Estimation of Costunolide and Dehydrocostus Lactone in Saussurea lappa and its Polyherbal Formulations followed by their Stability Studies Using HPLC-DAD
Saussurea lappa is one of the popular Ayurvedic herb; costunolide and dehydrocostus lactones are well-known sesquiterpene lactones contained in many plants used as popular herbs, such as S. lappa, and have been considered as potential candidates for the treatment of various types of tumor. The prese...
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| Veröffentlicht in: | Pharmacognosy Magazine 2015-01, Vol.11 (41), p.180-190 |
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| creator | Rao Vadaparthi, P R Kumar, K Sarma, Vanka Uma Maheswara Hussain, Qazi Altaf Babu, K Suresh |
| description | Saussurea lappa is one of the popular Ayurvedic herb; costunolide and dehydrocostus lactones are well-known sesquiterpene lactones contained in many plants used as popular herbs, such as S. lappa, and have been considered as potential candidates for the treatment of various types of tumor.
The present study was used for the quantification of costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations, stability studies of markers and characterization of their degradants.
HPLC analysis was performed on Waters NOVAPAK HR C18 column (300 mm × 3.9 mm i.d., 6 μm) using isocratic elution with acetonitrile and water (60:40% v/v).
The calibration curves of both analytes showed good linearity within the established range 5-100 μg/ml. The limits of detection (LOD) and quantification (LOQ) were 1.5 and 4.6 μg/ml for costunolide and 1.3 and 4.0 μg/ml for dehydrocostus lactone, respectively. Good results were achieved with respect to repeatability (%RSD < 2.0) and recovery (99.3-101.8%).
The method was found to be precise, accurate, specific, and was successfully used for analyzing costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations. The developed method was found to be suitable for stability studies of markers and characterization of their degradation products. |
| doi_str_mv | 10.4103/0973-1296.149736 |
| format | Article |
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The present study was used for the quantification of costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations, stability studies of markers and characterization of their degradants.
HPLC analysis was performed on Waters NOVAPAK HR C18 column (300 mm × 3.9 mm i.d., 6 μm) using isocratic elution with acetonitrile and water (60:40% v/v).
The calibration curves of both analytes showed good linearity within the established range 5-100 μg/ml. The limits of detection (LOD) and quantification (LOQ) were 1.5 and 4.6 μg/ml for costunolide and 1.3 and 4.0 μg/ml for dehydrocostus lactone, respectively. Good results were achieved with respect to repeatability (%RSD < 2.0) and recovery (99.3-101.8%).
The method was found to be precise, accurate, specific, and was successfully used for analyzing costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations. The developed method was found to be suitable for stability studies of markers and characterization of their degradation products.</description><identifier>ISSN: 0973-1296</identifier><identifier>EISSN: 0976-4062</identifier><identifier>DOI: 10.4103/0973-1296.149736</identifier><identifier>PMID: 25709231</identifier><language>eng</language><publisher>India: Medknow Publications and Media Pvt. Ltd</publisher><subject>Analysis ; Chemical properties ; Chromatography ; Health aspects ; High performance liquid chromatography ; Laboratories ; Lactones ; Membrane filters ; Methods ; Original ; Phytochemicals ; Quality standards ; Saussurea ; Studies</subject><ispartof>Pharmacognosy Magazine, 2015-01, Vol.11 (41), p.180-190</ispartof><rights>COPYRIGHT 2015 Medknow Publications and Media Pvt. Ltd.</rights><rights>Copyright Medknow Publications & Media Pvt Ltd Jan-Mar 2015</rights><rights>Copyright: © Pharmacognosy Magazine 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-71272546e2d9e2a4823757b5a45b1f9f82d99cfed8e0606dba381bc4ecefaef03</citedby><cites>FETCH-LOGICAL-c491t-71272546e2d9e2a4823757b5a45b1f9f82d99cfed8e0606dba381bc4ecefaef03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329622/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329622/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25709231$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rao Vadaparthi, P R</creatorcontrib><creatorcontrib>Kumar, K</creatorcontrib><creatorcontrib>Sarma, Vanka Uma Maheswara</creatorcontrib><creatorcontrib>Hussain, Qazi Altaf</creatorcontrib><creatorcontrib>Babu, K Suresh</creatorcontrib><title>Estimation of Costunolide and Dehydrocostus Lactone in Saussurea lappa and its Polyherbal Formulations followed by their Stability Studies Using HPLC-DAD</title><title>Pharmacognosy Magazine</title><addtitle>Pharmacogn Mag</addtitle><description>Saussurea lappa is one of the popular Ayurvedic herb; costunolide and dehydrocostus lactones are well-known sesquiterpene lactones contained in many plants used as popular herbs, such as S. lappa, and have been considered as potential candidates for the treatment of various types of tumor.
