Efficient Multisite Unnatural Amino Acid Incorporation in Mammalian Cells via Optimized Pyrrolysyl tRNA Synthetase/tRNA Expression and Engineered eRF1

The efficient, site-specific introduction of unnatural amino acids into proteins in mammalian cells is an outstanding challenge in realizing the potential of genetic code expansion approaches. Addressing this challenge will allow the synthesis of modified recombinant proteins and augment emerging st...

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Veröffentlicht in:	Journal of the American Chemical Society 2014-11, Vol.136 (44), p.15577-15583
Hauptverfasser:	Schmied, Wolfgang H, Elsässer, Simon J, Uttamapinant, Chayasith, Chin, Jason W
Format:	Artikel
Sprache:	eng
Schlagworte:	amber amino acids Amino Acids - metabolism biopharmaceuticals genetic code image analysis Lysine-tRNA Ligase - chemistry Lysine-tRNA Ligase - metabolism mammals Pyrroles - chemistry recombinant proteins RNA, Transfer - metabolism stop codon transfer RNA translation (genetics)
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