Dual-color labeled anti-mucin 1 antibody for imaging of ovarian cancer: A preliminary animal study
To investigate the feasibility of the anti-mucin 1 (anti-MUC1/CD227) antibody in the fluorescent imaging of ovarian cancer, the CD227 antibody and a control IgG antibody were labeled with a near-infrared dye [Cy5.5-N-hydroxysuccinimide (NHS)] and a green dye (fluorescein-NHS). In vivo fluorescence i...
Gespeichert in:
| Veröffentlicht in: | Oncology letters 2015-03, Vol.9 (3), p.1231-1235 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1235 |
|---|---|
| container_issue | 3 |
| container_start_page | 1231 |
| container_title | Oncology letters |
| container_volume | 9 |
| creator | ZHANG, QIONG WANG, FAN WU, YAO-SEN ZHANG, KE-KE LIN, YAN ZHU, XUE-QIONG LV, JIE-QIANG LU, XIAO-SHENG ZHANG, XIAO-LEI HU, YUE HUANG, YIN-PING |
| description | To investigate the feasibility of the anti-mucin 1 (anti-MUC1/CD227) antibody in the fluorescent imaging of ovarian cancer, the CD227 antibody and a control IgG antibody were labeled with a near-infrared dye [Cy5.5-N-hydroxysuccinimide (NHS)] and a green dye (fluorescein-NHS). In vivo fluorescence images were obtained at 4, 12 and 36 h after injection of the probes into OVCAR3 tumor-bearing mice. The tumor to background ratios were calculated for both probes. Ex vivo fluorescence images were obtained following sacrifice at 36 h. After conjugation to Cy5.5 and fluorescein, the dual-color labeled CD227 probe (Ab-FL-Cy5.5) could be visualized by both green and near-infrared fluorescence. Uptake by the tumors was higher for the Ab-FL-Cy5.5 than for the IgG-Cy5.5 probe. All tumors could be visualized by in vivo imaging with an acceptable tumor to background ratio. Ex vivo studies demonstrated the advantages of using green fluorescence imaging to guide the resection of tumor tissues. These preliminary data indicate that the Ab-FL-Cy5.5 probe is promising for further tumor imaging applications and clinical translation. |
| doi_str_mv | 10.3892/ol.2014.2807 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4315008</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A408647332</galeid><sourcerecordid>A408647332</sourcerecordid><originalsourceid>FETCH-LOGICAL-c607t-34c16daaa291d5e22feeaa40172b0461a72fec6e72f2a1dfc2d9342f6ccb72053</originalsourceid><addsrcrecordid>eNptks9vFCEUxydGY5vam2dDYmI8OCs_ZoDx0GRTfyZNvOiZvAFml4aBFWaa7H8vY-tu1wgH4PF53wcv36p6SfCKyY6-j35FMWlWVGLxpDonoqM1wZI-PexFc1Zd5nyLy2g5kZI_r85oyzmTUp5X_ccZfK2jjwl56K23BkGYXD3O2gVE_hz6aPZoKIQbYePCBsUBxTtIDgLSELRNH9Aa7ZL1bnQB0r5kFdSjPM1m_6J6NoDP9vJhvah-fv704_prffP9y7fr9U2tORZTzRpNuAEA2hHTWkoHawEaTATtccMJiBLR3JaFAjGDpqZjDR241r2guGUX1dW97m7uR2u0DVMCr3apPCXtVQSnTm-C26pNvFMNIy3Gsgi8fRBI8dds86RGl7X1HoKNc1ZEUs7JQhf09T_obZxTKN9TpGMFo52gR2oD3ioXhljq6kVUrRsseSMYW6jVf6gyjR2djsEOrsRPEt48Stha8NM2Rz9PLoZ8Cr67B3WKOSc7HJpBsFr8o6JXi3_U4p-Cv3rcwAP81y3HwnkHwTgT87G7vsZdjVlNKCPsN8wCy0E</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1932662972</pqid></control><display><type>article</type><title>Dual-color labeled anti-mucin 1 antibody for imaging of ovarian cancer: A preliminary animal study</title><source>Spandidos Publications Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>ZHANG, QIONG ; WANG, FAN ; WU, YAO-SEN ; ZHANG, KE-KE ; LIN, YAN ; ZHU, XUE-QIONG ; LV, JIE-QIANG ; LU, XIAO-SHENG ; ZHANG, XIAO-LEI ; HU, YUE ; HUANG, YIN-PING</creator><creatorcontrib>ZHANG, QIONG ; WANG, FAN ; WU, YAO-SEN ; ZHANG, KE-KE ; LIN, YAN ; ZHU, XUE-QIONG ; LV, JIE-QIANG ; LU, XIAO-SHENG ; ZHANG, XIAO-LEI ; HU, YUE ; HUANG, YIN-PING</creatorcontrib><description>To investigate the feasibility of the anti-mucin 