Cloning and expression of a distinctive class of self-incompatibility (S) gene from papaver rhoeas L
We present the identification, cloning, and characterization of a self-incompatibility (S) gene from Papaver rhoeas that has no significant homology to any previously reported gene sequences, including S genes from other species. This result suggests that a different self-incompatibility mechanism m...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1994-03, Vol.91 (6), p.2265-2269 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Foote, H.C.C Ride, J.P Franklin-Tong, V.E Walker, E.A Lawrence, M.J Franklin, F.C.H |
| description | We present the identification, cloning, and characterization of a self-incompatibility (S) gene from Papaver rhoeas that has no significant homology to any previously reported gene sequences, including S genes from other species. This result suggests that a different self-incompatibility mechanism may be operating in this species and has important implications for the evolutionary relationships between the S genes. The S1 cDNA was cloned by using an oligonucleotide based upon N-terminal amino acid sequence data from stigmatic proteins that show complete linkage with the S1 gene. The single-copy gene has been expressed in Escherichia coli to test biological activity. Although the recombinant S1 protein (S1e) is not processed in the same way as the protein produced in the plant, it exhibits, in vitro, the specific pollen inhibitory activity expected of an S gene product; pollen carrying the S1 allele is inhibited, whereas pollen not carrying S1 is not inhibited. These results provide definitive demonstration that the product of a cloned S gene has S-specific pollen inhibitory activity |
| doi_str_mv | 10.1073/pnas.91.6.2265 |
| format | Article |
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This result suggests that a different self-incompatibility mechanism may be operating in this species and has important implications for the evolutionary relationships between the S genes. The S1 cDNA was cloned by using an oligonucleotide based upon N-terminal amino acid sequence data from stigmatic proteins that show complete linkage with the S1 gene. The single-copy gene has been expressed in Escherichia coli to test biological activity. Although the recombinant S1 protein (S1e) is not processed in the same way as the protein produced in the plant, it exhibits, in vitro, the specific pollen inhibitory activity expected of an S gene product; pollen carrying the S1 allele is inhibited, whereas pollen not carrying S1 is not inhibited. These results provide definitive demonstration that the product of a cloned S gene has S-specific pollen inhibitory activity</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.91.6.2265</identifier><identifier>PMID: 8134385</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Alleles ; Amino Acid Sequence ; Amino acids ; AUTOESTERILIDAD ; AUTOSTERILITE ; Base Sequence ; Biological and medical sciences ; Biological Evolution ; Biology ; Blotting, Northern ; Blotting, Southern ; Botany ; Cloning, Molecular ; DNA ; Flowers & plants ; Fundamental and applied biological sciences. Psychology ; Gels ; GENE ; Gene Expression ; GENES ; Genes, Plant ; Genes. Genome ; GERMINACION ; GERMINATION ; GINECEO ; GYNECEE ; INHIBICION ; INHIBITION ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; PAPAVER ; Papaver - genetics ; Papaver - immunology ; Papaver rhoeas ; Papillae ; Plant Proteins - genetics ; Plants ; Plants, Medicinal ; POLEN ; POLLEN ; PROTEINAS ; PROTEINE ; Proteins ; SECUENCIA NUCLEICA ; SEQUENCE NUCLEIQUE ; Sequencing</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1994-03, Vol.91 (6), p.2265-2269</ispartof><rights>Copyright 1994 The National Academy of Sciences of the United States of America</rights><rights>1994 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Mar 15, 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c629t-5935386e7637fc18128dc141ca4c0015e4cbd20fea231fb77fb45798c24429fb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/91/6.