Renal F4/80+ CD11c+ mononuclear phagocytes display phenotypic and functional characteristics of macrophages in health and in adriamycin nephropathy
Conventional markers of macrophages (Mфs) and dendritic cells (DCs) lack specificity and often overlap, leading to confusion and controversy regarding the precise function of these cells in kidney and other diseases. This study aimed to identify the phenotype and function of renal mononuclear phagoc...
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| Veröffentlicht in: | Journal of the American Society of Nephrology 2015-02, Vol.26 (2), p.349-363 |
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| creator | Cao, Qi Wang, Yiping Wang, Xin Maggie Lu, Junyu Lee, Vincent W S Ye, Qianling Nguyen, Hanh Zheng, Guoping Zhao, Ye Alexander, Stephen I Harris, David C H |
| description | Conventional markers of macrophages (Mфs) and dendritic cells (DCs) lack specificity and often overlap, leading to confusion and controversy regarding the precise function of these cells in kidney and other diseases. This study aimed to identify the phenotype and function of renal mononuclear phagocytes (rMPs) expressing key markers of both Mфs and DCs. F4/80(+)CD11c(+) cells accounted for 45% of total rMPs in normal kidneys and in those from mice with Adriamycin nephropathy (AN). Despite expression of the DC marker CD11c, these double-positive rMPs displayed the features of Mфs, including Mф-like morphology, high expression of CD68, CD204, and CD206, and high phagocytic ability but low antigen-presenting ability. F4/80(+)CD11c(+) cells were found in the cortex but not in the medulla of the kidney. In AN, F4/80(+)CD11c(+) cells displayed an M1 Mф phenotype with high expression of inflammatory mediators and costimulatory factors. Adoptive transfer of F4/80(+)CD11c(+) cells separated from diseased kidney aggravated renal injury in AN mice. Furthermore, adoptive transfer of common progenitors revealed that kidney F4/80(+)CD11c(+) cells were derived predominantly from monocytes, but not from pre-DCs. In conclusion, renal F4/80(+)CD11c(+) cells are a major subset of rMPs and display Mф-like phenotypic and functional characteristics in health and in AN. |
| doi_str_mv | 10.1681/asn.2013121336 |
| format | Article |
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This study aimed to identify the phenotype and function of renal mononuclear phagocytes (rMPs) expressing key markers of both Mфs and DCs. F4/80(+)CD11c(+) cells accounted for 45% of total rMPs in normal kidneys and in those from mice with Adriamycin nephropathy (AN). Despite expression of the DC marker CD11c, these double-positive rMPs displayed the features of Mфs, including Mф-like morphology, high expression of CD68, CD204, and CD206, and high phagocytic ability but low antigen-presenting ability. F4/80(+)CD11c(+) cells were found in the cortex but not in the medulla of the kidney. In AN, F4/80(+)CD11c(+) cells displayed an M1 Mф phenotype with high expression of inflammatory mediators and costimulatory factors. Adoptive transfer of F4/80(+)CD11c(+) cells separated from diseased kidney aggravated renal injury in AN mice. Furthermore, adoptive transfer of common progenitors revealed that kidney F4/80(+)CD11c(+) cells were derived predominantly from monocytes, but not from pre-DCs. In conclusion, renal F4/80(+)CD11c(+) cells are a major subset of rMPs and display Mф-like phenotypic and functional characteristics in health and in AN.