Augmentation of tumor necrosis factor family-induced apoptosis by E3330 in human hepatocellular carcinoma cell lines via inhibition of NF kappa B
To investigate the reduction of cell viability in human hepatocellular carcinoma (HCC) cell lines induced by inhibition of nuclear factor kappa B (NF kappa B). HLE, SKHep1, and HepG2 were incubated and E3330 was used to compare the stimulation of some chemotherapeutic drugs with that of TNF family,...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2005-10, Vol.11 (40), p.6258-6261 |
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| container_title | World journal of gastroenterology : WJG |
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| creator | Saitou, Yukiko Shiraki, Katsuya Yamanaka, Takenari Miyashita, Kazumi Inoue, Tomoko Yamanaka, Yutaka Yamaguchi, Yumi Enokimura, Naoyuki Yamamoto, Norihiko Itou, Keiichi Sugimoto, Kazushi Nakano, Takeshi |
| description | To investigate the reduction of cell viability in human hepatocellular carcinoma (HCC) cell lines induced by inhibition of nuclear factor kappa B (NF kappa B).
HLE, SKHep1, and HepG2 were incubated and E3330 was used to compare the stimulation of some chemotherapeutic drugs with that of TNF family, Fas ligand, TNF alpha and TNF-related apoptosis-inducing ligand (TRAIL) at the point of the reduction of cell viability by inhibiting NF kappa B.
E3330 decreased NF kappa B levels in HLE cells stimulated by TNF and TRAIL. The cytotoxicity of the combination of TRAIL, TNF alpha, Fas ligand, and E3330 increased synergistically in a dose-dependent manner compared to either E3330 alone in all HCC cell lines by MTT assay. However, the combination of some chemotherapeutic drugs and E3330 did not decrease the cell viability.
Inhibition of NF kappa B sensitizes human HCC cell lines to TNF-mediated apoptosis including TRAIL, and TRAIL-based tumor therapy might be a powerful potential therapeutic tool in the treatment of human HCC. |
| doi_str_mv | 10.3748/wjg.v11.i40.6258 |
| format | Article |
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HLE, SKHep1, and HepG2 were incubated and E3330 was used to compare the stimulation of some chemotherapeutic drugs with that of TNF family, Fas ligand, TNF alpha and TNF-related apoptosis-inducing ligand (TRAIL) at the point of the reduction of cell viability by inhibiting NF kappa B.
E3330 decreased NF kappa B levels in HLE cells stimulated by TNF and TRAIL. The cytotoxicity of the combination of TRAIL, TNF alpha, Fas ligand, and E3330 increased synergistically in a dose-dependent manner compared to either E3330 alone in all HCC cell lines by MTT assay. However, the combination of some chemotherapeutic drugs and E3330 did not decrease the cell viability.
Inhibition of NF kappa B sensitizes human HCC cell lines to TNF-mediated apoptosis including TRAIL, and TRAIL-based tumor therapy might be a powerful potential therapeutic tool in the treatment of human HCC.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v11.i40.6258</identifier><identifier>PMID: 16419152</identifier><language>eng</language><publisher>United States: First Department of Internal Medicine, Mie University School of Medicine, Tsu, Mie 514-8507,Japan</publisher><subject>Apoptosis - physiology ; Apoptosis Regulatory Proteins - metabolism ; Benzoquinones - pharmacology ; Carcinoma, Hepatocellular - metabolism ; Cell Line, Tumor ; Cell Proliferation ; Cell Survival ; Genes, Reporter ; Humans ; Liver Cancer ; Liver Neoplasms - metabolism ; Membrane Glycoproteins - metabolism ; NF-kappa B - antagonists & inhibitors ; NF-kappa B - metabolism ; Propionates - pharmacology ; TNF-Related Apoptosis-Inducing Ligand ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>World journal of gastroenterology : WJG, 2005-10, Vol.11 (40), p.6258-6261</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2005 Baishideng Publishing Group Inc. All rights reserved. 2005</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-c1fb3237e630d8bc65e809e6ae09bd31a5d9e99b344d69337c87be6db1e82a0e3</citedby><cites>FETCH-LOGICAL-c423t-c1fb3237e630d8bc65e809e6ae09bd31a5d9e99b344d69337c87be6db1e82a0e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/wjg/wjg.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320327/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320327/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16419152$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saitou, Yukiko</creatorcontrib><creatorcontrib>Shiraki, Katsuya</creatorcontrib><creatorcontrib>Yamanaka, Takenari</creatorcontrib><creatorcontrib>Miyashita, Kazumi</creatorcontrib><creatorcontrib>Inoue, Tomoko</creatorcontrib><creatorcontrib>Yamanaka, Yutaka</creatorcontrib><creatorcontrib>Yamaguchi, Yumi</creatorcontrib><creatorcontrib>Enokimura, Naoyuki</creatorcontrib><creatorcontrib>Yamamoto, Norihiko</creatorcontrib><creatorcontrib>Itou, Keiichi</creatorcontrib><creatorcontrib>Sugimoto, Kazushi</creatorcontrib><creatorcontrib>Nakano, Takeshi</creatorcontrib><title>Augmentation of tumor necrosis factor family-induced apoptosis by E3330 in human hepatocellular carcinoma cell lines via inhibition of NF kappa B</title><title>World journal of gastroenterology : WJG</title><addtitle>World J Gastroenterol</addtitle><description>To investigate the reduction of cell viability in human hepatocellular carcinoma (HCC) cell lines induced by inhibition of nuclear factor kappa B (NF kappa B).