1 (anti-MUC1/CD227) antibody in the fluorescent imaging of ovarian cancer, the CD227 antibody and a control IgG antibody were labeled with a near-infrared dye [Cy5.5-N-hydroxysuccinimide (NHS)] and a green dye (fluorescein-NHS). In vivo fluorescence images were obtained at 4, 12 and 36 h after injection of the probes into OVCAR3 tumor-bearing mice. The tumor to background ratios were calculated for both probes. Ex vivo fluorescence images were obtained following sacrifice at 36 h. After conjugation to Cy5.5 and fluorescein, the dual-color labeled CD227 probe (Ab-FL-Cy5.5) could be visualized by both green and near-infrared fluorescence. Uptake by the tumors was higher for the Ab-FL-Cy5.5 than for the IgG-Cy5.5 probe. All tumors could be visualized by in vivo imaging with an acceptable tumor to background ratio. Ex vivo studies demonstrated the advantages of using green fluorescence imaging to guide the resection of tumor tissues. These preliminary data indicate that the Ab-FL-Cy5.5 probe is promising for further tumor imaging applications and clinical translation.</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2014.2807</identifier><identifier>PMID: 25663888</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Cancer ; CD227 antibody ; Cell culture ; Chemokines ; Dyes ; FDA approval ; Fluorescence ; fluorescence imaging ; Growth factors ; Immunoglobulins ; Medical imaging ; Medical prognosis ; mucin 1 antibody ; near-infrared fluorescence ; NMR ; Nuclear magnetic resonance ; Oncology ; Oncology, Experimental ; Ovarian cancer ; Studies ; Surgery ; Tumors ; Vitamin B</subject><ispartof>Oncology letters, 2015-03, Vol.9 (3), p.1231-1235</ispartof><rights>Copyright © 2015, Spandidos Publications</rights><rights>COPYRIGHT 2015 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2015</rights><rights>Copyright © 2015, Spandidos Publications 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c607t-34c16daaa291d5e22feeaa40172b0461a72fec6e72f2a1dfc2d9342f6ccb72053</citedby><cites>FETCH-LOGICAL-c607t-34c16daaa291d5e22feeaa40172b0461a72fec6e72f2a1dfc2d9342f6ccb72053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4315008/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4315008/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,5572,27926,27927,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25663888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ZHANG, QIONG</creatorcontrib><creatorcontrib>WANG, FAN</creatorcontrib><creatorcontrib>WU, YAO-SEN</creatorcontrib><creatorcontrib>ZHANG, KE-KE</creatorcontrib><creatorcontrib>LIN, YAN</creatorcontrib><creatorcontrib>ZHU, XUE-QIONG</creatorcontrib><creatorcontrib>LV, JIE-QIANG</creatorcontrib><creatorcontrib>LU, XIAO-SHENG</creatorcontrib><creatorcontrib>ZHANG, XIAO-LEI</creatorcontrib><creatorcontrib>HU, YUE</creatorcontrib><creatorcontrib>HUANG, YIN-PING</creatorcontrib><title>Dual-color labeled anti-mucin 1 antibody for imaging of ovarian cancer: A preliminary animal study</title><title>Oncology letters</title><addtitle>Oncol Lett</addtitle><description>To investigate the feasibility of the anti-mucin 1 (anti-MUC1/CD227) antibody in the fluorescent imaging of ovarian cancer, the CD227 antibody and a control IgG antibody were labeled with a near-infrared dye [Cy5.5-N-hydroxysuccinimide (NHS)] and a green dye (fluorescein-NHS). In vivo fluorescence images were obtained at 4, 12 and 36 h after injection of the probes into OVCAR3 tumor-bearing mice. The tumor to background ratios were calculated for both probes. Ex vivo fluorescence images were obtained following sacrifice at 36 h. After conjugation to