologists</rights><rights>2014 The Authors. The Journal of Eukaryotic Microbiology published by Wiley Periodicals, Inc. on behalf of International Society of Protistologists.</rights><rights>2014 The Authors. The published by Wiley Periodicals, Inc. on behalf of International Society of Protistologists 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5479-7d1a9bb4f55b878e8bdf77afe89784a7e5c50f0668e5d75e8995e9431d361673</citedby><cites>FETCH-LOGICAL-c5479-7d1a9bb4f55b878e8bdf77afe89784a7e5c50f0668e5d75e8995e9431d361673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjeu.12149$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjeu.12149$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25047131$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Walochnik, Julia</creatorcontrib><creatorcontrib>Scheikl, Ute</creatorcontrib><creatorcontrib>Haller‐Schober, Eva‐Maria</creatorcontrib><title>Twenty Years of Acanthamoeba Diagnostics in Austria</title><title>The Journal of eukaryotic microbiology</title><addtitle>J. Eukaryot. Microbiol</addtitle><description>Acanthamoebae are the causative agents of an often seriously progressing keratitis (AK) occurring predominantly in contact lens wearers and can cause several disseminating infections potentially resulting in granulomatous amoebic encephalitis (GAE) in the immunocompromised host. Our institution is the Austrian reference laboratory for Acanthamoeba diagnostics and the aim of this study was to give an overview of proven cases of Acanthamoeba infections in Austria during the past 20 yr. All samples of patients with suspected AK or GAE were screened for Acanthamoeba spp. by culture and/or PCR and the detected amoebae were genotyped. Altogether, 154 cases of AK and three cases of GAE were diagnosed. Age of the AK patients ranged from 8 to 82 yr (mean 37.8) and 58% of the patients were female. Approximately 89% of the AK patients were contact lens wearers, almost all cases were unilateral and 19% of the patients required a keratoplasty. Age of the GAE patients ranged from 2 to 25 yr (mean 14.7), all were HIV‐negative, but two were severely immunosuppressed at the time of diagnosis. The predominant genotype in the AK cases was T4, other genotypes found were T3, T5, T6, T10 and T11. The three GAE cases involved genotypes T2, T4 and T5.</description><subject>Acanthamoeba</subject><subject>Acanthamoeba - classification</subject><subject>Acanthamoeba - genetics</subject><subject>Acanthamoeba - isolation & purification</subject><subject>Acanthamoeba Keratitis - diagnosis</subject><subject>Acanthamoeba Keratitis - immunology</subject><subject>Acanthamoeba Keratitis - parasitology</subject><subject>Acanthamoeba Keratitis - surgery</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Amebiasis - diagnosis</subject><subject>Amebiasis - immunology</subject><subject>Amebiasis - parasitology</subject><subject>Amebiasis - surgery</subject><subject>characterisation</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>contact lens</subject><subject>Contact Lenses - parasitology</subject><subject>Corneal Transplantation - methods</subject><subject>diagnostic techniques</subject><subject>encephalitis</subject><subject>Encephalitis - diagnosis</subject><subject>Encephalitis - immunology</subject><subject>Encephalitis - parasitology</subject><subject>Encephalitis - surgery</subject><subject>Female</subject><subject>females</subject><subject>Genotype</subject><subject>genotyping</subject><subject>Humans</subject><subject>Immunocompromised Host</subject><subject>infection</subject><subject>keratitis</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Original</subject><subject>patients</subject><subject>polymerase chain reaction</subject><subject>protozoa</subject><subject>SSU</subject><subject>typing</subject><issn>1066-5234</issn><issn>1550-7408</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp1kU1P3DAQhi1UBJRy6B9oc6SHgD_j5FJp2dKlFaJSWUCcRpPEWUyzMdhJ6f57TAOrcqgvtuxnHo_eIeQ9owcsrsNbMxwwzmSxQXaYUjTVkuZv4plmWaq4kNvkbQi3lLKMM7ZFtrmiUjPBdoiYP5iuXyXXBn1IXJNMKuz6G1w6U2LyxeKic6G3VUhsl0yG0HuL78hmg20we8_7Lpl_PZ5PT9LTH7Nv08lpWimpi1TXDIuylI1SZa5zk5d1ozU2Ji90LlEbVSnaxBZzo2qt4nWhTCEFq0XGMi12yedRezeUS1NXsU-PLdx5u0S_AocWXr909gYW7jdIIbnOiijYfxZ4dz-Y0MPShsq0LXbGDQFYprjkjKsn9NOIVt6F4E2z_oZReMoYYsbwN-PIfvi3rzX5EmoEDkfgwbZm9X8TfD--eFGmY4UNvfmzrkD_C2IQWsHV2Qz40eXsUkxP4GfkP458gw5w4W2Ai3MexxtHzKNRiEcE8J3I</recordid><startdate>201501</startdate><enddate>201501</enddate><creator>Walochnik, Julia</creator><creator>Scheikl, Ute</creator><creator>Haller‐Schober, Eva‐Maria</creator><general>Society of Protozoologists</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201501</creationdate><title>Twenty