HLE, SKHep1, and HepG2 were incubated and E3330 was used to compare the stimulation of some chemotherapeutic drugs with that of TNF family, Fas ligand, TNF alpha and TNF-related apoptosis-inducing ligand (TRAIL) at the point of the reduction of cell viability by inhibiting NF kappa B.
E3330 decreased NF kappa B levels in HLE cells stimulated by TNF and TRAIL. The cytotoxicity of the combination of TRAIL, TNF alpha, Fas ligand, and E3330 increased synergistically in a dose-dependent manner compared to either E3330 alone in all HCC cell lines by MTT assay. However, the combination of some chemotherapeutic drugs and E3330 did not decrease the cell viability.
Inhibition of NF kappa B sensitizes human HCC cell lines to TNF-mediated apoptosis including TRAIL, and TRAIL-based tumor therapy might be a powerful potential therapeutic tool in the treatment of human HCC.</description><subject>Apoptosis - physiology</subject><subject>Apoptosis Regulatory Proteins - metabolism</subject><subject>Benzoquinones - pharmacology</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Cell Survival</subject><subject>Genes, Reporter</subject><subject>Humans</subject><subject>Liver Cancer</subject><subject>Liver Neoplasms - metabolism</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>NF-kappa B - antagonists & inhibitors</subject><subject>NF-kappa B - metabolism</subject><subject>Propionates - pharmacology</subject><subject>TNF-Related Apoptosis-Inducing Ligand</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU1v1DAQtRCILoU7J-QD4pbt-CNfF6S2agGpopdytiaOs-slsYOdbLU_g3-Mwy5QLrY8896b8XuEvGWwFqWsLh53m_WesbWVsC54Xj0jK85ZnfFKwnOyYgBlVgtenpFXMe4AuBA5f0nOWCFZzXK-Ij8v581g3IST9Y76jk7z4AN1RgcfbaQd6im9Oxxsf8isa2dtWoqjH6ff_eZAb4QQQK2j23nAdJoRJ69N3889BqoxaOv8gHQp0d46E-neYiJsbWP_jP16S7_jOCK9ek1edNhH8-Z0n5NvtzcP15-zu_tPX64v7zItuZgyzbpGcFGaQkBbNbrITQW1KdBA3bSCYd7Wpq4bIWVb1EKUuiobU7QNMxVHMOKcfDzqjnMzmFYnEwL2agx2wHBQHq36v-PsVm38XknBIXmaBN4fBR7Rdeg2aufn4NLKKsXCAXIJADLBPpzmBP9jNnFSg42LF-iMn6MqaigFl3kCwhG4WB-D6f7uwkAtcS-6KsWtUtxqiTtR3j39wz_CKV_xC2epqkI</recordid><startdate>20051028</startdate><enddate>20051028</enddate><creator>Saitou, Yukiko</creator><creator>Shiraki, Katsuya</creator><creator>Yamanaka, Takenari</creator><creator>Miyashita, Kazumi</creator><creator>Inoue, Tomoko</creator><creator>Yamanaka, Yutaka</creator><creator>Yamaguchi, Yumi</creator><creator>Enokimura, Naoyuki</creator><creator>Yamamoto, Norihiko</creator><creator>Itou, Keiichi</creator><creator>Sugimoto, Kazushi</creator><creator>Nakano, Takeshi</creator><general>First Department of Internal Medicine, Mie University School of Medicine, Tsu, Mie 514-8507,Japan</general><general>Baishideng Publishing Group Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20051028</creationdate><title>Augmentation of tumor necrosis factor family-induced apoptosis by E3330 in human hepatocellular carcinoma cell lines via inhibition of NF kappa B</title><author>Saitou, Yukiko ; Shiraki, Katsuya ; Yamanaka, Takenari ; Miyashita, Kazumi ; Inoue, Tomoko ; Yamanaka, Yutaka ; Yamaguchi, Yumi ; Enokimura, Naoyuki ; Yamamoto, Norihiko ; Itou, Keiichi ; Sugimoto, Kazushi ; Nakano, Takeshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-c1fb3237e630d8bc65e809e6ae09bd31a5d9e99b344d69337c87be6db1e82a0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Apoptosis - physiology</topic><topic>Apoptosis Regulatory Proteins - metabolism</topic><topic>Benzoquinones - pharmacology</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Cell Survival</topic><topic>Genes, Reporter</topic><topic>Humans</topic><topic>Liver Cancer</topic><topic>Liver Neoplasms - metabolism</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>NF-kappa B - antagonists & inhibitors</topic><topic>NF-kappa