</description><identifier>ISSN: 1046-6673</identifier><identifier>EISSN: 1533-3450</identifier><identifier>DOI: 10.1681/asn.2013121336</identifier><identifier>PMID: 25012165</identifier><language>eng</language><publisher>United States: American Society of Nephrology</publisher><subject>Adoptive Transfer ; Animals ; Antigens, CD - metabolism ; Antigens, Differentiation - metabolism ; Antigens, Differentiation, Myelomonocytic - metabolism ; Basic Research ; Biomarkers - metabolism ; CD11 Antigens - metabolism ; Disease Models, Animal ; Doxorubicin - adverse effects ; In Vitro Techniques ; Kidney - pathology ; Kidney - physiology ; Kidney Diseases - chemically induced ; Kidney Diseases - pathology ; Kidney Diseases - physiopathology ; Lectins, C-Type - metabolism ; Macrophages - immunology ; Macrophages - pathology ; Macrophages - physiology ; Mannose-Binding Lectins - metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Phagocytes - immunology ; Phagocytes - pathology ; Phagocytes - physiology ; Phenotype ; Receptors, Cell Surface - metabolism ; Scavenger Receptors, Class A - metabolism</subject><ispartof>Journal of the American Society of Nephrology, 2015-02, Vol.26 (2), p.349-363</ispartof><rights>Copyright © 2015 by the American Society of Nephrology.</rights><rights>Copyright © 2015 by the American Society of Nephrology 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-59c407f64919e45c6dbdf7e6349d08d2706211e53847d293ae090b5a0d63c10d3</citedby><cites>FETCH-LOGICAL-c386t-59c407f64919e45c6dbdf7e6349d08d2706211e53847d293ae090b5a0d63c10d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310657/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310657/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25012165$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cao, Qi</creatorcontrib><creatorcontrib>Wang, Yiping</creatorcontrib><creatorcontrib>Wang, Xin Maggie</creatorcontrib><creatorcontrib>Lu, Junyu</creatorcontrib><creatorcontrib>Lee, Vincent W S</creatorcontrib><creatorcontrib>Ye, Qianling</creatorcontrib><creatorcontrib>Nguyen, Hanh</creatorcontrib><creatorcontrib>Zheng, Guoping</creatorcontrib><creatorcontrib>Zhao, Ye</creatorcontrib><creatorcontrib>Alexander, Stephen I</creatorcontrib><creatorcontrib>Harris, David C H</creatorcontrib><title>Renal F4/80+ CD11c+ mononuclear phagocytes display phenotypic and functional characteristics of macrophages in health and in adriamycin nephropathy</title><title>Journal of the American Society of Nephrology</title><addtitle>J Am Soc Nephrol</addtitle><description>Conventional markers of macrophages (Mфs) and dendritic cells (DCs) lack specificity and often overlap, leading to confusion and controversy regarding the precise function of these cells in kidney and other diseases. This study aimed to identify the phenotype and function of renal mononuclear phagocytes (rMPs) expressing key markers of both Mфs and DCs. F4/80(+)CD11c(+) cells accounted for 45% of total rMPs in normal kidneys and in those from mice with Adriamycin nephropathy (AN). Despite expression of the DC marker CD11c, these double-positive rMPs displayed the features of Mфs, including Mф-like morphology, high expression of CD68, CD204, and CD206, and high phagocytic ability but low antigen-presenting ability. F4/80(+)CD11c(+) cells were found in the cortex but not in the medulla of the kidney. In AN, F4/80(+)CD11c(+) cells displayed an M1 Mф phenotype with high expression of inflammatory mediators and costimulatory factors. Adoptive transfer of F4/80(+)CD11c(+) cells separated from diseased kidney aggravated renal injury in AN mice. Furthermore, adoptive transfer of common progenitors revealed that kidney F4/80(+)CD11c(+) cells were derived predominantly from monocytes, but not from pre-DCs. In conclusion, renal F4/80(+)CD11c(+) cells are a major subset of rMPs and display Mф-like phenotypic and functional characteristics in health and in AN.