Years of Acanthamoeba Diagnostics in Austria</title><author>Walochnik, Julia ; Scheikl, Ute ; Haller‐Schober, Eva‐Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5479-7d1a9bb4f55b878e8bdf77afe89784a7e5c50f0668e5d75e8995e9431d361673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acanthamoeba</topic><topic>Acanthamoeba - classification</topic><topic>Acanthamoeba - genetics</topic><topic>Acanthamoeba - isolation & purification</topic><topic>Acanthamoeba Keratitis - diagnosis</topic><topic>Acanthamoeba Keratitis - immunology</topic><topic>Acanthamoeba Keratitis - parasitology</topic><topic>Acanthamoeba Keratitis - surgery</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Amebiasis - diagnosis</topic><topic>Amebiasis - immunology</topic><topic>Amebiasis - parasitology</topic><topic>Amebiasis - surgery</topic><topic>characterisation</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>contact lens</topic><topic>Contact Lenses - parasitology</topic><topic>Corneal Transplantation - methods</topic><topic>diagnostic techniques</topic><topic>encephalitis</topic><topic>Encephalitis - diagnosis</topic><topic>Encephalitis - immunology</topic><topic>Encephalitis - parasitology</topic><topic>Encephalitis - surgery</topic><topic>Female</topic><topic>females</topic><topic>Genotype</topic><topic>genotyping</topic><topic>Humans</topic><topic>Immunocompromised Host</topic><topic>infection</topic><topic>keratitis</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Original</topic><topic>patients</topic><topic>polymerase chain reaction</topic><topic>protozoa</topic><topic>SSU</topic><topic>typing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Walochnik, Julia</creatorcontrib><creatorcontrib>Scheikl, Ute</creatorcontrib><creatorcontrib>Haller‐Schober, Eva‐Maria</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of eukaryotic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Walochnik, Julia</au><au>Scheikl, Ute</au><au>Haller‐Schober, Eva‐Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Twenty Years of Acanthamoeba Diagnostics in Austria</atitle><jtitle>The Journal of eukaryotic microbiology</jtitle><addtitle>J. Eukaryot. Microbiol</addtitle><date>2015-01</date><risdate>2015</risdate><volume>62</volume><issue>1</issue><spage>3</spage><epage>11</epage><pages>3-11</pages><issn>1066-5234</issn><eissn>1550-7408</eissn><abstract>Acanthamoebae are the causative agents of an often seriously progressing keratitis (AK) occurring predominantly in contact lens wearers and can cause several disseminating infections potentially resulting in granulomatous amoebic encephalitis (GAE) in the immunocompromised host. Our institution is the Austrian reference laboratory for Acanthamoeba diagnostics and the aim of this study was to give an overview of proven cases of Acanthamoeba infections in Austria during the past 20 yr. All samples of patients with suspected AK or GAE were screened for Acanthamoeba spp. by culture and/or PCR and the detected amoebae were genotyped. Altogether, 154 cases of AK and three cases of GAE were diagnosed. Age of the AK patients ranged from 8 to 82 yr (mean 37.8) and 58% of the patients were female. Approximately 89% of the AK patients were contact lens wearers, almost all cases were unilateral and 19% of the patients required a keratoplasty. Age of the GAE patients ranged from 2 to 25 yr (mean 14.7), all were HIV‐negative, but two were severely immunosuppressed at the time of diagnosis. The predominant genotype in the AK cases was T4, other genotypes found were T3, T5, T6, T10 and T11. The three GAE cases involved genotypes T2, T4 and T5.</abstract><cop>United States</cop><pub>Society of Protozoologists</pub><pmid>25047131</pmid><doi>10.1111/jeu.12149</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acanthamoeba Acanthamoeba - classification Acanthamoeba - genetics Acanthamoeba - isolation & purification Acanthamoeba Keratitis - diagnosis Acanthamoeba Keratitis - immunology Acanthamoeba Keratitis - parasitology Acanthamoeba Keratitis - surgery Adolescent Adult Aged Aged, 80 and over Amebiasis - diagnosis Amebiasis - immunology Amebiasis - parasitology Amebiasis - surgery characterisation Child Child, Preschool contact lens Contact Lenses - parasitology Corneal Transplantation - methods diagnostic techniques encephalitis Encephalitis - diagnosis Encephalitis - immunology Encephalitis - parasitology Encephalitis - surgery Female females Genotype genotyping Humans Immunocompromised Host infection keratitis Male Middle Aged Original patients polymerase chain reaction protozoa SSU typing |
| title | Twenty Years of Acanthamoeba Diagnostics in Austria |
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