B - metabolism</topic><topic>Propionates - pharmacology</topic><topic>TNF-Related Apoptosis-Inducing Ligand</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Saitou, Yukiko</creatorcontrib><creatorcontrib>Shiraki, Katsuya</creatorcontrib><creatorcontrib>Yamanaka, Takenari</creatorcontrib><creatorcontrib>Miyashita, Kazumi</creatorcontrib><creatorcontrib>Inoue, Tomoko</creatorcontrib><creatorcontrib>Yamanaka, Yutaka</creatorcontrib><creatorcontrib>Yamaguchi, Yumi</creatorcontrib><creatorcontrib>Enokimura, Naoyuki</creatorcontrib><creatorcontrib>Yamamoto, Norihiko</creatorcontrib><creatorcontrib>Itou, Keiichi</creatorcontrib><creatorcontrib>Sugimoto, Kazushi</creatorcontrib><creatorcontrib>Nakano, Takeshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saitou, Yukiko</au><au>Shiraki, Katsuya</au><au>Yamanaka, Takenari</au><au>Miyashita, Kazumi</au><au>Inoue, Tomoko</au><au>Yamanaka, Yutaka</au><au>Yamaguchi, Yumi</au><au>Enokimura, Naoyuki</au><au>Yamamoto, Norihiko</au><au>Itou, Keiichi</au><au>Sugimoto, Kazushi</au><au>Nakano, Takeshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Augmentation of tumor necrosis factor family-induced apoptosis by E3330 in human hepatocellular carcinoma cell lines via inhibition of NF kappa B</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World J Gastroenterol</addtitle><date>2005-10-28</date><risdate>2005</risdate><volume>11</volume><issue>40</issue><spage>6258</spage><epage>6261</epage><pages>6258-6261</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>To investigate the reduction of cell viability in human hepatocellular carcinoma (HCC) cell lines induced by inhibition of nuclear factor kappa B (NF kappa B).
HLE, SKHep1, and HepG2 were incubated and E3330 was used to compare the stimulation of some chemotherapeutic drugs with that of TNF family, Fas ligand, TNF alpha and TNF-related apoptosis-inducing ligand (TRAIL) at the point of the reduction of cell viability by inhibiting NF kappa B.
E3330 decreased NF kappa B levels in HLE cells stimulated by TNF and TRAIL. The cytotoxicity of the combination of TRAIL, TNF alpha, Fas ligand, and E3330 increased synergistically in a dose-dependent manner compared to either E3330 alone in all HCC cell lines by MTT assay. However, the combination of some chemotherapeutic drugs and E3330 did not decrease the cell viability.
Inhibition of NF kappa B sensitizes human HCC cell lines to TNF-mediated apoptosis including TRAIL, and TRAIL-based tumor therapy might be a powerful potential therapeutic tool in the treatment of human HCC.</abstract><cop>United States</cop><pub>First Department of Internal Medicine, Mie University School of Medicine, Tsu, Mie 514-8507,Japan</pub><pmid>16419152</pmid><doi>10.3748/wjg.v11.i40.6258</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1007-9327 |
| ispartof | World journal of gastroenterology : WJG, 2005-10, Vol.11 (40), p.6258-6261 |
| issn | 1007-9327 2219-2840 |
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| source | MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Apoptosis - physiology Apoptosis Regulatory Proteins - metabolism Benzoquinones - pharmacology Carcinoma, Hepatocellular - metabolism Cell Line, Tumor Cell Proliferation Cell Survival Genes, Reporter Humans Liver Cancer Liver Neoplasms - metabolism Membrane Glycoproteins - metabolism NF-kappa B - antagonists & inhibitors NF-kappa B - metabolism Propionates - pharmacology TNF-Related Apoptosis-Inducing Ligand Tumor Necrosis Factor-alpha - metabolism |
| title | Augmentation of tumor necrosis factor family-induced apoptosis by E3330 in human hepatocellular carcinoma cell lines via inhibition of NF kappa B |
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