</description><subject>Adoptive Transfer</subject><subject>Animals</subject><subject>Antigens, CD - metabolism</subject><subject>Antigens, Differentiation - metabolism</subject><subject>Antigens, Differentiation, Myelomonocytic - metabolism</subject><subject>Basic Research</subject><subject>Biomarkers - metabolism</subject><subject>CD11 Antigens - metabolism</subject><subject>Disease Models, Animal</subject><subject>Doxorubicin - adverse effects</subject><subject>In Vitro Techniques</subject><subject>Kidney - pathology</subject><subject>Kidney - physiology</subject><subject>Kidney Diseases - chemically induced</subject><subject>Kidney Diseases - pathology</subject><subject>Kidney Diseases - physiopathology</subject><subject>Lectins, C-Type - metabolism</subject><subject>Macrophages - immunology</subject><subject>Macrophages - pathology</subject><subject>Macrophages - physiology</subject><subject>Mannose-Binding Lectins - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Phagocytes - immunology</subject><subject>Phagocytes - pathology</subject><subject>Phagocytes - physiology</subject><subject>Phenotype</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Scavenger Receptors, Class A - metabolism</subject><issn>1046-6673</issn><issn>1533-3450</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1v1DAQhi0EoqVw5Yh8r7Idxx9JLkjVQgtSRaUC52jWdhqjxI5sb6X8Dv4w3hZKe5rP9xmNXkLeM9gw1bIzTH5TA-OsZpyrF-SYSc4rLiS8LDkIVSnV8CPyJqVfAEzWTfOaHNUSikDJY_L7xnqc6IU4a-GUbj8xpk_pHHzwez1ZjHQZ8TboNdtEjUvLhGtpWR_yujhN0Rs67L3OLhwwesSIOtvoUnY60TDQGXUMB0gBOE9Hi1Me73WlQhMdzqsuqbfLWBYxj-tb8mrAKdl3f-MJ-Xnx-cf2S3V1ffl1e35Vad6qXMlOC2gGJTrWWSG1MjszNFZx0RloTd2AqhmzkreiMXXH0UIHO4lgFNcMDD8hHx-4y343W6OtzxGnfoluxrj2AV3_fOLd2N-Gu15wBko2BbB5AJQXU4p2eNQy6A_29Offv_X_7SmCD08vPq7_84P_AdsijiA</recordid><startdate>20150201</startdate><enddate>20150201</enddate><creator>Cao, Qi</creator><creator>Wang, Yiping</creator><creator>Wang, Xin Maggie</creator><creator>Lu, Junyu</creator><creator>Lee, Vincent W S</creator><creator>Ye, Qianling</creator><creator>Nguyen, Hanh</creator><creator>Zheng, Guoping</creator><creator>Zhao, Ye</creator><creator>Alexander, Stephen I</creator><creator>Harris, David C H</creator><general>American Society of Nephrology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20150201</creationdate><title>Renal F4/80+ CD11c+ mononuclear phagocytes display phenotypic and functional characteristics of macrophages in health and in adriamycin nephropathy</title><author>Cao, Qi ; Wang, Yiping ; Wang, Xin Maggie ; Lu, Junyu ; Lee, Vincent W S ; Ye, Qianling ; Nguyen, Hanh ; Zheng, Guoping ; Zhao, Ye ; Alexander, Stephen I ; Harris, David C H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-59c407f64919e45c6dbdf7e6349d08d2706211e53847d293ae090b5a0d63c10d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adoptive Transfer</topic><topic>Animals</topic><topic>Antigens, CD - metabolism</topic><topic>Antigens, Differentiation - metabolism</topic><topic>Antigens, Differentiation, Myelomonocytic - metabolism</topic><topic>Basic Research</topic><topic>Biomarkers - metabolism</topic><topic>CD11 Antigens - metabolism</topic><topic>Disease Models, Animal</topic><topic>Doxorubicin - adverse effects</topic><topic>In Vitro Techniques</topic><topic>Kidney - pathology</topic><topic>Kidney - physiology</topic><topic>Kidney Diseases - chemically induced</topic><topic>Kidney Diseases - pathology</topic><topic>Kidney Diseases - physiopathology</topic><topic>Lectins, C-Type - metabolism</topic><topic>Macrophages - immunology</topic><topic>Macrophages - pathology</topic><topic>Macrophages - physiology</topic><topic>Mannose-Binding Lectins - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Phagocytes - immunology</topic><topic>Phagocytes - pathology</topic><topic>Phagocytes - physiology</topic><topic>Phenotype</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Scavenger Receptors, Class A - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cao, Qi</creatorcontrib><creatorcontrib>Wang, Yiping</creatorcontrib><creatorcontrib>Wang, Xin