A novel psychrophilic alkaline phosphatase from the metagenome of tidal flat sediments

Alkaline phosphatase (AP) catalyzes the hydrolytic cleavage of phosphate monoesters under alkaline conditions and plays important roles in microbial ecology and molecular biology applications. Here, we report on the first isolation and biochemical characterization of a thermolabile AP from a metagen...

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Veröffentlicht in:	BMC biotechnology 2015-01, Vol.15 (1), p.1-1, Article 1
Hauptverfasser:	Lee, Dae-Hee, Choi, Su-Lim, Rha, Eugene, Kim, Soo Jin, Yeom, Soo-Jin, Moon, Jae-Hee, Lee, Seung-Goo
Format:	Artikel
Sprache:	eng
Schlagworte:	alkaline phosphatase Alkaline Phosphatase - chemistry Alkaline Phosphatase - genetics Alkaline Phosphatase - metabolism Amino Acid Sequence amino acids calcium Calcium - metabolism catalytic activity Cloning, Molecular coasts dephosphorylation Enzyme Activation enzyme activity Enzyme Stability Escherichia coli esters Gene Library genes genomic libraries Geologic Sediments - microbiology glucose 1-phosphate heat inactivation Korean Peninsula Metagenome metagenomics microbial ecology molecular biology Molecular Sequence Data molecular weight nucleotides open reading frames phosphates Phylogeny phytic acid plasmids polypeptides Republic of Korea sediments Sequence Alignment signal peptide Substrate Specificity temperature
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