Cy5.5 and fluorescein, the dual-color labeled CD227 probe (Ab-FL-Cy5.5) could be visualized by both green and near-infrared fluorescence. Uptake by the tumors was higher for the Ab-FL-Cy5.5 than for the IgG-Cy5.5 probe. All tumors could be visualized by in vivo imaging with an acceptable tumor to background ratio. Ex vivo studies demonstrated the advantages of using green fluorescence imaging to guide the resection of tumor tissues. These preliminary data indicate that the Ab-FL-Cy5.5 probe is promising for further tumor imaging applications and clinical translation.</description><subject>Cancer</subject><subject>CD227 antibody</subject><subject>Cell culture</subject><subject>Chemokines</subject><subject>Dyes</subject><subject>FDA approval</subject><subject>Fluorescence</subject><subject>fluorescence imaging</subject><subject>Growth factors</subject><subject>Immunoglobulins</subject><subject>Medical imaging</subject><subject>Medical prognosis</subject><subject>mucin 1 antibody</subject><subject>near-infrared fluorescence</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Oncology</subject><subject>Oncology, Experimental</subject><subject>Ovarian cancer</subject><subject>Studies</subject><subject>Surgery</subject><subject>Tumors</subject><subject>Vitamin B</subject><issn>1792-1074</issn><issn>1792-1082</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptks9vFCEUxydGY5vam2dDYmI8OCs_ZoDx0GRTfyZNvOiZvAFml4aBFWaa7H8vY-tu1wgH4PF53wcv36p6SfCKyY6-j35FMWlWVGLxpDonoqM1wZI-PexFc1Zd5nyLy2g5kZI_r85oyzmTUp5X_ccZfK2jjwl56K23BkGYXD3O2gVE_hz6aPZoKIQbYePCBsUBxTtIDgLSELRNH9Aa7ZL1bnQB0r5kFdSjPM1m_6J6NoDP9vJhvah-fv704_prffP9y7fr9U2tORZTzRpNuAEA2hHTWkoHawEaTATtccMJiBLR3JaFAjGDpqZjDR241r2guGUX1dW97m7uR2u0DVMCr3apPCXtVQSnTm-C26pNvFMNIy3Gsgi8fRBI8dds86RGl7X1HoKNc1ZEUs7JQhf09T_obZxTKN9TpGMFo52gR2oD3ioXhljq6kVUrRsseSMYW6jVf6gyjR2djsEOrsRPEt48Stha8NM2Rz9PLoZ8Cr67B3WKOSc7HJpBsFr8o6JXi3_U4p-Cv3rcwAP81y3HwnkHwTgT87G7vsZdjVlNKCPsN8wCy0E</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>ZHANG, QIONG</creator><creator>WANG, FAN</creator><creator>WU, YAO-SEN</creator><creator>ZHANG, KE-KE</creator><creator>LIN, YAN</creator><creator>ZHU, XUE-QIONG</creator><creator>LV, JIE-QIANG</creator><creator>LU, XIAO-SHENG</creator><creator>ZHANG, XIAO-LEI</creator><creator>HU, YUE</creator><creator>HUANG, YIN-PING</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150301</creationdate><title>Dual-color labeled anti-mucin 1 antibody for imaging of ovarian cancer: A preliminary animal study</title><author>ZHANG, QIONG ; WANG, FAN ; WU, YAO-SEN ; ZHANG, KE-KE ; LIN, YAN ; ZHU, XUE-QIONG ; LV, JIE-QIANG ; LU, XIAO-SHENG ; ZHANG, XIAO-LEI ; HU, YUE ; HUANG, YIN-PING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c607t-34c16daaa291d5e22feeaa40172b0461a72fec6e72f2a1dfc2d9342f6ccb72053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Cancer</topic><topic>CD227 antibody</topic><topic>Cell culture</topic><topic>Chemokines</topic><topic>Dyes</topic><topic>FDA approval</topic><topic>Fluorescence</topic><topic>fluorescence imaging</topic><topic>Growth factors</topic><topic>Immunoglobulins</topic><topic>Medical imaging</topic><topic>Medical prognosis</topic><topic>mucin 1 antibody</topic><topic>near-infrared fluorescence</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Oncology</topic><topic>Oncology, Experimental</topic><topic>Ovarian cancer</topic><topic>Studies</topic><topic>Surgery</topic><topic>Tumors</topic><topic>Vitamin