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2364214$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2364214$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4116960$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8134385$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Foote, H.C.C</creatorcontrib><creatorcontrib>Ride, J.P</creatorcontrib><creatorcontrib>Franklin-Tong, V.E</creatorcontrib><creatorcontrib>Walker, E.A</creatorcontrib><creatorcontrib>Lawrence, M.J</creatorcontrib><creatorcontrib>Franklin, F.C.H</creatorcontrib><title>Cloning and expression of a distinctive class of self-incompatibility (S) gene from papaver rhoeas L</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We present the identification, cloning, and characterization of a self-incompatibility (S) gene from Papaver rhoeas that has no significant homology to any previously reported gene sequences, including S genes from other species. This result suggests that a different self-incompatibility mechanism may be operating in this species and has important implications for the evolutionary relationships between the S genes. The S1 cDNA was cloned by using an oligonucleotide based upon N-terminal amino acid sequence data from stigmatic proteins that show complete linkage with the S1 gene. The single-copy gene has been expressed in Escherichia coli to test biological activity. Although the recombinant S1 protein (S1e) is not processed in the same way as the protein produced in the plant, it exhibits, in vitro, the specific pollen inhibitory activity expected of an S gene product; pollen carrying the S1 allele is inhibited, whereas pollen not carrying S1 is not inhibited. These results provide definitive demonstration that the product of a cloned S gene has S-specific pollen inhibitory activity</description><subject>Alleles</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>AUTOESTERILIDAD</subject><subject>AUTOSTERILITE</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biological Evolution</subject><subject>Biology</subject><subject>Blotting, Northern</subject><subject>Blotting, Southern</subject><subject>Botany</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>Flowers & plants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>GENE</subject><subject>Gene Expression</subject><subject>GENES</subject><subject>Genes, Plant</subject><subject>Genes. Genome</subject><subject>GERMINACION</subject><subject>GERMINATION</subject><subject>GINECEO</subject><subject>GYNECEE</subject><subject>INHIBICION</subject><subject>INHIBITION</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>PAPAVER</subject><subject>Papaver - genetics</subject><subject>Papaver - immunology</subject><subject>Papaver rhoeas</subject><subject>Papillae</subject><subject>Plant Proteins - genetics</subject><subject>Plants</subject><subject>Plants, Medicinal</subject><subject>POLEN</subject><subject>POLLEN</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Proteins</subject><subject>SECUENCIA NUCLEICA</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>Sequencing</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1rFDEYhwdR6lq9ehCFICJ6mDXfMwEvZfELFjzUnkMmk2yzZJMxmV3a_74Zdh1aETwF8jzv--bNr6peIrhEsCGfhqDyUqAlX2LM2aNqgaBANacCPq4WEOKmbimmT6tnOW8hhIK18Kw6axGhpGWLql_5GFzYABV6YG6GZHJ2MYBogQK9y6MLenQHA7RXOU_X2Xhbl9u4G9ToOufdeAs-XH4EGxMMsCnuwKAGdTAJpOtoVAbr59UTq3w2L07neXX19cuv1fd6_fPbj9XFutYci7FmgjDSctNw0liNWoTbXiOKtKIaQsQM1V2PoTUKE2S7prEdZY1oNaYUC9uR8-rzse-w73am1yaMSXk5JLdT6VZG5eRDEty13MSDpIQwVMrfn8pT_L03eZQ7l7XxXgUT91k2nELGOPyviHgrGG9ZEd_-JW7jPoXyBxLDEgHiSBRpeZR0ijknY-cHIyinjOWUsRRIcjllXAre3F9z1k-hFv7uxFXWytukgnZ51ihCXDzYYmr_h85jpN17P5qb8d68f4qFvz7ybR5jmgVMOMWIFvzqiK2KUm1SecrVpWBlMwzJHXfm2Hw</recordid><startdate>19940315</startdate><enddate>19940315</enddate><creator>Foote, H.C.C</creator><creator>Ride, J.P</creator><creator>Franklin-Tong, V.E</creator><creator>Walker, E.A</creator><creator>Lawrence, M.J</creator><creator>Franklin, F.C.H</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19940315</creationdate><title>Cloning and expression of a distinctive class of self-incompatibility (S) gene from papaver rhoeas L</title><author>Foote, H.C.C ; Ride, J.P ; Franklin-Tong, V.E ; Walker, E.A ; Lawrence, M.J ; Franklin, F.C.