Maggie</creatorcontrib><creatorcontrib>Lu, Junyu</creatorcontrib><creatorcontrib>Lee, Vincent W S</creatorcontrib><creatorcontrib>Ye, Qianling</creatorcontrib><creatorcontrib>Nguyen, Hanh</creatorcontrib><creatorcontrib>Zheng, Guoping</creatorcontrib><creatorcontrib>Zhao, Ye</creatorcontrib><creatorcontrib>Alexander, Stephen I</creatorcontrib><creatorcontrib>Harris, David C H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of the American Society of Nephrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cao, Qi</au><au>Wang, Yiping</au><au>Wang, Xin Maggie</au><au>Lu, Junyu</au><au>Lee, Vincent W S</au><au>Ye, Qianling</au><au>Nguyen, Hanh</au><au>Zheng, Guoping</au><au>Zhao, Ye</au><au>Alexander, Stephen I</au><au>Harris, David C H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Renal F4/80+ CD11c+ mononuclear phagocytes display phenotypic and functional characteristics of macrophages in health and in adriamycin nephropathy</atitle><jtitle>Journal of the American Society of Nephrology</jtitle><addtitle>J Am Soc Nephrol</addtitle><date>2015-02-01</date><risdate>2015</risdate><volume>26</volume><issue>2</issue><spage>349</spage><epage>363</epage><pages>349-363</pages><issn>1046-6673</issn><eissn>1533-3450</eissn><abstract>Conventional markers of macrophages (Mфs) and dendritic cells (DCs) lack specificity and often overlap, leading to confusion and controversy regarding the precise function of these cells in kidney and other diseases. This study aimed to identify the phenotype and function of renal mononuclear phagocytes (rMPs) expressing key markers of both Mфs and DCs. F4/80(+)CD11c(+) cells accounted for 45% of total rMPs in normal kidneys and in those from mice with Adriamycin nephropathy (AN). Despite expression of the DC marker CD11c, these double-positive rMPs displayed the features of Mфs, including Mф-like morphology, high expression of CD68, CD204, and CD206, and high phagocytic ability but low antigen-presenting ability. F4/80(+)CD11c(+) cells were found in the cortex but not in the medulla of the kidney. In AN, F4/80(+)CD11c(+) cells displayed an M1 Mф phenotype with high expression of inflammatory mediators and costimulatory factors. Adoptive transfer of F4/80(+)CD11c(+) cells separated from diseased kidney aggravated renal injury in AN mice. Furthermore, adoptive transfer of common progenitors revealed that kidney F4/80(+)CD11c(+) cells were derived predominantly from monocytes, but not from pre-DCs. In conclusion, renal F4/80(+)CD11c(+) cells are a major subset of rMPs and display Mф-like phenotypic and functional characteristics in health and in AN.</abstract><cop>United States</cop><pub>American Society of Nephrology</pub><pmid>25012165</pmid><doi>10.1681/asn.2013121336</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Adoptive Transfer Animals Antigens, CD - metabolism Antigens, Differentiation - metabolism Antigens, Differentiation, Myelomonocytic - metabolism Basic Research Biomarkers - metabolism CD11 Antigens - metabolism Disease Models, Animal Doxorubicin - adverse effects In Vitro Techniques Kidney - pathology Kidney - physiology Kidney Diseases - chemically induced Kidney Diseases - pathology Kidney Diseases - physiopathology Lectins, C-Type - metabolism Macrophages - immunology Macrophages - pathology Macrophages - physiology Mannose-Binding Lectins - metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL Phagocytes - immunology Phagocytes - pathology Phagocytes - physiology Phenotype Receptors, Cell Surface - metabolism Scavenger Receptors, Class A - metabolism |
| title | Renal F4/80+ CD11c+ mononuclear phagocytes display phenotypic and functional characteristics of macrophages in health and in adriamycin nephropathy |
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