B</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ZHANG, QIONG</creatorcontrib><creatorcontrib>WANG, FAN</creatorcontrib><creatorcontrib>WU, YAO-SEN</creatorcontrib><creatorcontrib>ZHANG, KE-KE</creatorcontrib><creatorcontrib>LIN, YAN</creatorcontrib><creatorcontrib>ZHU, XUE-QIONG</creatorcontrib><creatorcontrib>LV, JIE-QIANG</creatorcontrib><creatorcontrib>LU, XIAO-SHENG</creatorcontrib><creatorcontrib>ZHANG, XIAO-LEI</creatorcontrib><creatorcontrib>HU, YUE</creatorcontrib><creatorcontrib>HUANG, YIN-PING</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ZHANG, QIONG</au><au>WANG, FAN</au><au>WU, YAO-SEN</au><au>ZHANG, KE-KE</au><au>LIN, YAN</au><au>ZHU, XUE-QIONG</au><au>LV, JIE-QIANG</au><au>LU, XIAO-SHENG</au><au>ZHANG, XIAO-LEI</au><au>HU, YUE</au><au>HUANG, YIN-PING</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual-color labeled anti-mucin 1 antibody for imaging of ovarian cancer: A preliminary animal study</atitle><jtitle>Oncology letters</jtitle><addtitle>Oncol Lett</addtitle><date>2015-03-01</date><risdate>2015</risdate><volume>9</volume><issue>3</issue><spage>1231</spage><epage>1235</epage><pages>1231-1235</pages><issn>1792-1074</issn><eissn>1792-1082</eissn><abstract>To investigate the feasibility of the anti-mucin 1 (anti-MUC1/CD227) antibody in the fluorescent imaging of ovarian cancer, the CD227 antibody and a control IgG antibody were labeled with a near-infrared dye [Cy5.5-N-hydroxysuccinimide (NHS)] and a green dye (fluorescein-NHS). In vivo fluorescence images were obtained at 4, 12 and 36 h after injection of the probes into OVCAR3 tumor-bearing mice. The tumor to background ratios were calculated for both probes. Ex vivo fluorescence images were obtained following sacrifice at 36 h. After conjugation to Cy5.5 and fluorescein, the dual-color labeled CD227 probe (Ab-FL-Cy5.5) could be visualized by both green and near-infrared fluorescence. Uptake by the tumors was higher for the Ab-FL-Cy5.5 than for the IgG-Cy5.5 probe. All tumors could be visualized by in vivo imaging with an acceptable tumor to background ratio. Ex vivo studies demonstrated the advantages of using green fluorescence imaging to guide the resection of tumor tissues. These preliminary data indicate that the Ab-FL-Cy5.5 probe is promising for further tumor imaging applications and clinical translation.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>25663888</pmid><doi>10.3892/ol.2014.2807</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1792-1074 |
| ispartof | Oncology letters, 2015-03, Vol.9 (3), p.1231-1235 |
| issn | 1792-1074 1792-1082 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4315008 |
| source | Spandidos Publications Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Cancer CD227 antibody Cell culture Chemokines Dyes FDA approval Fluorescence fluorescence imaging Growth factors Immunoglobulins Medical imaging Medical prognosis mucin 1 antibody near-infrared fluorescence NMR Nuclear magnetic resonance Oncology Oncology, Experimental Ovarian cancer Studies Surgery Tumors Vitamin B |
| title | Dual-color labeled anti-mucin 1 antibody for imaging of ovarian cancer: A preliminary animal study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T07%3A15%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dual-color%20labeled%20anti-mucin%201%20antibody%20for%20imaging%20of%20ovarian%20cancer:%20A%20preliminary%20animal%20study&rft.jtitle=Oncology%20letters&rft.au=ZHANG,%20QIONG&rft.date=2015-03-01&rft.volume=9&rft.issue=3&rft.spage=1231&rft.epage=1235&rft.pages=1231-1235&rft.issn=1792-1074&rft.eissn=1792-1082&rft_id=info:doi/10.3892/ol.2014.2807&rft_dat=%3Cgale_pubme%3EA408647332%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1932662972&rft_id=info:pmid/25663888&rft_galeid=A408647332&rfr_iscdi=true |