The present study was used for the quantification of costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations, stability studies of markers and characterization of their degradants.
HPLC analysis was performed on Waters NOVAPAK HR C18 column (300 mm × 3.9 mm i.d., 6 μm) using isocratic elution with acetonitrile and water (60:40% v/v).
The calibration curves of both analytes showed good linearity within the established range 5-100 μg/ml. The limits of detection (LOD) and quantification (LOQ) were 1.5 and 4.6 μg/ml for costunolide and 1.3 and 4.0 μg/ml for dehydrocostus lactone, respectively. Good results were achieved with respect to repeatability (%RSD < 2.0) and recovery (99.3-101.8%).
The method was found to be precise, accurate, specific, and was successfully used for analyzing costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations. The developed method was found to be suitable for stability studies of markers and characterization of their degradation products.</description><subject>Analysis</subject><subject>Chemical properties</subject><subject>Chromatography</subject><subject>Health aspects</subject><subject>High performance liquid chromatography</subject><subject>Laboratories</subject><subject>Lactones</subject><subject>Membrane filters</subject><subject>Methods</subject><subject>Original</subject><subject>Phytochemicals</subject><subject>Quality standards</subject><subject>Saussurea</subject><subject>Studies</subject><issn>0973-1296</issn><issn>0976-4062</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptUsuKFDEULURxHrp3JQE3bqpNUu-N0HTPQ2hwYJx1SCU33RlSSZukRupT_FtT3ePoiGSRy7nnnHBvTpa9I3hRElx8wl1T5IR29YKUqaxfZKcJqvMS1_TloT62T7KzEO4xrlqCm9fZCa0a3NGCnGY_L0LUA4_aWeQUWrkQR-uMloC4lWgNu0l6J2Y4oA0X0VlA2qJbPoYweuDI8P2eH8g6BnTjzLQD33ODLp0fRnOwDkg5Y9wPkKifUNyB9ug28l4bHadUjVJDQHdB2y26vtms8vVy_SZ7pbgJ8PbxPs_uLi--ra7zzderL6vlJhdlR2LeENrQqqyByg4oL1taNFXTV7yseqI61Sa8EwpkC7jGtex50ZJelCBAcVC4OM8-H333Yz-AFGCj54btfVqLn5jjmj3vWL1jW_fAyiJtltJk8PHRwLvvI4TIBh0EGMMtuDEwUldtSSmmdaJ--Id670Zv03gzi1SE0qb5w9pyA0xb5dK7YjZly6Lr6pY2uEqsxX9Y6UgYtEjfpHTCnwnwUSC8C8GDepqRYDbHic15YXNe2DFOSfL-7908CX7np_gFSk_HBA</recordid><startdate>201501</startdate><enddate>201501</enddate><creator>Rao Vadaparthi, P R</creator><creator>Kumar, K</creator><creator>Sarma, Vanka Uma Maheswara</creator><creator>Hussain, Qazi Altaf</creator><creator>Babu, K Suresh</creator><general>Medknow Publications and Media Pvt. 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The present study was used for the quantification of costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations, stability studies of markers and characterization of their degradants.
HPLC analysis was performed on Waters NOVAPAK HR C18 column (300 mm × 3.9 mm i.d., 6 μm) using isocratic elution with acetonitrile and water (60:40% v/v).
The calibration curves of both analytes showed good linearity within the established range 5-100 μg/ml. The limits of detection (LOD) and quantification (LOQ) were 1.5 and 4.6 μg/ml for costunolide and 1.3 and 4.0 μg/ml for dehydrocostus lactone, respectively. Good results were achieved with respect to repeatability (%RSD < 2.0) and recovery (99.3-101.8%).
The method was found to be precise, accurate, specific, and was successfully used for analyzing costunolide and dehydrocostus lactone in S. lappa and its polyherbal formulations. The developed method was found to be suitable for stability studies of markers and characterization of their degradation products.</abstract><cop>India</cop><pub>Medknow Publications and Media Pvt. Ltd</pub><pmid>25709231</pmid><doi>10.4103/0973-1296.149736</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Pharmacognosy Magazine, 2015-01, Vol.11 (41), p.180-190 |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Analysis Chemical properties Chromatography Health aspects High performance liquid chromatography Laboratories Lactones Membrane filters Methods Original Phytochemicals Quality standards Saussurea Studies |
| title | Estimation of Costunolide and Dehydrocostus Lactone in Saussurea lappa and its Polyherbal Formulations followed by their Stability Studies Using HPLC-DAD |
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