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c629t-5935386e7637fc18128dc141ca4c0015e4cbd20fea231fb77fb45798c24429fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Alleles</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>AUTOESTERILIDAD</topic><topic>AUTOSTERILITE</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biological Evolution</topic><topic>Biology</topic><topic>Blotting, Northern</topic><topic>Blotting, Southern</topic><topic>Botany</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>Flowers & plants</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>GENE</topic><topic>Gene Expression</topic><topic>GENES</topic><topic>Genes, Plant</topic><topic>Genes. Genome</topic><topic>GERMINACION</topic><topic>GERMINATION</topic><topic>GINECEO</topic><topic>GYNECEE</topic><topic>INHIBICION</topic><topic>INHIBITION</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>PAPAVER</topic><topic>Papaver - genetics</topic><topic>Papaver - immunology</topic><topic>Papaver rhoeas</topic><topic>Papillae</topic><topic>Plant Proteins - genetics</topic><topic>Plants</topic><topic>Plants, Medicinal</topic><topic>POLEN</topic><topic>POLLEN</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Proteins</topic><topic>SECUENCIA NUCLEICA</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>Sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Foote, H.C.C</creatorcontrib><creatorcontrib>Ride, J.P</creatorcontrib><creatorcontrib>Franklin-Tong, V.E</creatorcontrib><creatorcontrib>Walker, E.A</creatorcontrib><creatorcontrib>Lawrence, M.J</creatorcontrib><creatorcontrib>Franklin, F.C.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Foote, H.C.C</au><au>Ride, J.P</au><au>Franklin-Tong, V.E</au><au>Walker, E.A</au><au>Lawrence, M.J</au><au>Franklin, F.C.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and expression of a distinctive class of self-incompatibility (S) gene from papaver rhoeas L</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1994-03-15</date><risdate>1994</risdate><volume>91</volume><issue>6</issue><spage>2265</spage><epage>2269</epage><pages>2265-2269</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>We present the identification, cloning, and characterization of a self-incompatibility (S) gene from Papaver rhoeas that has no significant homology to any previously reported gene sequences, including S genes from other species. This result suggests that a different self-incompatibility mechanism may be operating in this species and has important implications for the evolutionary relationships between the S genes. The S1 cDNA was cloned by using an oligonucleotide based upon N-terminal amino acid sequence data from stigmatic proteins that show complete linkage with the S1 gene. The single-copy gene has been expressed in Escherichia coli to test biological activity. Although the recombinant S1 protein (S1e) is not processed in the same way as the protein produced in the plant, it exhibits, in vitro, the specific pollen inhibitory activity expected of an S gene product; pollen carrying the S1 allele is inhibited, whereas pollen not carrying S1 is not inhibited. These results provide definitive demonstration that the product of a cloned S gene has S-specific pollen inhibitory activity</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8134385</pmid><doi>10.1073/pnas.91.6.2265</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1994-03, Vol.91 (6), p.2265-2269 |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Alleles Amino Acid Sequence Amino acids AUTOESTERILIDAD AUTOSTERILITE Base Sequence Biological and medical sciences Biological Evolution Biology Blotting, Northern Blotting, Southern Botany Cloning, Molecular DNA Flowers & plants Fundamental and applied biological sciences. Psychology Gels GENE Gene Expression GENES Genes, Plant Genes. Genome GERMINACION GERMINATION GINECEO GYNECEE INHIBICION INHIBITION Molecular and cellular biology Molecular genetics Molecular Sequence Data PAPAVER Papaver - genetics Papaver - immunology Papaver rhoeas Papillae Plant Proteins - genetics Plants Plants, Medicinal POLEN POLLEN PROTEINAS PROTEINE Proteins SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE Sequencing |
| title | Cloning and expression of a distinctive class of self-incompatibility (S) gene from papaver rhoeas L |
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